xbai hindiii  (New England Biolabs)


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    Structured Review

    New England Biolabs xbai hindiii
    Xbai Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai hindiii/product/New England Biolabs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    xbai hindiii - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: .. The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs). .. The correct in-frame cloning of grlA , as well as the presence of each mutation, was verified by DNA sequencing.

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: .. After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions. .. The MBP protein was expressed from unmodified plasmid pMAL-c2, for use as a negative control.

    Centrifugation:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs). .. Cells were harvested by centrifugation at 10,000 × g at 4°C and then washed once with column buffer (20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1 mM EDTA, 10 mM 2-mercaptoethanol), resuspended in 10 ml of the same buffer, and broken by sonication.

    Amplification:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: For constructing MBP-GrlA recombinant proteins, WT and mutant grlA genes were amplified by PCR using as templates DNA of the pTEPGrlA1-derived plasmids (Table ) and primers MBPCrgrlAF and EPCiOrf11R. .. The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs).

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: .. After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions. .. The MBP protein was expressed from unmodified plasmid pMAL-c2, for use as a negative control.

    Mutagenesis:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: For constructing MBP-GrlA recombinant proteins, WT and mutant grlA genes were amplified by PCR using as templates DNA of the pTEPGrlA1-derived plasmids (Table ) and primers MBPCrgrlAF and EPCiOrf11R. .. The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs).

    Negative Control:

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions. .. The MBP protein was expressed from unmodified plasmid pMAL-c2, for use as a negative control.

    Purification:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: .. The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs). .. The correct in-frame cloning of grlA , as well as the presence of each mutation, was verified by DNA sequencing.

    Recombinant:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: For constructing MBP-GrlA recombinant proteins, WT and mutant grlA genes were amplified by PCR using as templates DNA of the pTEPGrlA1-derived plasmids (Table ) and primers MBPCrgrlAF and EPCiOrf11R. .. The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs).

    DNA Sequencing:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs). .. The correct in-frame cloning of grlA , as well as the presence of each mutation, was verified by DNA sequencing.

    Expressing:

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: .. After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions. .. The MBP protein was expressed from unmodified plasmid pMAL-c2, for use as a negative control.

    Polymerase Chain Reaction:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: .. The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs). .. The correct in-frame cloning of grlA , as well as the presence of each mutation, was verified by DNA sequencing.

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: Rv0496 (MTB-PPX1) and Rv1026 The rv0496 and rv1026 genes were PCR amplified from M. tuberculosis H37Rv genomic DNA using the Rv0496for2 and Rv0496rev2, and Rv1026for and Rv1026rev primer pairs, respectively, with the use of LongAmp Taq DNA polymerase from New England Biolabs (NEB). .. After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions.

    Sonication:

    Article Title: Molecular Characterization of GrlA, a Specific Positive Regulator of ler Expression in Enteropathogenic Escherichia coli ▿
    Article Snippet: The PCR products were purified from agarose gels, digested with XbaI-HindIII, and cloned in frame at the 3′ end of the malE gene in pMAL-c2x (New England Biolabs). .. Cells were harvested by centrifugation at 10,000 × g at 4°C and then washed once with column buffer (20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1 mM EDTA, 10 mM 2-mercaptoethanol), resuspended in 10 ml of the same buffer, and broken by sonication.

    Binding Assay:

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: .. After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions. .. The MBP protein was expressed from unmodified plasmid pMAL-c2, for use as a negative control.

    Plasmid Preparation:

    Article Title: The Two PPX-GppA Homologues from Mycobacterium tuberculosis Have Distinct Biochemical Activities
    Article Snippet: After TOPO cloning (pCR2.1 TOPO-TA cloning kit from Invitrogen, Life Technologies), amplified genes were subcloned (XbaI/HindIII for Rv0496 , BamHI/HindIII for Rv1026 ), into similarly digested pMAL-c2 expression vectors (NEB), to encode N-terminal maltose binding protein (MBP; MalE) fusions. .. The MBP protein was expressed from unmodified plasmid pMAL-c2, for use as a negative control.

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  • 99
    New England Biolabs xbai hindiii restriction enzymes
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