wizard plus sv minipreps dna purification system  (Promega)

 
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    Structured Review

    Promega wizard plus sv minipreps dna purification system
    Wizard Plus Sv Minipreps Dna Purification System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard plus sv minipreps dna purification system/product/Promega
    Average 99 stars, based on 245 article reviews
    Price from $9.99 to $1999.99
    wizard plus sv minipreps dna purification system - by Bioz Stars, 2019-10
    99/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: Paragraph title: CpG methylation analysis by bisulfite sequencing ... Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: Paragraph title: CpG methylation analysis by bisulfite sequencing ... Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Clone Assay:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Paragraph title: Cloning and generation of transgenic mice ... Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
    Article Snippet: Guide RNAs used to target 5′ ends of individual enhancers were cloned into Bbs I-digested pSpCas9(BB)-2A-GFP vectors, while sgRNAs targeting 3′ ends of each enhancer were cloned into Bbs I-digested pSpCas9(BB)-2A-Puro vectors. .. Colonies were picked and cultured, and plasmids extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The PCR products obtained were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c). .. The promoter sequence details of immune checkpoints and PD-L1 genes are shown in Additional file : Figure S2 A-F.

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR products were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c). .. The promoter regions amplified for CpG methylation profile in this study were as previously described [ ].

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: Purified products were cloned using the pGEM®-T Vector System (Promega, Madison, WI, USA) and transformed into bacteria, following the manufacturer's instructions. .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach
    Article Snippet: Paragraph title: 2.3. Cloning of the predicted genes ... The recombinant plasmids were extracted from the E. coli culture using a Wizard plus SV Minipreps DNA Purification System (Promega, Fitchburg, WI, USA).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction. .. For proof-reading PCR, Phusion™ High-Fidelity DNA Polymerase (Finnzymes, Espoo, Finland) was used with 5x Phusion HF Buffer supplied.

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: For each plasmid, 377 bp segment of rpoB gene containing the RRDR region was synthetically generated and then cloned into a 2507 bp pEX-K vector (Eurofins MWG Operon, Germany). .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: Paragraph title: Isolation and cloning of the FIP-Lrh cDNA ... White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Centrifugation:

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions.

    Amplification:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The Actb-TG construct was generated via PCR using primers designed such that they amplified the Actg1-TG plasmid with ACTB-specific overhangs. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: To make recombinant FLAG-PrxII protein, pENTR/D-TOPO-PRDX2 was N-terminally FLAG-tagged (pENTR/D-TOPO-N-FLAG-PRDX2) via PCR using primers that amplified the entire plasmid with FLAG sequence overhangs. .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The bisulfite-treated DNA was then subjected to PCR for the amplification of the promoter regions of PD-1, CTLA-4, TIM-3, and LAG-3 using Hot Start TaKaRa Taq DNA Polymerase (TaKaRa Bio, Shiga, Japan). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR was then performed on the bisulfite-treated DNA for amplification of the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 using hot start TaKaRa Taq DNA polymerase (TaKaRa Bio, Shiga, Japan). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: The IGHV regions were amplified using the following primers, VH primer (forward) 5′-SARGTBMAGCTGSAGSAGTCWGG-3′; CHμ primer (reverse) 5′-CAGATCTCTGTTTTTGCCTCGTA-3′; CHγ primer (reverse) 5′-ATGCAAGGCTTACACCACAATCC-3′; and CHα primer (reverse) 5′-TAATAGGAGGAGGAGGAGTAGGAC-3′ (Life Technologies). .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The Actb-TG construct was generated via PCR using primers designed such that they amplified the Actg1-TG plasmid with ACTB-specific overhangs. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: To make recombinant FLAG-PrxII protein, pENTR/D-TOPO-PRDX2 was N-terminally FLAG-tagged (pENTR/D-TOPO-N-FLAG-PRDX2) via PCR using primers that amplified the entire plasmid with FLAG sequence overhangs. .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach
    Article Snippet: Predicted gene sequences were amplified from the metagenomic DNA using KOD-FX DNA polymerase (Toyobo, Osaka, Japan) with PCR primers shown in . .. The recombinant plasmids were extracted from the E. coli culture using a Wizard plus SV Minipreps DNA Purification System (Promega, Fitchburg, WI, USA).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: Paragraph title: DNA isolation, manipulation and amplification ... The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: Specific PCR primers of forward FIP-f (5′-ATGCGATACACCCTGCTGACCG-3′; Tm = 61.4 °C) and reverse FIP-r (5′-CTAGTTCCACTGAGCG ACCTTGAAC-3′; Tm = 61.2 °C) were designed based on the putative coding genomic sequence of FIP-Lrh (Yap et al. ) and used for amplification of the FIP-Lrh cDNA in a total 25 μL reaction volume containing FIP-f and FIP-r (10 pmol each), 2 μL cDNA template, 1 U of Taq DNA polymerase, 0.2 mM dNTPs and 2.5 μL of 10x Taq buffer. .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Plasmid pBCVP2G5-luc-NSN, pBTdel279, pCDNA3-Fas carrying the Fas gene and shuttle plasmid pDC316 were transfected into Escherichia coli JM109 competent cells (Promega Corporation, Madison, WI, USA) using Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h, according to the manufacturer's protocol. .. Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation). .. To verify transfection of pBTdel279, using plasmid DNA as the template, the target fragment was amplified using Qiagen Multiplex PCR kit (Qiagen China Co., Ltd., Shanghai, China) with the following primer sequences: 5′-TTGATATCGACCCCCGGGTCCGCCCGGAGCA-3′; and 5′-CTGAATTCGCTGCCTGAAACTCGCGCCGCGAG-3′, containing an Eco RV/ Eco RI restriction enzyme sites.

