xhoi xbai  (New England Biolabs)


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    Structured Review

    New England Biolabs xhoi xbai
    Xhoi Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi xbai/product/New England Biolabs
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xhoi xbai - by Bioz Stars, 2020-03
    77/100 stars

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    Clone Assay:

    Article Title: Genome-Wide Mapping of DNA Strand Breaks
    Article Snippet: .. The resulting DNA fragment containing an HO cleavage site was cloned into XhoI-XbaI (New England Biolabs) restriction sites of the YIp-PHO5 plasmid (obtained from Dr Luc Gaudreau, Université de Sherbrooke). .. The resulting YIp-PHO5-HO plasmid was linearized with SalI (New England Biolabs) and integrated into the PHO5 genomic locus of the bar1 Δ::HIS3 strain.

    Southern Blot:

    Article Title: Genome-Wide Mapping of DNA Strand Breaks
    Article Snippet: The resulting DNA fragment containing an HO cleavage site was cloned into XhoI-XbaI (New England Biolabs) restriction sites of the YIp-PHO5 plasmid (obtained from Dr Luc Gaudreau, Université de Sherbrooke). .. All integration and pop-out steps were confirmed by PCR and Southern blot analyses.

    Overlap Extension Polymerase Chain Reaction:

    Article Title: Genome-Wide Mapping of DNA Strand Breaks
    Article Snippet: The HO cleavage site 5′-AGTTTCAGCTTTCCGCAACAGTATAATTTTATAAA-3′ was integrated 45 nt downstream of the start codon of the PHO5 gene using the Splicing by Overlapping Extension PCR (SOE-PCR) technique . .. The resulting DNA fragment containing an HO cleavage site was cloned into XhoI-XbaI (New England Biolabs) restriction sites of the YIp-PHO5 plasmid (obtained from Dr Luc Gaudreau, Université de Sherbrooke).

    Plasmid Preparation:

    Article Title: Genome-Wide Mapping of DNA Strand Breaks
    Article Snippet: .. The resulting DNA fragment containing an HO cleavage site was cloned into XhoI-XbaI (New England Biolabs) restriction sites of the YIp-PHO5 plasmid (obtained from Dr Luc Gaudreau, Université de Sherbrooke). .. The resulting YIp-PHO5-HO plasmid was linearized with SalI (New England Biolabs) and integrated into the PHO5 genomic locus of the bar1 Δ::HIS3 strain.

    Polymerase Chain Reaction:

    Article Title: Genome-Wide Mapping of DNA Strand Breaks
    Article Snippet: The HO cleavage site 5′-AGTTTCAGCTTTCCGCAACAGTATAATTTTATAAA-3′ was integrated 45 nt downstream of the start codon of the PHO5 gene using the Splicing by Overlapping Extension PCR (SOE-PCR) technique . .. The resulting DNA fragment containing an HO cleavage site was cloned into XhoI-XbaI (New England Biolabs) restriction sites of the YIp-PHO5 plasmid (obtained from Dr Luc Gaudreau, Université de Sherbrooke).

    Derivative Assay:

    Article Title: Genome-Wide Mapping of DNA Strand Breaks
    Article Snippet: DFY046 This yeast strain DFY046 ( Mat a leu2-3,112 trp1-1 ura3-1 can1-100 ade2-1 his3-11,15 RAD5 bar1DELTA::KMX ) is derived from S288c background in which the BAR1 gene encoding a yeast protease was replaced by KMX using a classical PCR deletion technique . .. The resulting DNA fragment containing an HO cleavage site was cloned into XhoI-XbaI (New England Biolabs) restriction sites of the YIp-PHO5 plasmid (obtained from Dr Luc Gaudreau, Université de Sherbrooke).

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  • 77
    New England Biolabs xhoi xbai
    Xhoi Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi xbai/product/New England Biolabs
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    xhoi xbai - by Bioz Stars, 2020-03
    77/100 stars
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