xbai  (Roche)


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    Structured Review

    Roche xbai
    Agarose gel of HindIII and <t>XbaI</t> -digested pcDNA3.1/ <t>Mtb32C/Mtb32N</t> . Lane 1: HindIII and Xba Idigested digested vector; the 1045 bp fragment consist of mtb32C and mtb32N ; lane 2: DNA size marker (1000 bp).
    Xbai, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai/product/Roche
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    xbai - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis"

    Article Title: Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis

    Journal: Reports of Biochemistry & Molecular Biology

    doi:

    Agarose gel of HindIII and XbaI -digested pcDNA3.1/ Mtb32C/Mtb32N . Lane 1: HindIII and Xba Idigested digested vector; the 1045 bp fragment consist of mtb32C and mtb32N ; lane 2: DNA size marker (1000 bp).
    Figure Legend Snippet: Agarose gel of HindIII and XbaI -digested pcDNA3.1/ Mtb32C/Mtb32N . Lane 1: HindIII and Xba Idigested digested vector; the 1045 bp fragment consist of mtb32C and mtb32N ; lane 2: DNA size marker (1000 bp).

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation, Marker

    2) Product Images from "Characterization of Multidrug-Resistant Typhoid Outbreaks in Kenya"

    Article Title: Characterization of Multidrug-Resistant Typhoid Outbreaks in Kenya

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.42.4.1477-1482.2004

    (Left panel) Gel picture showing two different digest patterns of MDR serovar Typhi from Kenya: PFGE pattern 1 (lanes 1 and 2) and PFGE pattern 2 (lanes 3 and 4) with SpeI restriction endonuclease. Lane M, 50-kb lambda molecular size ladder. (Right panel) Gel picture showing XbaI digest pattern I of MDR serovar Typhi from Kenya (lanes 1 to 3), a similar PFGE pattern of MDR serovar Typhi from South Africa (lane 4) but different from the digest patterns of MDR serovar Typhi from Hong Kong (lane 5), Pakistan (lane 6), and the fully sensitive serovar Typhi from 1988 to 1990 outbreaks in Kenya (lanes 7 to 9). Lane M, 50-kb lambda molecular size ladder.
    Figure Legend Snippet: (Left panel) Gel picture showing two different digest patterns of MDR serovar Typhi from Kenya: PFGE pattern 1 (lanes 1 and 2) and PFGE pattern 2 (lanes 3 and 4) with SpeI restriction endonuclease. Lane M, 50-kb lambda molecular size ladder. (Right panel) Gel picture showing XbaI digest pattern I of MDR serovar Typhi from Kenya (lanes 1 to 3), a similar PFGE pattern of MDR serovar Typhi from South Africa (lane 4) but different from the digest patterns of MDR serovar Typhi from Hong Kong (lane 5), Pakistan (lane 6), and the fully sensitive serovar Typhi from 1988 to 1990 outbreaks in Kenya (lanes 7 to 9). Lane M, 50-kb lambda molecular size ladder.

    Techniques Used:

    3) Product Images from "The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum"

    Article Title: The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0602590103

    Strategy for PfNT1 disruption by double crossover. ( A ) Gene disruption strategy. I, schematic representation of WT PfNT1 chromosomal locus; II, the plasmid vector pKEΔPfNT1 used for stable transformation of P. falciparum ; III, the proposed model of integration of the plasmid by double crossover into the PfNT1 chromosomal locus. ( B ) Southern blot analysis of XbaI (X) and XbaI plus HindIII (X+H) digested genomic DNA from WT and pfnt1 Δ parasites by using BSD-specific and PfNT1-specific probes (as indicated by the gray double-headed arrows in part III of A ).
    Figure Legend Snippet: Strategy for PfNT1 disruption by double crossover. ( A ) Gene disruption strategy. I, schematic representation of WT PfNT1 chromosomal locus; II, the plasmid vector pKEΔPfNT1 used for stable transformation of P. falciparum ; III, the proposed model of integration of the plasmid by double crossover into the PfNT1 chromosomal locus. ( B ) Southern blot analysis of XbaI (X) and XbaI plus HindIII (X+H) digested genomic DNA from WT and pfnt1 Δ parasites by using BSD-specific and PfNT1-specific probes (as indicated by the gray double-headed arrows in part III of A ).