    Mass Spectrometry:

    Article Title: Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx
    Article Snippet: LC-MS data were obtained from an 1100 series HPLC (Agilent, Santa Clara, USA)-microTOF mass spectrometer (Bruker Daltonics) system, using electrospray ionization with a Cadenza CD-C18 column (2.0 i.d. .. Plasmid extraction was done according to Wizard® Plus SV Minipreps DNA Purification System (Promega, Tokyo, Japan).

    DNA Ligation:

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The PCR products obtained were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR products were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Construct:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: It consists of the human VLCAD sequence devoid of exon 3 by alternative splicing of the N-terminal region [VLCAD (ΔE x3)] inserted into a pET21a plasmid. pET21A-VLCAD (ΔE x3) was inserted by heat shock into competent XL1Blue E. coli (obtained by the CaCl2 method) and grown overnight at 37 °C. .. The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water. .. The VLCAD (ΔE x3) sequence was amplified by PCR, separated in an agarose gel and purified with the Promega Wizard® PCR Gel and PCR Clean Up kit.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx
    Article Snippet: The metagenomic library was constructed according to the protocol in the CopyControl™ Fosmid Libaray Production Kit with pCC1FOSTM vector (Epicentre Technologies, Madison, WI, USA). .. Plasmid extraction was done according to Wizard® Plus SV Minipreps DNA Purification System (Promega, Tokyo, Japan).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Incubation:

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: Briefly, 2 μg of extracted total RNA, 1 μL of dNTPs and 1 μL of 50 μM Oligo(dT)20 in a total 10 μL reaction volume was incubated at 65 °C for 5 min and placed on ice for 1 min. Then, 10 μL cDNA synthesis mix (2 μL 10x RT buffer, 4 μL 25 mM MgCl2 , 2 μL 0.1 M DTT, 1 μL RNaseOUT™ and 1 μL SuperScript® III RT) was added and RT reaction was performed at 50 °C for 50 min, terminated at 85 °C for 5 min and RNA was removed by incubating with 1 μL of 2 U/μL RNase H at 37 °C for 20 min. .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Expressing:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: Paragraph title: VLCAD (ΔE x3) expression in Escherichia coli and purification ... The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. Resultant PrxII-TG, C272A-TG, and Actb-TG transgenic founder mice were identified by PCR using HSA-specific primers (Supplementary Table ) and crossed with C57BL/10 mice to check for transgene transmission.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Once verified, the entry vector was recombined into the Gateway insect cell destination vector pDEST8 (pDEST8-N-FLAG-PRDX2) using LR Clonase II (Invitrogen) and subsequently expressed in Sf9 insect cells using the Bac-to-Bac system (Invitrogen; see Protein expression and purification). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. Resultant PrxII-TG, C272A-TG, and Actb-TG transgenic founder mice were identified by PCR using HSA-specific primers (Supplementary Table ) and crossed with C57BL/10 mice to check for transgene transmission.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Once verified, the entry vector was recombined into the Gateway insect cell destination vector pDEST8 (pDEST8-N-FLAG-PRDX2) using LR Clonase II (Invitrogen) and subsequently expressed in Sf9 insect cells using the Bac-to-Bac system (Invitrogen; see Protein expression and purification). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Western Blot:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. Resultant PrxII-TG, C272A-TG, and Actb-TG transgenic founder mice were identified by PCR using HSA-specific primers (Supplementary Table ) and crossed with C57BL/10 mice to check for transgene transmission.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. Resultant PrxII-TG, C272A-TG, and Actb-TG transgenic founder mice were identified by PCR using HSA-specific primers (Supplementary Table ) and crossed with C57BL/10 mice to check for transgene transmission.

