Structured Review

TaKaRa xbai
Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xbai/product/TaKaRa
Average 90 stars, based on 26 article reviews
Price from $9.99 to $1999.99
xbai - by Bioz Stars, 2020-02
90/100 stars

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Related Articles

Clone Assay:

Article Title: Pituitary Somatolactotropes Evade an Oncogenic Response to Ras
Article Snippet: Paragraph title: Doxycycline-inducible construct cloning ... The plasmid was cut with HindIII and XbaI to remove the Ras insert. pTRE-Tight vector was purchased from CLONTECH Laboratories Inc. and cut with HindIII, XbaI, and then calf intestinal alkaline phosphatase (Fisher Scientific).

Article Title: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep
Article Snippet: .. The resulting product was then cloned into the XbaI and BamHI sites of the plasmid pEX18-Gm (Takara) to yield pEX18-ppa, which was then conjugated into strain SWP-3 from E. coli DH5α with the help of the plasmid pRK2013. ..

Amplification:

Article Title: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep
Article Snippet: The resulting product was then cloned into the XbaI and BamHI sites of the plasmid pEX18-Gm (Takara) to yield pEX18-ppa, which was then conjugated into strain SWP-3 from E. coli DH5α with the help of the plasmid pRK2013. .. For the complementation of the disrupted ppa gene, the ppa gene was amplified with the primer pair PPA1-F/PPA1-R (Additional file : Table S1) and ligated into the corresponding sites of the broad-host-range plasmid pBBR1-MCS2 [ ], yielding pBBR1-ppa.

Mutagenesis:

Article Title: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep
Article Snippet: The resulting product was then cloned into the XbaI and BamHI sites of the plasmid pEX18-Gm (Takara) to yield pEX18-ppa, which was then conjugated into strain SWP-3 from E. coli DH5α with the help of the plasmid pRK2013. .. The mutant SWP-3 M with the disrupted ppa gene was checked by PCR.

Construct:

Article Title: Pituitary Somatolactotropes Evade an Oncogenic Response to Ras
Article Snippet: Paragraph title: Doxycycline-inducible construct cloning ... The plasmid was cut with HindIII and XbaI to remove the Ras insert. pTRE-Tight vector was purchased from CLONTECH Laboratories Inc. and cut with HindIII, XbaI, and then calf intestinal alkaline phosphatase (Fisher Scientific).

Article Title: αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
Article Snippet: Paragraph title: Construction of zebrafish α-catenin-GFP constructs ... We then digested the PCR product with XhoI and XbaI, and the pEGFP parental vector with SalI and XbaI (Clontech), after which we ligated the product, resulting in ZFα-catenin-GFP x pEGFP.

Generated:

Article Title: αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
Article Snippet: We generated a GFP-tagged version by generating a PCR fragment containing XhoI/XbaI restriction sites at the terminal ends using the following primers: forward 5′- GACACTCGAGCCATGACGAGCATTAACACTGCTAACATC-3′ and reverse: 5′- GACATCTAGACGGAGGAGAGTAGATTAGACTTA-3′. .. We then digested the PCR product with XhoI and XbaI, and the pEGFP parental vector with SalI and XbaI (Clontech), after which we ligated the product, resulting in ZFα-catenin-GFP x pEGFP.

Article Title: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep
Article Snippet: Genetic disruption and complementation To disrupt the ppa gene by gene targeting, two DNA fragments (approximately 730 bp flanking each end of ppa ) were generated by PCR using the primer pairs PP1-F/PP1-R and PP2-F/PP2-R (Additional file : Table S1). .. The resulting product was then cloned into the XbaI and BamHI sites of the plasmid pEX18-Gm (Takara) to yield pEX18-ppa, which was then conjugated into strain SWP-3 from E. coli DH5α with the help of the plasmid pRK2013.

Polymerase Chain Reaction:

Article Title: αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
Article Snippet: .. We then digested the PCR product with XhoI and XbaI, and the pEGFP parental vector with SalI and XbaI (Clontech), after which we ligated the product, resulting in ZFα-catenin-GFP x pEGFP. .. We then subcloned α-catenin-GFP into pCS2 vector for mRNA transcription.

Article Title: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep
Article Snippet: Subsequently, the two fragments were joined using the primer pair PP1-F/PP2-R (Additional file : Table S1) by overlap extension PCR. .. The resulting product was then cloned into the XbaI and BamHI sites of the plasmid pEX18-Gm (Takara) to yield pEX18-ppa, which was then conjugated into strain SWP-3 from E. coli DH5α with the help of the plasmid pRK2013.

