xf ksr human embryonic stem cell hesc medium  (Thermo Fisher)


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    TaqMan OpenArray Human Embryonic Stem Cells Panel Configurable
    Description:
    The Embryonic Stem Cells array covers genes involved in maintenance of pluripotency and the self renewal status of embryonic stem cells ESC Included are embryonic stem cell specific genes that maintain their pluripotent and self renewal characteristics as well as key genes necessary for the first steps of induced Pluripotent Stem Cell generation The array also includes differentiation markers that can be used to monitor the early events of ESC differentiation
    Catalog Number:
    RVMFWWM
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    None
    Category:
    GX
    Format:
    Format 112 Openarray
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    Thermo Fisher xf ksr human embryonic stem cell hesc medium
    The Embryonic Stem Cells array covers genes involved in maintenance of pluripotency and the self renewal status of embryonic stem cells ESC Included are embryonic stem cell specific genes that maintain their pluripotent and self renewal characteristics as well as key genes necessary for the first steps of induced Pluripotent Stem Cell generation The array also includes differentiation markers that can be used to monitor the early events of ESC differentiation
    https://www.bioz.com/result/xf ksr human embryonic stem cell hesc medium/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xf ksr human embryonic stem cell hesc medium - by Bioz Stars, 2021-04
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: The fractions of hybridized probes were quantified from gels using ImageJ software. .. Cell culture Human Embryonic Stem Cells (hESC; HUES9, H7 and NKX2-5eGFP/w lines) were cultured on Matrigel-coated tissue culture polystyrene plates and maintained in Essential eight medium (Thermo Fisher). hESC medium was refreshed every 24 hr and hESCs were passaged at 70% confluency using 0.5 mM EDTA. .. U2OS cells were cultured in standard DMEM containing 10% FBS and 1% penicillin/streptomycin, and dissociated using TrypLE Express.

    Article Title: Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells
    Article Snippet: Due to the high complexity of the miRNAome, a genome-wide approach is mandatory for a better comprehension of the role these small non-coding RNAs have and functional studies that include all microRNAs members of families/clusters will be necessary to further understand their proper relevance in the differentiation process. .. Cell Culture and cell differentiation Human embryonic stem cells (H9-hTnnTZ-pGZ-D2) were obtained from WiCell and maintained in co-culture with irradiated primary mouse embryonic fibroblasts (MEFs) in the presence of FGF (8 ng/ml; Thermo Fisher Scientific) under conditions described by the supplier, as previously reported . .. This cell line has been genetically modified in WiCell to express both GFP and Zeocin resistance under the promoter of cardiac troponin T. All experiments were carried out in accordance with relevant guidelines and regulations.

    Article Title: Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency
    Article Snippet: Cell Culture Conventional (primed) human iPSC line C1 (Whitehead Institute Center for Human Stem Cell Research, Cambridge, MA) ( ) and human ESC lines WIBR2 and WIBR3 (Whitehead Institute Center for Human Stem Cell Research, Cambridge, MA) ( ) were maintained on mitomycin C inactivated MEF feeder layers and passaged mechanically using a drawn Pasteur pipette or enzymatically by treatment for 20 min with 1 mg/ml Collagenase type IV (GIBCO) followed by sequential sedimentation steps in human ESC medium (hESM) to remove single cells. .. Primed human ESCs and human iPSCs were cultured in hESM—DMEM/F12 (Invitrogen) supplemented with 15% FBS (Hyclone), 5% KSR (Invitrogen), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Invitrogen), penicillin-streptomycin (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma), and 4 ng/ml FGF2 (R & D systems). .. Naive human ESCs/hiPSCs were cultured on mitomycin C-inactivated MEF feeder cells and were passaged every 5–7 days by a brief PBS wash followed by single-cell dissociation using 3–5 min treatment with Accutase (GIBCO) and centrifugation in fibroblast medium (DMEM [Invitrogen] supplemented with 10% FBS [Hyclone], 1 mM glutamine [Invitrogen], 1% nonessential amino acids [Invitrogen], penicillin-streptomycin [Invitrogen], and 0.1 mM β-mercaptoethanol).