    Transformation Assay:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
    Article Snippet: DH5α cells were transformed with the ligation product and plated on LB agar supplemented with ampicillin. .. Colonies were picked and cultured, and plasmids extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: Purified products were cloned using the pGEM®-T Vector System (Promega, Madison, WI, USA) and transformed into bacteria, following the manufacturer's instructions. .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Plasmids with transposons inserted were introduced into E. coli EC100D pir -116 competent cells, and transformed cells were selected on LB agar plates containing 100 μg/mL kanamycin. .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.).

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: Transformed E. coli cells carrying recombinant plasmid DNA were grown overnight in Luria Bertani (LB) broth (10 mL, Sigma Aldrich, USA) containing kanamycin (50 μg mL−1 , Invitrogen, USA) at 37 °C at 300 rpm constant shaking. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. One of the positive transformants, designated as FIP-pGEM-T_3, was used as a template for PCR cloning into the NheI and HindIII site of pET28a(+) expression vector.

    High Performance Liquid Chromatography:

    Article Title: Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx
    Article Snippet: LC-MS data were obtained from an 1100 series HPLC (Agilent, Santa Clara, USA)-microTOF mass spectrometer (Bruker Daltonics) system, using electrospray ionization with a Cadenza CD-C18 column (2.0 i.d. .. Plasmid extraction was done according to Wizard® Plus SV Minipreps DNA Purification System (Promega, Tokyo, Japan).

    Transfection:

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Plasmid pBCVP2G5-luc-NSN, pBTdel279, pCDNA3-Fas carrying the Fas gene and shuttle plasmid pDC316 were transfected into Escherichia coli JM109 competent cells (Promega Corporation, Madison, WI, USA) using Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h, according to the manufacturer's protocol. .. Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation).

    Sequencing:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: It consists of the human VLCAD sequence devoid of exon 3 by alternative splicing of the N-terminal region [VLCAD (ΔE x3)] inserted into a pET21a plasmid. pET21A-VLCAD (ΔE x3) was inserted by heat shock into competent XL1Blue E. coli (obtained by the CaCl2 method) and grown overnight at 37 °C. .. The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: To investigate whether sequence assembly would improve for plasmids if the main linear chromosome was removed prior to library construction, we used plasmid purification kits. .. : 27104); Wizard® Plus SV Minipreps DNA Purification System (Promega, cat.nr.

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega). .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Disrupted Intestinal Microbiota and Intestinal Inflammation in Children with Cystic Fibrosis and Its Restoration with Lactobacillus GG: A Randomised Clinical Trial
    Article Snippet: Paragraph title: Recovery of Amplicons and Sequencing ... The PCR products were purified using Wizard Plus® SV Minipreps-DNA Purification System (Promega) and were sequenced (BMR Genomics, Padova, Italy).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction. .. For proof-reading PCR, Phusion™ High-Fidelity DNA Polymerase (Finnzymes, Espoo, Finland) was used with 5x Phusion HF Buffer supplied.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. Bhd.

    Article Title: The Physiological Functions and Structural Determinants of Catalytic Bias in the [FeFe]-Hydrogenases CpI and CpII of Clostridium pasteurianum Strain W5
    Article Snippet: Paragraph title: Genome Sequencing ... Total genomic DNA of C. pasteurianum strain W5 was extracted using a Promega Wizard® Plus SV minipreps DNA purification system.

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation). .. To verify transfection of pBTdel279, using plasmid DNA as the template, the target fragment was amplified using Qiagen Multiplex PCR kit (Qiagen China Co., Ltd., Shanghai, China) with the following primer sequences: 5′-TTGATATCGACCCCCGGGTCCGCCCGGAGCA-3′; and 5′-CTGAATTCGCTGCCTGAAACTCGCGCCGCGAG-3′, containing an Eco RV/ Eco RI restriction enzyme sites.

    Ligation:

    Article Title: Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
    Article Snippet: DH5α cells were transformed with the ligation product and plated on LB agar supplemented with ampicillin. .. Colonies were picked and cultured, and plasmids extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. One of the positive transformants, designated as FIP-pGEM-T_3, was used as a template for PCR cloning into the NheI and HindIII site of pET28a(+) expression vector.

    Cell Culture:

    Article Title: Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
    Article Snippet: DH5α cells were transformed with the ligation product and plated on LB agar supplemented with ampicillin. .. Colonies were picked and cultured, and plasmids extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. Sequences of plasmids were confirmed by performing Sanger sequencing.

