wt expression kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher wt expression kit
    Wt Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt expression kit/product/Thermo Fisher
    Average 99 stars, based on 777 article reviews
    Price from $9.99 to $1999.99
    wt expression kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Hybridization:

    Article Title: The effects of n-6 polyunsaturated fatty acid deprivation on the inflammatory gene response to lipopolysaccharide in the mouse hippocampus
    Article Snippet: As previously described [ ], extracted RNA was reverse transcribed with a WT Expression Kit (ThermoFisher), then single-stranded complementary DNA was fragmented and labeled according to the Affymetrix WT fragmentation and labelling protocol. cDNA was hybridized to the arrays with an Affymetrix Mouse Gene 2.0 ST GeneChip (ThermoFisher) for 18 h at 45 °C at 60 RPM to process it for analysis. .. Hybridization controls were similar across all arrays, indicating successful hybridization.

    Article Title: Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma
    Article Snippet: A total of 250 ng of total RNA was amplified into cRNA and made into cDNA using the Ambion WT Expression Kit. .. The labeled fragmented cDNA was added into the hybridization cocktail that was prepared according to the protocol included in the Affymetrix GeneTitan Hybridization Wash and Stain kit.

    Article Title: Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer
    Article Snippet: Briefly 200 ng of RNA amplified sense‐strand cDNA was generated using the Ambion® WT Expression Kit (Life Technologies, Carlsbad, CA, USA). .. This was then fragmented and labelled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix) before hybridization onto the arrays and subsequent scanning on the GeneTitan system.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA). .. The arrays were placed in the hybridization oven for 17 h at 45 °C, and then washed and stained in the GeneChip Fluidics Station 450 (Affymetrix).

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit. .. Then the labeled cRNAs were hybridized, washed and stained in GeneChip® Hybridization, Wash, and Stain Kit.

    Article Title: Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life
    Article Snippet: 100 ng of total RNA from each sample was amplified with the Ambion WT Expression Kit (Life Technologies, Carlsbad, CA, USA) and hybridized to the GeneChip Mouse Gene 2.0 ST array (Affymetrix, Santa Clara, CA, USA), following manufacturer’s standard protocols. .. Hybridized arrays were washed and stained using the GeneChip Hybridization Wash and Stain Kit with a Fluidics Station 450 (standard protocols, using the AGCC Fluidics Control) and scanned with a GeneChip Scanner 3000 7G (standard settings, AGCC Scan Control, Affymetrix).

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: .. Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix). .. Processed RNA was hybridized to the Mouse Gene 1.0 ST Array (Affymetrix).

    RNA Extraction:

    Article Title: Fatty Liver Due to Increased de novo Lipogenesis: Alterations in the Hepatic Peroxisomal Proteome
    Article Snippet: Gene Expression Analyses RNA extraction (Qiagen, Hilden, Germany) of snap frozen liver biopsies was performed as described ( ). .. Genome wide expression analyses (n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labelling Kit (Thermo Fisher Scientific, Darmstadt, Germany).

    Article Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations
    Article Snippet: Paragraph title: RNA extraction and microarray analysis ... Genome-wide expression analyses ( n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Darmstadt, Germany).

    Amplification:

    Article Title: Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma
    Article Snippet: .. A total of 250 ng of total RNA was amplified into cRNA and made into cDNA using the Ambion WT Expression Kit. .. A total of 5.5 µg of the cDNA was then fragmented using the Affymetrix GeneChip WT Terminal Labeling kit and confirmed by running on the Agilent Bioanalyzer using the RNA 6000 kit.

    Article Title: Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer
    Article Snippet: .. Briefly 200 ng of RNA amplified sense‐strand cDNA was generated using the Ambion® WT Expression Kit (Life Technologies, Carlsbad, CA, USA). .. This was then fragmented and labelled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix) before hybridization onto the arrays and subsequent scanning on the GeneTitan system.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: .. First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA). .. In a second step, cDNA fragmentation and labeling were carried out using the Affymetrix GeneChip WT Terminal Labeling Kit.

    Article Title: Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life
    Article Snippet: .. 100 ng of total RNA from each sample was amplified with the Ambion WT Expression Kit (Life Technologies, Carlsbad, CA, USA) and hybridized to the GeneChip Mouse Gene 2.0 ST array (Affymetrix, Santa Clara, CA, USA), following manufacturer’s standard protocols. .. Hybridized arrays were washed and stained using the GeneChip Hybridization Wash and Stain Kit with a Fluidics Station 450 (standard protocols, using the AGCC Fluidics Control) and scanned with a GeneChip Scanner 3000 7G (standard settings, AGCC Scan Control, Affymetrix).

