Journal: Dose-Response

Article Title: Corilagin Alleviates Ang II-Induced Cardiac Fibrosis by Regulating the PTEN/AKT/mTOR Pathway

doi: 10.1177/15593258241261198

Figure Lengend Snippet: Cor modulates the PTEN-AKT-mTOR pathway for its inhibitory effects on Ang II-induced cardiac fibroblast migration. (A) Representative images of Western blot of p-PTEN (Ser380), PTEN, p-Akt (Ser473) and p-mTOR (Ser 2448) are shown. (B) Quantitative analysis of protein bands that are normalized to GAPDH (p-PTEN and PTEN), Akt (p-Akt) or mTOR (p-mTOR). The cardiac fibroblasts were stimulated with Ang II (1 μM) infusion, incubated for 48 hours, and then treated with Cor (50 μM) for 2 hours. Then, the PTEN inhibitor VO- ohpic (1 μM) was treated for 2 hours. (C) The mRNA expression of PTEN, AKT and mTOR was determined by RT-qPCR. GADPH served as an internal control. (D) The cell migrative capability of cardiac fibroblasts was determined by Transwell assay. (E) Quantification of the average number of migrated fibroblasts in each field. Data are shown as mean ± SD (n = 6 per group). Cor group cells were cotreated with Ang II and Cor; Cor+VO-ohpic group cells were cotreated with Ang II, Cor, and VO-ohpic. *** P < .001 vs control group; ### P < .001 vs Ang II group; $$$ P < .001 vs Cor group.

Article Snippet: For the inhibitor experiment, VO-ohpic (1 μM) (HY-110067, MedChemExpress) was used to inhibit PTEN activity.

Techniques: Migration, Western Blot, Incubation, Expressing, Quantitative RT-PCR, Control, Transwell Assay

Journal: iScience

Article Title: Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells

doi: 10.1016/j.isci.2024.110306

Figure Lengend Snippet:

Article Snippet: VO-Ohpic trihydrate , MedChemExpress , HY-13074.

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, Software

Journal: EMBO Molecular Medicine

Article Title: Targeting PI3K/Akt/mTOR signaling in rodent models of PMP22 gene-dosage diseases

doi: 10.1038/s44321-023-00019-5

Figure Lengend Snippet: ( A ) PTEN inhibitor VO-OHpic was used to disinhibit the PI3K/Akt/mTOR signaling pathway in CMT1A. ( B ) Example images of Schwann cell-dorsal root ganglia neuron co-cultures from wildtype (WT) and Pmp22 tg rats treated with DMSO as control (Ctrl) or PTEN inhibitor VO-OHpic (500 nM). Cells were stained for myelin basic protein (MBP) as a marker for myelinated segments (gray/ green) and TUJ1 for neurons (magenta) as well as DAPI for cell nuclei (blue). ( C ) Quantification of ( B ) shows a dose-dependent decrease of myelinated segments in WT co-cultures treated with DMSO and different concentrations of VO-OHpic (50 nM, 500 nM, 5 µM) and an increase of myelinated segments in Pmp22 tg co-cultures with 500 nM VO-OHpic (WT n = 5, CMT1A n = 7 animals). Groups were compared using two-way ANOVA with Sidak’s multiple comparison test (* p ≤ 0.05, **** p ≤ 0.0001). .

Article Snippet: For PTEN inhibition in Pmp22 tg cultures, cells were treated with 1% DMSO (Sigma) as a control or with the PTEN inhibitor VO-OHpic (Rosivatz et al, ) (Sigma) at 50 nM, 500 nM and 5 μM, respectively.

Techniques: Staining, Marker, Comparison

Journal: EMBO Molecular Medicine

Article Title: Targeting PI3K/Akt/mTOR signaling in rodent models of PMP22 gene-dosage diseases

doi: 10.1038/s44321-023-00019-5

Figure Lengend Snippet: Example images of SC-DRG co-cultures from wildtype (WT) and Pmp22 tg rats, treated with different dosages of the PTEN inhibitor VO-OHpic for 14 days. The number of myelinated segments (MBP; gray/green) decreases in WT cultures with increasing inhibitor dosage. In Pmp22 tg co-cultures an increase is observed up to 500 nM VO-OHpic but a decrease with 5 µM VO-OHpic. Scale bar is 50 µm. Images for 500 nM VO-OHpic treatment are the same as used in Fig. .

Article Snippet: For PTEN inhibition in Pmp22 tg cultures, cells were treated with 1% DMSO (Sigma) as a control or with the PTEN inhibitor VO-OHpic (Rosivatz et al, ) (Sigma) at 50 nM, 500 nM and 5 μM, respectively.

Techniques:

Journal: eLife

Article Title: The myocardium utilizes a platelet-derived growth factor receptor alpha (Pdgfra)–phosphoinositide 3-kinase (PI3K) signaling cascade to steer toward the midline during zebrafish heart tube formation

doi: 10.7554/eLife.85930

Figure Lengend Snippet:

Article Snippet: The following inhibitors were used: LY294002 (LY, Millipore-Sigma 154447-36-6), Dactolisib (Dac, Millipore-Sigma 915019-65-7), Pictilisib (Pic, Millipore-Sigma 957054-30-7), Rapamycin (Rap, Selleckchem S1039), and VO-Ohpic trihydrate (VOOH, Selleckchem S8174).

Techniques: Transgenic Assay, Mutagenesis, Recombinant, Proliferation Assay, Software, Microscopy