    Techniques Used: Plasmid Preparation, Transformation Assay, Southern Blot

    4) Product Images from "Emergence of Salmonella enterica subsp. enterica serovar Chester in a rural area of Japan"

    Article Title: Emergence of Salmonella enterica subsp. enterica serovar Chester in a rural area of Japan

    Journal: The Journal of Veterinary Medical Science

    doi: 10.1292/jvms.20-0033

    Salmonella enterica subsp. enterica serovar Chester DNA fingerprints generated via pulsed-field gel electrophoresis (PFGE). (A) PFGE pattern digested with XbaI; (B) PFGE pattern digested with BlnI. Each fingerprint is shown as follows: S615, Case 1; S616, Case 2; CS16064, Case 3; CS16065, Case 4; CS16066, Case 5; CS16067, Case 6; S614, Case 7; CS16068, Case 8; CS16069, the dog owned by the family of case 5 and 6.
    Figure Legend Snippet: Salmonella enterica subsp. enterica serovar Chester DNA fingerprints generated via pulsed-field gel electrophoresis (PFGE). (A) PFGE pattern digested with XbaI; (B) PFGE pattern digested with BlnI. Each fingerprint is shown as follows: S615, Case 1; S616, Case 2; CS16064, Case 3; CS16065, Case 4; CS16066, Case 5; CS16067, Case 6; S614, Case 7; CS16068, Case 8; CS16069, the dog owned by the family of case 5 and 6.

    Techniques Used: Generated, Pulsed-Field Gel, Electrophoresis

    5) Product Images from "Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing"

    Article Title: Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv095

    J is inserted into some plasmids transfected in L. tarentolae . The 25.2S, 25.2L and 28.2 plasmids, shown in Figure 2 , were purified after amplification in L. tarentolae or E. coli . The DNA samples were digested with BamHI, EcoRI and XbaI, size-fractionated in a 0.7% agarose gel in 0.5×TBE, transferred to nitrocellulose and incubated with an anti-J antibody. ( A ) The plasmids 25.2L and 25.2S purified from L. tarentolae show specific bands recognized by the anti-J antibody. It is clear that J was not only formed in the inserted 2 kb 25.2L cSSR, but also in the adjacent 0.8 kb intergenic α-tubulin and the 1.7 kb α-tubulin-neo fragments. The small 25.2S insert fragment is not visible on the blot. No J-containing fragments are detected when the plasmids were isolated from E. coli or when the 28.2 plasmid was used. ( B ) J-containing DNA fragments are only detected after growth in the wild-type and not after growth in the JBP2-null L. tarentolae strain. This blot contains the 0.4 kb 25.2S insert band, albeit weak because these small fragments are lost during blotting. The 1.2 kb fragment in the 25.2S plasmid preparation is due to a modification of the BamHI site.
    Figure Legend Snippet: J is inserted into some plasmids transfected in L. tarentolae . The 25.2S, 25.2L and 28.2 plasmids, shown in Figure 2 , were purified after amplification in L. tarentolae or E. coli . The DNA samples were digested with BamHI, EcoRI and XbaI, size-fractionated in a 0.7% agarose gel in 0.5×TBE, transferred to nitrocellulose and incubated with an anti-J antibody. ( A ) The plasmids 25.2L and 25.2S purified from L. tarentolae show specific bands recognized by the anti-J antibody. It is clear that J was not only formed in the inserted 2 kb 25.2L cSSR, but also in the adjacent 0.8 kb intergenic α-tubulin and the 1.7 kb α-tubulin-neo fragments. The small 25.2S insert fragment is not visible on the blot. No J-containing fragments are detected when the plasmids were isolated from E. coli or when the 28.2 plasmid was used. ( B ) J-containing DNA fragments are only detected after growth in the wild-type and not after growth in the JBP2-null L. tarentolae strain. This blot contains the 0.4 kb 25.2S insert band, albeit weak because these small fragments are lost during blotting. The 1.2 kb fragment in the 25.2S plasmid preparation is due to a modification of the BamHI site.

