wt expression kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    WT Expression Kit
    Description:
    The WT Expression Kit is an RNA amplification kit that is designed to generate amplified sense strand cDNA ready for fragmentation and labeling using the Affymetrix GeneChip WT Terminal Labeling Kit Features of the WT Expression Kit • Optimized for use with Affymetrix GeneChip Human Mouse and Rat 1 0 ST Arrays• Allows RNA samples as small as 50 ng to be analyzed on Affymetrix GeneChip Arrays• Supports a streamlined workflow that does not require a separate rRNA depletion step• Is ideal for high sensitivity expression profilingEliminates separate rRNA depletion step preserves transcriptome coverageThe first generation Affymetrix amplification method requires depletion of ribosomal RNA rRNA from RNA samples for optimal exon level analysis Conversely the Ambion WT Expression Kit uses a novel reverse transcription RT priming method that eliminates the need for a separate rRNA depletion step The kit includes RT primers designed using a proprietary oligonucleotide matching algorithm that eliminates primer sequences with homology to known ribosomal RNAs The result is complete and unbiased coverage of the transcriptome with rRNA amplification levels significantly lower than those of other methods Consistent results from less input RNAWith the Ambion WT Expression Kit samples as small as 50 ng of total RNA can be analyzed on Affymetrix GeneChip Human Mouse and Rat Exon and Gene 1 0 ST Arrays Previous methods required 1 µg of total RNA often difficult or impossible to obtain from limited sources such as stem cells or small tissue samples The modest requirement for input RNA permits the analysis of rare samples and provides for more efficient and cost effective experimentation for most sample types To demonstrate the performance of the WT Expression Kit RNA from three sample types HeLa cells and Microarray Quality Control MAQC A and B samples was prepared in triplicate using either the Affymetrix GeneChip WT cDNA Synthesis and Amplification Kit or the WT Expression Kit and analyzed on Human Exon 1 0 ST Arrays Total RNA 1 µg prepared by the Affymetrix protocol underwent an rRNA depletion step while just 50 ng total RNA 20 fold less was prepared for microarray analysis using the WT Expression Kit Both sets of samples were handled according to the manufacturers recommendations Direct correlation of log2 ratios for MAQC samples A and B was high r 0 94 at both the exon and transcript levels Correlation of log2 ratios with gene expression data obtained using TaqMan Gene Expression assays for real time PCR was also high for both kits see Product Bulletin for data Identify more differential expression with less RNAIn addition to its lower input RNA requirement the WT Expression Kit provides a significant increase in sensitivity A greater number of probe sets detected above background was obtained at the exon level with the WT Expression Kit as a result of an increased signal to noise ratio A comparison of differential expression analysis results from the two methods indicated that while differentially expressed genes and exons of MAQC A and MAQC B samples were similar between the two kits 78 and 89 respectively significantly more differential expression was observed on arrays hybridized with samples prepared using the WT Expression Kit 499 more genes and 6 850 more exons were found to be differentially expressed in samples prepared using the Ambion kit compared to samples prepared with the Affymetrix kit Note For manual use only Not for robotics
    Catalog Number:
    4411973
    Price:
    None
    Applications:
    RNAi, Epigenetics & Non-Coding RNA Research|miRNA & Non-Coding RNA Analysis
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher wt expression kit
