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wild type a baumannii strain atcc 17978 wt  (ATCC)


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    ATCC wild type a baumannii strain atcc 17978 wt
    Wild Type A Baumannii Strain Atcc 17978 Wt, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wild type a baumannii strain atcc 17978 wt  (ATCC)
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    ATCC wild type a baumannii strain atcc 17978 wt
    Wild Type A Baumannii Strain Atcc 17978 Wt, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a baumannii wild type wt strain atcc 17978  (ATCC)
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    ATCC a baumannii wild type wt strain atcc 17978
    A Baumannii Wild Type Wt Strain Atcc 17978, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wild type wt a baumannii strain atcc 17978  (ATCC)
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    ATCC wild type wt a baumannii strain atcc 17978
    Bacterial strains and plasmids used in this work.
    Wild Type Wt A Baumannii Strain Atcc 17978, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type wt a baumannii strain atcc 17978/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    wild type wt a baumannii atcc 17978 strain  (ATCC)
    86
    ATCC wild type wt a baumannii atcc 17978 strain
    Identification of DksA in A. baumannii . (a) Multiple alignments of translated sequences of A1S_0248 with DksA of other bacterial species. The gray highlight indicates identical amino acids with E. coli DksA. The bold characters indicate >50% consensus amino acids among the tested bacterial species. Sequence conservation is presented as the height of colored bars. The accession number of DksA proteins is ABO10723 in A. baumannii ATCC 17978, VWQ00927 in E. coli , CDO15944 in Klebsiella pneumoniae , WP_058139749 in Pseudomonas aeruginosa , KIS47657 in Burkholderia cepacia , RTY79203 in Staphylococcus aureus , WP_047919748 in Neisseria gonorrheae , WP_148842572 in Streptococcus pyogenes , SGC70216 in Mycobacterium tuberculosis , and VTQ30573 in Streptococcus pneumoniae . (b) Sequence logo of DksA between E. coli and A. baumannii <t>ATCC</t> <t>17978</t> using the WebLogo 3. The red boxes are essential amino acids for RNAP binding and DksA activity in E. coli . The number is based on the amino acid sequences of E. coli DksA. Furthermore, 151 amino acids of E. coli DksA (the first number in parenthesis) are compared with the translated amino acid sequences of A1S_0248 of A. baumannii ATCC 17978 (the second number in parenthesis) in the boxes as follows: D (74, 101); A (76, 103); R (91, 118); R (125, 152); A (128, 155); I (136, 163); E (143, 170); I-M (144–149, 171–176). (c) The predicted three-dimensional structure of proteins encoded by A1S_0248 using Swiss-MODEL. Red and green colors indicate the amino acids for the RNAP binding and DksA activity, respectively
    Wild Type Wt A Baumannii Atcc 17978 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type wt a baumannii atcc 17978 strain/product/ATCC
    Average 86 stars, based on 1 article reviews
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    wild type a baumannii strain 381 atcc 17978 wt  (ATCC)
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    ATCC wild type a baumannii strain 381 atcc 17978 wt
    Identification of DksA in A. baumannii . (a) Multiple alignments of translated sequences of A1S_0248 with DksA of other bacterial species. The gray highlight indicates identical amino acids with E. coli DksA. The bold characters indicate >50% consensus amino acids among the tested bacterial species. Sequence conservation is presented as the height of colored bars. The accession number of DksA proteins is ABO10723 in A. baumannii ATCC 17978, VWQ00927 in E. coli , CDO15944 in Klebsiella pneumoniae , WP_058139749 in Pseudomonas aeruginosa , KIS47657 in Burkholderia cepacia , RTY79203 in Staphylococcus aureus , WP_047919748 in Neisseria gonorrheae , WP_148842572 in Streptococcus pyogenes , SGC70216 in Mycobacterium tuberculosis , and VTQ30573 in Streptococcus pneumoniae . (b) Sequence logo of DksA between E. coli and A. baumannii <t>ATCC</t> <t>17978</t> using the WebLogo 3. The red boxes are essential amino acids for RNAP binding and DksA activity in E. coli . The number is based on the amino acid sequences of E. coli DksA. Furthermore, 151 amino acids of E. coli DksA (the first number in parenthesis) are compared with the translated amino acid sequences of A1S_0248 of A. baumannii ATCC 17978 (the second number in parenthesis) in the boxes as follows: D (74, 101); A (76, 103); R (91, 118); R (125, 152); A (128, 155); I (136, 163); E (143, 170); I-M (144–149, 171–176). (c) The predicted three-dimensional structure of proteins encoded by A1S_0248 using Swiss-MODEL. Red and green colors indicate the amino acids for the RNAP binding and DksA activity, respectively
    Wild Type A Baumannii Strain 381 Atcc 17978 Wt, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type a baumannii strain 381 atcc 17978 wt/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wild type a baumannii strain 381 atcc 17978 wt - by Bioz Stars, 2024-12
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    Bacterial strains and plasmids used in this work.