    Techniques Used: Transfection, Purification, Amplification, Agarose Gel Electrophoresis, Incubation, Isolation, Plasmid Preparation, Modification

    Maps of the plasmids containing convergent strand switch regions and telomeric repeats. The cSSRs and telomeric repeats were cloned into the HindIII and XbaI restriction sites of the 5.5 kb pGEM 7Zf α-neo-α backbone before transfection into wild-type L. tarentolae . The gene coding for neomycin phosphotransferase (yellow), which confers resistance to paromomycin, served as a selection marker after transfection. The intergenic α-tubulin gene fragments flanking the neomycin marker are indicated in light blue. The 25.2S plasmid has a 381 bp insert corresponding to the cSSR with a J-peak in chromosome 25 of L. tarentolae (position 412 392–412 772). The 25.2L plasmid contains a 2.0 kb insert covering the 25.2S region (positions 410 965–412 966). The plasmid 28.2 contains a 2.4 kb fragment. This fragment corresponds to the cSSR in chromosome 28 of L. tarentolae which contains no J in the wild-type (position 580 566–582 966). The telomeric sequence plasmids contain 10 copies of the wild-type telomeric repeat (GGGTTA) or a mutant version (GGGTTT). Plasmids were linearized with ScaI for gel extraction and SMRT sequencing. Plasmids were digested with BamHI, EcoRI and XbaI for DNA immunoblots.
    Figure Legend Snippet: Maps of the plasmids containing convergent strand switch regions and telomeric repeats. The cSSRs and telomeric repeats were cloned into the HindIII and XbaI restriction sites of the 5.5 kb pGEM 7Zf α-neo-α backbone before transfection into wild-type L. tarentolae . The gene coding for neomycin phosphotransferase (yellow), which confers resistance to paromomycin, served as a selection marker after transfection. The intergenic α-tubulin gene fragments flanking the neomycin marker are indicated in light blue. The 25.2S plasmid has a 381 bp insert corresponding to the cSSR with a J-peak in chromosome 25 of L. tarentolae (position 412 392–412 772). The 25.2L plasmid contains a 2.0 kb insert covering the 25.2S region (positions 410 965–412 966). The plasmid 28.2 contains a 2.4 kb fragment. This fragment corresponds to the cSSR in chromosome 28 of L. tarentolae which contains no J in the wild-type (position 580 566–582 966). The telomeric sequence plasmids contain 10 copies of the wild-type telomeric repeat (GGGTTA) or a mutant version (GGGTTT). Plasmids were linearized with ScaI for gel extraction and SMRT sequencing. Plasmids were digested with BamHI, EcoRI and XbaI for DNA immunoblots.

    Techniques Used: Clone Assay, Transfection, Selection, Marker, Plasmid Preparation, Sequencing, Mutagenesis, Gel Extraction, Western Blot

    6) Product Images from "Molecular and Biochemical Characterization of the Natural Chromosome-Encoded Class A ?-Lactamase from Pseudomonas luteola ▿"

    Article Title: Molecular and Biochemical Characterization of the Natural Chromosome-Encoded Class A ?-Lactamase from Pseudomonas luteola ▿

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00427-09

    Pulsed-field gel electrophoresis of SpeI-digested genomic DNA from P. luteola . Lanes 1 and 8, XbaI-digested genomic DNA from Salmonella enterica serotype Braenderup H9812, used to provide molecular size markers (band sizes in kilobases); lane 2, clinical
    Figure Legend Snippet: Pulsed-field gel electrophoresis of SpeI-digested genomic DNA from P. luteola . Lanes 1 and 8, XbaI-digested genomic DNA from Salmonella enterica serotype Braenderup H9812, used to provide molecular size markers (band sizes in kilobases); lane 2, clinical