Plasmid Preparation:

Article Title: Pituitary Somatolactotropes Evade an Oncogenic Response to Ras
Article Snippet: .. The plasmid was cut with HindIII and XbaI to remove the Ras insert. pTRE-Tight vector was purchased from CLONTECH Laboratories Inc. and cut with HindIII, XbaI, and then calf intestinal alkaline phosphatase (Fisher Scientific). .. BS/IRES-M2 clone 13 was established by co-transfecting GH4T2 cells with BS/IRES-M2 plasmid (a kind gift from Dr. Stefania Lamartina, Istituto Di Richerche Di Biologia Molecolare, Rome, Italy) and empty pEGFP-C3 vector (Invitrogen; 10:1 ratio) by electroporation.

Article Title: αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
Article Snippet: .. We then digested the PCR product with XhoI and XbaI, and the pEGFP parental vector with SalI and XbaI (Clontech), after which we ligated the product, resulting in ZFα-catenin-GFP x pEGFP. .. We then subcloned α-catenin-GFP into pCS2 vector for mRNA transcription.

Article Title: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep
Article Snippet: .. The resulting product was then cloned into the XbaI and BamHI sites of the plasmid pEX18-Gm (Takara) to yield pEX18-ppa, which was then conjugated into strain SWP-3 from E. coli DH5α with the help of the plasmid pRK2013. ..

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    TaKaRa xbai gfp sharpin
    The UBL domain of <t>SHARPIN</t> mediates binding to integrin. (A) Schematic representation of SHARPIN with its functional domains and the SHARPIN fragments used in this study. (B) Pull-down experiments to determine the interaction between <t>GFP-SHARPIN</t> (full-length or fragments) and peptides corresponding to the cytoplasmic domain of ITGAL and ITGB2. (C) Far-Western analysis of GST-SHARPIN (full-length or fragments) binding to full-length ITGAL-ITGB2 or ITGAL-ITGB2 lacking both cytoplasmic tails. Loading controls for GST-SHARPIN (full-length or fragments) and both ITGAL-ITGB2s are shown. (D) Fluorescence polarization-based titration of GST-SHARPIN (full-length or fragments) binding to an integrin peptide corresponding to the conserved domain within the cytoplasmic tail of ITGA2. Average normalized binding curves are shown (mean ± s.e.m. ***: p
    Xbai Gfp Sharpin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai gfp sharpin/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai gfp sharpin - by Bioz Stars, 2020-02
    86/100 stars
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    xbai  (TaKaRa)
    90
    TaKaRa xbai
    Genetic relatedness of E. coli isolates with class 1 integrons indicated by <t>XbaI</t> -digested chromosomal <t>DNA</t>
    Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai/product/TaKaRa
    Average 90 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    xbai - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    99
    TaKaRa restriction enzyme xbai
    Gel image of PFGE result. Genomic <t>DNA</t> was digested using <t>XbaI</t> enzyme and subjected to pulsed-field gel electrophoresis.
    Restriction Enzyme Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme xbai/product/TaKaRa
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme xbai - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    The UBL domain of SHARPIN mediates binding to integrin. (A) Schematic representation of SHARPIN with its functional domains and the SHARPIN fragments used in this study. (B) Pull-down experiments to determine the interaction between GFP-SHARPIN (full-length or fragments) and peptides corresponding to the cytoplasmic domain of ITGAL and ITGB2. (C) Far-Western analysis of GST-SHARPIN (full-length or fragments) binding to full-length ITGAL-ITGB2 or ITGAL-ITGB2 lacking both cytoplasmic tails. Loading controls for GST-SHARPIN (full-length or fragments) and both ITGAL-ITGB2s are shown. (D) Fluorescence polarization-based titration of GST-SHARPIN (full-length or fragments) binding to an integrin peptide corresponding to the conserved domain within the cytoplasmic tail of ITGA2. Average normalized binding curves are shown (mean ± s.e.m. ***: p

    Journal: PLoS ONE

    Article Title: Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling

    doi: 10.1371/journal.pone.0143423

    Figure Lengend Snippet: The UBL domain of SHARPIN mediates binding to integrin. (A) Schematic representation of SHARPIN with its functional domains and the SHARPIN fragments used in this study. (B) Pull-down experiments to determine the interaction between GFP-SHARPIN (full-length or fragments) and peptides corresponding to the cytoplasmic domain of ITGAL and ITGB2. (C) Far-Western analysis of GST-SHARPIN (full-length or fragments) binding to full-length ITGAL-ITGB2 or ITGAL-ITGB2 lacking both cytoplasmic tails. Loading controls for GST-SHARPIN (full-length or fragments) and both ITGAL-ITGB2s are shown. (D) Fluorescence polarization-based titration of GST-SHARPIN (full-length or fragments) binding to an integrin peptide corresponding to the conserved domain within the cytoplasmic tail of ITGA2. Average normalized binding curves are shown (mean ± s.e.m. ***: p

    Article Snippet: For construction of the GFP- and GST-SHARPIN fragments, the corresponding DNA fragments were amplified from siRNA1-insensitive GFP-Sharpin using specific primers that introduce XhoI and XbaI (GFP-SHARPIN) or EcoRI and BamHI sites (GST-SHARPIN), followed by cloning into pEGFPC1 (Clontech) and pGEX4T-1 vectors, respectively.

