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    Structured Review

    Thermo Fisher xhoi xbai sites
    Xhoi Xbai Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi xbai sites/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    xhoi xbai sites - by Bioz Stars, 2020-03
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Ras-Association Domain of Sorting Nexin 27 Is Critical for Regulating Expression of GIRK Potassium Channels
    Article Snippet: .. Molecular Biology For bimolecular fluorescence complementation (BiFC), the N-terminal half (amino acids 1 to 155) of the yellow fluorescent protein (N YFP or NY ) or the C-terminal half (amino acids 156 to 231) of YFP (C YFP or CY ) was cloned into XhoI-XbaI sites of pcDNA3.1+ vector (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: The miRNA expression vectors were cloned using PCR amplification of the relevant portions (generally speaking, this contains the putative miRNA site as well as ~100–150 bp flanking said site) of the viral genome (from genomic plasmids), followed by restriction enzyme digestion and ligation. .. Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen).

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: Second, two tandem copies of the IgG binding Z domain of protein A were amplified by PCR from pRAV-flag using primers 5′-CCC-GGG-ATG-AGG-TTA-ACC-ATG-GCG-CAA and 5′-GAG-CTC-TCT-AGA-TTA-CGC-GTC-TAC-TTT-CGG-CGC-CTG and cloned into SacI/SmaI sites of pBluescript-TEV. .. Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen).

    Amplification:

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: The miRNA expression vectors were cloned using PCR amplification of the relevant portions (generally speaking, this contains the putative miRNA site as well as ~100–150 bp flanking said site) of the viral genome (from genomic plasmids), followed by restriction enzyme digestion and ligation. .. Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen).

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The resulting plasmid was then inserted with the coding sequence of the Flag tag repeating three times at BamHI/XhoI sites.

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: .. c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. Ala/Glu mutations of Ser1186 and/or Ser1188 (Ser1186/1188) were introduced using a QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) with primers 12 to 21 ( ).

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: Second, two tandem copies of the IgG binding Z domain of protein A were amplified by PCR from pRAV-flag using primers 5′-CCC-GGG-ATG-AGG-TTA-ACC-ATG-GCG-CAA and 5′-GAG-CTC-TCT-AGA-TTA-CGC-GTC-TAC-TTT-CGG-CGC-CTG and cloned into SacI/SmaI sites of pBluescript-TEV. .. Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen).

    Construct:

    Article Title: CCR5 Levels and Expression Pattern Correlate with Infectability by Macrophage-tropic HIV-1, In Vitro
    Article Snippet: .. The PCR fragment was subcloned into the XhoI-XbaI sites of pCDNA3 (Invitrogen) and this construct was designated CCR5/pCDNA3. .. Another expression vector, CCR5/pMRB101, was also constructed in which the CCR5 cDNA was subcloned into the HindIII– XbaI sites of pMRB101 (kindly provided by Martin Robinson).

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen). .. The SV40 miRNA expression construct is as previously described [ ].

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: Mutated SF-1 constructs (mt1, mt2, dmt, mt3, and mt4 [see Fig. ]) were generated by PCR-based site-directed mutagenesis in pcDNA3-SF-1-HA. .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. To establish stable Tet-inducible cell lines, eIF4G-e and its variants containing c-myc and Flag tags were subcloned into pcDNA5/FRT/TO (Invitrogen) from the corresponding pcDNA3.1 expression constructs.

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: Paragraph title: DNA constructs ... Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen).

    Luciferase:

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen). .. Luciferase reporter plasmids were constructed using PCR amplification of the relevant portions of the viral genome (from genomic plasmids), followed by restriction enzyme digestion and ligation.

    Expressing:

    Article Title: CCR5 Levels and Expression Pattern Correlate with Infectability by Macrophage-tropic HIV-1, In Vitro
    Article Snippet: The PCR fragment was subcloned into the XhoI-XbaI sites of pCDNA3 (Invitrogen) and this construct was designated CCR5/pCDNA3. .. Another expression vector, CCR5/pMRB101, was also constructed in which the CCR5 cDNA was subcloned into the HindIII– XbaI sites of pMRB101 (kindly provided by Martin Robinson).

