xhoi digested pcdna3 1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher xhoi digested pcdna3 1
    Xhoi Digested Pcdna3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi digested pcdna3 1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xhoi digested pcdna3 1 - by Bioz Stars, 2020-04
    85/100 stars

    Related Products / Commonly Used Together

    nhei
    pcr
    t4 dna ligase
    hindiii
    nontagged cdnas

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    Related Articles

    Clone Assay:

    Article Title: The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
    Article Snippet: .. The resulting PCR products for these nontagged cDNAs were cloned into HindIII- and XhoI-digested pcDNA3.1 (Invitrogen) and subsequently cloned into pCMV(4x)GFP after digestion with NheI and XbaI as green fluorescent protein (GFP)-tagged derivatives. ..

    Mutagenesis:

    Article Title: The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
    Article Snippet: All ie 1 mutants were constructed by PCR-driven mutagenesis with plasmid pON2202 ( ) as a template. pCMV IE1full-length and pCMV IE1full-length GFP were constructed from a cDNA generated by PCR with oligonucleotides 1 and 2 (Table ). pCMV IE11-420 and pCMV IE11-420 GFP were generated by PCR with oligonucleotides 1 and 3. pCMV IE11-479 and pCMV IE11-479 GFP were generated by PCR with oligonucleotides 1 and 4. .. The resulting PCR products for these nontagged cDNAs were cloned into HindIII- and XhoI-digested pcDNA3.1 (Invitrogen) and subsequently cloned into pCMV(4x)GFP after digestion with NheI and XbaI as green fluorescent protein (GFP)-tagged derivatives.

    Construct:

    Article Title: The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
    Article Snippet: All ie 1 mutants were constructed by PCR-driven mutagenesis with plasmid pON2202 ( ) as a template. pCMV IE1full-length and pCMV IE1full-length GFP were constructed from a cDNA generated by PCR with oligonucleotides 1 and 2 (Table ). pCMV IE11-420 and pCMV IE11-420 GFP were generated by PCR with oligonucleotides 1 and 3. pCMV IE11-479 and pCMV IE11-479 GFP were generated by PCR with oligonucleotides 1 and 4. .. The resulting PCR products for these nontagged cDNAs were cloned into HindIII- and XhoI-digested pcDNA3.1 (Invitrogen) and subsequently cloned into pCMV(4x)GFP after digestion with NheI and XbaI as green fluorescent protein (GFP)-tagged derivatives.

    Generated:

    Article Title: The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
    Article Snippet: All ie 1 mutants were constructed by PCR-driven mutagenesis with plasmid pON2202 ( ) as a template. pCMV IE1full-length and pCMV IE1full-length GFP were constructed from a cDNA generated by PCR with oligonucleotides 1 and 2 (Table ). pCMV IE11-420 and pCMV IE11-420 GFP were generated by PCR with oligonucleotides 1 and 3. pCMV IE11-479 and pCMV IE11-479 GFP were generated by PCR with oligonucleotides 1 and 4. .. The resulting PCR products for these nontagged cDNAs were cloned into HindIII- and XhoI-digested pcDNA3.1 (Invitrogen) and subsequently cloned into pCMV(4x)GFP after digestion with NheI and XbaI as green fluorescent protein (GFP)-tagged derivatives.

    Polymerase Chain Reaction:

    Article Title: The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
    Article Snippet: .. The resulting PCR products for these nontagged cDNAs were cloned into HindIII- and XhoI-digested pcDNA3.1 (Invitrogen) and subsequently cloned into pCMV(4x)GFP after digestion with NheI and XbaI as green fluorescent protein (GFP)-tagged derivatives. ..

    Plasmid Preparation:

    Article Title: The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
    Article Snippet: All ie 1 mutants were constructed by PCR-driven mutagenesis with plasmid pON2202 ( ) as a template. pCMV IE1full-length and pCMV IE1full-length GFP were constructed from a cDNA generated by PCR with oligonucleotides 1 and 2 (Table ). pCMV IE11-420 and pCMV IE11-420 GFP were generated by PCR with oligonucleotides 1 and 3. pCMV IE11-479 and pCMV IE11-479 GFP were generated by PCR with oligonucleotides 1 and 4. .. The resulting PCR products for these nontagged cDNAs were cloned into HindIII- and XhoI-digested pcDNA3.1 (Invitrogen) and subsequently cloned into pCMV(4x)GFP after digestion with NheI and XbaI as green fluorescent protein (GFP)-tagged derivatives.

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  • 80
    Thermo Fisher nhei xhoi digested pcdna3 1
    Nhei Xhoi Digested Pcdna3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei xhoi digested pcdna3 1/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhei xhoi digested pcdna3 1 - by Bioz Stars, 2020-04
    80/100 stars
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