    Techniques Used: Pulsed-Field Gel, Electrophoresis

    Related Articles

    Clone Assay:

    Article Title: Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
    Article Snippet: .. Cloning of L. tarentolae DNA segments into plasmids and plasmid isolation The Leishmania expression vector pGEM 7Zf α-neo-α ( ) was digested with HindIII and XbaI, gel extracted, phenol purified, and dephosphorylated with calf intestinal alkaline phosphatase (1 U/μl, Roche). .. The fragments to be inserted were obtained by polymerase chain reaction (PCR) amplification using L. tarentolae genomic DNA as a template, gel extracted and digested with HindIII and XbaI.

    Agarose Gel Electrophoresis:

    Article Title: The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum
    Article Snippet: .. Ten micrograms of genomic DNA from WT and pfnt1 Δ parasites was digested with XbaI and HindIII, separated on a 0.8% agarose gel, and transferred to positively charged nylon membrane (Roche Molecular Biochemicals). ..

    Ligation:

    Article Title: Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome
    Article Snippet: .. PCR products were purified with a Qiagen PCR purification kit and digested with XbaI and PstI (Roche Applied Science) for mfsT and with SalI and XbaI for sdtR , followed by ligation using the FastLink DNA ligase kit (Epicentre Biotechnologies) to similarly digested plasmid pCI372. .. Electrocompetent E. coli MKH13 and E. coli EPI300 were transformed with the ligation mixture and plated on LB agar plates containing 20 μg/ml Cm for selection.

    Isolation:

    Article Title: Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
    Article Snippet: .. Cloning of L. tarentolae DNA segments into plasmids and plasmid isolation The Leishmania expression vector pGEM 7Zf α-neo-α ( ) was digested with HindIII and XbaI, gel extracted, phenol purified, and dephosphorylated with calf intestinal alkaline phosphatase (1 U/μl, Roche). .. The fragments to be inserted were obtained by polymerase chain reaction (PCR) amplification using L. tarentolae genomic DNA as a template, gel extracted and digested with HindIII and XbaI.

    Article Title: Emergence of Salmonella enterica subsp. enterica serovar Chester in a rural area of Japan
    Article Snippet: .. PFGE and cluster analysis were performed using XbaI and BlnI restriction enzymes (Roche Diagnostics, Rotkreuz, Switzerland) to determine whether isolated strains were derived from a same genetic clone ofS . ..

    Labeling:

    Article Title: Thin-foil magnetic force system for high-numerical-aperture microscopy
    Article Snippet: .. The DNA was then cut with XbaI and triple labeled with biotinylated dUTP (Roche Molecular Biochemicals) and biotinylated dATP and dCTP (Invitrogen Corp., Carlsbad, CA) using the Klenow reaction. .. Chromatin was formed from the DNA by incorporation of yeast nucleosomes using high-salt extraction of S. cerevisiae nuclear extracts, followed by a gradually decreased salt concentration to assemble nucleosomes onto the DNA.

    Purification:

    Article Title: Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
    Article Snippet: .. Cloning of L. tarentolae DNA segments into plasmids and plasmid isolation The Leishmania expression vector pGEM 7Zf α-neo-α ( ) was digested with HindIII and XbaI, gel extracted, phenol purified, and dephosphorylated with calf intestinal alkaline phosphatase (1 U/μl, Roche). .. The fragments to be inserted were obtained by polymerase chain reaction (PCR) amplification using L. tarentolae genomic DNA as a template, gel extracted and digested with HindIII and XbaI.