    The WT Expression Kit is an RNA amplification kit that is designed to generate amplified sense strand cDNA ready for fragmentation and labeling using the Affymetrix GeneChip WT Terminal Labeling Kit Features of the WT Expression Kit • Optimized for use with Affymetrix GeneChip Human Mouse and Rat 1 0 ST Arrays• Allows RNA samples as small as 50 ng to be analyzed on Affymetrix GeneChip Arrays• Supports a streamlined workflow that does not require a separate rRNA depletion step• Is ideal for high sensitivity expression profilingEliminates separate rRNA depletion step preserves transcriptome coverageThe first generation Affymetrix amplification method requires depletion of ribosomal RNA rRNA from RNA samples for optimal exon level analysis Conversely the Ambion WT Expression Kit uses a novel reverse transcription RT priming method that eliminates the need for a separate rRNA depletion step The kit includes RT primers designed using a proprietary oligonucleotide matching algorithm that eliminates primer sequences with homology to known ribosomal RNAs The result is complete and unbiased coverage of the transcriptome with rRNA amplification levels significantly lower than those of other methods Consistent results from less input RNAWith the Ambion WT Expression Kit samples as small as 50 ng of total RNA can be analyzed on Affymetrix GeneChip Human Mouse and Rat Exon and Gene 1 0 ST Arrays Previous methods required 1 µg of total RNA often difficult or impossible to obtain from limited sources such as stem cells or small tissue samples The modest requirement for input RNA permits the analysis of rare samples and provides for more efficient and cost effective experimentation for most sample types To demonstrate the performance of the WT Expression Kit RNA from three sample types HeLa cells and Microarray Quality Control MAQC A and B samples was prepared in triplicate using either the Affymetrix GeneChip WT cDNA Synthesis and Amplification Kit or the WT Expression Kit and analyzed on Human Exon 1 0 ST Arrays Total RNA 1 µg prepared by the Affymetrix protocol underwent an rRNA depletion step while just 50 ng total RNA 20 fold less was prepared for microarray analysis using the WT Expression Kit Both sets of samples were handled according to the manufacturers recommendations Direct correlation of log2 ratios for MAQC samples A and B was high r 0 94 at both the exon and transcript levels Correlation of log2 ratios with gene expression data obtained using TaqMan Gene Expression assays for real time PCR was also high for both kits see Product Bulletin for data Identify more differential expression with less RNAIn addition to its lower input RNA requirement the WT Expression Kit provides a significant increase in sensitivity A greater number of probe sets detected above background was obtained at the exon level with the WT Expression Kit as a result of an increased signal to noise ratio A comparison of differential expression analysis results from the two methods indicated that while differentially expressed genes and exons of MAQC A and MAQC B samples were similar between the two kits 78 and 89 respectively significantly more differential expression was observed on arrays hybridized with samples prepared using the WT Expression Kit 499 more genes and 6 850 more exons were found to be differentially expressed in samples prepared using the Ambion kit compared to samples prepared with the Affymetrix kit Note For manual use only Not for robotics
    https://www.bioz.com/result/wt expression kit/product/Thermo Fisher
    Average 99 stars, based on 1017 article reviews
    Price from $9.99 to $1999.99
    wt expression kit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Interleukin-11 is a Marker for Both Cancer- and Inflammation-Associated Fibroblasts that Contribute to Colorectal Cancer Progression
    Article Snippet: .. Purified RNAs were subjected to a LiCl purification to remove residual DSS. cDNA was synthesized with the Ambion WT Expression Kit (Affymetrix) and labeled with GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer’s protocol. .. Affymetrix GeneChip Hybridization wash stain kit was used to hybridize samples to GeneChip Mouse Gene ST 1.0 ST Array (Affymetrix).

