xcmi xbai  (New England Biolabs)


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  • 87

    Structured Review

    New England Biolabs xcmi xbai
    Xcmi Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xcmi xbai/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xcmi xbai - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: Full length rNlrp1 was amplified from LEW cDNA with Phusion polymerase (Finnzymes, Woburn, MA) and blunt-end TOPO cloned into the pCR-Blunt II-TOPO vector (Invitrogen). .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs.

    Amplification:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: Full length rNlrp1 was amplified from LEW cDNA with Phusion polymerase (Finnzymes, Woburn, MA) and blunt-end TOPO cloned into the pCR-Blunt II-TOPO vector (Invitrogen). .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs.

    Ligation:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs. .. For retroviral expression, 5′ BamHI and 3′ NotI sites were introduced into full length CDF53-LEW and LEW rNlrp1 amplified from CDF53-LEW-pIREShygV3 and LEW-pIREShygV3 using Phusion polymerase (New England Biolabs).

    Introduce:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs. .. QuikChange (Agilent Technologies) was used to introduce 4 and 7 nucleotide changes into CDF53-LEW-pFB-NEO to create CDF53(EQ)-LEW-pFB-NEO and CDF53(QVEQ)-LEW-pFB-NEO.

    Construct:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs. .. For retroviral expression, 5′ BamHI and 3′ NotI sites were introduced into full length CDF53-LEW and LEW rNlrp1 amplified from CDF53-LEW-pIREShygV3 and LEW-pIREShygV3 using Phusion polymerase (New England Biolabs).

    Expressing:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: NheI/EcoRV digested fragments were ligated into the pIREShyg3 mammalian expression vector (Clontech, Mountain View, CA). .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs.

    Polymerase Chain Reaction:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: Full length rNlrp1 was amplified from LEW cDNA with Phusion polymerase (Finnzymes, Woburn, MA) and blunt-end TOPO cloned into the pCR-Blunt II-TOPO vector (Invitrogen). .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs.

    Plasmid Preparation:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: NheI/EcoRV digested fragments were ligated into the pIREShyg3 mammalian expression vector (Clontech, Mountain View, CA). .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs.

    Gel Purification:

    Article Title: Anthrax Lethal Factor Cleavage of Nlrp1 Is Required for Activation of the Inflammasome
    Article Snippet: .. CDF53-LEW- and LEW53-CDF chimeric constructs were made by digestion of CDF-pIREShygV3 and LEW-pIREShygV3 with XcmI/XbaI (New England Biolabs, Ipswich, MA), gel purification of fragments, followed by ligation to yield the appropriate fused constructs. .. For retroviral expression, 5′ BamHI and 3′ NotI sites were introduced into full length CDF53-LEW and LEW rNlrp1 amplified from CDF53-LEW-pIREShygV3 and LEW-pIREShygV3 using Phusion polymerase (New England Biolabs).

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  • News
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  • Team
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  • Bioz Stars
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  • 87
    New England Biolabs xcmi xbai
    Xcmi Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xcmi xbai/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xcmi xbai - by Bioz Stars, 2020-04
    87/100 stars
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