xbai  (TaKaRa)


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    • 86
    Name:
    Xba I
    Description:
    T | CTAG AA GATC | T
    Catalog Number:
    1093b
    Price:
    None
    Category:
    XbaI Restriction enzymes Cloning
    Size:
    15 000 Units
    		Buy from Supplier

    Structured Review

    TaKaRa xbai
    The molecular epidemiology of DEC and <t>NTS</t> strains isolated from subjects over five years of age. The PFGE pattern of DEC was discrete, but the pulsotype pattern of NTS strains was closely related to the serotype of NTS. The dendrogram was produced using the Dice coefficient and the UPGMA with a position tolerance of 1.0%; PFGE using <t>XbaI</t> endonuclease
    T | CTAG AA GATC | T
    https://www.bioz.com/result/xbai/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Antibiotic resistance and molecular characterization of diarrheagenic Escherichia coli and non-typhoidal Salmonella strains isolated from infections in Southwest China"

    Article Title: Antibiotic resistance and molecular characterization of diarrheagenic Escherichia coli and non-typhoidal Salmonella strains isolated from infections in Southwest China

    Journal: Infectious Diseases of Poverty

    doi: 10.1186/s40249-018-0427-2

    The molecular epidemiology of DEC and NTS strains isolated from subjects over five years of age. The PFGE pattern of DEC was discrete, but the pulsotype pattern of NTS strains was closely related to the serotype of NTS. The dendrogram was produced using the Dice coefficient and the UPGMA with a position tolerance of 1.0%; PFGE using XbaI endonuclease
    Figure Legend Snippet: The molecular epidemiology of DEC and NTS strains isolated from subjects over five years of age. The PFGE pattern of DEC was discrete, but the pulsotype pattern of NTS strains was closely related to the serotype of NTS. The dendrogram was produced using the Dice coefficient and the UPGMA with a position tolerance of 1.0%; PFGE using XbaI endonuclease

    Techniques Used: Isolation, Produced

    The molecular epidemiology of DEC and NTS strains isolated from subjects under five years of age. The PFGE pattern of DEC was discrete, but the pulsotype pattern of NTS strains were closely related to the serotype of NTS. The dendrogram was produced using the Dice coefficient and the UPGMA with a position tolerance of 1.0%; PFGE using XbaI endonuclease
    Figure Legend Snippet: The molecular epidemiology of DEC and NTS strains isolated from subjects under five years of age. The PFGE pattern of DEC was discrete, but the pulsotype pattern of NTS strains were closely related to the serotype of NTS. The dendrogram was produced using the Dice coefficient and the UPGMA with a position tolerance of 1.0%; PFGE using XbaI endonuclease

    Techniques Used: Isolation, Produced

    2) Product Images from "Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine"

    Article Title: Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.03696-13

    Genotyping and phylogenetic analysis of E. coli strains. A dendrogram obtained by PFGE of XbaI-digested DNA from 73 E. coli O139 and O149 strains is shown on the left side. Information about each strain, the results of the PCR screening for genes encoding
    Figure Legend Snippet: Genotyping and phylogenetic analysis of E. coli strains. A dendrogram obtained by PFGE of XbaI-digested DNA from 73 E. coli O139 and O149 strains is shown on the left side. Information about each strain, the results of the PCR screening for genes encoding

    Techniques Used: Polymerase Chain Reaction

    Related Articles

    Marker:

    Article Title: Construction of a fusion expression plasmid containing the G250 gene and human granulocyte-macrophage colony stimulating factor and its significance in renal cell carcinoma
    Article Snippet: E. coli Top 10, vector pVAX1 , recombinant plasmid pORF-hGM-CSF and pcDNA3.0-G250 were obtained from the Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou, China. .. Ex-Taq DNA polymerase, T4 DNA ligase, restriction enzymes Xba I, Hin dIII and Kpn I, 1 kb DNA marker, 100 bp DNA marker, plasmid mini and gel extraction kits were from Takara Bio Inc., Japan. .. The target gene sequencing analysis was from Shanghai Sangon Biological Engineering Technology and Services Company, China.