    Isolation:

    Article Title: Spaceflight Activates Autophagy Programs and the Proteasome in Mouse Liver
    Article Snippet: .. Briefly, RNA was isolated using an RNeasy kit (Qiagen, Germantown, MD, USA) and the Ambion WT expression kit (Thermo Fisher Scientific, Waltham, MA, USA) was employed to prepare mRNA for whole transcriptome microarray analysis using an Affymetrix GeneChip 1.0 ST array (Thermo Fisher Scientific). .. Arrays were scanned using a GeneChip Scanner 3000 7G (Thermo Fisher Scientific) and Command Console Software v. 3.2.3 (Thermo Fisher Scientific) to produce.

    Labeling:

    Article Title: Inflammasome Activation by Cystine Crystals: Implications for the Pathogenesis of Cystinosis
    Article Snippet: .. The Ambion WT Expression Kit was used to generate double-stranded biotinylated cDNA, and the Affymetrix HT WT Terminal Labeling Kit was used to prepare the cDNA for hybridization to Affymetrix GeneChip Mouse Gene 1.1 ST arrays (Affymetrix, Santa Clara, CA). .. The double-stranded biotinylated cDNA from each tissue for each mouse was run on individual Affymetrix GeneChip.

    Article Title: Interleukin-11 is a Marker for Both Cancer- and Inflammation-Associated Fibroblasts that Contribute to Colorectal Cancer Progression
    Article Snippet: .. Purified RNAs were subjected to a LiCl purification to remove residual DSS. cDNA was synthesized with the Ambion WT Expression Kit (Affymetrix) and labeled with GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer’s protocol. .. Affymetrix GeneChip Hybridization wash stain kit was used to hybridize samples to GeneChip Mouse Gene ST 1.0 ST Array (Affymetrix).

    Article Title: Exercise training improves liver steatosis in mice
    Article Snippet: .. Purified RNA (100 ng) was labeled with the Ambion WT expression kit (Invitrogen) and hybridized to an Affymetrix Mouse Gene 1.1 ST array plate (Affymetrix, Santa Clara, CA). .. Hybridization, washing, and scanning were carried out on an Affymetrix GeneTitan platform according to the instruction by the manufacturer.

    Purification:

    Article Title: Interleukin-11 is a Marker for Both Cancer- and Inflammation-Associated Fibroblasts that Contribute to Colorectal Cancer Progression
    Article Snippet: .. Purified RNAs were subjected to a LiCl purification to remove residual DSS. cDNA was synthesized with the Ambion WT Expression Kit (Affymetrix) and labeled with GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer’s protocol. .. Affymetrix GeneChip Hybridization wash stain kit was used to hybridize samples to GeneChip Mouse Gene ST 1.0 ST Array (Affymetrix).

    Article Title: Exercise training improves liver steatosis in mice
    Article Snippet: .. Purified RNA (100 ng) was labeled with the Ambion WT expression kit (Invitrogen) and hybridized to an Affymetrix Mouse Gene 1.1 ST array plate (Affymetrix, Santa Clara, CA). .. Hybridization, washing, and scanning were carried out on an Affymetrix GeneTitan platform according to the instruction by the manufacturer.

    Microarray:

    Article Title: Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity
    Article Snippet: .. For microarray whole transcriptome analysis, 100 ng RNA was required for reverse transcription, cDNA preparation using the Ambion WT Expression kit, which is a standard protocol in Genechip hybridization facilities. ..

    Article Title: Spaceflight Activates Autophagy Programs and the Proteasome in Mouse Liver
    Article Snippet: .. Briefly, RNA was isolated using an RNeasy kit (Qiagen, Germantown, MD, USA) and the Ambion WT expression kit (Thermo Fisher Scientific, Waltham, MA, USA) was employed to prepare mRNA for whole transcriptome microarray analysis using an Affymetrix GeneChip 1.0 ST array (Thermo Fisher Scientific). .. Arrays were scanned using a GeneChip Scanner 3000 7G (Thermo Fisher Scientific) and Command Console Software v. 3.2.3 (Thermo Fisher Scientific) to produce.

    Expressing:

    Article Title: Inflammasome Activation by Cystine Crystals: Implications for the Pathogenesis of Cystinosis
    Article Snippet: .. The Ambion WT Expression Kit was used to generate double-stranded biotinylated cDNA, and the Affymetrix HT WT Terminal Labeling Kit was used to prepare the cDNA for hybridization to Affymetrix GeneChip Mouse Gene 1.1 ST arrays (Affymetrix, Santa Clara, CA). .. The double-stranded biotinylated cDNA from each tissue for each mouse was run on individual Affymetrix GeneChip.

    Article Title: Interleukin-11 is a Marker for Both Cancer- and Inflammation-Associated Fibroblasts that Contribute to Colorectal Cancer Progression
    Article Snippet: .. Purified RNAs were subjected to a LiCl purification to remove residual DSS. cDNA was synthesized with the Ambion WT Expression Kit (Affymetrix) and labeled with GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer’s protocol. .. Affymetrix GeneChip Hybridization wash stain kit was used to hybridize samples to GeneChip Mouse Gene ST 1.0 ST Array (Affymetrix).

