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GE Healthcare vivaspin protein concentrators
Vivaspin Protein Concentrators, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vivaspin protein concentrators/product/GE Healthcare
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
vivaspin protein concentrators - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Clone Assay:

Article Title: Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Article Snippet: The restriction endonucleases, pfu turbo polymerase and T4 DNA ligase used in cloning of the genes ycjM , ycjT , and ycjU from E. coli were purchased from New England BioLabs. .. The 5-mL HisTrap columns, PD-10 desalting columns, and Vivaspin protein concentrators (molecular weight cut-off of 30 kDa) were bought from GE Healthcare.

Centrifugation:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: .. Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare). .. Comassie gels of purified proteins are shown in .

shRNA:

Article Title: Store-Operated Calcium Entry via STIM1 Contributes to MRGPRX2 Induced Mast Cell Functions
Article Snippet: Cells were serum-starved for 1 h in Opti-MEM™ medium before addition of Lipofectamine® 2000 reagent and the viral packaging plasmids: p-CMV-VSV-G, pHR'8.2ΔR, and STIM1 shRNA (Cat# SHCLNG-NM_003156; TRC# TRCN0000358718) or scrambled-sequence control shRNA (Cat# SCH002). .. Media was changed to DMEM after 6 h. Seventy-two hours post-transfection, viral supernatant was harvested, 0.4 μm sterile-filtered, and concentrated using Vivaspin™ protein concentrators (100 kDa MWCO, GE Healthcare).

Synthesized:

Article Title: Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Article Snippet: The 5-mL HisTrap columns, PD-10 desalting columns, and Vivaspin protein concentrators (molecular weight cut-off of 30 kDa) were bought from GE Healthcare. .. The carbohydrates used as potential substrates for YcjM, YcjT, and YcjU were obtained from Carbosynth, except for α-D-glucose-1,6-bisphosphate, D-glycerate, and α-D-glucose-1-P, which were obtained from Sigma Aldrich. α-(1,2)-D-Glucose-D-glycerate was synthesized by a modification of a previously published procedure. The following bacterial strains were purchased from the Keio collection: parent strain for the knockouts BW25113 (CGSC 7636), Δ ycjM (CGSC 9167), Δ ycjR (CGSC 11236) and Δ ycjT ).

Bradford Assay:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare). .. Protein concentration was approximated using a Bradford assay and approximately 1 μg of each protein used in kinase assays.

Transfection:

Article Title: Store-Operated Calcium Entry via STIM1 Contributes to MRGPRX2 Induced Mast Cell Functions
Article Snippet: Generation of Lentivirus Confluent HEK-293T cells (1 × 106 cells) were seeded on to 100 mm2 dishes 48 h prior to transfection. .. Media was changed to DMEM after 6 h. Seventy-two hours post-transfection, viral supernatant was harvested, 0.4 μm sterile-filtered, and concentrated using Vivaspin™ protein concentrators (100 kDa MWCO, GE Healthcare).

Construct:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Protein expression constructs were transformed into E. coli Rosetta cells (Invitrogen). .. Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare).

Purification:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare). .. Comassie gels of purified proteins are shown in .

Protein Purification:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Paragraph title: Protein purification ... Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare).

Polymerase Chain Reaction:

Article Title: Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Article Snippet: The PCR cleanup and gel extraction kits were obtained from Qiagen. .. The 5-mL HisTrap columns, PD-10 desalting columns, and Vivaspin protein concentrators (molecular weight cut-off of 30 kDa) were bought from GE Healthcare.

Gel Extraction:

Article Title: Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Article Snippet: The PCR cleanup and gel extraction kits were obtained from Qiagen. .. The 5-mL HisTrap columns, PD-10 desalting columns, and Vivaspin protein concentrators (molecular weight cut-off of 30 kDa) were bought from GE Healthcare.

Expressing:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Protein expression constructs were transformed into E. coli Rosetta cells (Invitrogen). .. Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare).

Protein Concentration:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare). .. Protein concentration was approximated using a Bradford assay and approximately 1 μg of each protein used in kinase assays.

Modification:

Article Title: Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Article Snippet: The 5-mL HisTrap columns, PD-10 desalting columns, and Vivaspin protein concentrators (molecular weight cut-off of 30 kDa) were bought from GE Healthcare. .. The carbohydrates used as potential substrates for YcjM, YcjT, and YcjU were obtained from Carbosynth, except for α-D-glucose-1,6-bisphosphate, D-glycerate, and α-D-glucose-1-P, which were obtained from Sigma Aldrich. α-(1,2)-D-Glucose-D-glycerate was synthesized by a modification of a previously published procedure. The following bacterial strains were purchased from the Keio collection: parent strain for the knockouts BW25113 (CGSC 7636), Δ ycjM (CGSC 9167), Δ ycjR (CGSC 11236) and Δ ycjT ).

Sonication:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Pellets were resuspended in lysis buffer (for GST-tagged proteins: 50 mM Tris, pH 8.0; 50 mM NaCl, 0.15 mM PMSF; for His-tagged proteins: 50 mM Tris, pH 8.0; 20 mM imidazole; 500 mM NaCl, 10% glycerol; 1% Tween) and lysed by sonication. .. Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare).

Lysis:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Pellets were resuspended in lysis buffer (for GST-tagged proteins: 50 mM Tris, pH 8.0; 50 mM NaCl, 0.15 mM PMSF; for His-tagged proteins: 50 mM Tris, pH 8.0; 20 mM imidazole; 500 mM NaCl, 10% glycerol; 1% Tween) and lysed by sonication. .. Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare).

Variant Assay:

Article Title: In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex
Article Snippet: Empty disposable PD-10 columns, Superdex 200 10/300 GL, and Vivaspin protein concentrators were purchased from GE Healthcare. .. The unlabeled truncated neurotensin ligand (NT8-13 , RRPYIL) and its variant NT1 (GPGGRRPYIL) were purchased from Anaspec.

Transformation Assay:

Article Title: An Arabidopsis kinase cascade influences auxin-responsive cell expansion
Article Snippet: Protein expression constructs were transformed into E. coli Rosetta cells (Invitrogen). .. Eluates were concentrated by centrifugation with Vivaspin protein concentrators (GE Healthcare).

Molecular Weight:

Article Title: Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Article Snippet: .. The 5-mL HisTrap columns, PD-10 desalting columns, and Vivaspin protein concentrators (molecular weight cut-off of 30 kDa) were bought from GE Healthcare. .. The M9 minimal media salts and thiamine were obtained from Sigma Aldrich.

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    GE Healthcare vivaspin protein concentrator
    Vivaspin Protein Concentrator, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin protein concentrator/product/GE Healthcare
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vivaspin protein concentrator - by Bioz Stars, 2020-04
    96/100 stars
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