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: The miRNA expression vectors were cloned using PCR amplification of the relevant portions (generally speaking, this contains the putative miRNA site as well as ~100–150 bp flanking said site) of the viral genome (from genomic plasmids), followed by restriction enzyme digestion and ligation. .. Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen).

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. To establish stable Tet-inducible cell lines, eIF4G-e and its variants containing c-myc and Flag tags were subcloned into pcDNA5/FRT/TO (Invitrogen) from the corresponding pcDNA3.1 expression constructs.

    Modification:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: Mouse fibroblast NIH 3T3 and human kidney 293T cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum. .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    Ligation:

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: The miRNA expression vectors were cloned using PCR amplification of the relevant portions (generally speaking, this contains the putative miRNA site as well as ~100–150 bp flanking said site) of the viral genome (from genomic plasmids), followed by restriction enzyme digestion and ligation. .. Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen).

    Cell Culture:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: Paragraph title: Cell culture, plasmids, and recombinant proteins. ... The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    Generated:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The resulting plasmid was then inserted with the coding sequence of the Flag tag repeating three times at BamHI/XhoI sites.

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: .. c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. Ala/Glu mutations of Ser1186 and/or Ser1188 (Ser1186/1188) were introduced using a QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) with primers 12 to 21 ( ).

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen). .. The chicken CIB1 targeting vectors were generated by amplifying targeting arms from DT40 genomic DNA using primers 5′-GGC-CCG-TTT-TCG-TCC-CCC-GGA and 5′-ACG-GCA-CCT-CCG-TGC-GGG-AGC (1.2 kb left arm) and 5′-TCC-AGC-AGC-ATA-AGG-GGT-CCC and 5′-CTG-CAC-AGA-GCT-CGT-TCC-CCA (1.0 kb right arm).

    Polymerase Chain Reaction:

    Article Title: CCR5 Levels and Expression Pattern Correlate with Infectability by Macrophage-tropic HIV-1, In Vitro
    Article Snippet: .. The PCR fragment was subcloned into the XhoI-XbaI sites of pCDNA3 (Invitrogen) and this construct was designated CCR5/pCDNA3. .. Another expression vector, CCR5/pMRB101, was also constructed in which the CCR5 cDNA was subcloned into the HindIII– XbaI sites of pMRB101 (kindly provided by Martin Robinson).

    Article Title: Identification of virus-encoded microRNAs in divergent Papillomaviruses
    Article Snippet: The miRNA expression vectors were cloned using PCR amplification of the relevant portions (generally speaking, this contains the putative miRNA site as well as ~100–150 bp flanking said site) of the viral genome (from genomic plasmids), followed by restriction enzyme digestion and ligation. .. Specifically, the indicated regions of each viral genome listed in were inserted into the XhoI/XbaI sites of pcDNA3.1 (Invitrogen).

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The resulting plasmid was then inserted with the coding sequence of the Flag tag repeating three times at BamHI/XhoI sites.

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: .. c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. Ala/Glu mutations of Ser1186 and/or Ser1188 (Ser1186/1188) were introduced using a QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) with primers 12 to 21 ( ).

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: Second, two tandem copies of the IgG binding Z domain of protein A were amplified by PCR from pRAV-flag using primers 5′-CCC-GGG-ATG-AGG-TTA-ACC-ATG-GCG-CAA and 5′-GAG-CTC-TCT-AGA-TTA-CGC-GTC-TAC-TTT-CGG-CGC-CTG and cloned into SacI/SmaI sites of pBluescript-TEV. .. Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen).

    Affinity Purification:

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: The tandem affinity purification constructs, pAID-STZ and pEGFP-STZ (Strep, TEV, Z domain), were made as follows. .. Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen).

    Binding Assay:

    Article Title: The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination
    Article Snippet: Second, two tandem copies of the IgG binding Z domain of protein A were amplified by PCR from pRAV-flag using primers 5′-CCC-GGG-ATG-AGG-TTA-ACC-ATG-GCG-CAA and 5′-GAG-CTC-TCT-AGA-TTA-CGC-GTC-TAC-TTT-CGG-CGC-CTG and cloned into SacI/SmaI sites of pBluescript-TEV. .. Fifth, Strep-TEV-ZZ was subcloned into the XhoI/XbaI sites of pcDNA3.1(Invitrogen).

