wheeldon lj  (Worthington Biochemical)


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  • 86
    Name:
    Histone Dried
    Description:
    An ethanol dried powder Unfractionated mixture of histones
    Catalog Number:
    ls002375
    Price:
    34
    Source:
    Calf Thymus
    Size:
    250 mg
    Cas Number:
    37244.51.2
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    Structured Review

    Worthington Biochemical wheeldon lj
    An ethanol dried powder Unfractionated mixture of histones
    https://www.bioz.com/result/wheeldon lj/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheeldon lj - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    In Vivo:

    Article Title: Extracellular histones trigger disseminated intravascular coagulation by lytic cell death
    Article Snippet: MiceC57BL/6J, Casp1/11-/-, Casp11-/-, Tlr4-/-, Tlr2-/-, Gsdmd-/-, and Casp3-/- mice were housed in the University of Kentucky Animal Care Facility, following institutional and National Institutes of Health guidelines after approval by the Institutional Animal Care and Use Committee. .. In vivo ChallengesHistones (Worthington cat#LS002546/lot#33P14617) were administered at 50 mg/kg via retro-obital injection. .. Pharmacological TF inhibitionRat IgG (Sigma, Cat#I4131) or 1H1 anti-TF antibody (Genentech) at 8 mg/kg was given via retro-orbital injection 2 hours prior to histone challenge.

    Injection:

    Article Title: Extracellular histones trigger disseminated intravascular coagulation by lytic cell death
    Article Snippet: MiceC57BL/6J, Casp1/11-/-, Casp11-/-, Tlr4-/-, Tlr2-/-, Gsdmd-/-, and Casp3-/- mice were housed in the University of Kentucky Animal Care Facility, following institutional and National Institutes of Health guidelines after approval by the Institutional Animal Care and Use Committee. .. In vivo ChallengesHistones (Worthington cat#LS002546/lot#33P14617) were administered at 50 mg/kg via retro-obital injection. .. Pharmacological TF inhibitionRat IgG (Sigma, Cat#I4131) or 1H1 anti-TF antibody (Genentech) at 8 mg/kg was given via retro-orbital injection 2 hours prior to histone challenge.

    Incubation:

    Article Title: Basic surface features of nuclear FKBPs facilitate chromatin binding
    Article Snippet: Protein interactions Protein interaction experiments were performed using recombinant 6His tagged Fpr4 proteins. .. Briefly, one microgram of 6His tagged Fpr4 proteins was incubated with two micrograms of recombinant H3 or calf thymus bulk histones (Worthington, cat# LS002548) in interaction buffer (20 mM Tris pH 8, 0.2 mM EDTA, 300 mM KCl, 30 mM Imidazole, 0.1% NP40, 20% glycerol, 2 mM PMSF) for 30 minutes at 4 °C. ..

    Article Title: Recognition of Unmodified Histone H3 by the First PHD Finger of Bromodomain-PHD Finger Protein 2 Provides Insights into the Regulation of Histone Acetyltransferases Monocytic Leukemic Zinc-finger Protein (MOZ) and MOZ-related factor (MORF) *
    Article Snippet: .. The beads were then incubated for 1 h at 4 °C with histones (50 μg, Worthington) in buffer containing 20 m m Tris, pH 7.5, 500 m m NaCl, and 0.2% Triton X-100. .. After six washes, the bound material was resolved on SDS-polyacrylamide gels and stained with Coomassie Brilliant Blue.

    Recombinant:

    Article Title: Basic surface features of nuclear FKBPs facilitate chromatin binding
    Article Snippet: Protein interactions Protein interaction experiments were performed using recombinant 6His tagged Fpr4 proteins. .. Briefly, one microgram of 6His tagged Fpr4 proteins was incubated with two micrograms of recombinant H3 or calf thymus bulk histones (Worthington, cat# LS002548) in interaction buffer (20 mM Tris pH 8, 0.2 mM EDTA, 300 mM KCl, 30 mM Imidazole, 0.1% NP40, 20% glycerol, 2 mM PMSF) for 30 minutes at 4 °C. ..

    In Vitro:

    Article Title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia
    Article Snippet: GST and GST–Ar-SETD4 were expressed in bacteria and purified by using GST-Sepharose beads for subsequent in vitro HMT assays. .. The in vitro HMT assays were modified versions of protocols described previously ( ) and were performed by using 50 μl of methylase activity buffer (50 mM Tris [pH 8.5], 20 mM KCl, 10 mM MgCl2 , 10 mM β-mercaptoethanol, and 250 mM sucrose) containing either 10 μg of core histones (mixture of H1, H3, H2B, H2A, and H4 from calf thymus) (catalogue number LS002544; Worthington) or 1 μg of synthesized H4 peptide with H4K20me0, H4K20me1, or H4K20me2 modifications (the sequence is described above) as the substrates, 10 mM Adomet (Sangon Biotech) was used as a methyl donor, and GST or GST–Ar-SETD4 was used as the enzyme. .. After incubation for 60 min at 30°C, the reactions were stopped by boiling in 2× SDS loading buffer.

