xbai ncoi  (New England Biolabs)


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  • 93

    Structured Review

    New England Biolabs xbai ncoi
    Xbai Ncoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai ncoi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai ncoi - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Resurrecting the bacterial tyrosyl-tRNA synthetase/tRNA pair for expanding the genetic code of both E. coli and eukaryotes
    Article Snippet: .. The full length PCR product containing the MjYRS/tRNA was cloned into pUltra Hit14 via XbaI/NcoI (NEB), producing pUltraBR MjY. .. To generate the positive selection plasmid containing an ampicillin reporter, pRep-ScW14 was digested with SpeI. ( ) The 5’ fragment of the ampicillin resistance cassette (amplified from a pAcBac vector with Amp_NheI-F and Amp-2.3.InsTAG-R) was overlap-extended with the 3’ fragment (amplified with Amp-2.3.InsTAG-F and pacbac1-Amp_SpeI-R), and amplified using terminal primers Amp_NheI-F and pacbac1-Amp_SpeI-R.

    Clone Assay:

    Article Title: Resurrecting the bacterial tyrosyl-tRNA synthetase/tRNA pair for expanding the genetic code of both E. coli and eukaryotes
    Article Snippet: .. The full length PCR product containing the MjYRS/tRNA was cloned into pUltra Hit14 via XbaI/NcoI (NEB), producing pUltraBR MjY. .. To generate the positive selection plasmid containing an ampicillin reporter, pRep-ScW14 was digested with SpeI. ( ) The 5’ fragment of the ampicillin resistance cassette (amplified from a pAcBac vector with Amp_NheI-F and Amp-2.3.InsTAG-R) was overlap-extended with the 3’ fragment (amplified with Amp-2.3.InsTAG-F and pacbac1-Amp_SpeI-R), and amplified using terminal primers Amp_NheI-F and pacbac1-Amp_SpeI-R.

    Selection:

    Article Title: Resurrecting the bacterial tyrosyl-tRNA synthetase/tRNA pair for expanding the genetic code of both E. coli and eukaryotes
    Article Snippet: A previously developed but selection incompatible complementation plasmid, pBR MjY,( ) was used to amplify the MjYRS and MjY-tRNA with primers pBR.MjY-XbaI-F and MjYtR_RS.SphI-R. .. The full length PCR product containing the MjYRS/tRNA was cloned into pUltra Hit14 via XbaI/NcoI (NEB), producing pUltraBR MjY.

    Plasmid Preparation:

    Article Title: Resurrecting the bacterial tyrosyl-tRNA synthetase/tRNA pair for expanding the genetic code of both E. coli and eukaryotes
    Article Snippet: Paragraph title: Complementation plasmid pUltraBR MjY: ... The full length PCR product containing the MjYRS/tRNA was cloned into pUltra Hit14 via XbaI/NcoI (NEB), producing pUltraBR MjY.

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  • 93
    New England Biolabs xbai ncoi
    Xbai Ncoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai ncoi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai ncoi - by Bioz Stars, 2020-04
    93/100 stars
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