    Techniques: Binding Assay, Functional Assay, Western Blot, Fluorescence, Titration

    Integrin and RNF31 binding to SHARPIN are mutually exclusive. (A) Pull-down assay showing that the presence of a peptide corresponding to the cytoplasmic domain of ITGAL prevents interaction between GST-SHARPIN and RNF31. (B) Co-immunoprecipitation of endogenous SHARPIN, RNF31 and ITGAL from Jurkat cells (a GFP antibody was used as negative control). (C) Pull-down of GFP-SHARPIN from HEK293 cells demonstrated that cells in suspension show decreased RNF31-SHARPIN interaction compared to adherent cells (n = 4). RNF31 binding was normalized to total RNF31 levels and the amount of pulled-down GFP-SHARPIN. (D) TNF-induced NF-κB promoter activity of PC3 cells, adherent to either 5 μg/ml fibronectin or poly-L-lysin (a substratum for integrin-independent cell adhesion), was measured using a luciferase reporter assay (n = 2 with 5 replicates each). (E) TNF-induced NF-κB promoter activity of PC3 cells or WT MEFs, incubated with a membrane-permeable ITGA1-tail peptide (α1-TAT) or a scrambled peptide (ScrTAT), was measured using a luciferase reporter assay (n = 2 with 6–9 replicates, and n = 3 with 3–5 replicates, respectively). All numerical data are mean ± s.e.m. ***: p

    Journal: PLoS ONE

    Article Title: Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling

    doi: 10.1371/journal.pone.0143423

    Figure Lengend Snippet: Integrin and RNF31 binding to SHARPIN are mutually exclusive. (A) Pull-down assay showing that the presence of a peptide corresponding to the cytoplasmic domain of ITGAL prevents interaction between GST-SHARPIN and RNF31. (B) Co-immunoprecipitation of endogenous SHARPIN, RNF31 and ITGAL from Jurkat cells (a GFP antibody was used as negative control). (C) Pull-down of GFP-SHARPIN from HEK293 cells demonstrated that cells in suspension show decreased RNF31-SHARPIN interaction compared to adherent cells (n = 4). RNF31 binding was normalized to total RNF31 levels and the amount of pulled-down GFP-SHARPIN. (D) TNF-induced NF-κB promoter activity of PC3 cells, adherent to either 5 μg/ml fibronectin or poly-L-lysin (a substratum for integrin-independent cell adhesion), was measured using a luciferase reporter assay (n = 2 with 5 replicates each). (E) TNF-induced NF-κB promoter activity of PC3 cells or WT MEFs, incubated with a membrane-permeable ITGA1-tail peptide (α1-TAT) or a scrambled peptide (ScrTAT), was measured using a luciferase reporter assay (n = 2 with 6–9 replicates, and n = 3 with 3–5 replicates, respectively). All numerical data are mean ± s.e.m. ***: p

    Article Snippet: For construction of the GFP- and GST-SHARPIN fragments, the corresponding DNA fragments were amplified from siRNA1-insensitive GFP-Sharpin using specific primers that introduce XhoI and XbaI (GFP-SHARPIN) or EcoRI and BamHI sites (GST-SHARPIN), followed by cloning into pEGFPC1 (Clontech) and pGEX4T-1 vectors, respectively.

    Techniques: Binding Assay, Pull Down Assay, Immunoprecipitation, Negative Control, Activity Assay, Luciferase, Reporter Assay, Incubation

    Residues V267 and L276 are essential for SHARPIN-mediated integrin inhibition. (A) FACS analysis of CHO cells overexpressing GFP alone, or WT or mutant GFP-SHARPIN, together with RFP-TALIN head. The Integrin Activation Index was calculated by dividing active cell-surface integrin levels (FN7-10 binding minus FN7-10 binding in the presence of EDTA) by total cell-surface integrin levels (Mb1.2 staining minus secondary antibody alone) (n = 3). (B) Quantification of migration speed of cpdm MEFs overexpressing GFP alone or GFP-SHARPIN WT on 50 μg/ml collagen (n = 78 and 83 cells, respectively). (C,D) Quantification of migration speed (n = 27–125 cells) (C), and representative cell tracks (D) of cpdm MEFs overexpressing WT or mutant GFP-SHARPIN on 50 μg/ml collagen. All numerical data are mean ± s.e.m. ***: p

