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The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J ) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers <t>myf5</t> and myoD is perturbed in fgfr4 CRISPR embryos. (K, L) <t>Xbra</t> expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; ** p
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1) Product Images from "Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus"

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2018.01705

The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J ) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers myf5 and myoD is perturbed in fgfr4 CRISPR embryos. (K, L) Xbra expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; ** p
Figure Legend Snippet: The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J ) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers myf5 and myoD is perturbed in fgfr4 CRISPR embryos. (K, L) Xbra expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; ** p

Techniques Used: CRISPR, Expressing, Marker

Related Articles

Incubation:

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

In Vitro:

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: D-loops were defined as outflow tracts directed to the right, and L-loops to the left. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Embryos were collected at the desired stages, fixed in MEMFA for 1–2 h at room temperature (RT) and dehydrated in 100% ethanol.

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: D-loops were defined as outflow tracts directed to the right, and L-loops to the left. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Embryos were collected at the desired stages, fixed in MEMFA for 1–2 h at room temperature (RT) and dehydrated in 100% ethanol.

Labeling:

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs. .. Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.

In Situ Hybridization:

Article Title: Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus
Article Snippet: Paragraph title: In situ Hybridization ... Digoxigenin-labeled antisense probes for pitx2 (TNeu083k20), coco (TEgg007d24), xnr1 (TGas124h10), gdf3 (Tgas137g21), gsc (TNeu077f20), xnr3 (Tgas011k18), foxj1 (Tneu058M03), vent2 (BG885317), myf5 (TGas127b01), xbra (TNeu024F07), fgfr4 (Dharmacon, Clone ID: 7521919) were in vitro transcribed using T7 High Yield RNA Synthesis Kit (E2040S) from New England Biolabs.