    Stable Transfection:

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: HepG2 stable cell lines of shSND1-#1 and pLKO-Vector were treated with 6 μg/mL 5-Fu. .. Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.).

    Synthesized:

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: .. Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit. .. Then the labeled cRNAs were hybridized, washed and stained in GeneChip® Hybridization, Wash, and Stain Kit.

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: .. Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.). .. Affymetrix Human Transcriptome Array 2.0 was then utilized to hybridize the fragmented cRNA product.

    Isolation:

    Article Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations
    Article Snippet: For microarray analysis of the pancreatic islets, the quality of the isolated RNA was tested using an RNA 6000 nano kit (Agilent Technologies, Taufkirchen, Germany). .. Genome-wide expression analyses ( n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Darmstadt, Germany).

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: LncRNA microarray analysis Total RNAs were isolated with Trizol from CD44+CD24- and non-CD44+CD24- cells derived from human breast cancer cell line MCF-7; and isolated from 3 paired poorly differentiated breast cancer tissues (tumor grade III) and adjacent normal tissues. .. Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit.

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: .. Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix). .. Processed RNA was hybridized to the Mouse Gene 1.0 ST Array (Affymetrix).

    Next-Generation Sequencing:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Genome-Wide Microarray Analysis of mRNAs Microarrays experiments were performed for a previous study [ ] on LCLs from a sample of 10 patients also included in the NGS study. .. First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA).

    Labeling:

    Article Title: The effects of n-6 polyunsaturated fatty acid deprivation on the inflammatory gene response to lipopolysaccharide in the mouse hippocampus
    Article Snippet: .. As previously described [ ], extracted RNA was reverse transcribed with a WT Expression Kit (ThermoFisher), then single-stranded complementary DNA was fragmented and labeled according to the Affymetrix WT fragmentation and labelling protocol. cDNA was hybridized to the arrays with an Affymetrix Mouse Gene 2.0 ST GeneChip (ThermoFisher) for 18 h at 45 °C at 60 RPM to process it for analysis. .. Hybridization controls were similar across all arrays, indicating successful hybridization.

    Article Title: Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma
    Article Snippet: A total of 250 ng of total RNA was amplified into cRNA and made into cDNA using the Ambion WT Expression Kit. .. A total of 5.5 µg of the cDNA was then fragmented using the Affymetrix GeneChip WT Terminal Labeling kit and confirmed by running on the Agilent Bioanalyzer using the RNA 6000 kit.

    Article Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations
    Article Snippet: .. Genome-wide expression analyses ( n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. All protocol steps were monitored using an RNA 6000 nano kit (Agilent).

    Article Title: Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer
    Article Snippet: Briefly 200 ng of RNA amplified sense‐strand cDNA was generated using the Ambion® WT Expression Kit (Life Technologies, Carlsbad, CA, USA). .. This was then fragmented and labelled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix) before hybridization onto the arrays and subsequent scanning on the GeneTitan system.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA). .. In a second step, cDNA fragmentation and labeling were carried out using the Affymetrix GeneChip WT Terminal Labeling Kit.

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: .. Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit. .. Then the labeled cRNAs were hybridized, washed and stained in GeneChip® Hybridization, Wash, and Stain Kit.

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: .. Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.). .. Affymetrix Human Transcriptome Array 2.0 was then utilized to hybridize the fragmented cRNA product.

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: .. Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix). .. Processed RNA was hybridized to the Mouse Gene 1.0 ST Array (Affymetrix).

    Purification:

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: Then, 48 h after treatment, we extracted and purified total RNA using TRIzol reagent (Life Technologies) and RNeasy mini kit (Qiagen). .. Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.).

    Microarray:

    Article Title: The effects of n-6 polyunsaturated fatty acid deprivation on the inflammatory gene response to lipopolysaccharide in the mouse hippocampus
    Article Snippet: Paragraph title: Microarray analysis ... As previously described [ ], extracted RNA was reverse transcribed with a WT Expression Kit (ThermoFisher), then single-stranded complementary DNA was fragmented and labeled according to the Affymetrix WT fragmentation and labelling protocol. cDNA was hybridized to the arrays with an Affymetrix Mouse Gene 2.0 ST GeneChip (ThermoFisher) for 18 h at 45 °C at 60 RPM to process it for analysis.

    Article Title: Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma
    Article Snippet: Paragraph title: Microarray analyses ... A total of 250 ng of total RNA was amplified into cRNA and made into cDNA using the Ambion WT Expression Kit.