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Plasmids with transposons inserted were introduced into E. coli EC100D pir -116 competent cells, and transformed cells were selected on LB agar plates containing 100 μg/mL kanamycin. .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.). .. DNA sequencing was conducted using a 3130 capillary DNA sequencer (Applied Biosystems, Foster City, CA, United States) to determine the nucleotide sequences of the obtained plasmid.

    Generated:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The Actb-TG construct was generated via PCR using primers designed such that they amplified the Actg1-TG plasmid with ACTB-specific overhangs. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The Actb-TG construct was generated via PCR using primers designed such that they amplified the Actg1-TG plasmid with ACTB-specific overhangs. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: For each plasmid, 377 bp segment of rpoB gene containing the RRDR region was synthetically generated and then cloned into a 2507 bp pEX-K vector (Eurofins MWG Operon, Germany). .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions.

    CpG Methylation Assay:

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: Paragraph title: CpG methylation analysis by bisulfite sequencing ... Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: Paragraph title: CpG methylation analysis by bisulfite sequencing ... Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    DNA Sequencing:

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. Ligation mixture was transformed into BL21 strain E. coli cells and selected on Luria Bertani (LB) agar supplemented with 50 μg/mL kanamycin.

    Transmission Assay:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: SuperScript® III First-Strand Synthesis System for RT-PCR (Invitrogen, USA) was used for cDNA synthesis according to manufacturer’s instruction. .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Recombinant:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: To make recombinant FLAG-PrxII protein, pENTR/D-TOPO-PRDX2 was N-terminally FLAG-tagged (pENTR/D-TOPO-N-FLAG-PRDX2) via PCR using primers that amplified the entire plasmid with FLAG sequence overhangs. .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: To make recombinant FLAG-PrxII protein, pENTR/D-TOPO-PRDX2 was N-terminally FLAG-tagged (pENTR/D-TOPO-N-FLAG-PRDX2) via PCR using primers that amplified the entire plasmid with FLAG sequence overhangs. .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach
    Article Snippet: The PCR-amplified fragments with four additional bases (CACC) at the 5’-terminus for directional TOPO® cloning were then inserted into the pET101/D-TOPO vector (Thermo Fisher Scientific, Waltham, MA, USA) and introduced into E. coli TOP10, which was a host for the vector recommended by the supplier for efficient cloning. .. The recombinant plasmids were extracted from the E. coli culture using a Wizard plus SV Minipreps DNA Purification System (Promega, Fitchburg, WI, USA). .. The inserted sequences of the plasmids were analysed on an ABI 3730 DNA analyser (Thermo Fisher Scientific).

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: The bacterial cultures were harvested by centrifugation at 6000×g for 15 min at 4 °C. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions. .. The purified plasmid DNA samples were quantified and then stored at −20 °C.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. Bhd.

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Paragraph title: Construction of recombinant plasmid vectors carrying hTERT promoter and/or TSTA regulatory element and Fas gene ... Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation).

    DNA Extraction:

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: Paragraph title: DNA isolation, manipulation and amplification ... The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. Bhd.

    Nucleic Acid Electrophoresis:

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega). .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    DNA Purification:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: It consists of the human VLCAD sequence devoid of exon 3 by alternative splicing of the N-terminal region [VLCAD (ΔE x3)] inserted into a pET21a plasmid. pET21A-VLCAD (ΔE x3) was inserted by heat shock into competent XL1Blue E. coli (obtained by the CaCl2 method) and grown overnight at 37 °C. .. The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water. .. The VLCAD (ΔE x3) sequence was amplified by PCR, separated in an agarose gel and purified with the Promega Wizard® PCR Gel and PCR Clean Up kit.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr. .. : 27104); Wizard® Plus SV Minipreps DNA Purification System (Promega, cat.nr. .. : A1270)).

    Article Title: Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
    Article Snippet: DH5α cells were transformed with the ligation product and plated on LB agar supplemented with ampicillin. .. Colonies were picked and cultured, and plasmids extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. Sequences of plasmids were confirmed by performing Sanger sequencing.

    Article Title: Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx
    Article Snippet: DNA was extracted according to DNeasy® Mini Kit (QIAGEN, Tokyo, Japan). .. Plasmid extraction was done according to Wizard® Plus SV Minipreps DNA Purification System (Promega, Tokyo, Japan). .. DNA ligation was done according to DNA ligation kit ver.

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The PCR products obtained were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c). .. The promoter sequence details of immune checkpoints and PD-L1 genes are shown in Additional file : Figure S2 A-F.

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR products were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c). .. The promoter regions amplified for CpG methylation profile in this study were as previously described [ ].