    Expressing:

    Article Title: Transcriptome Analysis Reveals New Insights into the Modulation of Endometrial Stromal Cell Receptive Phenotype by Embryo-Derived Signals Interleukin-1 and Human Chorionic Gonadotropin: Possible Involvement in Early Embryo Implantation
    Article Snippet: The significance of statistical differences was determined using one way analysis of variance (ANOVA) followed by the Bonferroni test post hoc , for multiple comparisons, and the Student's t-test for the comparison of two groups. .. Gene expression profile for all experiments Total RNA was isolated from primary human ESCs treated with hCG (100 ng/mL) for 24 h before being stimulated or not with IL1B (0.1 ng/mL) for additional 24 h. RNA from all treated groups was compared by micro-array with the corresponding non-treated control using Affymetrix GeneChip Human Genome. ..

    Article Title: Integrative genomics and transcriptomics analysis of human embryonic and induced pluripotent stem cells
    Article Snippet: .. We studied human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) measuring the SNPs and CNVs with Affymetrix SNP 6 microarrays and expression values with Affymetrix Exon microarrays. .. We computed the linear relationships between SNPs and expression levels of exons, transcripts and genes, and the associations between gene CNVs and gene expression levels.

    Isolation:

    Article Title: Transcriptome Analysis Reveals New Insights into the Modulation of Endometrial Stromal Cell Receptive Phenotype by Embryo-Derived Signals Interleukin-1 and Human Chorionic Gonadotropin: Possible Involvement in Early Embryo Implantation
    Article Snippet: The significance of statistical differences was determined using one way analysis of variance (ANOVA) followed by the Bonferroni test post hoc , for multiple comparisons, and the Student's t-test for the comparison of two groups. .. Gene expression profile for all experiments Total RNA was isolated from primary human ESCs treated with hCG (100 ng/mL) for 24 h before being stimulated or not with IL1B (0.1 ng/mL) for additional 24 h. RNA from all treated groups was compared by micro-array with the corresponding non-treated control using Affymetrix GeneChip Human Genome. ..

    Microarray:

    Article Title: Transcriptome Analysis Reveals New Insights into the Modulation of Endometrial Stromal Cell Receptive Phenotype by Embryo-Derived Signals Interleukin-1 and Human Chorionic Gonadotropin: Possible Involvement in Early Embryo Implantation
    Article Snippet: The significance of statistical differences was determined using one way analysis of variance (ANOVA) followed by the Bonferroni test post hoc , for multiple comparisons, and the Student's t-test for the comparison of two groups. .. Gene expression profile for all experiments Total RNA was isolated from primary human ESCs treated with hCG (100 ng/mL) for 24 h before being stimulated or not with IL1B (0.1 ng/mL) for additional 24 h. RNA from all treated groups was compared by micro-array with the corresponding non-treated control using Affymetrix GeneChip Human Genome. ..

    Cell Differentiation:

    Article Title: Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells
    Article Snippet: Due to the high complexity of the miRNAome, a genome-wide approach is mandatory for a better comprehension of the role these small non-coding RNAs have and functional studies that include all microRNAs members of families/clusters will be necessary to further understand their proper relevance in the differentiation process. .. Cell Culture and cell differentiation Human embryonic stem cells (H9-hTnnTZ-pGZ-D2) were obtained from WiCell and maintained in co-culture with irradiated primary mouse embryonic fibroblasts (MEFs) in the presence of FGF (8 ng/ml; Thermo Fisher Scientific) under conditions described by the supplier, as previously reported . .. This cell line has been genetically modified in WiCell to express both GFP and Zeocin resistance under the promoter of cardiac troponin T. All experiments were carried out in accordance with relevant guidelines and regulations.

