xbai spei  (New England Biolabs)


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    Structured Review

    New England Biolabs xbai spei
    Xbai Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai spei/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai spei - by Bioz Stars, 2020-04
    85/100 stars

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    Sequencing:

    Article Title: Mutations in the SAM Domain of the ETV6-NTRK3 Chimeric Tyrosine Kinase Block Polymerization and Transformation Activity
    Article Snippet: FKBP1E-NTRK3 and FKBP2E-NTRK3 fusion constructs were created by amplifying the NTRK3 region of ETV6-NTRK3 (with the primer set NTRK3-1601-XbaI [5′-GCTCTAGAGATGTGCAGCACATTAAGAGG-3′] and NTRK3-2490rev-SpeI [5′-GGACTAGTGCCAAGAATGTCCAGG-3′]), cutting the resultant product with XbaI/SpeI (NEB), and fusing it in frame with pC4 M-FV 1E or pC4 M-FV 2E (renamed FKBP1E and FKBP2E, respectively). .. The fusion constructs were verified by sequence analysis.

    Clone Assay:

    Article Title: Mutations in the SAM Domain of the ETV6-NTRK3 Chimeric Tyrosine Kinase Block Polymerization and Transformation Activity
    Article Snippet: FKBP1E-NTRK3 and FKBP2E-NTRK3 fusion constructs were created by amplifying the NTRK3 region of ETV6-NTRK3 (with the primer set NTRK3-1601-XbaI [5′-GCTCTAGAGATGTGCAGCACATTAAGAGG-3′] and NTRK3-2490rev-SpeI [5′-GGACTAGTGCCAAGAATGTCCAGG-3′]), cutting the resultant product with XbaI/SpeI (NEB), and fusing it in frame with pC4 M-FV 1E or pC4 M-FV 2E (renamed FKBP1E and FKBP2E, respectively). .. An EcoRI/ApoI or partial EcoRI/ApoI digest facilitated cloning of the fusion constructs into the MSCVpuro retroviral vector (Clontech).

    Construct:

    Article Title: Mutations in the SAM Domain of the ETV6-NTRK3 Chimeric Tyrosine Kinase Block Polymerization and Transformation Activity
    Article Snippet: .. FKBP1E-NTRK3 and FKBP2E-NTRK3 fusion constructs were created by amplifying the NTRK3 region of ETV6-NTRK3 (with the primer set NTRK3-1601-XbaI [5′-GCTCTAGAGATGTGCAGCACATTAAGAGG-3′] and NTRK3-2490rev-SpeI [5′-GGACTAGTGCCAAGAATGTCCAGG-3′]), cutting the resultant product with XbaI/SpeI (NEB), and fusing it in frame with pC4 M-FV 1E or pC4 M-FV 2E (renamed FKBP1E and FKBP2E, respectively). .. The fusion constructs were verified by sequence analysis.

    Plasmid Preparation:

    Article Title: Mutations in the SAM Domain of the ETV6-NTRK3 Chimeric Tyrosine Kinase Block Polymerization and Transformation Activity
    Article Snippet: FKBP1E-NTRK3 and FKBP2E-NTRK3 fusion constructs were created by amplifying the NTRK3 region of ETV6-NTRK3 (with the primer set NTRK3-1601-XbaI [5′-GCTCTAGAGATGTGCAGCACATTAAGAGG-3′] and NTRK3-2490rev-SpeI [5′-GGACTAGTGCCAAGAATGTCCAGG-3′]), cutting the resultant product with XbaI/SpeI (NEB), and fusing it in frame with pC4 M-FV 1E or pC4 M-FV 2E (renamed FKBP1E and FKBP2E, respectively). .. An EcoRI/ApoI or partial EcoRI/ApoI digest facilitated cloning of the fusion constructs into the MSCVpuro retroviral vector (Clontech).

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    New England Biolabs restriction enzymes xbai spei
    Restriction Enzymes Xbai Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs xbai spei
    Xbai Spei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai spei/product/New England Biolabs
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    New England Biolabs xbai spei restriction sites 3
    Xbai Spei Restriction Sites 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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