xhoi hindiii  (Thermo Fisher)


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    Structured Review

    Thermo Fisher xhoi hindiii
    Xhoi Hindiii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi hindiii/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    xhoi hindiii - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages
    Article Snippet: .. The PCR product containing relA was digested with XhoI-HindIII and cloned into the same sites on pBAD-HisA (Invitrogen). .. The PCR product containing ssrB was digested with XhoI-BamHI and cloned into the same sites on pFLAG-CTC (Sigma). pRelA and pSsrB expressed His6 -tagged RelA and SsrB-FLAG fusion protein, respectively.

    Article Title: Estrogen regulation of microcephaly genes and evolution of brain sexual dimorphism in primates
    Article Snippet: .. The amplified DNA fragment was digested with XhoI/HinDIII and KpnI/NheI (Fermentas, Hanover, MD) and cloned into the pGL3-basic firefly luciferase reporter vector (Promega, Madison, WI) to construct human-aspm, chimpanzee-aspm, macaque-aspm, marmoset-aspm, human-cdk5rap2, chimapanzee-cdk5rap2, macaque-cdk5rap2, macrmoset-cdk5rap2, marmoset-MCPH1, human-wdr62, chimpanzee-wdr62, macaque-wdr62, marmoset-wdr62. .. The sequences of the cloned DNAs were verified by sequencing the entire region.

    Amplification:

    Article Title: Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages
    Article Snippet: To construct pRelA and pSsrB, the target genes were amplified by PCR with the following primers: relA-FW2 and relA-RV2 for relA and ssrB-FW and ssrB-RV for ssrB . .. The PCR product containing relA was digested with XhoI-HindIII and cloned into the same sites on pBAD-HisA (Invitrogen).

    Article Title: Estrogen regulation of microcephaly genes and evolution of brain sexual dimorphism in primates
    Article Snippet: .. The amplified DNA fragment was digested with XhoI/HinDIII and KpnI/NheI (Fermentas, Hanover, MD) and cloned into the pGL3-basic firefly luciferase reporter vector (Promega, Madison, WI) to construct human-aspm, chimpanzee-aspm, macaque-aspm, marmoset-aspm, human-cdk5rap2, chimapanzee-cdk5rap2, macaque-cdk5rap2, macrmoset-cdk5rap2, marmoset-MCPH1, human-wdr62, chimpanzee-wdr62, macaque-wdr62, marmoset-wdr62. .. The sequences of the cloned DNAs were verified by sequencing the entire region.

    Article Title: Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging
    Article Snippet: In brief, Ultramarine in pQE9N (Qiagen) and cjBlue in pRSET-B (Life Technologies) were amplified with a 5′ XhoI forward primer and a 3′ HindIII reverse primer using Taq polymerase (New England Biolabs) in the presence of MnCl2 . .. Full-length gene libraries were digested with XhoI/HindIII (Fermentas) and ligated into similarly digested pBAD/His B plasmid with T4 DNA ligase (Life Technologies).

    Mutagenesis:

    Article Title: Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging
    Article Snippet: Paragraph title: Random mutagenesis and E. coli plate preparation ... Full-length gene libraries were digested with XhoI/HindIII (Fermentas) and ligated into similarly digested pBAD/His B plasmid with T4 DNA ligase (Life Technologies).

    Construct:

    Article Title: Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages
    Article Snippet: To construct pRelA and pSsrB, the target genes were amplified by PCR with the following primers: relA-FW2 and relA-RV2 for relA and ssrB-FW and ssrB-RV for ssrB . .. The PCR product containing relA was digested with XhoI-HindIII and cloned into the same sites on pBAD-HisA (Invitrogen).

    Article Title: Estrogen regulation of microcephaly genes and evolution of brain sexual dimorphism in primates
    Article Snippet: .. The amplified DNA fragment was digested with XhoI/HinDIII and KpnI/NheI (Fermentas, Hanover, MD) and cloned into the pGL3-basic firefly luciferase reporter vector (Promega, Madison, WI) to construct human-aspm, chimpanzee-aspm, macaque-aspm, marmoset-aspm, human-cdk5rap2, chimapanzee-cdk5rap2, macaque-cdk5rap2, macrmoset-cdk5rap2, marmoset-MCPH1, human-wdr62, chimpanzee-wdr62, macaque-wdr62, marmoset-wdr62. .. The sequences of the cloned DNAs were verified by sequencing the entire region.

