wt terminal labeling kit  (Thermo Fisher)


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    Name:
    HT WT Terminal Labeling and Controls Kit
    Description:
    The HT WT Terminal Labeling and Controls Kit is used with the WT Expression Kit for High Throughput Robotics sold separately Both products can be combined with the HT WT Expression Assay Consumables for Biomek FXp Target Prep Express sold seaprately for customers who use the Beckman Coulter Biomek FXp Target Prep Express Instrument The HT WT Terminal Labeling and Controls Kit contains the GeneChip HT WT Terminal Labeling Kit GeneChip Hybridization Control Kit and GeneChipPoly A Control Kit Key Features • Low RNA input requirements as little as 100 ng total RNA for a single round of amplification • No separate rRNA depletion step • Magnetic bead purification for enhanced recovery and ease of use • StreamLined workflow for simplified protocol and reduced hands on time • Unbiased coverage of the transcriptome The WT Expression Kit for High Throughput Robotics includes • All reagents needed to generate sense cDNA ready for fragmentation and labeling • Magnetic beads for enhanced purification efficiency The HT WT Terminal Labeling and Controls Kit includes • WT Fragmentation and Labeling Kit required for target produced by the WT Expression Kit for High Throughput Robotics • Poly A controls are added to input RNA to measure efficiency of target amplification by the WT Expression Kit for High Throughput Robotics • Hybridization controls that monitor the hybridization process for troubleshooting purposes Related LinksHT WT Expression Assay Consumables for Biomek FXp Target Prep ExpressHT WT Terminal Labeling and Controls Kit
    Catalog Number:
    901647
    Price:
    None
    Applications:
    DNA & RNA Microarray Analysis|Microarray Analysis
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher wt terminal labeling kit
    The HT WT Terminal Labeling and Controls Kit is used with the WT Expression Kit for High Throughput Robotics sold separately Both products can be combined with the HT WT Expression Assay Consumables for Biomek FXp Target Prep Express sold seaprately for customers who use the Beckman Coulter Biomek FXp Target Prep Express Instrument The HT WT Terminal Labeling and Controls Kit contains the GeneChip HT WT Terminal Labeling Kit GeneChip Hybridization Control Kit and GeneChipPoly A Control Kit Key Features • Low RNA input requirements as little as 100 ng total RNA for a single round of amplification • No separate rRNA depletion step • Magnetic bead purification for enhanced recovery and ease of use • StreamLined workflow for simplified protocol and reduced hands on time • Unbiased coverage of the transcriptome The WT Expression Kit for High Throughput Robotics includes • All reagents needed to generate sense cDNA ready for fragmentation and labeling • Magnetic beads for enhanced purification efficiency The HT WT Terminal Labeling and Controls Kit includes • WT Fragmentation and Labeling Kit required for target produced by the WT Expression Kit for High Throughput Robotics • Poly A controls are added to input RNA to measure efficiency of target amplification by the WT Expression Kit for High Throughput Robotics • Hybridization controls that monitor the hybridization process for troubleshooting purposes Related LinksHT WT Expression Assay Consumables for Biomek FXp Target Prep ExpressHT WT Terminal Labeling and Controls Kit
    https://www.bioz.com/result/wt terminal labeling kit/product/Thermo Fisher
    Average 99 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    wt terminal labeling kit - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Glucosamine Downregulates the IL-1β-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms
    Article Snippet: .. Single-stranded cDNA was generated from the in vitro transcribed cRNA using an Ambion® WT Expression kit (Life Technologies, Carlsbad, CA, USA), and then fragmented and biotinylated with a WT Terminal Labeling Kit (Affymetrix Inc., Santa Clara, CA, USA), according to the manufacturer’s instruction. .. The labeled cDNA was hybridized with a Human Gene 1.0 ST Array, which contained probe sets of 28,869 annotated genes (Affimetrix® Inc., Santa Clara, CA, USA).

