wizard pcr cleanup kit  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Wizard SV Gel and PCR Clean Up System
    Description:
    Purifies DNA fragments from gel slices or PCR in 15 minutes
    Catalog Number:
    a9281
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Nucleic Acid Extraction Clean up and Concentration
    Buy from Supplier


    Structured Review

    Promega wizard pcr cleanup kit
    Purifies DNA fragments from gel slices or PCR in 15 minutes
    https://www.bioz.com/result/wizard pcr cleanup kit/product/Promega
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    wizard pcr cleanup kit - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega); cloning was with pGEM-T Easy Vector System (Promega). .. Sequence-reads were assembled into contigs with Sequencher 5.0 (Gene Codes); the parameters for assembly were minimum match 90% and minimum overlap 100 bp. tRNA genes were identified with tRNA-Scan ( ) and ARWEN ( ).

    Amplification:

    Article Title: SRY Induced TCF21 Genome-Wide Targets and Cascade of bHLH Factors During Sertoli Cell Differentiation and Male Sex Determination in Rats 1
    Article Snippet: .. Pooled whole-genome amplified DNA was purified by using the Wizard SV40 PCR Clean-up System (A9281; Promega). .. Purified DNA was checked on the gel and sent to Roche NimbleGen for ChIP-chip hybridization, and a three-plex promoter array was used for competitive hybridizations.

    Agarose Gel Electrophoresis:

    Article Title: CTNND1 755 T > G Promoter Polymorphism and Risk of Pancreatic Carcinoma in Chinese
    Article Snippet: .. The PCR fragments were recovered from agarose gel followed by purification with a DNA clean‐up kit (Wizard SV Gel and PCR Clean‐up System, Promega). .. DNA sequences of the PCR products were determined using the PCR sense primer with an Applied Biosystems model 377 sequencer (PE Applied Biosystems, Warrington, UK).

    Mutagenesis:

    Article Title: Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)
    Article Snippet: .. Genomic DNA of GAS osKaR mutant (see Basic Protocol 1) Primers oPCR1, Deg3, Anchor1, Deg4 and Anchor2 (see recipe) Reagents and equipment for PCR Gel and PCR Clean-Up System kit (Wizard SV, Promega Cat. No. A9282) .. 1 Set up the first PCR (AP-PCR #1) using 1 μl of genomic DNA, 1 μl of primers oPCR1 and Deg3 (10 μM each) using Taq .

    Purification:

    Article Title: SRY Induced TCF21 Genome-Wide Targets and Cascade of bHLH Factors During Sertoli Cell Differentiation and Male Sex Determination in Rats 1
    Article Snippet: .. Pooled whole-genome amplified DNA was purified by using the Wizard SV40 PCR Clean-up System (A9281; Promega). .. Purified DNA was checked on the gel and sent to Roche NimbleGen for ChIP-chip hybridization, and a three-plex promoter array was used for competitive hybridizations.

    Article Title: Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega); cloning was with pGEM-T Easy Vector System (Promega). .. Sequence-reads were assembled into contigs with Sequencher 5.0 (Gene Codes); the parameters for assembly were minimum match 90% and minimum overlap 100 bp. tRNA genes were identified with tRNA-Scan ( ) and ARWEN ( ).

    Article Title: CTNND1 755 T > G Promoter Polymorphism and Risk of Pancreatic Carcinoma in Chinese
    Article Snippet: .. The PCR fragments were recovered from agarose gel followed by purification with a DNA clean‐up kit (Wizard SV Gel and PCR Clean‐up System, Promega). .. DNA sequences of the PCR products were determined using the PCR sense primer with an Applied Biosystems model 377 sequencer (PE Applied Biosystems, Warrington, UK).

    Article Title: Substantial Variation in the Extent of Mitochondrial Genome Fragmentation among Blood-Sucking Lice of Mammals
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega). .. Next-Generation Sequencing of the Coding Regions of mt Minichromosomes Purified PCR amplicons generated above with primers PLF1 and PLR from the coding regions of the mt minichromosomes of the domestic pig louse and the wild pig louse were sequenced initially with Roche GS FLX (454) platform at the AGRF and then with Illumina Hiseq 2000 platform at the Beijing Genomics Institute (BGI) for deeper coverages.

    Article Title: Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points
    Article Snippet: .. DNA was purified from proteinase K–treated samples using a DNA purification kit following the manufacturer instructions (A9282; Promega) and was subsequently analyzed either by running a 2% low melting agarose (APEX) gel or by an Agilent Technologies 2100 Bioanalyzer by using the DNA 1000 kit. .. The Bioanalyzer determines the quantity of DNA on the basis of fluorescence intensity.

    Real-time Polymerase Chain Reaction:

    Article Title: IGF2BP1 promotes SRF-dependent transcription in cancer in a m6A- and miRNA-dependent manner
    Article Snippet: .. DNA was finally eluted using the WIZARD® SV Gel & PCR Clean-Up System (Promega A9281) according to the manufacturer’s protocol and analyzed by quantitative real-time PCR (qPCR). .. RNA immunoprecipitation (RIP) and quantitative RT-PCR analyses were performed essentially as recently described ( ).