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: Purified products were cloned using the pGEM®-T Vector System (Promega, Madison, WI, USA) and transformed into bacteria, following the manufacturer's instructions. .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega). .. Sequencing was performed by GATC Biotech (Konstanz, Germany).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The PrxII cDNA was inserted in-frame between the VP1 intron and the first SV40 polyadenylation sequence (pDEST8-HSA-VP1-PRDX2-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). .. The DNA fragment from the HSA promoter through the polyadenylation sequence was restriction digested to linearize the transgene, gel purified via the QIAEX II Gel Extraction Kit (Qiagen), and sent to the Murine Genetics Core at The Scripps Research Institute for pronuclear microinjection into fertilized C57BL/6 zygotes, which were then implanted into pseudo-pregnant female mice.

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Plasmids with transposons inserted were introduced into E. coli EC100D pir -116 competent cells, and transformed cells were selected on LB agar plates containing 100 μg/mL kanamycin. .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.). .. DNA sequencing was conducted using a 3130 capillary DNA sequencer (Applied Biosystems, Foster City, CA, United States) to determine the nucleotide sequences of the obtained plasmid.

    Article Title: Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach
    Article Snippet: The PCR-amplified fragments with four additional bases (CACC) at the 5’-terminus for directional TOPO® cloning were then inserted into the pET101/D-TOPO vector (Thermo Fisher Scientific, Waltham, MA, USA) and introduced into E. coli TOP10, which was a host for the vector recommended by the supplier for efficient cloning. .. The recombinant plasmids were extracted from the E. coli culture using a Wizard plus SV Minipreps DNA Purification System (Promega, Fitchburg, WI, USA). .. The inserted sequences of the plasmids were analysed on an ABI 3730 DNA analyser (Thermo Fisher Scientific).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: The same kit was also used for clean-up of the pJQ200mp18 vector following the restriction enzyme digestion. .. The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction. .. For DNA amplification, 2X PCR Master Mix (Promega, Sunnyvale, CA, USA) was used.

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: The bacterial cultures were harvested by centrifugation at 6000×g for 15 min at 4 °C. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions. .. The purified plasmid DNA samples were quantified and then stored at −20 °C.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. Bhd.

    Article Title: The Physiological Functions and Structural Determinants of Catalytic Bias in the [FeFe]-Hydrogenases CpI and CpII of Clostridium pasteurianum Strain W5
    Article Snippet: An aliquot (1 ml) of the culture was spun down at 14,000 × g at room temperature before extracting DNA. .. Total genomic DNA of C. pasteurianum strain W5 was extracted using a Promega Wizard® Plus SV minipreps DNA purification system. .. The concentration (220 ng/μL) was determined by a NanoDrop 1000 Spectrophotometer (Abs260/280 = 2.04).

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Plasmid pBCVP2G5-luc-NSN, pBTdel279, pCDNA3-Fas carrying the Fas gene and shuttle plasmid pDC316 were transfected into Escherichia coli JM109 competent cells (Promega Corporation, Madison, WI, USA) using Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h, according to the manufacturer's protocol. .. Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation). .. To verify transfection of pBTdel279, using plasmid DNA as the template, the target fragment was amplified using Qiagen Multiplex PCR kit (Qiagen China Co., Ltd., Shanghai, China) with the following primer sequences: 5′-TTGATATCGACCCCCGGGTCCGCCCGGAGCA-3′; and 5′-CTGAATTCGCTGCCTGAAACTCGCGCCGCGAG-3′, containing an Eco RV/ Eco RI restriction enzyme sites.

    Mutagenesis:

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: These plasmids contained four of the most predominant mutations (S531L, S531W, H526Y, or D516V) and a fifth wild-type (WT) plasmid with no mutation in the 81 bp hot-spot region. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions.

    Isolation:

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Paragraph title: Isolation and Characterization of pKPAL3 from K. palustris IPUFS-1 ... Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.).

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: The bacterial cultures were harvested by centrifugation at 6000×g for 15 min at 4 °C. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions. .. The purified plasmid DNA samples were quantified and then stored at −20 °C.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: Paragraph title: Isolation and cloning of the FIP-Lrh cDNA ... White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Size-exclusion Chromatography:

