Structured Review

Waters Corporation waters seppak cartridges
Waters Seppak Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/waters seppak cartridges/product/Waters Corporation
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
waters seppak cartridges - by Bioz Stars, 2020-04
92/100 stars

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solid-phase extraction
c18 solid-phase extraction

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In Vivo:

Article Title: A Highly Conserved Basidiomycete Peptide Synthetase Produces a Trimeric Hydroxamate Siderophore
Article Snippet: The gradient was 10% to 20% B within 15 min, increased to 50% B in 15 min, from 50% to 60% B in 10 min, and to 100% B in another 10 min. For in vivo analyses, Ceriporiopsis subvermispora culture supernatants were filtered and supplemented with Amberlite XAD-16 adsorber resin (10%, wt/vol). .. The extract (2 ml) was further fractionated by solid-phase extraction using Waters SepPak cartridges (20 ml, C18 , 5 g).

Mass Spectrometry:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: Paragraph title: Mass spectrometry ... The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960).

Evaporation:

Article Title: A Highly Conserved Basidiomycete Peptide Synthetase Produces a Trimeric Hydroxamate Siderophore
Article Snippet: Subsequently, the solvent and residual water were removed by rotary evaporation under reduced pressure. .. The extract (2 ml) was further fractionated by solid-phase extraction using Waters SepPak cartridges (20 ml, C18 , 5 g).

Incubation:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: Proteins were precipitated by adding one-fourth volume of 100% (w/w) trichloroacetic acid in water, re-suspended in 1% SDS, 0.2 M NaOH and 10 mM DTT and incubated for 60 min at 37°C. .. The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960).

Diffusion-based Assay:

Article Title: A Highly Conserved Basidiomycete Peptide Synthetase Produces a Trimeric Hydroxamate Siderophore
Article Snippet: The extract (2 ml) was further fractionated by solid-phase extraction using Waters SepPak cartridges (20 ml, C18 , 5 g). .. Fractions were analyzed in the CAS diffusion assay, and CAS assay-positive fractions were analyzed by LC-MS. High-resolution mass spectra were acquired on a Thermo Accela liquid chromatograph with a C18 column (Grom-Sil 100 ODS-0 AB; 250 by 4.6 mm, 3 μm), interfaced to an Exactive Orbitrap spectrometer operated in positive and negative modes and by electrospray ionization.

Liquid Chromatography with Mass Spectroscopy:

Article Title: A Highly Conserved Basidiomycete Peptide Synthetase Produces a Trimeric Hydroxamate Siderophore
Article Snippet: The extract (2 ml) was further fractionated by solid-phase extraction using Waters SepPak cartridges (20 ml, C18 , 5 g). .. Fractions were analyzed in the CAS diffusion assay, and CAS assay-positive fractions were analyzed by LC-MS. High-resolution mass spectra were acquired on a Thermo Accela liquid chromatograph with a C18 column (Grom-Sil 100 ODS-0 AB; 250 by 4.6 mm, 3 μm), interfaced to an Exactive Orbitrap spectrometer operated in positive and negative modes and by electrospray ionization.

Tandem Mass Spectroscopy:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960). .. Acquired MS/MS spectra were searched against a concatentated target/decoy protein sequence database consisting of the predicted proteins from C. phytofermentans (NCBI NC_010001.faa), common contaminants such as trypsin and human keratins, and the reversed sequences of these proteins used as decoy co mponent ( ).

Sequencing:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960). .. Acquired MS/MS spectra were searched against a concatentated target/decoy protein sequence database consisting of the predicted proteins from C. phytofermentans (NCBI NC_010001.faa), common contaminants such as trypsin and human keratins, and the reversed sequences of these proteins used as decoy co mponent ( ).

Purification:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960). .. Purified peptides were analysed by microcapillary liquid chromatography tandem mass spectrometry using an in-house prepared 125-μm-ID C18 reversed-phase column and a hybrid linear quadrupole/FT-ICR mass spectrometer (LTQ FT, Thermo Scientific, Bremen, Germany) as described in ).

Liquid Chromatography:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960). .. Purified peptides were analysed by microcapillary liquid chromatography tandem mass spectrometry using an in-house prepared 125-μm-ID C18 reversed-phase column and a hybrid linear quadrupole/FT-ICR mass spectrometer (LTQ FT, Thermo Scientific, Bremen, Germany) as described in ).

SDS Page:

Article Title: Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367
Article Snippet: Cysteine residues were then derivatized with iodoacetic acid (30 mM) at room temperature in the dark for 60 min. Five hundred micrograms of protein was resolved on 4–12% Bis-Tris SDS-PAGE gels (Invitrogen NP0335BOX). .. The resulting peptides were subjected to C18 solid-phase extraction using Waters SepPak cartridges (Waters WAT054960).

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    Waters Corporation seppak cartridge
    Seppak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/seppak cartridge/product/Waters Corporation
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    seppak cartridge - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    Waters Corporation cartridge
    Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cartridge/product/Waters Corporation
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cartridge - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    98
    Waters Corporation solid phase extraction
    Solid Phase Extraction, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 98/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction/product/Waters Corporation
    Average 98 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction - by Bioz Stars, 2020-04
    98/100 stars
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    87
    Waters Corporation tc18 light reverse phase silica cartridge
    Tc18 Light Reverse Phase Silica Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tc18 light reverse phase silica cartridge/product/Waters Corporation
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tc18 light reverse phase silica cartridge - by Bioz Stars, 2020-04
    87/100 stars
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