    Co-Culture Assay:

    Article Title: Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells
    Article Snippet: Due to the high complexity of the miRNAome, a genome-wide approach is mandatory for a better comprehension of the role these small non-coding RNAs have and functional studies that include all microRNAs members of families/clusters will be necessary to further understand their proper relevance in the differentiation process. .. Cell Culture and cell differentiation Human embryonic stem cells (H9-hTnnTZ-pGZ-D2) were obtained from WiCell and maintained in co-culture with irradiated primary mouse embryonic fibroblasts (MEFs) in the presence of FGF (8 ng/ml; Thermo Fisher Scientific) under conditions described by the supplier, as previously reported . .. This cell line has been genetically modified in WiCell to express both GFP and Zeocin resistance under the promoter of cardiac troponin T. All experiments were carried out in accordance with relevant guidelines and regulations.

    Irradiation:

    Article Title: Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells
    Article Snippet: Due to the high complexity of the miRNAome, a genome-wide approach is mandatory for a better comprehension of the role these small non-coding RNAs have and functional studies that include all microRNAs members of families/clusters will be necessary to further understand their proper relevance in the differentiation process. .. Cell Culture and cell differentiation Human embryonic stem cells (H9-hTnnTZ-pGZ-D2) were obtained from WiCell and maintained in co-culture with irradiated primary mouse embryonic fibroblasts (MEFs) in the presence of FGF (8 ng/ml; Thermo Fisher Scientific) under conditions described by the supplier, as previously reported . .. This cell line has been genetically modified in WiCell to express both GFP and Zeocin resistance under the promoter of cardiac troponin T. All experiments were carried out in accordance with relevant guidelines and regulations.

    Modification:

    Article Title: Imbalanced Production of Reactive Oxygen Species and Mitochondrial Antioxidant SOD2 in Fabry Disease-Specific Human Induced Pluripotent Stem Cell-Differentiated Vascular Endothelial Cells
    Article Snippet: Furthermore, PBMCs (2 × 106 ) were reprogrammed by transfecting a mixture of plasmids containing PCXLE-hOCT3/4-shp53 (0.83 μg), PCXLE-hSK (0.83 μg), pCXLE-hUL (0.83 μg), and pCXWB-EBNA1 (0.5 μg) using the Amaxa™ Human T Cell Nucleofector™ kit and Amaxa Nucleofector II Program V-024 (Lonza). .. PBMCs were then cocultured with inactivated mouse embryonic fibroblasts as feeder cells and activated using interleukin-2 (IL-2) (175 U/ml) (PeproTech, Rocky Hill, NJ, USA) and Dynabead T-Activator CD3/CD28 (3.75 μl/ml; Thermo Fisher Scientific, Grand Island, N Y, USA) in human embryonic stem cell medium containing Dulbecco's modified Eagle's medium (DMEM)/F12 (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 0.1 mM nonessential amino acids (Thermo Fisher Scientific), 1 mM L -glutamine (Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma-Aldrich), 10 ng/ml recombinant human basic fibroblast growth factor (hbFGF) (PeproTech), and antibiotics (Thermo Fisher Scientific). .. Alkaline phosphatase (ALP)-positive hiPSC colonies were selected from day 21 to day 28 after nucleofection; undifferentiated hiPSCs were transferred to a Geltrax (Thermo Fisher Scientific)-coated culture dish and cultured in mTeSR1 medium (StemCell Technologies, Vancouver, Canada).

    Knock-Out:

    Article Title: Imbalanced Production of Reactive Oxygen Species and Mitochondrial Antioxidant SOD2 in Fabry Disease-Specific Human Induced Pluripotent Stem Cell-Differentiated Vascular Endothelial Cells
    Article Snippet: Furthermore, PBMCs (2 × 106 ) were reprogrammed by transfecting a mixture of plasmids containing PCXLE-hOCT3/4-shp53 (0.83 μg), PCXLE-hSK (0.83 μg), pCXLE-hUL (0.83 μg), and pCXWB-EBNA1 (0.5 μg) using the Amaxa™ Human T Cell Nucleofector™ kit and Amaxa Nucleofector II Program V-024 (Lonza). .. PBMCs were then cocultured with inactivated mouse embryonic fibroblasts as feeder cells and activated using interleukin-2 (IL-2) (175 U/ml) (PeproTech, Rocky Hill, NJ, USA) and Dynabead T-Activator CD3/CD28 (3.75 μl/ml; Thermo Fisher Scientific, Grand Island, N Y, USA) in human embryonic stem cell medium containing Dulbecco's modified Eagle's medium (DMEM)/F12 (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 0.1 mM nonessential amino acids (Thermo Fisher Scientific), 1 mM L -glutamine (Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma-Aldrich), 10 ng/ml recombinant human basic fibroblast growth factor (hbFGF) (PeproTech), and antibiotics (Thermo Fisher Scientific). .. Alkaline phosphatase (ALP)-positive hiPSC colonies were selected from day 21 to day 28 after nucleofection; undifferentiated hiPSCs were transferred to a Geltrax (Thermo Fisher Scientific)-coated culture dish and cultured in mTeSR1 medium (StemCell Technologies, Vancouver, Canada).