    Journal: Virulence

    Article Title: Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacter baumannii strain ATCC 17978

    doi: 10.1080/21505594.2020.1748923

    Figure Lengend Snippet: Bacterial strains and plasmids used in this work.

    Article Snippet: Briefly, an internal fragment of the target gene from wild-type (WT) A. baumannii strain ATCC 17978 was PCR-amplified using the appropriated primers (Supplementary Table 1).

    Techniques: Plasmid Preparation, Clone Assay, Over Expression, FLAG-tag

    Identification of DksA in A. baumannii . (a) Multiple alignments of translated sequences of A1S_0248 with DksA of other bacterial species. The gray highlight indicates identical amino acids with E. coli DksA. The bold characters indicate >50% consensus amino acids among the tested bacterial species. Sequence conservation is presented as the height of colored bars. The accession number of DksA proteins is ABO10723 in A. baumannii ATCC 17978, VWQ00927 in E. coli , CDO15944 in Klebsiella pneumoniae , WP_058139749 in Pseudomonas aeruginosa , KIS47657 in Burkholderia cepacia , RTY79203 in Staphylococcus aureus , WP_047919748 in Neisseria gonorrheae , WP_148842572 in Streptococcus pyogenes , SGC70216 in Mycobacterium tuberculosis , and VTQ30573 in Streptococcus pneumoniae . (b) Sequence logo of DksA between E. coli and A. baumannii ATCC 17978 using the WebLogo 3. The red boxes are essential amino acids for RNAP binding and DksA activity in E. coli . The number is based on the amino acid sequences of E. coli DksA. Furthermore, 151 amino acids of E. coli DksA (the first number in parenthesis) are compared with the translated amino acid sequences of A1S_0248 of A. baumannii ATCC 17978 (the second number in parenthesis) in the boxes as follows: D (74, 101); A (76, 103); R (91, 118); R (125, 152); A (128, 155); I (136, 163); E (143, 170); I-M (144–149, 171–176). (c) The predicted three-dimensional structure of proteins encoded by A1S_0248 using Swiss-MODEL. Red and green colors indicate the amino acids for the RNAP binding and DksA activity, respectively

