xbai noti  (New England Biolabs)


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    Structured Review

    New England Biolabs xbai noti
    Xbai Noti, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai noti/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai noti - by Bioz Stars, 2020-04
    94/100 stars

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    Electroporation:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis . ..

    Functional Assay:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: Since CEN is essential to direct precise DNA segregation, isolation of a functional CEN is the goal of our future work. .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis .

    Isolation:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: Since CEN is essential to direct precise DNA segregation, isolation of a functional CEN is the goal of our future work. .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis .

    Expressing:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis . ..

    Sequencing:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: It was also reported that CEN sequence could greatly improve plasmid stability in S. cerevisiae , and the plasmid with CEN showed > 80% GFP expression ( ). .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis .

    Transformation Assay:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis . ..

    Plasmid Preparation:

    Article Title: Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
    Article Snippet: .. 10.1128/mSphere.00345-19.2 FIG S1 DNA transformations and GFP expression. (A) I. orientalis transformation by heat shock with 500 ng of pIo-UG. (B) I. orientalis transformation by heat shock with 500 ng of pIo-control (without ScARS). (C) E. coli transformation by electroporation with plasmid DNA extracted from 24-h and 120-h I. orientalis cultures. (D) Digestion confirmation of plasmid extracted from S1C by XbaI+NotI (two bands, 8 kb and 1.7 kb; ladder, New England BioLabs [NEB] 1 kb Plus DNA ladder). (E) Profiles of GFP expression at 24 h by ScARS and ScARS/CEN plasmids in I. orientalis . ..

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  • 94
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