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Sartorius AG vivaspin protein concentrator
Vivaspin Protein Concentrator, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vivaspin protein concentrator/product/Sartorius AG
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
vivaspin protein concentrator - by Bioz Stars, 2020-04
89/100 stars

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Labeling:

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: Paragraph title: Protein Labeling with Amine- and Cysteine-Reactive Probes. ... The reduced protein was combined with a 20-fold molar excess of AlexaFluor 594 C5 maleimide (Life Technologies), AlexaFluor 647 C2 maleimide (Life Technologies), or maleimido–monoamide–DOTA (Macrocyclics) and incubated at room temperature for 1 h and then 4 °C for an additional 12 h. Excess-free probe was removed by desalting the reaction mixture into PBS using a VivaSpin protein concentrator (Sartorius Stedim).

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: .. Free dye was quenched by addition of Tris pH 8.0 to a final concentration of 20 mM and the labeled antibody desalted with a VivaSpin protein concentrator. .. The IgV domains of hPD-1 (residues 26–147), the IgV and IgC domains of hPD-L1 (residues 19–132), and hPD-L2 (residues 20–123) were displayed on the surface of Saccharomyces cerevisiae strain EBY100 as N-terminal fusions to Aga2 using the pYAL vector as previously described ( ).

Purification:

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: HAC-V N91C was expressed and purified as described above and reduced by application of Tris(2-carboxyethyl)phosphine to a final concentration of 1 mM. .. The reduced protein was combined with a 20-fold molar excess of AlexaFluor 594 C5 maleimide (Life Technologies), AlexaFluor 647 C2 maleimide (Life Technologies), or maleimido–monoamide–DOTA (Macrocyclics) and incubated at room temperature for 1 h and then 4 °C for an additional 12 h. Excess-free probe was removed by desalting the reaction mixture into PBS using a VivaSpin protein concentrator (Sartorius Stedim).

HAC Assay:

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: HAC-V N91C was expressed and purified as described above and reduced by application of Tris(2-carboxyethyl)phosphine to a final concentration of 1 mM. .. The reduced protein was combined with a 20-fold molar excess of AlexaFluor 594 C5 maleimide (Life Technologies), AlexaFluor 647 C2 maleimide (Life Technologies), or maleimido–monoamide–DOTA (Macrocyclics) and incubated at room temperature for 1 h and then 4 °C for an additional 12 h. Excess-free probe was removed by desalting the reaction mixture into PBS using a VivaSpin protein concentrator (Sartorius Stedim).

Concentration Assay:

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: HAC-V N91C was expressed and purified as described above and reduced by application of Tris(2-carboxyethyl)phosphine to a final concentration of 1 mM. .. The reduced protein was combined with a 20-fold molar excess of AlexaFluor 594 C5 maleimide (Life Technologies), AlexaFluor 647 C2 maleimide (Life Technologies), or maleimido–monoamide–DOTA (Macrocyclics) and incubated at room temperature for 1 h and then 4 °C for an additional 12 h. Excess-free probe was removed by desalting the reaction mixture into PBS using a VivaSpin protein concentrator (Sartorius Stedim).

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: .. Free dye was quenched by addition of Tris pH 8.0 to a final concentration of 20 mM and the labeled antibody desalted with a VivaSpin protein concentrator. .. The IgV domains of hPD-1 (residues 26–147), the IgV and IgC domains of hPD-L1 (residues 19–132), and hPD-L2 (residues 20–123) were displayed on the surface of Saccharomyces cerevisiae strain EBY100 as N-terminal fusions to Aga2 using the pYAL vector as previously described ( ).

Incubation:

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: .. The reduced protein was combined with a 20-fold molar excess of AlexaFluor 594 C5 maleimide (Life Technologies), AlexaFluor 647 C2 maleimide (Life Technologies), or maleimido–monoamide–DOTA (Macrocyclics) and incubated at room temperature for 1 h and then 4 °C for an additional 12 h. Excess-free probe was removed by desalting the reaction mixture into PBS using a VivaSpin protein concentrator (Sartorius Stedim). ..

Mass Spectrometry:

Article Title: Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Article Snippet: The reduced protein was combined with a 20-fold molar excess of AlexaFluor 594 C5 maleimide (Life Technologies), AlexaFluor 647 C2 maleimide (Life Technologies), or maleimido–monoamide–DOTA (Macrocyclics) and incubated at room temperature for 1 h and then 4 °C for an additional 12 h. Excess-free probe was removed by desalting the reaction mixture into PBS using a VivaSpin protein concentrator (Sartorius Stedim). .. The number of chelators coupled per antibody (c/a) was estimated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by comparison of unreacted HAC–N91C and HAC–DOTA.

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    Sartorius AG vivaspin protein concentrator
    Vivaspin Protein Concentrator, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin protein concentrator/product/Sartorius AG
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vivaspin protein concentrator - by Bioz Stars, 2020-04
    89/100 stars
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