    Journal: PLoS ONE

    Article Title: Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling

    doi: 10.1371/journal.pone.0143423

    Figure Lengend Snippet: Residues V267 and L276 are essential for SHARPIN-mediated integrin inhibition. (A) FACS analysis of CHO cells overexpressing GFP alone, or WT or mutant GFP-SHARPIN, together with RFP-TALIN head. The Integrin Activation Index was calculated by dividing active cell-surface integrin levels (FN7-10 binding minus FN7-10 binding in the presence of EDTA) by total cell-surface integrin levels (Mb1.2 staining minus secondary antibody alone) (n = 3). (B) Quantification of migration speed of cpdm MEFs overexpressing GFP alone or GFP-SHARPIN WT on 50 μg/ml collagen (n = 78 and 83 cells, respectively). (C,D) Quantification of migration speed (n = 27–125 cells) (C), and representative cell tracks (D) of cpdm MEFs overexpressing WT or mutant GFP-SHARPIN on 50 μg/ml collagen. All numerical data are mean ± s.e.m. ***: p

    Article Snippet: For construction of the GFP- and GST-SHARPIN fragments, the corresponding DNA fragments were amplified from siRNA1-insensitive GFP-Sharpin using specific primers that introduce XhoI and XbaI (GFP-SHARPIN) or EcoRI and BamHI sites (GST-SHARPIN), followed by cloning into pEGFPC1 (Clontech) and pGEX4T-1 vectors, respectively.

    Techniques: Inhibition, FACS, Mutagenesis, Activation Assay, Binding Assay, Staining, Migration

    Fine mapping of the integrin binding site in SHARPIN. (A) Interaction of different GST-SHARPIN (WT and all point mutants) with full-length ITGAL-ITGB2 was determined using Far-Western assays. GST-SHARPIN 1-180 was used as negative control. (B) HEK293 cancer cells, overexpressing WT or mutant GFP-SHARPIN in combination with ITGA5–mCherry, subjected to FRET analysis by FLIM. Fluorescence lifetimes, mapping spatial FRET in cells, are depicted using a pseudo-color scale (blue, normal lifetime; red, FRET (reduced lifetime)). Scale bars: 10 μm. (C) Quantification of FRET efficiency for all mutants (n ≥ 12 cells). All numerical data are mean ± s.e.m. ***: p

    Journal: PLoS ONE

    Article Title: Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling

    doi: 10.1371/journal.pone.0143423

    Figure Lengend Snippet: Fine mapping of the integrin binding site in SHARPIN. (A) Interaction of different GST-SHARPIN (WT and all point mutants) with full-length ITGAL-ITGB2 was determined using Far-Western assays. GST-SHARPIN 1-180 was used as negative control. (B) HEK293 cancer cells, overexpressing WT or mutant GFP-SHARPIN in combination with ITGA5–mCherry, subjected to FRET analysis by FLIM. Fluorescence lifetimes, mapping spatial FRET in cells, are depicted using a pseudo-color scale (blue, normal lifetime; red, FRET (reduced lifetime)). Scale bars: 10 μm. (C) Quantification of FRET efficiency for all mutants (n ≥ 12 cells). All numerical data are mean ± s.e.m. ***: p

    Article Snippet: For construction of the GFP- and GST-SHARPIN fragments, the corresponding DNA fragments were amplified from siRNA1-insensitive GFP-Sharpin using specific primers that introduce XhoI and XbaI (GFP-SHARPIN) or EcoRI and BamHI sites (GST-SHARPIN), followed by cloning into pEGFPC1 (Clontech) and pGEX4T-1 vectors, respectively.

    Techniques: Binding Assay, Western Blot, Negative Control, Mutagenesis, Fluorescence

    Fine mapping of the RNF31 binding site in SHARPIN. (A) Western blot analysis of SHARPIN and β-tubulin levels in control- or SHARPIN-silenced PC3 cells. (B) TNF-induced NF-κB promoter activity of SHARPIN- or control-silenced PC3 cells was measured using a luciferase reporter assay. (n = 3 with 5 replicates each). (C) TNF-induced NF-κB promoter activity of SHARPIN-silenced PC3 cells, expressing GFP alone, WT or mutant GFP-SHARPIN (n = 6–15 measurements from 2–3 experiments). (D,E) Interaction between RNF31 and WT or mutant GST-SHARPIN was determined using an ELISA-based binding assay (n = 3) (D) or Far-Western analysis (E). All numerical data are mean ± s.e.m. ***: p