    Article Title: Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis
    Article Snippet: .. The purified mtb32N PCR product and pcDNA3.1/ Mtb32C were digested with XbaI and EcoRI (Roche Company). .. The ligation mixture contained 2 units of T4 DNA ligase, 2 µl of T4 DNA ligase buffer, 3 μl of mtb32N (11 ng/µl), 5 µl of pcDNA3.1/ Mtb32C (19 ng/µl), and 8 μl of H2 O.

    Article Title: Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome
    Article Snippet: .. PCR products were purified with a Qiagen PCR purification kit and digested with XbaI and PstI (Roche Applied Science) for mfsT and with SalI and XbaI for sdtR , followed by ligation using the FastLink DNA ligase kit (Epicentre Biotechnologies) to similarly digested plasmid pCI372. .. Electrocompetent E. coli MKH13 and E. coli EPI300 were transformed with the ligation mixture and plated on LB agar plates containing 20 μg/ml Cm for selection.

    Plasmid Preparation:

    Article Title: Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
    Article Snippet: .. Cloning of L. tarentolae DNA segments into plasmids and plasmid isolation The Leishmania expression vector pGEM 7Zf α-neo-α ( ) was digested with HindIII and XbaI, gel extracted, phenol purified, and dephosphorylated with calf intestinal alkaline phosphatase (1 U/μl, Roche). .. The fragments to be inserted were obtained by polymerase chain reaction (PCR) amplification using L. tarentolae genomic DNA as a template, gel extracted and digested with HindIII and XbaI.

    Article Title: Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome
    Article Snippet: .. PCR products were purified with a Qiagen PCR purification kit and digested with XbaI and PstI (Roche Applied Science) for mfsT and with SalI and XbaI for sdtR , followed by ligation using the FastLink DNA ligase kit (Epicentre Biotechnologies) to similarly digested plasmid pCI372. .. Electrocompetent E. coli MKH13 and E. coli EPI300 were transformed with the ligation mixture and plated on LB agar plates containing 20 μg/ml Cm for selection.

    Expressing:

    Article Title: Defining the sequence requirements for the positioning of base J in DNA using SMRT sequencing
    Article Snippet: .. Cloning of L. tarentolae DNA segments into plasmids and plasmid isolation The Leishmania expression vector pGEM 7Zf α-neo-α ( ) was digested with HindIII and XbaI, gel extracted, phenol purified, and dephosphorylated with calf intestinal alkaline phosphatase (1 U/μl, Roche). .. The fragments to be inserted were obtained by polymerase chain reaction (PCR) amplification using L. tarentolae genomic DNA as a template, gel extracted and digested with HindIII and XbaI.

    Polymerase Chain Reaction:

    Article Title: Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis
    Article Snippet: .. The purified mtb32N PCR product and pcDNA3.1/ Mtb32C were digested with XbaI and EcoRI (Roche Company). .. The ligation mixture contained 2 units of T4 DNA ligase, 2 µl of T4 DNA ligase buffer, 3 μl of mtb32N (11 ng/µl), 5 µl of pcDNA3.1/ Mtb32C (19 ng/µl), and 8 μl of H2 O.

    Article Title: Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome
    Article Snippet: .. PCR products were purified with a Qiagen PCR purification kit and digested with XbaI and PstI (Roche Applied Science) for mfsT and with SalI and XbaI for sdtR , followed by ligation using the FastLink DNA ligase kit (Epicentre Biotechnologies) to similarly digested plasmid pCI372. .. Electrocompetent E. coli MKH13 and E. coli EPI300 were transformed with the ligation mixture and plated on LB agar plates containing 20 μg/ml Cm for selection.

    Derivative Assay:

    Article Title: Emergence of Salmonella enterica subsp. enterica serovar Chester in a rural area of Japan
    Article Snippet: .. PFGE and cluster analysis were performed using XbaI and BlnI restriction enzymes (Roche Diagnostics, Rotkreuz, Switzerland) to determine whether isolated strains were derived from a same genetic clone ofS . ..