    Polymerase Chain Reaction:

    Article Title: IGF2BP1 promotes SRF-dependent transcription in cancer in a m6A- and miRNA-dependent manner
    Article Snippet: .. DNA was finally eluted using the WIZARD® SV Gel & PCR Clean-Up System (Promega A9281) according to the manufacturer’s protocol and analyzed by quantitative real-time PCR (qPCR). .. RNA immunoprecipitation (RIP) and quantitative RT-PCR analyses were performed essentially as recently described ( ).

    Article Title: SRY Induced TCF21 Genome-Wide Targets and Cascade of bHLH Factors During Sertoli Cell Differentiation and Male Sex Determination in Rats 1
    Article Snippet: .. Pooled whole-genome amplified DNA was purified by using the Wizard SV40 PCR Clean-up System (A9281; Promega). .. Purified DNA was checked on the gel and sent to Roche NimbleGen for ChIP-chip hybridization, and a three-plex promoter array was used for competitive hybridizations.

    Article Title: Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega); cloning was with pGEM-T Easy Vector System (Promega). .. Sequence-reads were assembled into contigs with Sequencher 5.0 (Gene Codes); the parameters for assembly were minimum match 90% and minimum overlap 100 bp. tRNA genes were identified with tRNA-Scan ( ) and ARWEN ( ).

    Article Title: CTNND1 755 T > G Promoter Polymorphism and Risk of Pancreatic Carcinoma in Chinese
    Article Snippet: .. The PCR fragments were recovered from agarose gel followed by purification with a DNA clean‐up kit (Wizard SV Gel and PCR Clean‐up System, Promega). .. DNA sequences of the PCR products were determined using the PCR sense primer with an Applied Biosystems model 377 sequencer (PE Applied Biosystems, Warrington, UK).

    Article Title: Substantial Variation in the Extent of Mitochondrial Genome Fragmentation among Blood-Sucking Lice of Mammals
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega). .. Next-Generation Sequencing of the Coding Regions of mt Minichromosomes Purified PCR amplicons generated above with primers PLF1 and PLR from the coding regions of the mt minichromosomes of the domestic pig louse and the wild pig louse were sequenced initially with Roche GS FLX (454) platform at the AGRF and then with Illumina Hiseq 2000 platform at the Beijing Genomics Institute (BGI) for deeper coverages.

    Article Title: Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)
    Article Snippet: .. Genomic DNA of GAS osKaR mutant (see Basic Protocol 1) Primers oPCR1, Deg3, Anchor1, Deg4 and Anchor2 (see recipe) Reagents and equipment for PCR Gel and PCR Clean-Up System kit (Wizard SV, Promega Cat. No. A9282) .. 1 Set up the first PCR (AP-PCR #1) using 1 μl of genomic DNA, 1 μl of primers oPCR1 and Deg3 (10 μM each) using Taq .

    DNA Purification:

    Article Title: Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points
    Article Snippet: .. DNA was purified from proteinase K–treated samples using a DNA purification kit following the manufacturer instructions (A9282; Promega) and was subsequently analyzed either by running a 2% low melting agarose (APEX) gel or by an Agilent Technologies 2100 Bioanalyzer by using the DNA 1000 kit. .. The Bioanalyzer determines the quantity of DNA on the basis of fluorescence intensity.

    Sequencing:

    Article Title: Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega); cloning was with pGEM-T Easy Vector System (Promega). .. Sequence-reads were assembled into contigs with Sequencher 5.0 (Gene Codes); the parameters for assembly were minimum match 90% and minimum overlap 100 bp. tRNA genes were identified with tRNA-Scan ( ) and ARWEN ( ).

    Article Title: Substantial Variation in the Extent of Mitochondrial Genome Fragmentation among Blood-Sucking Lice of Mammals
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega). .. Next-Generation Sequencing of the Coding Regions of mt Minichromosomes Purified PCR amplicons generated above with primers PLF1 and PLR from the coding regions of the mt minichromosomes of the domestic pig louse and the wild pig louse were sequenced initially with Roche GS FLX (454) platform at the AGRF and then with Illumina Hiseq 2000 platform at the Beijing Genomics Institute (BGI) for deeper coverages.

    Plasmid Preparation:

    Article Title: Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans
    Article Snippet: .. PCR amplicons used for sequencing were purified with Wizard SV Gel/PCR Clean-up System (Promega); cloning was with pGEM-T Easy Vector System (Promega). .. Sequence-reads were assembled into contigs with Sequencher 5.0 (Gene Codes); the parameters for assembly were minimum match 90% and minimum overlap 100 bp. tRNA genes were identified with tRNA-Scan ( ) and ARWEN ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Promega wizard pcr cleanup kit
    Wizard Pcr Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard pcr cleanup kit/product/Promega
    Average 91 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    wizard pcr cleanup kit - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    Promega pcr cleanup kit
    Pcr Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr cleanup kit/product/Promega
    Average 93 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    pcr cleanup kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Promega wizard sv40 pcr cleanup kit
    Wizard Sv40 Pcr Cleanup Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard sv40 pcr cleanup kit/product/Promega
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    wizard sv40 pcr cleanup kit - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results