    Plasmid Preparation:

    Article Title: Construction of a fusion expression plasmid containing the G250 gene and human granulocyte-macrophage colony stimulating factor and its significance in renal cell carcinoma
    Article Snippet: E. coli Top 10, vector pVAX1 , recombinant plasmid pORF-hGM-CSF and pcDNA3.0-G250 were obtained from the Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou, China. .. Ex-Taq DNA polymerase, T4 DNA ligase, restriction enzymes Xba I, Hin dIII and Kpn I, 1 kb DNA marker, 100 bp DNA marker, plasmid mini and gel extraction kits were from Takara Bio Inc., Japan. .. The target gene sequencing analysis was from Shanghai Sangon Biological Engineering Technology and Services Company, China.

    Article Title: Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells
    Article Snippet: The expression vector pcDNA3.1+ was from our department. .. EcoR V, Xba I, BamH I, cloning vector pUCm-T and T4 DNA ligase were purchased from Takara; MTT, DEPC from Sigma. .. TRIZOL, M-MuLV RT enzyme, Taq DNA polymerase, DMEM, Lipofectamine and G418 were products of Gibco BRL, and Primer from Sangon, Shanghai.

    Gel Extraction:

    Article Title: Construction of a fusion expression plasmid containing the G250 gene and human granulocyte-macrophage colony stimulating factor and its significance in renal cell carcinoma
    Article Snippet: E. coli Top 10, vector pVAX1 , recombinant plasmid pORF-hGM-CSF and pcDNA3.0-G250 were obtained from the Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou, China. .. Ex-Taq DNA polymerase, T4 DNA ligase, restriction enzymes Xba I, Hin dIII and Kpn I, 1 kb DNA marker, 100 bp DNA marker, plasmid mini and gel extraction kits were from Takara Bio Inc., Japan. .. The target gene sequencing analysis was from Shanghai Sangon Biological Engineering Technology and Services Company, China.

    Clone Assay:

    Article Title: Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells
    Article Snippet: The expression vector pcDNA3.1+ was from our department. .. EcoR V, Xba I, BamH I, cloning vector pUCm-T and T4 DNA ligase were purchased from Takara; MTT, DEPC from Sigma. .. TRIZOL, M-MuLV RT enzyme, Taq DNA polymerase, DMEM, Lipofectamine and G418 were products of Gibco BRL, and Primer from Sangon, Shanghai.

    MTT Assay:

    Article Title: Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells
    Article Snippet: The expression vector pcDNA3.1+ was from our department. .. EcoR V, Xba I, BamH I, cloning vector pUCm-T and T4 DNA ligase were purchased from Takara; MTT, DEPC from Sigma. .. TRIZOL, M-MuLV RT enzyme, Taq DNA polymerase, DMEM, Lipofectamine and G418 were products of Gibco BRL, and Primer from Sangon, Shanghai.

    Pulsed-Field Gel:

    Article Title: Distribution of β-Lactamase Genes and Genetic Context of blaKPC-2 in Clinical Carbapenemase-Producing Klebsiella pneumoniae Isolates
    Article Snippet: The PCR products were sequenced and compared with the reference sequences of K. pneumoniae strain ATCC13883 (NZ_JOOW00000000.1). .. Molecular Typing All CRKP isolates were classified by Xba I (Takara, Dalian, China)-digested pulsed-field gel electrophoresis (PFGE) using a CHEF Mapper System (Bio-Rad) as previously described. .. A phylogenetic dendrogram of PFGE profiles was constructed using UPGMA clustering under appropriate tolerance (2%) settings.

    Article Title: Spread of the florfenicol resistance floR gene among clinical Klebsiella pneumoniae isolates in China
    Article Snippet: E. coli ATCC 25922 was used as a quality control strain. .. Pulsed-field gel electrophoresis (PFGE) To assess the epidemiology of clinical isolates with floR genes, genomic DNA from K. pneumoniae isolates harboring floR genes was prepared for PFGE typing and digested with 40 U of Xba I (Takara, Dalian, China). ..