    Article Title: Exercise training improves liver steatosis in mice
    Article Snippet: .. Purified RNA (100 ng) was labeled with the Ambion WT expression kit (Invitrogen) and hybridized to an Affymetrix Mouse Gene 1.1 ST array plate (Affymetrix, Santa Clara, CA). .. Hybridization, washing, and scanning were carried out on an Affymetrix GeneTitan platform according to the instruction by the manufacturer.

    Article Title: Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes
    Article Snippet: .. Using the Ambion WT Expression Kit, per sample, an amount of 50 ng of total RNA spiked with bacterial poly-A RNA positive controls (Affymetrix) was converted to double-stranded cDNA in a reverse transcription reaction. .. Real-time quantitative polymerase chain reactions (RT-qPCRs) were performed with 10 ng cDNA in SYBR Green I mix and run on a ViiA 7 Real-Time PCR System (Applied Biosystems).

    Article Title: Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity
    Article Snippet: .. For microarray whole transcriptome analysis, 100 ng RNA was required for reverse transcription, cDNA preparation using the Ambion WT Expression kit, which is a standard protocol in Genechip hybridization facilities. ..

    Article Title: Spaceflight Activates Autophagy Programs and the Proteasome in Mouse Liver
    Article Snippet: .. Briefly, RNA was isolated using an RNeasy kit (Qiagen, Germantown, MD, USA) and the Ambion WT expression kit (Thermo Fisher Scientific, Waltham, MA, USA) was employed to prepare mRNA for whole transcriptome microarray analysis using an Affymetrix GeneChip 1.0 ST array (Thermo Fisher Scientific). .. Arrays were scanned using a GeneChip Scanner 3000 7G (Thermo Fisher Scientific) and Command Console Software v. 3.2.3 (Thermo Fisher Scientific) to produce.

    Article Title: AKT/ERK activation is associated with gastric cancer cell resistance to paclitaxel
    Article Snippet: .. The above steps were performed with the Ambion WT Expression Kit. .. Second-cycle cDNA (5.5 μg) was fragmented, and the single-stranded cDNA was labeled with the GeneChip2 WT Terminal Labeling Kit and Controls Kit (Affymetrix, PN 702880).

    Article Title: Gene Expression Profiling To Predict Viridans Group Streptococcal and Invasive Fungal Infection in Pediatric Acute Myeloid Leukemia: A Brief Report from the Children’s Oncology Group
    Article Snippet: .. All protocols were conducted as described in the Ambion® WT Expression Kit for Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays manual. .. Baseline characteristics of case and control groups were compared by Kruskal Wallis equality of proportions rank test for continuous variables and Fisher’s exact test for categorical variables, using STATA statistical software v. 12.0 (College Station, TX).

    Hybridization:

    Article Title: Inflammasome Activation by Cystine Crystals: Implications for the Pathogenesis of Cystinosis
    Article Snippet: .. The Ambion WT Expression Kit was used to generate double-stranded biotinylated cDNA, and the Affymetrix HT WT Terminal Labeling Kit was used to prepare the cDNA for hybridization to Affymetrix GeneChip Mouse Gene 1.1 ST arrays (Affymetrix, Santa Clara, CA). .. The double-stranded biotinylated cDNA from each tissue for each mouse was run on individual Affymetrix GeneChip.

    Article Title: Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity
    Article Snippet: .. For microarray whole transcriptome analysis, 100 ng RNA was required for reverse transcription, cDNA preparation using the Ambion WT Expression kit, which is a standard protocol in Genechip hybridization facilities. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher wt expression kit
    Wt Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1064 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt expression kit/product/Thermo Fisher
    Average 99 stars, based on 1064 article reviews
    Price from $9.99 to $1999.99
    wt expression kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results