    Fluorescence:

    Article Title: Ras-Association Domain of Sorting Nexin 27 Is Critical for Regulating Expression of GIRK Potassium Channels
    Article Snippet: .. Molecular Biology For bimolecular fluorescence complementation (BiFC), the N-terminal half (amino acids 1 to 155) of the yellow fluorescent protein (N YFP or NY ) or the C-terminal half (amino acids 156 to 231) of YFP (C YFP or CY ) was cloned into XhoI-XbaI sites of pcDNA3.1+ vector (Invitrogen, Carlsbad, CA, USA). ..

    Mutagenesis:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: Mutated SF-1 constructs (mt1, mt2, dmt, mt3, and mt4 [see Fig. ]) were generated by PCR-based site-directed mutagenesis in pcDNA3-SF-1-HA. .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. Ala/Glu mutations of Ser1186 and/or Ser1188 (Ser1186/1188) were introduced using a QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) with primers 12 to 21 ( ).

    Subcloning:

    Article Title: Ras-Association Domain of Sorting Nexin 27 Is Critical for Regulating Expression of GIRK Potassium Channels
    Article Snippet: Molecular Biology For bimolecular fluorescence complementation (BiFC), the N-terminal half (amino acids 1 to 155) of the yellow fluorescent protein (N YFP or NY ) or the C-terminal half (amino acids 156 to 231) of YFP (C YFP or CY ) was cloned into XhoI-XbaI sites of pcDNA3.1+ vector (Invitrogen, Carlsbad, CA, USA). .. A C-terminal fragment containing the RA domain of SNX27b (Asp272-Tyr526) was fused to GST by sub cloning into EcoRI and BamHI sites of pGEX-2T vector (GE Biosciences).

    Bimolecular Fluorescence Complementation Assay:

    Article Title: Ras-Association Domain of Sorting Nexin 27 Is Critical for Regulating Expression of GIRK Potassium Channels
    Article Snippet: .. Molecular Biology For bimolecular fluorescence complementation (BiFC), the N-terminal half (amino acids 1 to 155) of the yellow fluorescent protein (N YFP or NY ) or the C-terminal half (amino acids 156 to 231) of YFP (C YFP or CY ) was cloned into XhoI-XbaI sites of pcDNA3.1+ vector (Invitrogen, Carlsbad, CA, USA). ..

    Purification:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The recombinant proteins GST-FP, His-p300 HAT, and GST-PCAF HAT (where GST is glutathione S -transferase) were overexpressed in Escherichia coli strain BL21(DE3) pLys and purified as described previously ( ).

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. A recombinant glutathione S -transferase (GST)-tagged C-terminal fragment of eIF4G1 (GST-Ct) was purified as described before ( ).

    Sequencing:

    Article Title: CCR5 Levels and Expression Pattern Correlate with Infectability by Macrophage-tropic HIV-1, In Vitro
    Article Snippet: The PCR fragment was subcloned into the XhoI-XbaI sites of pCDNA3 (Invitrogen) and this construct was designated CCR5/pCDNA3. .. PCR fragments were sequenced to ascertain the sequence fidelity.

    Article Title: Ras-Association Domain of Sorting Nexin 27 Is Critical for Regulating Expression of GIRK Potassium Channels
    Article Snippet: Molecular Biology For bimolecular fluorescence complementation (BiFC), the N-terminal half (amino acids 1 to 155) of the yellow fluorescent protein (N YFP or NY ) or the C-terminal half (amino acids 156 to 231) of YFP (C YFP or CY ) was cloned into XhoI-XbaI sites of pcDNA3.1+ vector (Invitrogen, Carlsbad, CA, USA). .. The cDNAs for H-Ras (G12V & S17N) were obtained from Missouri S & T cDNA Resource Center (#RASH0000C0, http://www.cdna.org ) and subcloned into the pHis8-3 vector downstream of His8 tag sequence.

