xhoi noti  (Thermo Fisher)


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    Structured Review

    Thermo Fisher xhoi noti
    Xhoi Noti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi noti/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    xhoi noti - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: Single clones were used for infection experiments and phenotypic characterization. .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: .. The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas). .. The resulting fragment was cloned into the pCAG vector (kindly provided by Dr. Peter W. Andrews, University of Sheffield, Sheffield, UK).

    Amplification:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: Briefly, a non-polar kanamycin resistance ( aphA ) cassette ( ) and ~1 kb upstream and downstream “arms” for each gene were PCR amplified using primers ( ) with compatible 5′ restriction sites. .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: Using this construct as the template, the A4GALT coding region was amplified with primers PkXho For and PkNot Rev, which introduced restriction sites into the resulting product. .. The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas).

    Knock-Out:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: Paragraph title: Generation of H. pylori Isogenic Knockout Mutants ... All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Polymerase Chain Reaction:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: Briefly, a non-polar kanamycin resistance ( aphA ) cassette ( ) and ~1 kb upstream and downstream “arms” for each gene were PCR amplified using primers ( ) with compatible 5′ restriction sites. .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: The coding region of the A4GALT gene was PCR-amplified from the genomic DNA of NOR-positive and NOR-negative donors and cloned into the pGEM-T easy vector (Promega, Madison, WI). .. The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas).

    Construct:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: J166 deletion mutants of recA , addA , and ruvC were constructed by allelic exchange using methods similar to those previously described ( ). .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: Using this construct as the template, the A4GALT coding region was amplified with primers PkXho For and PkNot Rev, which introduced restriction sites into the resulting product. .. The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas).

    Selection:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection. .. Each shuttle plasmid was verified by sequencing and transferred into H. pylori J166 using natural transformation with kanamycin selection, thereby deleting most of the coding region of each gene.

    Activity Assay:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: After natural transformation ( ) with the appropriate PCR product and selection on Cm-containing media, four to eight clones were evaluated by PCR to confirm replacement of the wild-type allele with the null allele; urease activity and flagella-based motility were also confirmed. .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Infection:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: Single clones were used for infection experiments and phenotypic characterization. .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection.

    Expressing:

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: Paragraph title: Construction of Expression Vectors ... The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas).

    Sequencing:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection. .. Each shuttle plasmid was verified by sequencing and transferred into H. pylori J166 using natural transformation with kanamycin selection, thereby deleting most of the coding region of each gene.

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: .. The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas). .. The resulting fragment was cloned into the pCAG vector (kindly provided by Dr. Peter W. Andrews, University of Sheffield, Sheffield, UK).

    Transformation Assay:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection. .. Each shuttle plasmid was verified by sequencing and transferred into H. pylori J166 using natural transformation with kanamycin selection, thereby deleting most of the coding region of each gene.

    Plasmid Preparation:

    Article Title: Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization
    Article Snippet: .. All three fragments were restricted with the appropriate enzymes, ligated with pBluescript SK(-) (Stratagene, La Jolla, CA) plasmid DNA which was previously digested with XhoI/NotI, and transformed into One Shot TOP10 competent E. coli (Invitrogen, Carlsbad, CA) with kanamycin selection. .. Each shuttle plasmid was verified by sequencing and transferred into H. pylori J166 using natural transformation with kanamycin selection, thereby deleting most of the coding region of each gene.

    Article Title: A Single Point Mutation in the Gene Encoding Gb3/CD77 Synthase Causes a Rare Inherited Polyagglutination Syndrome *
    Article Snippet: .. The complete sequence was cloned into the pGEM-T easy vector, which was then digested with XhoI/NotI (Fermentas). .. The resulting fragment was cloned into the pCAG vector (kindly provided by Dr. Peter W. Andrews, University of Sheffield, Sheffield, UK).

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  • News
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  • Team
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  • Contact
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  • 93
    Thermo Fisher noti xhoi
    Noti Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti xhoi/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    noti xhoi - by Bioz Stars, 2020-04
    93/100 stars
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