    Journal: PLoS ONE

    Article Title: Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling

    doi: 10.1371/journal.pone.0143423

    Figure Lengend Snippet: Fine mapping of the RNF31 binding site in SHARPIN. (A) Western blot analysis of SHARPIN and β-tubulin levels in control- or SHARPIN-silenced PC3 cells. (B) TNF-induced NF-κB promoter activity of SHARPIN- or control-silenced PC3 cells was measured using a luciferase reporter assay. (n = 3 with 5 replicates each). (C) TNF-induced NF-κB promoter activity of SHARPIN-silenced PC3 cells, expressing GFP alone, WT or mutant GFP-SHARPIN (n = 6–15 measurements from 2–3 experiments). (D,E) Interaction between RNF31 and WT or mutant GST-SHARPIN was determined using an ELISA-based binding assay (n = 3) (D) or Far-Western analysis (E). All numerical data are mean ± s.e.m. ***: p

    Article Snippet: For construction of the GFP- and GST-SHARPIN fragments, the corresponding DNA fragments were amplified from siRNA1-insensitive GFP-Sharpin using specific primers that introduce XhoI and XbaI (GFP-SHARPIN) or EcoRI and BamHI sites (GST-SHARPIN), followed by cloning into pEGFPC1 (Clontech) and pGEX4T-1 vectors, respectively.

    Techniques: Binding Assay, Western Blot, Activity Assay, Luciferase, Reporter Assay, Expressing, Mutagenesis, Enzyme-linked Immunosorbent Assay

    Genetic relatedness of E. coli isolates with class 1 integrons indicated by XbaI -digested chromosomal DNA

    Journal: BMC Veterinary Research

    Article Title: Interrelationship between tetracycline resistance determinants, phylogenetic group affiliation and carriage of class 1 integrons in commensal Escherichia coli isolates from cattle farms

    doi: 10.1186/s12917-018-1661-3

    Figure Lengend Snippet: Genetic relatedness of E. coli isolates with class 1 integrons indicated by XbaI -digested chromosomal DNA

    Article Snippet: Briefly, following 18 to 20 h growth on TSA at 37 °C, genomic DNA was digested with 50 U XbaI (TaKaRa, Japan) for 2 h at 37 °C, then the DNA fragments were subsequently separated on a 1.0% SeaKem Gold agarose gel (Lonza, USA) in 0.5× Tris-borate-EDTA (TBE) buffer using a CHEFMapper gel apparatus (Bio-Rad Laboratories, California, USA).

    Techniques:

    Dendrograms generated by Bionumerics software showing the cluster analysis of XbaI-PFGE patterns of CTX-M (A)- and CMY-2 (B)-producing E. coli strains isolated from stray dogs. Similarity analysis was performed by using the Dice coefficient, and clustering

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Molecular Characterization of Extended-Spectrum-?-Lactamase-Producing and Plasmid-Mediated AmpC ?-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea

    doi: 10.1128/AAC.05598-11

    Figure Lengend Snippet: Dendrograms generated by Bionumerics software showing the cluster analysis of XbaI-PFGE patterns of CTX-M (A)- and CMY-2 (B)-producing E. coli strains isolated from stray dogs. Similarity analysis was performed by using the Dice coefficient, and clustering

    Article Snippet: Pulsed-field gel electrophoresis (PFGE) of XbaI (Takara Bio Inc., Shiga, Japan)-digested genomic DNA was carried out according to the CDC pulseNet standardized procedure , using a Chef Mapper apparatus (Bio-Rad Laboratories, Hercules, CA).

    Techniques: Generated, Software, Isolation

    Gel image of PFGE result. Genomic DNA was digested using XbaI enzyme and subjected to pulsed-field gel electrophoresis.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Clinical and Molecular Characteristics of Emerging Hypervirulent Klebsiella pneumoniae Bloodstream Infections in Mainland China

    doi: 10.1128/AAC.02523-14

    Figure Lengend Snippet: Gel image of PFGE result. Genomic DNA was digested using XbaI enzyme and subjected to pulsed-field gel electrophoresis.

    Article Snippet: Whole-cell genomic DNA representing each isolate was digested with the restriction enzyme XbaI (TaKaRa Biotechnology, Dalian, China) and separated by electrophoresis through 1% pulsed-field certified agarose (Bio-Rad, Richmond, CA, USA) by using a CHEF-Mapper (Bio-Rad).

    Techniques: Pulsed-Field Gel, Electrophoresis