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Promega wizard pcr clean up minicolumns
Wizard Pcr Clean Up Minicolumns, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard pcr clean up minicolumns/product/Promega
Average 87 stars, based on 2 article reviews
Price from $9.99 to $1999.99
wizard pcr clean up minicolumns - by Bioz Stars, 2020-04
87/100 stars

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Clone Assay:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The amplified products were pooled and purified with Wizard® PCR Clean-Up minicolumns (Promega), followed by cloning into the pGEM®-T Easy vector (Promega) according to the manufacturer's instructions. .. After transformation into E. coli JM109 competent cells, the orientation of the inserted 16S rRNA gene of each resulting clone was determined by PCR re-amplification using 63F and M13 reverse primers.

Amplification:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The amplified products were pooled and purified with Wizard® PCR Clean-Up minicolumns (Promega), followed by cloning into the pGEM®-T Easy vector (Promega) according to the manufacturer's instructions. .. After transformation into E. coli JM109 competent cells, the orientation of the inserted 16S rRNA gene of each resulting clone was determined by PCR re-amplification using 63F and M13 reverse primers.

Purification:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The PCR products were purified with Wizard® PCR Clean-Up minicolumns (Promega), and the recovered 16S rDNA gene fragments were sequenced (Eurofins MWG Operon, Ebersberg, Germany). .. T-RFLP analysis

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The amplified products were pooled and purified with Wizard® PCR Clean-Up minicolumns (Promega), followed by cloning into the pGEM®-T Easy vector (Promega) according to the manufacturer's instructions. .. After transformation into E. coli JM109 competent cells, the orientation of the inserted 16S rRNA gene of each resulting clone was determined by PCR re-amplification using 63F and M13 reverse primers.

Electrophoresis:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: Paragraph title: Electrophoresis conditions and DGGE band analysis ... The PCR products were purified with Wizard® PCR Clean-Up minicolumns (Promega), and the recovered 16S rDNA gene fragments were sequenced (Eurofins MWG Operon, Ebersberg, Germany).

Polymerase Chain Reaction:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The PCR products were purified with Wizard® PCR Clean-Up minicolumns (Promega), and the recovered 16S rDNA gene fragments were sequenced (Eurofins MWG Operon, Ebersberg, Germany). .. T-RFLP analysis

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The amplified products were pooled and purified with Wizard® PCR Clean-Up minicolumns (Promega), followed by cloning into the pGEM®-T Easy vector (Promega) according to the manufacturer's instructions. .. After transformation into E. coli JM109 competent cells, the orientation of the inserted 16S rRNA gene of each resulting clone was determined by PCR re-amplification using 63F and M13 reverse primers.

Staining:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: Electrophoresis was performed for 17 h at 60 °C at a constant voltage of 80 V. The gels were stained with GelStar® (Cambrex BioScience, East Rutherford, NJ, USA) in 1 X TAE and visualized under UV light with a Gel Doc 2000® image system (Bio-Rad). .. The PCR products were purified with Wizard® PCR Clean-Up minicolumns (Promega), and the recovered 16S rDNA gene fragments were sequenced (Eurofins MWG Operon, Ebersberg, Germany).

Transformation Assay:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: The amplified products were pooled and purified with Wizard® PCR Clean-Up minicolumns (Promega), followed by cloning into the pGEM®-T Easy vector (Promega) according to the manufacturer's instructions. .. After transformation into E. coli JM109 competent cells, the orientation of the inserted 16S rRNA gene of each resulting clone was determined by PCR re-amplification using 63F and M13 reverse primers.

Denaturing Gradient Gel Electrophoresis:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: Paragraph title: Electrophoresis conditions and DGGE band analysis ... The PCR products were purified with Wizard® PCR Clean-Up minicolumns (Promega), and the recovered 16S rDNA gene fragments were sequenced (Eurofins MWG Operon, Ebersberg, Germany).

Plasmid Preparation:

Article Title: Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea
Article Snippet: .. The amplified products were pooled and purified with Wizard® PCR Clean-Up minicolumns (Promega), followed by cloning into the pGEM®-T Easy vector (Promega) according to the manufacturer's instructions. .. After transformation into E. coli JM109 competent cells, the orientation of the inserted 16S rRNA gene of each resulting clone was determined by PCR re-amplification using 63F and M13 reverse primers.

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  • 87
    Promega wizard pcr clean up minicolumns
    Wizard Pcr Clean Up Minicolumns, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard pcr clean up minicolumns/product/Promega
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    wizard pcr clean up minicolumns - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    99
    Promega wizard pcr preps dna purification system
    Wizard Pcr Preps Dna Purification System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard pcr preps dna purification system/product/Promega
    Average 99 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    wizard pcr preps dna purification system - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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