xbai tctaga  (New England Biolabs)


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    Structured Review

    New England Biolabs xbai tctaga
    Xbai Tctaga, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai tctaga/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai tctaga - by Bioz Stars, 2020-04
    87/100 stars

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    spectinomycin resistance cassette

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    Clone Assay:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: Sequences of the cloned products were verified by Sanger sequencing. .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Agarose Gel Electrophoresis:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: The ligation reaction was loaded onto a 1% agarose gel, and a 1-kb band was purified using the Qiaquick gel extraction kit (Qiagen) and cloned into pCR2.1 TOPO cloning vector resulting in pCR2.1up5′+3′dn. .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Ligation:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: The ligation reaction was loaded onto a 1% agarose gel, and a 1-kb band was purified using the Qiaquick gel extraction kit (Qiagen) and cloned into pCR2.1 TOPO cloning vector resulting in pCR2.1up5′+3′dn. .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Mutagenesis:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: Paragraph title: Deletion mutant construction and complementation. ... The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Ethanol Precipitation:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII. .. Plasmids bearing mutant alleles were linearized by digesting with either KpnI (GGTACC) (NEB) or XhoI (CTCGAG) (NEB) and concentrated by ethanol precipitation.

    Purification:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: The ligation reaction was loaded onto a 1% agarose gel, and a 1-kb band was purified using the Qiaquick gel extraction kit (Qiagen) and cloned into pCR2.1 TOPO cloning vector resulting in pCR2.1up5′+3′dn. .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Sequencing:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: Sequences of the cloned products were verified by Sanger sequencing. .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Polymerase Chain Reaction:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: PCR products were digested with the AvrII (CCTAGG) (NEB, Beverly, MA) restriction endonuclease, ethanol precipitated, and ligated with the T4 DNA ligase (NEB). .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Gel Extraction:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: The ligation reaction was loaded onto a 1% agarose gel, and a 1-kb band was purified using the Qiaquick gel extraction kit (Qiagen) and cloned into pCR2.1 TOPO cloning vector resulting in pCR2.1up5′+3′dn. .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII.

    Recombinant:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII. .. The spectinomycin resistance cassette was cloned into pCR2.1up5′-′+3′dn to complete the construction of the mutant alleles.

    Plasmid Preparation:

    Article Title: Outer Cell Surface Components Essential for Fe(III) Oxide Reduction by Geobacter metallireducens
    Article Snippet: .. The spectinomycin resistance cassette was digested with XbaI (TCTAGA) (NEB) from pUC19- Sp r loxP , and the recombinant plasmid pCR2.1up5′+3′dn was digested with AvrII. .. The spectinomycin resistance cassette was cloned into pCR2.1up5′-′+3′dn to complete the construction of the mutant alleles.

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    New England Biolabs endogenous xbai sites tctaga
    Endogenous Xbai Sites Tctaga, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous xbai sites tctaga/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    endogenous xbai sites tctaga - by Bioz Stars, 2020-04
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      Buy from Supplier

    87
    New England Biolabs xbai tctaga
    Xbai Tctaga, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai tctaga/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai tctaga - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

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