    Article Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations
    Article Snippet: Paragraph title: RNA extraction and microarray analysis ... Genome-wide expression analyses ( n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Darmstadt, Germany).

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Paragraph title: 4.4. Genome-Wide Microarray Analysis of mRNAs ... First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: Paragraph title: LncRNA microarray analysis ... Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit.

    Article Title: Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life
    Article Snippet: Paragraph title: 2.10. Microarray analysis ... 100 ng of total RNA from each sample was amplified with the Ambion WT Expression Kit (Life Technologies, Carlsbad, CA, USA) and hybridized to the GeneChip Mouse Gene 2.0 ST array (Affymetrix, Santa Clara, CA, USA), following manufacturer’s standard protocols.

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: Paragraph title: RNA microarray detection ... Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.).

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: Paragraph title: Microarray and qRT-PCR ... Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix).

    Generated:

    Article Title: Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer
    Article Snippet: .. Briefly 200 ng of RNA amplified sense‐strand cDNA was generated using the Ambion® WT Expression Kit (Life Technologies, Carlsbad, CA, USA). .. This was then fragmented and labelled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix) before hybridization onto the arrays and subsequent scanning on the GeneTitan system.

    Spectrophotometry:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: The quality of RNA was considered to be adequate when the A260/A280 ratio assessed using a NanoDrop ND-1000 spectrophotometer was in the range of 1.8–2.0, and the RNA integrity number assessed using the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) was in the range of 7–10. .. First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA).

    Expressing:

    Article Title: The effects of n-6 polyunsaturated fatty acid deprivation on the inflammatory gene response to lipopolysaccharide in the mouse hippocampus
    Article Snippet: .. As previously described [ ], extracted RNA was reverse transcribed with a WT Expression Kit (ThermoFisher), then single-stranded complementary DNA was fragmented and labeled according to the Affymetrix WT fragmentation and labelling protocol. cDNA was hybridized to the arrays with an Affymetrix Mouse Gene 2.0 ST GeneChip (ThermoFisher) for 18 h at 45 °C at 60 RPM to process it for analysis. .. Hybridization controls were similar across all arrays, indicating successful hybridization.

    Article Title: Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma
    Article Snippet: .. A total of 250 ng of total RNA was amplified into cRNA and made into cDNA using the Ambion WT Expression Kit. .. A total of 5.5 µg of the cDNA was then fragmented using the Affymetrix GeneChip WT Terminal Labeling kit and confirmed by running on the Agilent Bioanalyzer using the RNA 6000 kit.

    Article Title: Fatty Liver Due to Increased de novo Lipogenesis: Alterations in the Hepatic Peroxisomal Proteome
    Article Snippet: .. Genome wide expression analyses (n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labelling Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. All protocol steps were monitored using an RNA 6000 nano kit (Agilent, Taufkirchen, Germany).

    Article Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations
    Article Snippet: .. Genome-wide expression analyses ( n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. All protocol steps were monitored using an RNA 6000 nano kit (Agilent).

    Article Title: Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer
    Article Snippet: .. Briefly 200 ng of RNA amplified sense‐strand cDNA was generated using the Ambion® WT Expression Kit (Life Technologies, Carlsbad, CA, USA). .. This was then fragmented and labelled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix) before hybridization onto the arrays and subsequent scanning on the GeneTitan system.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: .. First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA). .. In a second step, cDNA fragmentation and labeling were carried out using the Affymetrix GeneChip WT Terminal Labeling Kit.

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: .. Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit. .. Then the labeled cRNAs were hybridized, washed and stained in GeneChip® Hybridization, Wash, and Stain Kit.

    Article Title: Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life
    Article Snippet: .. 100 ng of total RNA from each sample was amplified with the Ambion WT Expression Kit (Life Technologies, Carlsbad, CA, USA) and hybridized to the GeneChip Mouse Gene 2.0 ST array (Affymetrix, Santa Clara, CA, USA), following manufacturer’s standard protocols. .. Hybridized arrays were washed and stained using the GeneChip Hybridization Wash and Stain Kit with a Fluidics Station 450 (standard protocols, using the AGCC Fluidics Control) and scanned with a GeneChip Scanner 3000 7G (standard settings, AGCC Scan Control, Affymetrix).

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: .. Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.). .. Affymetrix Human Transcriptome Array 2.0 was then utilized to hybridize the fragmented cRNA product.

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: .. Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix). .. Processed RNA was hybridized to the Mouse Gene 1.0 ST Array (Affymetrix).