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: PCR was performed in the Mastercycler® Thermal Cycler (Eppendorf) with initial denaturation at 95 °C for 5 min; 25 cycles of denaturation at 95 °C for 30 sec, annealing at 60 °C for 30 sec, extension at 72 °C for 1 min; and a final extension at 72 °C for 5 min. .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Purification:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: It consists of the human VLCAD sequence devoid of exon 3 by alternative splicing of the N-terminal region [VLCAD (ΔE x3)] inserted into a pET21a plasmid. pET21A-VLCAD (ΔE x3) was inserted by heat shock into competent XL1Blue E. coli (obtained by the CaCl2 method) and grown overnight at 37 °C. .. The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water. .. The VLCAD (ΔE x3) sequence was amplified by PCR, separated in an agarose gel and purified with the Promega Wizard® PCR Gel and PCR Clean Up kit.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Once verified, the entry vector was recombined into the Gateway insect cell destination vector pDEST8 (pDEST8-N-FLAG-PRDX2) using LR Clonase II (Invitrogen) and subsequently expressed in Sf9 insect cells using the Bac-to-Bac system (Invitrogen; see Protein expression and purification). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: For an initial assessment of suitability of plasmid purification kits for separation of the Borrelia main chromosome from plasmids, three different plasmid purification kits were run in duplicate, two from Qiagen and one from Promega (QIAGEN® Plasmid Mini kit (QIAGEN®, cat.nr. .. : 27104); Wizard® Plus SV Minipreps DNA Purification System (Promega, cat.nr.

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The PCR products obtained were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c). .. The promoter sequence details of immune checkpoints and PD-L1 genes are shown in Additional file : Figure S2 A-F.

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR products were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c). .. The promoter regions amplified for CpG methylation profile in this study were as previously described [ ].

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: Purified products were cloned using the pGEM®-T Vector System (Promega, Madison, WI, USA) and transformed into bacteria, following the manufacturer's instructions. .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Once verified, the entry vector was recombined into the Gateway insect cell destination vector pDEST8 (pDEST8-N-FLAG-PRDX2) using LR Clonase II (Invitrogen) and subsequently expressed in Sf9 insect cells using the Bac-to-Bac system (Invitrogen; see Protein expression and purification). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Transposons were inserted into the purified plasmid with the EZ-Tn5TM < R6Kγori /KAN-2: kanamycin resistant > Insertion Kit (AR Brown, Tokyo, Japan) according to the manufacture’s protocol. .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.).

    Article Title: Disrupted Intestinal Microbiota and Intestinal Inflammation in Children with Cystic Fibrosis and Its Restoration with Lactobacillus GG: A Randomised Clinical Trial
    Article Snippet: Bands were cut from DGGE gels, eluted at 4°C for 24 h, and re-amplified according to the PCR conditions described above. .. The PCR products were purified using Wizard Plus® SV Minipreps-DNA Purification System (Promega) and were sequenced (BMR Genomics, Padova, Italy). .. Sequences obtained were analyzed using NCBI BLAST (Basic Local Alignment Search Tool) and Ribosomal Database Project (RDP).

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: The bacterial cultures were harvested by centrifugation at 6000×g for 15 min at 4 °C. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions. .. The purified plasmid DNA samples were quantified and then stored at −20 °C.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Plasmid pBCVP2G5-luc-NSN, pBTdel279, pCDNA3-Fas carrying the Fas gene and shuttle plasmid pDC316 were transfected into Escherichia coli JM109 competent cells (Promega Corporation, Madison, WI, USA) using Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) for 48 h, according to the manufacturer's protocol. .. Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation). .. To verify transfection of pBTdel279, using plasmid DNA as the template, the target fragment was amplified using Qiagen Multiplex PCR kit (Qiagen China Co., Ltd., Shanghai, China) with the following primer sequences: 5′-TTGATATCGACCCCCGGGTCCGCCCGGAGCA-3′; and 5′-CTGAATTCGCTGCCTGAAACTCGCGCCGCGAG-3′, containing an Eco RV/ Eco RI restriction enzyme sites.

    Polymerase Chain Reaction:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water. .. The VLCAD (ΔE x3) sequence was amplified by PCR, separated in an agarose gel and purified with the Promega Wizard® PCR Gel and PCR Clean Up kit.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The PCR products obtained were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR products were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega). .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The linear PCR product was circularized via the addition of T4 polynucleotide kinase and T4 DNA ligase (New England Biolabs) and sequence verified. .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Plasmids were then extracted from agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System (Promega Corp.). .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.).

    Article Title: Disrupted Intestinal Microbiota and Intestinal Inflammation in Children with Cystic Fibrosis and Its Restoration with Lactobacillus GG: A Randomised Clinical Trial
    Article Snippet: Bands were cut from DGGE gels, eluted at 4°C for 24 h, and re-amplified according to the PCR conditions described above. .. The PCR products were purified using Wizard Plus® SV Minipreps-DNA Purification System (Promega) and were sequenced (BMR Genomics, Padova, Italy). .. Sequences obtained were analyzed using NCBI BLAST (Basic Local Alignment Search Tool) and Ribosomal Database Project (RDP).

