Structured Review

Promega wizardtm pcr clean up kit
Wizardtm Pcr Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizardtm pcr clean up kit/product/Promega
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
wizardtm pcr clean up kit - by Bioz Stars, 2020-04
92/100 stars

Related Products / Commonly Used Together

proteinase k
protein/dna complexes
reverse crosslinking
nacl

Images

Related Articles

SYBR Green Assay:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA). .. The H3K27me3 binding region in the promoter of ZEB1 or NEUROD1 was further detected using SYBR Green quantitative PCR method by the primer sets described by Chaffer CL et al. [ ].

Purification:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: .. Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA). .. The H3K27me3 binding region in the promoter of ZEB1 or NEUROD1 was further detected using SYBR Green quantitative PCR method by the primer sets described by Chaffer CL et al. [ ].

Real-time Polymerase Chain Reaction:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA). .. The H3K27me3 binding region in the promoter of ZEB1 or NEUROD1 was further detected using SYBR Green quantitative PCR method by the primer sets described by Chaffer CL et al. [ ].

Incubation:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: After the pre-clear step, 1 g of anti-H3K27me3 antibody or normal rabbit IgG was added to the cell lysate, and the mixture was incubated at 4°C overnight. .. Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA).

Polymerase Chain Reaction:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: .. Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA). .. The H3K27me3 binding region in the promoter of ZEB1 or NEUROD1 was further detected using SYBR Green quantitative PCR method by the primer sets described by Chaffer CL et al. [ ].

Lysis:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: Chromatin immunoprecipitation Cells were harvested by trypsin-EDTA and fixed with 1% formaldehyde at room temperature for 10 min. After quenching the formaldehyde with 125 mM glycine, the cells were lysed with mammalian cell lysis buffer (Pierce, Thermo Fisher Scientific, Inc.) and treated with micrococcal nuclease (Fermentas, Thermo Fisher Scientific, Inc.) at 37°C for 20 min. .. Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA).

Binding Assay:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA). .. The H3K27me3 binding region in the promoter of ZEB1 or NEUROD1 was further detected using SYBR Green quantitative PCR method by the primer sets described by Chaffer CL et al. [ ].

Chromatin Immunoprecipitation:

Article Title: Resveratrol suppresses myofibroblast activity of human buccal mucosal fibroblasts through the epigenetic inhibition of ZEB1 expression
Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Reverse crosslinking of the protein/DNA complexes was performed by treating with 200 mM NaCl at 65°C for at least 5 hours, and the proteins were digested with proteinase K. The DNA was further purified using a WizardTM PCR Clean-Up kit (Promega, Madison, WI, USA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Promega wizardtm pcr clean up kit
    Wizardtm Pcr Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizardtm pcr clean up kit/product/Promega
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizardtm pcr clean up kit - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results