    HMT Assay:

    Article Title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia
    Article Snippet: GST and GST–Ar-SETD4 were expressed in bacteria and purified by using GST-Sepharose beads for subsequent in vitro HMT assays. .. The in vitro HMT assays were modified versions of protocols described previously ( ) and were performed by using 50 μl of methylase activity buffer (50 mM Tris [pH 8.5], 20 mM KCl, 10 mM MgCl2 , 10 mM β-mercaptoethanol, and 250 mM sucrose) containing either 10 μg of core histones (mixture of H1, H3, H2B, H2A, and H4 from calf thymus) (catalogue number LS002544; Worthington) or 1 μg of synthesized H4 peptide with H4K20me0, H4K20me1, or H4K20me2 modifications (the sequence is described above) as the substrates, 10 mM Adomet (Sangon Biotech) was used as a methyl donor, and GST or GST–Ar-SETD4 was used as the enzyme. .. After incubation for 60 min at 30°C, the reactions were stopped by boiling in 2× SDS loading buffer.

    Modification:

    Article Title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia
    Article Snippet: GST and GST–Ar-SETD4 were expressed in bacteria and purified by using GST-Sepharose beads for subsequent in vitro HMT assays. .. The in vitro HMT assays were modified versions of protocols described previously ( ) and were performed by using 50 μl of methylase activity buffer (50 mM Tris [pH 8.5], 20 mM KCl, 10 mM MgCl2 , 10 mM β-mercaptoethanol, and 250 mM sucrose) containing either 10 μg of core histones (mixture of H1, H3, H2B, H2A, and H4 from calf thymus) (catalogue number LS002544; Worthington) or 1 μg of synthesized H4 peptide with H4K20me0, H4K20me1, or H4K20me2 modifications (the sequence is described above) as the substrates, 10 mM Adomet (Sangon Biotech) was used as a methyl donor, and GST or GST–Ar-SETD4 was used as the enzyme. .. After incubation for 60 min at 30°C, the reactions were stopped by boiling in 2× SDS loading buffer.

    Activity Assay:

    Article Title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia
    Article Snippet: GST and GST–Ar-SETD4 were expressed in bacteria and purified by using GST-Sepharose beads for subsequent in vitro HMT assays. .. The in vitro HMT assays were modified versions of protocols described previously ( ) and were performed by using 50 μl of methylase activity buffer (50 mM Tris [pH 8.5], 20 mM KCl, 10 mM MgCl2 , 10 mM β-mercaptoethanol, and 250 mM sucrose) containing either 10 μg of core histones (mixture of H1, H3, H2B, H2A, and H4 from calf thymus) (catalogue number LS002544; Worthington) or 1 μg of synthesized H4 peptide with H4K20me0, H4K20me1, or H4K20me2 modifications (the sequence is described above) as the substrates, 10 mM Adomet (Sangon Biotech) was used as a methyl donor, and GST or GST–Ar-SETD4 was used as the enzyme. .. After incubation for 60 min at 30°C, the reactions were stopped by boiling in 2× SDS loading buffer.

    Synthesized:

    Article Title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia
    Article Snippet: GST and GST–Ar-SETD4 were expressed in bacteria and purified by using GST-Sepharose beads for subsequent in vitro HMT assays. .. The in vitro HMT assays were modified versions of protocols described previously ( ) and were performed by using 50 μl of methylase activity buffer (50 mM Tris [pH 8.5], 20 mM KCl, 10 mM MgCl2 , 10 mM β-mercaptoethanol, and 250 mM sucrose) containing either 10 μg of core histones (mixture of H1, H3, H2B, H2A, and H4 from calf thymus) (catalogue number LS002544; Worthington) or 1 μg of synthesized H4 peptide with H4K20me0, H4K20me1, or H4K20me2 modifications (the sequence is described above) as the substrates, 10 mM Adomet (Sangon Biotech) was used as a methyl donor, and GST or GST–Ar-SETD4 was used as the enzyme. .. After incubation for 60 min at 30°C, the reactions were stopped by boiling in 2× SDS loading buffer.

    Sequencing:

    Article Title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia
    Article Snippet: GST and GST–Ar-SETD4 were expressed in bacteria and purified by using GST-Sepharose beads for subsequent in vitro HMT assays. .. The in vitro HMT assays were modified versions of protocols described previously ( ) and were performed by using 50 μl of methylase activity buffer (50 mM Tris [pH 8.5], 20 mM KCl, 10 mM MgCl2 , 10 mM β-mercaptoethanol, and 250 mM sucrose) containing either 10 μg of core histones (mixture of H1, H3, H2B, H2A, and H4 from calf thymus) (catalogue number LS002544; Worthington) or 1 μg of synthesized H4 peptide with H4K20me0, H4K20me1, or H4K20me2 modifications (the sequence is described above) as the substrates, 10 mM Adomet (Sangon Biotech) was used as a methyl donor, and GST or GST–Ar-SETD4 was used as the enzyme. .. After incubation for 60 min at 30°C, the reactions were stopped by boiling in 2× SDS loading buffer.

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  • 93
    Worthington Biochemical wheeldon lj
    Wheeldon Lj, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheeldon lj/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheeldon lj - by Bioz Stars, 2021-03
    93/100 stars
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