    Article Title: Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach
    Article Snippet: The PCR-amplified fragments with four additional bases (CACC) at the 5’-terminus for directional TOPO® cloning were then inserted into the pET101/D-TOPO vector (Thermo Fisher Scientific, Waltham, MA, USA) and introduced into E. coli TOP10, which was a host for the vector recommended by the supplier for efficient cloning. .. The recombinant plasmids were extracted from the E. coli culture using a Wizard plus SV Minipreps DNA Purification System (Promega, Fitchburg, WI, USA).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: After separation of PCR amplified products by agarose gel electrophoresis, DNA fragments were excised from the gel and extracted using the Wizard SV Gel and PCR Clean-up system kit (Promega, Sunnyvale, CA, USA) following the manufacturer’s protocol. .. The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn.

    CRISPR:

    Article Title: Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
    Article Snippet: Paragraph title: CRISPR/Cas9 enhancer deletions ... Colonies were picked and cultured, and plasmids extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: : 12123); QIAprep® Spin Miniprep kit (QIAGEN®, cat.nr. .. : 27104); Wizard® Plus SV Minipreps DNA Purification System (Promega, cat.nr. .. : A1270)).

    Mouse Assay:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Paragraph title: Cloning and generation of transgenic mice ... Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Plasmid Preparation:

    Article Title: H2O2 release from the very long chain acyl-CoA dehydrogenase
    Article Snippet: It consists of the human VLCAD sequence devoid of exon 3 by alternative splicing of the N-terminal region [VLCAD (ΔE x3)] inserted into a pET21a plasmid. pET21A-VLCAD (ΔE x3) was inserted by heat shock into competent XL1Blue E. coli (obtained by the CaCl2 method) and grown overnight at 37 °C. .. The expanded construct was then purified with the Promega Wizard® Plus SV Minipreps DNA Purification System and eluted in nuclease-free water.

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The Actb-TG construct was generated via PCR using primers designed such that they amplified the Actg1-TG plasmid with ACTB-specific overhangs. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: To generate the PrxII-TG transgene, pENTR/D-TOPO-PRDX2 was recombined into a pDEST8 destination vector already containing the HSA promoter followed by the VP1 intron and tandem SV40 polyadenylation sequences (pDEST8-HSA-VP1-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi
    Article Snippet: Paragraph title: DNA purification, plasmid enrichment and library construction ... : 27104); Wizard® Plus SV Minipreps DNA Purification System (Promega, cat.nr.

    Article Title: Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx
    Article Snippet: DNA was extracted according to DNeasy® Mini Kit (QIAGEN, Tokyo, Japan). .. Plasmid extraction was done according to Wizard® Plus SV Minipreps DNA Purification System (Promega, Tokyo, Japan). .. DNA ligation was done according to DNA ligation kit ver.

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The PCR products obtained were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR products were cloned into the pGemT-easy vector (Promega, Madison, USA) using DNA Ligation Kit, Mighty Mix (TaKaRa Bio). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: Purified products were cloned using the pGEM®-T Vector System (Promega, Madison, WI, USA) and transformed into bacteria, following the manufacturer's instructions. .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: The Actb-TG construct was generated via PCR using primers designed such that they amplified the Actg1-TG plasmid with ACTB-specific overhangs. .. Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: To generate the PrxII-TG transgene, pENTR/D-TOPO-PRDX2 was recombined into a pDEST8 destination vector already containing the HSA promoter followed by the VP1 intron and tandem SV40 polyadenylation sequences (pDEST8-HSA-VP1-SV40-SV40). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Transposons were inserted into the purified plasmid with the EZ-Tn5TM < R6Kγori /KAN-2: kanamycin resistant > Insertion Kit (AR Brown, Tokyo, Japan) according to the manufacture’s protocol. .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.).

    Article Title: Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach
    Article Snippet: The PCR-amplified fragments with four additional bases (CACC) at the 5’-terminus for directional TOPO® cloning were then inserted into the pET101/D-TOPO vector (Thermo Fisher Scientific, Waltham, MA, USA) and introduced into E. coli TOP10, which was a host for the vector recommended by the supplier for efficient cloning. .. The recombinant plasmids were extracted from the E. coli culture using a Wizard plus SV Minipreps DNA Purification System (Promega, Fitchburg, WI, USA).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: The same kit was also used for clean-up of the pJQ200mp18 vector following the restriction enzyme digestion. .. The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction.