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The resulting plasmid was then inserted with the coding sequence of the Flag tag repeating three times at BamHI/XhoI sites.

    Plasmid Preparation:

    Article Title: CCR5 Levels and Expression Pattern Correlate with Infectability by Macrophage-tropic HIV-1, In Vitro
    Article Snippet: The PCR fragment was subcloned into the XhoI-XbaI sites of pCDNA3 (Invitrogen) and this construct was designated CCR5/pCDNA3. .. Another expression vector, CCR5/pMRB101, was also constructed in which the CCR5 cDNA was subcloned into the HindIII– XbaI sites of pMRB101 (kindly provided by Martin Robinson).

    Article Title: Ras-Association Domain of Sorting Nexin 27 Is Critical for Regulating Expression of GIRK Potassium Channels
    Article Snippet: .. Molecular Biology For bimolecular fluorescence complementation (BiFC), the N-terminal half (amino acids 1 to 155) of the yellow fluorescent protein (N YFP or NY ) or the C-terminal half (amino acids 156 to 231) of YFP (C YFP or CY ) was cloned into XhoI-XbaI sites of pcDNA3.1+ vector (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The resulting plasmid was then inserted with the coding sequence of the Flag tag repeating three times at BamHI/XhoI sites.

    Positron Emission Tomography:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: The following constructs have previously been described: phscc2.3k-LacZ, pGEX-SF-1-FP , pGal4-SF-1 , pET-p300 HAT , pGEX5X-PCAT-HAT , pCMV-p300-myc WT and pCMV-p300-myc DY , pcDNA3.1-SF-1-HA , and pcDNA3-FLAG-GCN5 ( ). .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    FLAG-tag:

    Article Title: CCR5 Levels and Expression Pattern Correlate with Infectability by Macrophage-tropic HIV-1, In Vitro
    Article Snippet: For one set of transfectants, the 5′-primer also contained a Flag epitope (Asp.Tyr.Lys.Asp.Asp.Asp.Asp.Lys). .. The PCR fragment was subcloned into the XhoI-XbaI sites of pCDNA3 (Invitrogen) and this construct was designated CCR5/pCDNA3.

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen). .. The resulting plasmid was then inserted with the coding sequence of the Flag tag repeating three times at BamHI/XhoI sites.

    HAT Assay:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: The following constructs have previously been described: phscc2.3k-LacZ, pGEX-SF-1-FP , pGal4-SF-1 , pET-p300 HAT , pGEX5X-PCAT-HAT , pCMV-p300-myc WT and pCMV-p300-myc DY , pcDNA3.1-SF-1-HA , and pcDNA3-FLAG-GCN5 ( ). .. The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    Recombinant:

    Article Title: SF-1 (Nuclear Receptor 5A1) Activity Is Activated by Cyclic AMP via p300-Mediated Recruitment to Active Foci, Acetylation, and Increased DNA Binding
    Article Snippet: Paragraph title: Cell culture, plasmids, and recombinant proteins. ... The plasmid pcDNA5-3xFLAG-SF-1 was generated by inserting the coding sequence of SF-1, amplified by PCR from pcDNA3-SF-1-HA, into XhoI/XbaI sites of pcDNA5/TO (Invitrogen).

    Article Title: Phosphorylation of Eukaryotic Translation Initiation Factor 4G1 (eIF4G1) by Protein Kinase C? Regulates eIF4G1 Binding to Mnk1 ▿
    Article Snippet: c-myc and Flag epitopes were inserted into NheI/HinDIII and XhoI/XbaI sites of pcDNA3.1 (Invitrogen) using annealed complementary oligonucleotide pairs 1/2 and 3/4, respectively ( ). eIF4G truncation variants were generated by PCR amplification of corresponding fragments with primers 5 to 11 (see A) (primers are listed in ), which were ligated into the HinDIII and XhoI sites of the dual-tag cassette. .. A recombinant glutathione S -transferase (GST)-tagged C-terminal fragment of eIF4G1 (GST-Ct) was purified as described before ( ).