    Recombinant:

    Article Title: Imbalanced Production of Reactive Oxygen Species and Mitochondrial Antioxidant SOD2 in Fabry Disease-Specific Human Induced Pluripotent Stem Cell-Differentiated Vascular Endothelial Cells
    Article Snippet: Furthermore, PBMCs (2 × 106 ) were reprogrammed by transfecting a mixture of plasmids containing PCXLE-hOCT3/4-shp53 (0.83 μg), PCXLE-hSK (0.83 μg), pCXLE-hUL (0.83 μg), and pCXWB-EBNA1 (0.5 μg) using the Amaxa™ Human T Cell Nucleofector™ kit and Amaxa Nucleofector II Program V-024 (Lonza). .. PBMCs were then cocultured with inactivated mouse embryonic fibroblasts as feeder cells and activated using interleukin-2 (IL-2) (175 U/ml) (PeproTech, Rocky Hill, NJ, USA) and Dynabead T-Activator CD3/CD28 (3.75 μl/ml; Thermo Fisher Scientific, Grand Island, N Y, USA) in human embryonic stem cell medium containing Dulbecco's modified Eagle's medium (DMEM)/F12 (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 0.1 mM nonessential amino acids (Thermo Fisher Scientific), 1 mM L -glutamine (Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma-Aldrich), 10 ng/ml recombinant human basic fibroblast growth factor (hbFGF) (PeproTech), and antibiotics (Thermo Fisher Scientific). .. Alkaline phosphatase (ALP)-positive hiPSC colonies were selected from day 21 to day 28 after nucleofection; undifferentiated hiPSCs were transferred to a Geltrax (Thermo Fisher Scientific)-coated culture dish and cultured in mTeSR1 medium (StemCell Technologies, Vancouver, Canada).

    Flow Cytometry:

    Article Title: Overexpression of BCL2 enhances survival of human embryonic stem cells during stress and obviates the requirement for serum factors
    Article Snippet: Single-cell suspensions of hESCs or derived products were stained in staining media (PBS with 2% FCS), on ice, for 15 min. .. The BrdU analog EdU was added to healthy hESC cultures at 10 μM for 2 h. Human ESCs were then dissociated into single cells and the cell cycle status analyzed using the Click-iT EdU flow cytometry assay kit (Cat. no. A10202; Invitrogen) according to the manufacturer's protocol. .. , and 6-μm sections were prepared for immunohistochemical analysis.

    Cytometry:

    Article Title: Overexpression of BCL2 enhances survival of human embryonic stem cells during stress and obviates the requirement for serum factors
    Article Snippet: Single-cell suspensions of hESCs or derived products were stained in staining media (PBS with 2% FCS), on ice, for 15 min. .. The BrdU analog EdU was added to healthy hESC cultures at 10 μM for 2 h. Human ESCs were then dissociated into single cells and the cell cycle status analyzed using the Click-iT EdU flow cytometry assay kit (Cat. no. A10202; Invitrogen) according to the manufacturer's protocol. .. , and 6-μm sections were prepared for immunohistochemical analysis.

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  • 95
    Thermo Fisher xf ksr human embryonic stem cell hesc medium
    Xf Ksr Human Embryonic Stem Cell Hesc Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xf ksr human embryonic stem cell hesc medium/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xf ksr human embryonic stem cell hesc medium - by Bioz Stars, 2021-04
    95/100 stars
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