    Article Title: Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
    Article Snippet: The bacterial cultures were harvested by centrifugation at 6000×g for 15 min at 4 °C. .. The supernatant was removed and the cell pellets consisting of the recombinant plasmid were isolated and purified with Wizard® Plus SV Minipreps DNA Purification System (Promega, USA) according to the manufacturer’s instructions. .. The purified plasmid DNA samples were quantified and then stored at −20 °C.

    Article Title: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis
    Article Snippet: The PCR product was gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and cloned using the pGEM® -T Easy Vector System (Promega, USA). .. White colonies were picked for plasmid DNA extraction using the Wizard® Plus SV Minipreps DNA Purification System (Promega) and recombinant plasmids from four positive transformants were sent for sequencing of both DNA strands at MyTAGC Bioscience Sdn. .. Bhd.

    Article Title: Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
    Article Snippet: Paragraph title: Construction of recombinant plasmid vectors carrying hTERT promoter and/or TSTA regulatory element and Fas gene ... Following amplification, bacterial plasmids were extracted and purified using the Wizard Plus SV Minipreps DNA Purification system (Promega Corporation).

    Software:

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: PCR primers were designed using MethPrimer software ( http://www.urogene.org/methprimer/index1.html ), all are listed in Additional file : Table S1b. .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: PCR primers were designed using MethPrimer software ( http://www.urogene.org/methprimer/index1.html ). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega). .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Disrupted Intestinal Microbiota and Intestinal Inflammation in Children with Cystic Fibrosis and Its Restoration with Lactobacillus GG: A Randomised Clinical Trial
    Article Snippet: Bands were cut from DGGE gels, eluted at 4°C for 24 h, and re-amplified according to the PCR conditions described above. .. The PCR products were purified using Wizard Plus® SV Minipreps-DNA Purification System (Promega) and were sequenced (BMR Genomics, Padova, Italy).

    Agarose Gel Electrophoresis:

    Article Title: Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice
    Article Snippet: From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega). .. From 5 to 15 bacterial colonies of each sample were grown in culture overnight and the plasmids were extracted using the Wizard® Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Development of a Novel Escherichia coli–Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide
    Article Snippet: Plasmids were then extracted from agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System (Promega Corp.). .. Selected transformants were cultured in 4 mL of LB liquid medium containing 100 μg/mL kanamycin, and the plasmids were prepared using the Wizard Plus SV Minipreps DNA purification system (Promega Corp.).

    Article Title: Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein)
    Article Snippet: After separation of PCR amplified products by agarose gel electrophoresis, DNA fragments were excised from the gel and extracted using the Wizard SV Gel and PCR Clean-up system kit (Promega, Sunnyvale, CA, USA) following the manufacturer’s protocol. .. The Wizard® Plus SV Minipreps DNA Purification System (Promega, Sunnyvale, CA, USA) was used to isolate plasmids from microorganisms according to the manufacturer’s instruction.

    Transgenic Assay:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Paragraph title: Cloning and generation of transgenic mice ... Both constructs were transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    DNA Methylation Assay:

    Article Title: DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer
    Article Snippet: The genomic DNA was extracted from tumor and normal tissues as described before and treated with bisulfite using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA, United States). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced with M13-reverse/forward primers (Additional file : Table S1c).

    Article Title: DNA methylation and repressive histones in the promoters of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9 genes in human colorectal cancer
    Article Snippet: Briefly, genomic DNA was extracted from NT and TT, and bisulfite treatment was performed using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA). .. Ten individual clones from each sample were purified using Wizard® Plus SV Minipreps DNA Purification System (Promega) and sequenced using M13-reverse/forward primers (Additional file : Table S1c).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx
    Article Snippet: LC-MS data were obtained from an 1100 series HPLC (Agilent, Santa Clara, USA)-microTOF mass spectrometer (Bruker Daltonics) system, using electrospray ionization with a Cadenza CD-C18 column (2.0 i.d. .. Plasmid extraction was done according to Wizard® Plus SV Minipreps DNA Purification System (Promega, Tokyo, Japan).

    BAC Assay:

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Once verified, the entry vector was recombined into the Gateway insect cell destination vector pDEST8 (pDEST8-N-FLAG-PRDX2) using LR Clonase II (Invitrogen) and subsequently expressed in Sf9 insect cells using the Bac-to-Bac system (Invitrogen; see Protein expression and purification). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

    Article Title: Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle
    Article Snippet: Once verified, the entry vector was recombined into the Gateway insect cell destination vector pDEST8 (pDEST8-N-FLAG-PRDX2) using LR Clonase II (Invitrogen) and subsequently expressed in Sf9 insect cells using the Bac-to-Bac system (Invitrogen; see Protein expression and purification). .. The construct was transformed into DH5α bacteria cells and extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega).

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    Promega wizard plus sv miniprep dna purification system
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