    Genome Wide:

    Article Title: Fatty Liver Due to Increased de novo Lipogenesis: Alterations in the Hepatic Peroxisomal Proteome
    Article Snippet: .. Genome wide expression analyses (n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labelling Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. All protocol steps were monitored using an RNA 6000 nano kit (Agilent, Taufkirchen, Germany).

    Article Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations
    Article Snippet: .. Genome-wide expression analyses ( n = 5 per genotype) were performed with 150 ng RNA according to the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Thermo Fisher Scientific, Darmstadt, Germany). .. All protocol steps were monitored using an RNA 6000 nano kit (Agilent).

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Paragraph title: 4.4. Genome-Wide Microarray Analysis of mRNAs ... First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA).

    FACS:

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: .. Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix). .. Processed RNA was hybridized to the Mouse Gene 1.0 ST Array (Affymetrix).

    Quantitative RT-PCR:

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: Paragraph title: Microarray and qRT-PCR ... Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix).

    Software:

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit. .. At last, Robust Multichip Analysis (RMA) normalization for gene level analyses was completed by Expression Console (version 1.3.1, Affymetrix) software.

    Article Title: Lhx2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth
    Article Snippet: Total RNA from either epidermal keratinocytes of five control or five ΔN embryos at E14.5, or of FACS-purified EGFP-positive (placode) or EGFP-negative (IFE) epidermal keratinocytes from κ-EGFP embryos at E14.5 (see above) was isolated using the Absolutely RNA Microprep Kit (Agilent Technologies) and then processed with the WT Expression Kit (Ambion) and the WT Terminal Labeling and Hybridization Kit (Affymetrix). .. Five biological replicates for each sample were analyzed statistically with the multi-factor ANOVA test using Partek Genomic Suites software (Partek).

    Derivative Assay:

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: LncRNA microarray analysis Total RNAs were isolated with Trizol from CD44+CD24- and non-CD44+CD24- cells derived from human breast cancer cell line MCF-7; and isolated from 3 paired poorly differentiated breast cancer tissues (tumor grade III) and adjacent normal tissues. .. Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit.

    Staining:

    Article Title: Differential gene expression identifies KRT7 and MUC1 as potential metastasis-specific targets in sarcoma
    Article Snippet: A total of 250 ng of total RNA was amplified into cRNA and made into cDNA using the Ambion WT Expression Kit. .. The labeled fragmented cDNA was added into the hybridization cocktail that was prepared according to the protocol included in the Affymetrix GeneTitan Hybridization Wash and Stain kit.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: First, 100 ng of total RNA were amplified and used to generate a sense-strand complementary DNA (cDNA) with incorporated 2’-Deoxyuridine 5’-Triphosphate (dUTP), using the Ambion WT Expression Kit (Applied Biosystems, Foster City, CA, USA). .. The arrays were placed in the hybridization oven for 17 h at 45 °C, and then washed and stained in the GeneChip Fluidics Station 450 (Affymetrix).

    Article Title: LncCCAT1 Promotes Breast Cancer Stem Cell Function through Activating WNT/β-catenin Signaling
    Article Snippet: Briefly, total RNAs were transcribed to double strand cDNA by using The Ambion® WT Expression Kit, then synthesized into cRNA and labeled with Cyanine-3-CTP by using WT Terminal Labeling and Controls Kit. .. Then the labeled cRNAs were hybridized, washed and stained in GeneChip® Hybridization, Wash, and Stain Kit.

    Article Title: Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life
    Article Snippet: 100 ng of total RNA from each sample was amplified with the Ambion WT Expression Kit (Life Technologies, Carlsbad, CA, USA) and hybridized to the GeneChip Mouse Gene 2.0 ST array (Affymetrix, Santa Clara, CA, USA), following manufacturer’s standard protocols. .. Hybridized arrays were washed and stained using the GeneChip Hybridization Wash and Stain Kit with a Fluidics Station 450 (standard protocols, using the AGCC Fluidics Control) and scanned with a GeneChip Scanner 3000 7G (standard settings, AGCC Scan Control, Affymetrix).

    Article Title: SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma
    Article Snippet: Next, we synthesized the cDNA using an Ambion WT Expression Kit (Affymetrix), and obtained the cRNA by using a GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc.). .. After washing and staining in the GeneChip Fluidics Station 450, we used the Affymetrix® GeneChip Command Console (AGCC) and robust multichip analysis (RMA) algorithms to scan the arrays and analyze the data, under the Affymetrix default settings and normalization procedures (global scaling).

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  • 90
    Thermo Fisher wt expression kit
    Wt Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 777 article reviews
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    wt expression kit - by Bioz Stars, 2020-01
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