    Polymerase Chain Reaction:

    Article Title: Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages
    Article Snippet: .. The PCR product containing relA was digested with XhoI-HindIII and cloned into the same sites on pBAD-HisA (Invitrogen). .. The PCR product containing ssrB was digested with XhoI-BamHI and cloned into the same sites on pFLAG-CTC (Sigma). pRelA and pSsrB expressed His6 -tagged RelA and SsrB-FLAG fusion protein, respectively.

    Article Title: Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging
    Article Snippet: Random mutagenesis and E. coli plate preparation Random mutagenesis was performed by error-prone polymerase chain reaction (PCR) as described in Fromant et al. . .. Full-length gene libraries were digested with XhoI/HindIII (Fermentas) and ligated into similarly digested pBAD/His B plasmid with T4 DNA ligase (Life Technologies).

    Luciferase:

    Article Title: Estrogen regulation of microcephaly genes and evolution of brain sexual dimorphism in primates
    Article Snippet: .. The amplified DNA fragment was digested with XhoI/HinDIII and KpnI/NheI (Fermentas, Hanover, MD) and cloned into the pGL3-basic firefly luciferase reporter vector (Promega, Madison, WI) to construct human-aspm, chimpanzee-aspm, macaque-aspm, marmoset-aspm, human-cdk5rap2, chimapanzee-cdk5rap2, macaque-cdk5rap2, macrmoset-cdk5rap2, marmoset-MCPH1, human-wdr62, chimpanzee-wdr62, macaque-wdr62, marmoset-wdr62. .. The sequences of the cloned DNAs were verified by sequencing the entire region.

    Sequencing:

    Article Title: Estrogen regulation of microcephaly genes and evolution of brain sexual dimorphism in primates
    Article Snippet: The amplified DNA fragment was digested with XhoI/HinDIII and KpnI/NheI (Fermentas, Hanover, MD) and cloned into the pGL3-basic firefly luciferase reporter vector (Promega, Madison, WI) to construct human-aspm, chimpanzee-aspm, macaque-aspm, marmoset-aspm, human-cdk5rap2, chimapanzee-cdk5rap2, macaque-cdk5rap2, macrmoset-cdk5rap2, marmoset-MCPH1, human-wdr62, chimpanzee-wdr62, macaque-wdr62, marmoset-wdr62. .. The sequences of the cloned DNAs were verified by sequencing the entire region.

    Plasmid Preparation:

    Article Title: Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages
    Article Snippet: The PCR products were digested with BglII and XhoI, and cloned into the Bglll-XhoI site on pMW118 (Nippon Gene), generating plasmid pMW-Stm3169. .. The PCR product containing relA was digested with XhoI-HindIII and cloned into the same sites on pBAD-HisA (Invitrogen).

    Article Title: Estrogen regulation of microcephaly genes and evolution of brain sexual dimorphism in primates
    Article Snippet: .. The amplified DNA fragment was digested with XhoI/HinDIII and KpnI/NheI (Fermentas, Hanover, MD) and cloned into the pGL3-basic firefly luciferase reporter vector (Promega, Madison, WI) to construct human-aspm, chimpanzee-aspm, macaque-aspm, marmoset-aspm, human-cdk5rap2, chimapanzee-cdk5rap2, macaque-cdk5rap2, macrmoset-cdk5rap2, marmoset-MCPH1, human-wdr62, chimpanzee-wdr62, macaque-wdr62, marmoset-wdr62. .. The sequences of the cloned DNAs were verified by sequencing the entire region.

    Article Title: Engineering Dark Chromoprotein Reporters for Photoacoustic Microscopy and FRET Imaging
    Article Snippet: .. Full-length gene libraries were digested with XhoI/HindIII (Fermentas) and ligated into similarly digested pBAD/His B plasmid with T4 DNA ligase (Life Technologies). .. Plasmid libraries were expressed in E. coli strain DH10B (Life Technologies) on LB (Luria-Bertani) agar plates supplemented with 0.1 mg/ml ampicillin and 0.02% L-arabinose at 37 °C overnight.

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  • 99
    Thermo Fisher hindiii xhoi restriction enzymes
    Hindiii Xhoi Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hindiii xhoi restriction enzymes/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
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    Thermo Fisher bamhi aid ecori linker hindiii aid e58a xhoi cassettes
    Bamhi Aid Ecori Linker Hindiii Aid E58a Xhoi Cassettes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi aid ecori linker hindiii aid e58a xhoi cassettes/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bamhi aid ecori linker hindiii aid e58a xhoi cassettes - by Bioz Stars, 2020-04
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      Buy from Supplier

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