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Sartorius AG vivaspin6 protein concentrators
Vivaspin6 Protein Concentrators, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vivaspin6 protein concentrators/product/Sartorius AG
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
vivaspin6 protein concentrators - by Bioz Stars, 2020-04
86/100 stars

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Concentration Assay:

Article Title: Biophysical Characterisation of Calumenin as a Charged F508del-CFTR Folding Modulator
Article Snippet: Proteins were concentrated using Vivaspin6 protein concentrators, 3 kDa (Sartorius Stedim Biotech). .. F508del-CFTR protein aggregation assay to monitor formation of off-pathway species were initiated by rapid dilution of F508del-CFTR in 6 M GuHCl to a final concentration of 1 µM into pre-warmed (37°C) folding mix (Buffer R: 100 mM Tris-HCl, pH 7.4, 0.385 M L-arginine-HCl, 2 mM EDTA, 10 mM dithiothreitol and 25 mM GuHCl).

Incubation:

Article Title: Biophysical Characterisation of Calumenin as a Charged F508del-CFTR Folding Modulator
Article Snippet: Proteins were concentrated using Vivaspin6 protein concentrators, 3 kDa (Sartorius Stedim Biotech). .. Protein concentrations were determined by plotting a standard curve using different concentrations of bovine serum albumin and Folin's reagent, by measuring absorbance at 660 nm of reaction mixtures after incubation at 37°C for 20 minutes in the dark.

Recombinant:

Article Title: Biophysical Characterisation of Calumenin as a Charged F508del-CFTR Folding Modulator
Article Snippet: F508del-CFTR anti-aggregation assay Pure recombinant human calumenin was obtained from Prospec (Israel) and purified full length F508del-CFTR was a generous gift from Prof. Robert Ford (University of Manchester, UK). .. Proteins were concentrated using Vivaspin6 protein concentrators, 3 kDa (Sartorius Stedim Biotech).

Purification:

Article Title: Biophysical Characterisation of Calumenin as a Charged F508del-CFTR Folding Modulator
Article Snippet: AavLEA1 was purified using Ni-NTA Fast Start kit (Qiagen) from BL21 (DE3) E.coli cells containing pET15b-AavLEA1 according to the manufacturer's instructions and dialyzed extensively into water. .. Proteins were concentrated using Vivaspin6 protein concentrators, 3 kDa (Sartorius Stedim Biotech).

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    Sartorius AG vivaspin6 protein concentrators
    Vivaspin6 Protein Concentrators, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin6 protein concentrators/product/Sartorius AG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vivaspin6 protein concentrators - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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