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Identification of DksA in A. baumannii . (a) Multiple alignments of translated sequences of A1S_0248 with DksA of other bacterial species. The gray highlight indicates identical amino acids with E. coli DksA. The bold characters indicate >50% consensus amino acids among the tested bacterial species. Sequence conservation is presented as the height of colored bars. The accession number of DksA proteins is ABO10723 in A. baumannii ATCC 17978, VWQ00927 in E. coli , CDO15944 in Klebsiella pneumoniae , WP_058139749 in Pseudomonas aeruginosa , KIS47657 in Burkholderia cepacia , RTY79203 in Staphylococcus aureus , WP_047919748 in Neisseria gonorrheae , WP_148842572 in Streptococcus pyogenes , SGC70216 in Mycobacterium tuberculosis , and VTQ30573 in Streptococcus pneumoniae . (b) Sequence logo of DksA between E. coli and A. baumannii ATCC 17978 using the WebLogo 3. The red boxes are essential amino acids for RNAP binding and DksA activity in E. coli . The number is based on the amino acid sequences of E. coli DksA. Furthermore, 151 amino acids of E. coli DksA (the first number in parenthesis) are compared with the translated amino acid sequences of A1S_0248 of A. baumannii ATCC 17978 (the second number in parenthesis) in the boxes as follows: D (74, 101); A (76, 103); R (91, 118); R (125, 152); A (128, 155); I (136, 163); E (143, 170); I-M (144–149, 171–176). (c) The predicted three-dimensional structure of proteins encoded by A1S_0248 using Swiss-MODEL. Red and green colors indicate the amino acids for the RNAP binding and DksA activity, respectively

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Sequencing, Binding Assay, Activity Assay

    Growth of A. baumannii strains in minimal media and the expression of dksA in A. baumannii ATCC 17978 under different culture conditions. (a) WT A. baumannii ATCC 17978 (1), ∆ dksA mutant (2), and dksA -complemented (3) strains were grown on LB and M9 agar plates for 24 h. (b and c) The expression of dksA in A. baumannii ATCC 17978 was analyzed using qPCR. (b) Bacteria were cultured in LB under shaking or static conditions for 18 h. (c) Bacteria were cultured in LB under shaking at 23, 30, or 37°C for 18 h. The data are presented as the mean ± SD of three independent experiments. *** p < 0.001

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Growth of A. baumannii strains in minimal media and the expression of dksA in A. baumannii ATCC 17978 under different culture conditions. (a) WT A. baumannii ATCC 17978 (1), ∆ dksA mutant (2), and dksA -complemented (3) strains were grown on LB and M9 agar plates for 24 h. (b and c) The expression of dksA in A. baumannii ATCC 17978 was analyzed using qPCR. (b) Bacteria were cultured in LB under shaking or static conditions for 18 h. (c) Bacteria were cultured in LB under shaking at 23, 30, or 37°C for 18 h. The data are presented as the mean ± SD of three independent experiments. *** p < 0.001

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Expressing, Mutagenesis, Cell Culture

    Surface motility of A. baumannii strains. WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains were cultured in motility agar plates for 4, 7, and 12 h, and the diameters (mm) of bacterial migration on agar plates were measured. Data are presented as the mean ± SD of three independent experiments. *** p < 0.001 compared with the WT strain

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Surface motility of A. baumannii strains. WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains were cultured in motility agar plates for 4, 7, and 12 h, and the diameters (mm) of bacterial migration on agar plates were measured. Data are presented as the mean ± SD of three independent experiments. *** p < 0.001 compared with the WT strain

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Mutagenesis, Cell Culture, Migration

    Biofilm formation of A. baumannii strains. WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains were cultured in 24-well plates, and biofilm mass was stained with crystal violet. Total bacterial growth and biofilms were measured at OD 600 and OD 570 , respectively, and the biofilm mass was analyzed by OD 570 /OD 600 . The data are presented as the mean ± SD of three independent experiments. The pictures were biofilms stained with crystal violet. *** p < 0.001 compared with the WT strain

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Biofilm formation of A. baumannii strains. WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains were cultured in 24-well plates, and biofilm mass was stained with crystal violet. Total bacterial growth and biofilms were measured at OD 600 and OD 570 , respectively, and the biofilm mass was analyzed by OD 570 /OD 600 . The data are presented as the mean ± SD of three independent experiments. The pictures were biofilms stained with crystal violet. *** p < 0.001 compared with the WT strain

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Mutagenesis, Cell Culture, Staining