    Article Title: Molecular Characterization of Extended-Spectrum-?-Lactamase-Producing and Plasmid-Mediated AmpC ?-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea
    Article Snippet: Allelic profile and sequence type (ST) determinations were done according to the E. coli MLST website ( ) scheme. .. Pulsed-field gel electrophoresis (PFGE) of XbaI (Takara Bio Inc., Shiga, Japan)-digested genomic DNA was carried out according to the CDC pulseNet standardized procedure , using a Chef Mapper apparatus (Bio-Rad Laboratories, Hercules, CA). .. The similarities of the restriction fragment length polymorphisms were analyzed using Bionumerics software, version 4.0 (Applied Maths, Sint-Martens-Latem, Belgium), to produce a dendrogram.

    Electrophoresis:

    Article Title: Characterization of Salmonella enterica Isolates from Diseased Poultry in Northern China between 2014 and 2018
    Article Snippet: .. Plugs were lysed at 54 °C for 2 h, and the obtained DNA was digested with 50 U of Xba I (TaKaRa, Dalian, China) at 37 °C for 4 h. The restriction fragments were separated in a 0.5 × Tris-borate-EDTA buffer at 14 °C for 18 h using a Chef Mapper electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA) with switch times of 2.16 s and 63.8 s. The gels were stained with GoldenView and visualized under UV transillumination. .. Salmonella Braenderup H9812 was used as the standard molecular marker.

    Staining:

    Article Title: Characterization of Salmonella enterica Isolates from Diseased Poultry in Northern China between 2014 and 2018
    Article Snippet: .. Plugs were lysed at 54 °C for 2 h, and the obtained DNA was digested with 50 U of Xba I (TaKaRa, Dalian, China) at 37 °C for 4 h. The restriction fragments were separated in a 0.5 × Tris-borate-EDTA buffer at 14 °C for 18 h using a Chef Mapper electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA) with switch times of 2.16 s and 63.8 s. The gels were stained with GoldenView and visualized under UV transillumination. .. Salmonella Braenderup H9812 was used as the standard molecular marker.

    Agarose Gel Electrophoresis:

    Article Title: Interrelationship between tetracycline resistance determinants, phylogenetic group affiliation and carriage of class 1 integrons in commensal Escherichia coli isolates from cattle farms
    Article Snippet: Clonal relationships among integron positive strains Determination of the genetic relationship between the integron positive isolates was accomplished by pulsed-field gel electrophoresis (PFGE) analysis according to the protocols and criteria previously established by the Centers for Disease Control and Prevention (CDC) using XbaI as the restriction enzyme. .. Briefly, following 18 to 20 h growth on TSA at 37 °C, genomic DNA was digested with 50 U XbaI (TaKaRa, Japan) for 2 h at 37 °C, then the DNA fragments were subsequently separated on a 1.0% SeaKem Gold agarose gel (Lonza, USA) in 0.5× Tris-borate-EDTA (TBE) buffer using a CHEFMapper gel apparatus (Bio-Rad Laboratories, California, USA). .. The conditions for electrophoresis were as follows: pulse time, 2–30s at 14 °C; run time, 18 h; voltage, 6 V/cm.

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    • restriction enzyme xbai  (TaKaRa)
    98
    TaKaRa restriction enzyme xbai
    Gel image of PFGE result. Genomic <t>DNA</t> was digested using <t>XbaI</t> enzyme and subjected to pulsed-field gel electrophoresis.
    Restriction Enzyme Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme xbai/product/TaKaRa
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme xbai - by Bioz Stars, 2021-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Gel image of PFGE result. Genomic DNA was digested using XbaI enzyme and subjected to pulsed-field gel electrophoresis.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Clinical and Molecular Characteristics of Emerging Hypervirulent Klebsiella pneumoniae Bloodstream Infections in Mainland China

    doi: 10.1128/AAC.02523-14

    Figure Lengend Snippet: Gel image of PFGE result. Genomic DNA was digested using XbaI enzyme and subjected to pulsed-field gel electrophoresis.