    Isolation:

    Article Title: Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits CD8+ T cell memory formation
    Article Snippet: .. Microarray analysis Total RNA was isolated from 106 Id3 −/− or Id3 +/+ and 3 × 105 Id3Thy-1.1 or Thy-1.1 pmel-1 CD8+ T cells sorted 5 days after infection using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Mouse Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix). .. Microarrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).

    Infection:

    Article Title: Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits CD8+ T cell memory formation
    Article Snippet: .. Microarray analysis Total RNA was isolated from 106 Id3 −/− or Id3 +/+ and 3 × 105 Id3Thy-1.1 or Thy-1.1 pmel-1 CD8+ T cells sorted 5 days after infection using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Mouse Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix). .. Microarrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).

    Microarray:

    Article Title: Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits CD8+ T cell memory formation
    Article Snippet: .. Microarray analysis Total RNA was isolated from 106 Id3 −/− or Id3 +/+ and 3 × 105 Id3Thy-1.1 or Thy-1.1 pmel-1 CD8+ T cells sorted 5 days after infection using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Mouse Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix). .. Microarrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).

    Generated:

    Article Title: Altered physiological functions and ion currents in atrial fibroblasts from patients with chronic atrial fibrillation. Altered physiological functions and ion currents in atrial fibroblasts from patients with chronic atrial fibrillation
    Article Snippet: .. Subsequently, 5.5 μ g of this generated single‐strand DNA was fragmented with a combination of uracil DNA glucosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE1) and then labeled using the WT terminal labeling kit (Affymetrix Inc., Santa Clara, CA, USA). .. The biotinylated DNA was added to a hybridization cocktail for hybridization to the Human Gene 1.0ST Array, also containing 50 pM control oligonucleotide B2, eukaryotic hybridization controls (bioB, bioC, bioD, 1.5, 5, 25 pM, respectively), according to the manufacturer's protocol (Hybridization Wash and Stain Kit, Affymetrix).

    Labeling:

    Article Title: RAF1-MEK1-ERK/AKT axis may confer NSCLC cell lines resistance to erlotinib
    Article Snippet: .. 5.5 μg 2nd-cycle cDNA was fragmented and the single-stranded cDNA was labeled with GeneChip2 WT Terminal Labeling Kit and Controls Kit (Affymetrix, PN 702880). .. About 700 ng fragmented and labeled single-stranded cDNA were hybridized to an Affymetrix GeneChip Human Gene 1.0 ST array, which was washed and stained with GeneChip2 Hybridization, Wash and Stain kit (Affymetrix).

    Article Title: Programmed death-1 receptor signaling downregulates asparaginyl endopeptidase to maintain Foxp3 stability in induced Regulatory T cells
    Article Snippet: .. Sense strand cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit. .. Four replicates of each group were hybridized to Affymetrix mouse Gene ST 2.0 GeneChip in Affymetrix hybridization oven at 45° C, 60RPM for 16 hrs.

    Article Title: Altered physiological functions and ion currents in atrial fibroblasts from patients with chronic atrial fibrillation. Altered physiological functions and ion currents in atrial fibroblasts from patients with chronic atrial fibrillation
    Article Snippet: .. Subsequently, 5.5 μ g of this generated single‐strand DNA was fragmented with a combination of uracil DNA glucosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE1) and then labeled using the WT terminal labeling kit (Affymetrix Inc., Santa Clara, CA, USA). .. The biotinylated DNA was added to a hybridization cocktail for hybridization to the Human Gene 1.0ST Array, also containing 50 pM control oligonucleotide B2, eukaryotic hybridization controls (bioB, bioC, bioD, 1.5, 5, 25 pM, respectively), according to the manufacturer's protocol (Hybridization Wash and Stain Kit, Affymetrix).