    Pulsed-Field Gel:

    Article Title: Molecular and Biochemical Characterization of the Natural Chromosome-Encoded Class A ?-Lactamase from Pseudomonas luteola ▿
    Article Snippet: .. The genetic diversity of P. luteola was assessed by pulsed-field gel electrophoresis (PFGE) of genomic DNA digested with XbaI or SpeI (Roche), as previously described ( ). .. The entire coding region of the bla LUT gene of all P. luteola strains was amplified by PCR, using the primers UpPlut (5′-ACCGTCTAGGCTGCTACTTCA-3′) and LoPlut (5′-CCGCTGCGCATGAGCGTA-3′), binding 200 bp upstream from the ATG initiation codon of LUT-1 and 10 bp downstream from the stop codon, respectively.

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  • xbai  (Roche)
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    Roche xbai
    Agarose gel of HindIII and <t>XbaI</t> -digested pcDNA3.1/ <t>Mtb32C/Mtb32N</t> . Lane 1: HindIII and Xba Idigested digested vector; the 1045 bp fragment consist of mtb32C and mtb32N ; lane 2: DNA size marker (1000 bp).
    Xbai, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai/product/Roche
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    xbai - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    88
    Roche restriction endonucleases xbai
    Dendrogram showing the genetic similarity between combined <t>XbaI-BlnI</t> profiles determined by using the unweighted-pair group method with arithmetic averages (UPGMA) and Jaccard's coefficient ( J ). The numbers in parentheses are the numbers of isolates when
    Restriction Endonucleases Xbai, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases xbai/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    91
    Roche xbai restriction enzyme
    a The differences in plasmid number from a selection of bacteria isolates. Several isolates contained up to eight plasmids b S1-PFGE and Southern blotting of plasmids carrying <t>NDM-1</t> from clinical isolates. Pulsed-field gel of S1-treated plasmid DNA of selected Enterobacteriaceae from clinical isolates stained with ethidium bromide. The molecular weight marker is S. braenderup H9812 cut with <t>XbaI</t> ( A ). Autodiagram of gel A showing plasmids carrying NDM-1 ( B )
    Xbai Restriction Enzyme, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai restriction enzyme/product/Roche
    Average 91 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    xbai restriction enzyme - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Agarose gel of HindIII and XbaI -digested pcDNA3.1/ Mtb32C/Mtb32N . Lane 1: HindIII and Xba Idigested digested vector; the 1045 bp fragment consist of mtb32C and mtb32N ; lane 2: DNA size marker (1000 bp).

    Journal: Reports of Biochemistry & Molecular Biology

    Article Title: Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis

    doi:

    Figure Lengend Snippet: Agarose gel of HindIII and XbaI -digested pcDNA3.1/ Mtb32C/Mtb32N . Lane 1: HindIII and Xba Idigested digested vector; the 1045 bp fragment consist of mtb32C and mtb32N ; lane 2: DNA size marker (1000 bp).

    Article Snippet: The purified mtb32N PCR product and pcDNA3.1/ Mtb32C were digested with XbaI and EcoRI (Roche Company).

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Marker

    Pulsed field gel electrophoresis of XbaI-digested DNA from the 59 ExPEC strains included in the study . Strain designation, phylogenetic group, ST assignation, clinical and geographical origin of isolation, PFGE cluster ( > 85% similarity), and PCR result for virulence genes that exhibited significant differences within the pathogenic groups are shown at right. This unweighted pair-group method with arithmetic mean dendrogram was generated in BioNumerics software (Applied Maths, St-Martens-Latem, Belgium) by using Dice coefficient with a 1.0% band position tolerance. The scale above the dendrogram indicates percent similarity. AS: abdominal sepsis; UTI: urinary tract infection; CI: cystitis; IS: intestinal sepsis; NBM: Newborn Meningitis; US: urosepsis; P?: posible pyelonephritis; C: colibacillosis; RS: respiratory sepsis; AP: acute pyelonephritis; AB: asymptomatic bacteriuria.