    Article Snippet: Whole-cell genomic DNA representing each isolate was digested with the restriction enzyme XbaI (TaKaRa Biotechnology, Dalian, China) and separated by electrophoresis through 1% pulsed-field certified agarose (Bio-Rad, Richmond, CA, USA) by using a CHEF-Mapper (Bio-Rad).

    Techniques: Pulsed-Field Gel, Electrophoresis

    Dendrogram of 38 XbaI-digested S. dysenteriae isolates based on the cluster analysis of PFGE patterns. The dendrogram was constructed using the UPGMA clustering method. The PFGE pattern and background information for each strain are listed on the right side of the dendrogram

    Journal: BMC Microbiology

    Article Title: Epidemic and molecular characterization of fluoroquinolone-resistant Shigella dysenteriae 1 isolates from calves with diarrhea

    doi: 10.1186/s12866-020-02050-9

    Figure Lengend Snippet: Dendrogram of 38 XbaI-digested S. dysenteriae isolates based on the cluster analysis of PFGE patterns. The dendrogram was constructed using the UPGMA clustering method. The PFGE pattern and background information for each strain are listed on the right side of the dendrogram

    Article Snippet: Intact agarose-embedded chromosomal DNA of S. dysenteriae isolates was digested with restriction enzyme XbaI (TaKaRa, Japan), and restriction fragments were separated on the CHEF Mapper XA system (Bio-Rad) in 1% SeaKem Gold agarose gel (Lonza, USA) in 0.5x TBE buffer with a size range of 30–700 kb.

    Techniques: Construct

    Dendrograms generated by Bionumerics software showing the cluster analysis of XbaI-PFGE patterns of CTX-M (A)- and CMY-2 (B)-producing E. coli strains isolated from stray dogs. Similarity analysis was performed by using the Dice coefficient, and clustering

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Molecular Characterization of Extended-Spectrum-?-Lactamase-Producing and Plasmid-Mediated AmpC ?-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea

    doi: 10.1128/AAC.05598-11

    Figure Lengend Snippet: Dendrograms generated by Bionumerics software showing the cluster analysis of XbaI-PFGE patterns of CTX-M (A)- and CMY-2 (B)-producing E. coli strains isolated from stray dogs. Similarity analysis was performed by using the Dice coefficient, and clustering

    Article Snippet: Pulsed-field gel electrophoresis (PFGE) of XbaI (Takara Bio Inc., Shiga, Japan)-digested genomic DNA was carried out according to the CDC pulseNet standardized procedure , using a Chef Mapper apparatus (Bio-Rad Laboratories, Hercules, CA).

    Techniques: Generated, Software, Isolation

    Genetic relatedness of E. coli isolates with class 1 integrons indicated by XbaI -digested chromosomal DNA

    Journal: BMC Veterinary Research

    Article Title: Interrelationship between tetracycline resistance determinants, phylogenetic group affiliation and carriage of class 1 integrons in commensal Escherichia coli isolates from cattle farms

    doi: 10.1186/s12917-018-1661-3

    Figure Lengend Snippet: Genetic relatedness of E. coli isolates with class 1 integrons indicated by XbaI -digested chromosomal DNA

    Article Snippet: Briefly, following 18 to 20 h growth on TSA at 37 °C, genomic DNA was digested with 50 U XbaI (TaKaRa, Japan) for 2 h at 37 °C, then the DNA fragments were subsequently separated on a 1.0% SeaKem Gold agarose gel (Lonza, USA) in 0.5× Tris-borate-EDTA (TBE) buffer using a CHEFMapper gel apparatus (Bio-Rad Laboratories, California, USA).

    Techniques: