xbai digested pmal c2 vector  (New England Biolabs)


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    New England Biolabs xbai digested pmal c2 vector
    Xbai Digested Pmal C2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai digested pmal c2 vector/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai digested pmal c2 vector - by Bioz Stars, 2020-03
    79/100 stars

    Related Products / Commonly Used Together

    xmni blunt end
    pcr amplicons

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    Related Articles

    Clone Assay:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: .. PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ). .. Escherichia coli JM109 cells were used for amplifying and screening the recombinant plasmids, and protease-deficient BL21 E. coli cells were used for protein expression.

    Amplification:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: For bacterial fusion protein expression, the capsid protein genes of individual SV strains were amplified from cDNA clones with strain-specific primers containing restriction enzyme sites for in-frame cloning. .. PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ).

    Purification:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: .. PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ). .. Escherichia coli JM109 cells were used for amplifying and screening the recombinant plasmids, and protease-deficient BL21 E. coli cells were used for protein expression.

    Expressing:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: Paragraph title: Bacterial fusion protein expression and purification. ... PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ).

    Polymerase Chain Reaction:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: .. PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ). .. Escherichia coli JM109 cells were used for amplifying and screening the recombinant plasmids, and protease-deficient BL21 E. coli cells were used for protein expression.

    Recombinant:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ). .. Escherichia coli JM109 cells were used for amplifying and screening the recombinant plasmids, and protease-deficient BL21 E. coli cells were used for protein expression.

    Binding Assay:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ). .. Maltose binding protein (MBP)-capsid fusion protein clones were grown and induced under the same conditions, except rich medium (10 g/liter tryptone, 5 g/liter yeast extract, 5 g/liter NaCl, 2 g/liter glucose, 100 μg/ml ampicillin) was used.

    Plasmid Preparation:

    Article Title: Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children
    Article Snippet: .. PCR amplicons were gel purified, digested with the appropriate restriction enzyme(s), and in frame cloned into XmnI (blunt end)- and XbaI-digested pMAL-c2 vector (New England BioLabs, Beverly, MA) or BamHI- and NotI-digested (in the case of Hou7-1181, EcoRI and NotI) pGEX-4T-1 vector (Amersham, Piscataway, NJ). .. Escherichia coli JM109 cells were used for amplifying and screening the recombinant plasmids, and protease-deficient BL21 E. coli cells were used for protein expression.

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  • 79
    New England Biolabs xbai digested pmal c2 vector
    Xbai Digested Pmal C2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai digested pmal c2 vector/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai digested pmal c2 vector - by Bioz Stars, 2020-03
    79/100 stars
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