    Expression of genes associated with biofilm formation in A. baumannii strains. WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains were cultured in LB under static conditions for 24 h. The expression of genes was analyzed using qPCR. The data are the expression levels of mean ± SD of target genes in each strain relative to the expression of these genes in the WT strain. The experiments were performed three times independently. * p < 0.05, *** p < 0.001 compared with the WT strain

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Expression of genes associated with biofilm formation in A. baumannii strains. WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains were cultured in LB under static conditions for 24 h. The expression of genes was analyzed using qPCR. The data are the expression levels of mean ± SD of target genes in each strain relative to the expression of these genes in the WT strain. The experiments were performed three times independently. * p < 0.05, *** p < 0.001 compared with the WT strain

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Expressing, Mutagenesis, Cell Culture

    Production of autoinducers and expression of abaI and abaR in A. baumannii strains. (a) WT A. baumannii ATCC 17978 (1), ∆ dksA mutant (KM0248D, 2), and dksA -complemented (KM0248C, 3) strains were cultured in the bioassay agar plates overlaid with A. tumefaciens (pDCI41E33) for 18 h, and blue zone was measured. The experiments were performed three times independently. * p < 0.05 compared with the WT strain. (b) A. baumannii strains were cultured in LB under static conditions for 18 h. qPCR was performed to analyze the expression of genes. The data are presented as the mean ± SD of three independent experiments. *** p < 0.001 compared with the WT strain

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Production of autoinducers and expression of abaI and abaR in A. baumannii strains. (a) WT A. baumannii ATCC 17978 (1), ∆ dksA mutant (KM0248D, 2), and dksA -complemented (KM0248C, 3) strains were cultured in the bioassay agar plates overlaid with A. tumefaciens (pDCI41E33) for 18 h, and blue zone was measured. The experiments were performed three times independently. * p < 0.05 compared with the WT strain. (b) A. baumannii strains were cultured in LB under static conditions for 18 h. qPCR was performed to analyze the expression of genes. The data are presented as the mean ± SD of three independent experiments. *** p < 0.001 compared with the WT strain

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Expressing, Mutagenesis, Cell Culture

    Survival rates and pathologies in mice infected with WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains. (a) Neutropenic mice were infected with 5 × 10 8 CFUs of A. baumannii strains intraperitoneally. The survival of mice was determined for five days post-infection. (b and c) Seven neutropenic mice in each group were infected with 5 × 10 7 CFUs of A. baumannii strains intratracheally. The surviving mice were sacrificed 24 h post-infection. (b) CFUs in the right lung and blood were determined. ** p < 0.01 compared with the WT strain. (c) Left lung tissue was stained with hematoxylin and eosin. Magnification, 100X

    Journal: Virulence

    Article Title: Global regulator DksA modulates virulence of Acinetobacter baumannii

    doi: 10.1080/21505594.2021.1995253

    Figure Lengend Snippet: Survival rates and pathologies in mice infected with WT A. baumannii ATCC 17978, ∆ dksA mutant (KM0248D), and dksA -complemented (KM0248C) strains. (a) Neutropenic mice were infected with 5 × 10 8 CFUs of A. baumannii strains intraperitoneally. The survival of mice was determined for five days post-infection. (b and c) Seven neutropenic mice in each group were infected with 5 × 10 7 CFUs of A. baumannii strains intratracheally. The surviving mice were sacrificed 24 h post-infection. (b) CFUs in the right lung and blood were determined. ** p < 0.01 compared with the WT strain. (c) Left lung tissue was stained with hematoxylin and eosin. Magnification, 100X

    Article Snippet: Furthermore, (p)ppGpp-deficient mutants are more susceptible to antimicrobial agents, including gentamicin, tetracycline, erythromycin, and trimethoprim, than wild-type (WT) A. baumannii ATCC 17978 strain by downregulating efflux pump genes [ ].

    Techniques: Infection, Mutagenesis, Staining