    Article Title: Synthetic cytokine receptors transmit biological signals using artificial ligands
    Article Snippet: .. For this, total RNA (150 ng) was processed using the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Waltham, MA, USA) and hybridized on Affymetrix Mouse Gene ST 1.0 arrays containing about 28,000 probe sets. ..

    Article Title: Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits CD8+ T cell memory formation
    Article Snippet: .. Microarray analysis Total RNA was isolated from 106 Id3 −/− or Id3 +/+ and 3 × 105 Id3Thy-1.1 or Thy-1.1 pmel-1 CD8+ T cells sorted 5 days after infection using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Mouse Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix). .. Microarrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).

    Expressing:

    Article Title: Glucosamine Downregulates the IL-1β-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms
    Article Snippet: .. Single-stranded cDNA was generated from the in vitro transcribed cRNA using an Ambion® WT Expression kit (Life Technologies, Carlsbad, CA, USA), and then fragmented and biotinylated with a WT Terminal Labeling Kit (Affymetrix Inc., Santa Clara, CA, USA), according to the manufacturer’s instruction. .. The labeled cDNA was hybridized with a Human Gene 1.0 ST Array, which contained probe sets of 28,869 annotated genes (Affimetrix® Inc., Santa Clara, CA, USA).

    Article Title: Synthetic cytokine receptors transmit biological signals using artificial ligands
    Article Snippet: .. For this, total RNA (150 ng) was processed using the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Waltham, MA, USA) and hybridized on Affymetrix Mouse Gene ST 1.0 arrays containing about 28,000 probe sets. ..

    Article Title: Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits CD8+ T cell memory formation
    Article Snippet: .. Microarray analysis Total RNA was isolated from 106 Id3 −/− or Id3 +/+ and 3 × 105 Id3Thy-1.1 or Thy-1.1 pmel-1 CD8+ T cells sorted 5 days after infection using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Mouse Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix). .. Microarrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).

    Article Title: Inactivation of SREBP-1a Phosphorylation Prevents Fatty Liver Disease in Mice: Identification of Related Signaling Pathways by Gene Expression Profiles in Liver and Proteomes of Peroxisomes
    Article Snippet: .. Genome wide expression analyses (n = 8 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labelling Kit (Affymetrix, Freiburg, Germany). .. All protocol steps were monitored using a RNA 6000 nano kit (Agilent, Taufkirchen, Germany).

    Genome Wide:

    Article Title: Inactivation of SREBP-1a Phosphorylation Prevents Fatty Liver Disease in Mice: Identification of Related Signaling Pathways by Gene Expression Profiles in Liver and Proteomes of Peroxisomes
    Article Snippet: .. Genome wide expression analyses (n = 8 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labelling Kit (Affymetrix, Freiburg, Germany). .. All protocol steps were monitored using a RNA 6000 nano kit (Agilent, Taufkirchen, Germany).

    High Throughput Screening Assay:

    Article Title: GSK-3 modulates SHH-driven proliferation in postnatal cerebellar neurogenesis and medulloblastoma
    Article Snippet: .. Samples were prepared using the Ambion WT Expression Kit for High-Throughput Robotics (4440537) and the Affymetrix HT WT Terminal Labeling and Controls Kit (901647). .. We quantified transcripts using the Affymetrix Mouse Gene 2.1 ST 24-Arrays (902140, Affymetrix) and scanned with the Affymetrix GeneChip Scanner 3000 7G Plus.

    Staining:

    Article Title: Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits CD8+ T cell memory formation
    Article Snippet: .. Microarray analysis Total RNA was isolated from 106 Id3 −/− or Id3 +/+ and 3 × 105 Id3Thy-1.1 or Thy-1.1 pmel-1 CD8+ T cells sorted 5 days after infection using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Mouse Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix). .. Microarrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).

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  • News
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  • 99
    Thermo Fisher wt terminal labeling kit
    Wt Terminal Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt terminal labeling kit/product/Thermo Fisher
    Average 99 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    wt terminal labeling kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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