    Journal: BMC Microbiology

    Article Title: Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution

    doi: 10.1186/1471-2180-9-132

    Figure Lengend Snippet: Pulsed field gel electrophoresis of XbaI-digested DNA from the 59 ExPEC strains included in the study . Strain designation, phylogenetic group, ST assignation, clinical and geographical origin of isolation, PFGE cluster ( > 85% similarity), and PCR result for virulence genes that exhibited significant differences within the pathogenic groups are shown at right. This unweighted pair-group method with arithmetic mean dendrogram was generated in BioNumerics software (Applied Maths, St-Martens-Latem, Belgium) by using Dice coefficient with a 1.0% band position tolerance. The scale above the dendrogram indicates percent similarity. AS: abdominal sepsis; UTI: urinary tract infection; CI: cystitis; IS: intestinal sepsis; NBM: Newborn Meningitis; US: urosepsis; P?: posible pyelonephritis; C: colibacillosis; RS: respiratory sepsis; AP: acute pyelonephritis; AB: asymptomatic bacteriuria.

    Article Snippet: Pulse Field Gel Electrophoresis (PFGE) Cleavage of the agarose-embedded DNA was achieved with 0.2 U/μl XbaI (Roche) according to instructions of the manufacturer.

    Techniques: Pulsed-Field Gel, Electrophoresis, Isolation, Polymerase Chain Reaction, Generated, Software, Infection

    Dendrogram showing the genetic similarity between combined XbaI-BlnI profiles determined by using the unweighted-pair group method with arithmetic averages (UPGMA) and Jaccard's coefficient ( J ). The numbers in parentheses are the numbers of isolates when

    Journal: Applied and Environmental Microbiology

    Article Title: A Predominant Multidrug-Resistant Salmonella enterica Serovar Saintpaul Clonal Line in German Turkey and Related Food Products ▿

    doi: 10.1128/AEM.02744-09

    Figure Lengend Snippet: Dendrogram showing the genetic similarity between combined XbaI-BlnI profiles determined by using the unweighted-pair group method with arithmetic averages (UPGMA) and Jaccard's coefficient ( J ). The numbers in parentheses are the numbers of isolates when

    Article Snippet: Macrorestriction analysis of genomic DNA was carried out for all isolates using restriction endonucleases XbaI and BlnI (Roche Diagnostics, Mannheim, Germany).

    Techniques:

    a The differences in plasmid number from a selection of bacteria isolates. Several isolates contained up to eight plasmids b S1-PFGE and Southern blotting of plasmids carrying NDM-1 from clinical isolates. Pulsed-field gel of S1-treated plasmid DNA of selected Enterobacteriaceae from clinical isolates stained with ethidium bromide. The molecular weight marker is S. braenderup H9812 cut with XbaI ( A ). Autodiagram of gel A showing plasmids carrying NDM-1 ( B )

    Journal: European Journal of Clinical Microbiology & Infectious Diseases

    Article Title: Common isolation of New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae in a large surgical hospital in Vietnam

    doi: 10.1007/s10096-015-2345-6

    Figure Lengend Snippet: a The differences in plasmid number from a selection of bacteria isolates. Several isolates contained up to eight plasmids b S1-PFGE and Southern blotting of plasmids carrying NDM-1 from clinical isolates. Pulsed-field gel of S1-treated plasmid DNA of selected Enterobacteriaceae from clinical isolates stained with ethidium bromide. The molecular weight marker is S. braenderup H9812 cut with XbaI ( A ). Autodiagram of gel A showing plasmids carrying NDM-1 ( B )

    Article Snippet: NDM-1-positive bacterial isolates were genotyped by pulsed-field gel electrophoresis (PFGE), using XbaI restriction enzyme (Roche Diagnostic, Mannheim, Germany) to digest the bacterial genomes in agarose blocks.

    Techniques: Plasmid Preparation, Selection, Southern Blot, Pulsed-Field Gel, Staining, Molecular Weight, Marker