xbai nonsense linker  (New England Biolabs)


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    Structured Review

    New England Biolabs xbai nonsense linker
    Xbai Nonsense Linker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai nonsense linker/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai nonsense linker - by Bioz Stars, 2020-03
    79/100 stars

    Related Products / Commonly Used Together

    hincii
    hap4p

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    Related Articles

    Clone Assay:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391. .. The PCR products were digested with BamHI/NotI and cloned into pCITE2c, and the plasmids were designated pDM401, pDM403, and pDM386.

    Amplification:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391. .. The truncated alleles HAP4 (1 - 210) , HAP4 (1 - 180) , and HAP4 (1 - 165) were generated by PCR amplification with pSLF413 ( ) as the template and the primer pairs oDM0076/oDM0128, oDM0076/oDM0135, and oDM0076/oDM0115, which incorporated unique 5′ and 3′ BamHI and NotI restriction sites, respectively, as well as a termination codon after the indicated amino acid residue.

    In Vitro:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: To delimit the region of Hap4p required for interaction with Hap2p/Hap3p/Hap5p, truncated alleles of HAP4 were generated in the plasmid pCITE2a for in vitro transcription/translation. .. It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391.

    Ligation:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: .. It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391. .. The truncated alleles HAP4 (1 - 210) , HAP4 (1 - 180) , and HAP4 (1 - 165) were generated by PCR amplification with pSLF413 ( ) as the template and the primer pairs oDM0076/oDM0128, oDM0076/oDM0135, and oDM0076/oDM0115, which incorporated unique 5′ and 3′ BamHI and NotI restriction sites, respectively, as well as a termination codon after the indicated amino acid residue.

    Introduce:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: .. It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391. .. The truncated alleles HAP4 (1 - 210) , HAP4 (1 - 180) , and HAP4 (1 - 165) were generated by PCR amplification with pSLF413 ( ) as the template and the primer pairs oDM0076/oDM0128, oDM0076/oDM0135, and oDM0076/oDM0115, which incorporated unique 5′ and 3′ BamHI and NotI restriction sites, respectively, as well as a termination codon after the indicated amino acid residue.

    Generated:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: To delimit the region of Hap4p required for interaction with Hap2p/Hap3p/Hap5p, truncated alleles of HAP4 were generated in the plasmid pCITE2a for in vitro transcription/translation. .. It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391.

    Polymerase Chain Reaction:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: The PCR products and pGEX-2T were digested with BamHI/EcoRI and ligated. .. It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391.

    Plasmid Preparation:

    Article Title: Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA Complex in Saccharomyces cerevisiae
    Article Snippet: .. It was used to generate Hap4p (1-250) by digestion with HincII and ligation with an XbaI nonsense linker (New England Biolabs, Inc.) to introduce a stop codon after amino acid residue 250, and the plasmid was designated pDM391. .. The truncated alleles HAP4 (1 - 210) , HAP4 (1 - 180) , and HAP4 (1 - 165) were generated by PCR amplification with pSLF413 ( ) as the template and the primer pairs oDM0076/oDM0128, oDM0076/oDM0135, and oDM0076/oDM0115, which incorporated unique 5′ and 3′ BamHI and NotI restriction sites, respectively, as well as a termination codon after the indicated amino acid residue.

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  • 79
    New England Biolabs xbai nonsense linker
    Xbai Nonsense Linker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai nonsense linker/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai nonsense linker - by Bioz Stars, 2020-03
    79/100 stars
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