wild type sfβgly novablue de3 competent cells  (Millipore)


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    Millipore wild type sfβgly novablue de3 competent cells
    Wild Type Sfβgly Novablue De3 Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type sfβgly novablue de3 competent cells/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wild type sfβgly novablue de3 competent cells - by Bioz Stars, 2020-04
    87/100 stars

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    Centrifugation:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

    Mutagenesis:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: .. Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

    Purification:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: .. Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

    Concentration Assay:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

    Incubation:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. The supernatants were recovered by centrifugation (13,200 g, 20 min, 4°C), and soluble recombinant proteins were incubated with 200 µL Ni-NTA Agarose (4°C, 1 h) (Qiagen, Hilden, Germany).

    Expressing:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: .. Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

    Transformation Assay:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: .. Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

    Recombinant:

    Article Title: Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 ?-Glucosidases
    Article Snippet: .. Expression and purification of recombinant and wild-type Sfβgly NovaBlue (DE3) competent cells (EMD Millipore, Billerica, MA, USA) were transformed with pAE plasmids encoding wild-type or mutant Sfβgly, plated on LB-agar containing ampicillin (50 µg/mL) and grown at 37°C for 16 h. Single colonies were grown at 20°C in LB broth containing ampicillin (50 µg/mL) until they reached an attenuance of 0.500 at 600 nm. .. Next, 0.4 mM (final concentration) isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for 16 h to induce recombinant protein expression, after which cells were harvested by centrifugation (4,000×g , 20 min, 4°C) and frozen at −80°C.

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  • 87
    Millipore wild type sfβgly novablue de3 competent cells
    Wild Type Sfβgly Novablue De3 Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type sfβgly novablue de3 competent cells/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wild type sfβgly novablue de3 competent cells - by Bioz Stars, 2020-04
    87/100 stars
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