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Sartorius AG vivaspin 20 protein concentrator
Vivaspin 20 Protein Concentrator, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vivaspin 20 protein concentrator/product/Sartorius AG
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
vivaspin 20 protein concentrator - by Bioz Stars, 2020-04
87/100 stars

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30-kda molecular weight cut-off

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Centrifugation:

Article Title: Prevalence and Phase Variable Expression Status of Two Autotransporters, NalP and MspA, in Carriage and Disease Isolates of Neisseria meningitidis
Article Snippet: .. Meningococci were harvested by centrifugation (8,000× g for 10 min) and supernatants filtered (Steritop 0.22 µm pore size; Millipore) and concentrated approximately 200-fold (Vivaspin-20 protein concentrator, 30-kDa molecular weight cut-off; Sartorius). .. Secreted proteins were electrophoretically separated using 10% polyacrylamide mini-gels (Mini-Protean III, Bio-Rad) and transferred to nitrocellulose membranes.

Blocking Assay:

Article Title: Prevalence and Phase Variable Expression Status of Two Autotransporters, NalP and MspA, in Carriage and Disease Isolates of Neisseria meningitidis
Article Snippet: Meningococci were harvested by centrifugation (8,000× g for 10 min) and supernatants filtered (Steritop 0.22 µm pore size; Millipore) and concentrated approximately 200-fold (Vivaspin-20 protein concentrator, 30-kDa molecular weight cut-off; Sartorius). .. Membranes were probed with rabbit anti-MspA or rabbit anti-NalP diluted 1∶4000 or 1∶500, respectively in blocking solution and incubated for 2 h. After washing three times in 1× PBS with 0.1% Tween® 20 (PBST), membranes were incubated for 2 h with goat anti-rabbit IgG-alkaline phosphatase conjugate (Sigma), at a dilution of 1∶30,000 in blocking solution.

Incubation:

Article Title: Subunit Organization of a Synechocystis Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] [W] Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] [W] [OA]
Article Snippet: After an incubation period of 3 h on a rotating wheel, the sample was transferred to a Proteus one-step batch midi spin column (Generon) and washed with washing buffer (50 mM HEPES, pH 7.2, 5% [v/v] glycerol, 100 mM NaCl, 5 mM MgCl2 , 10 µM ZnCl2 , 1 mM DTT, and 0.03% [w/v] ) until the washes were clear. .. The eluted fractions were then pooled and concentrated to 1 mL using a Vivaspin 20 protein concentrator (Sartorius Stedim Biotech) with a 100 kD molecular mass cutoff.

Article Title: Prevalence and Phase Variable Expression Status of Two Autotransporters, NalP and MspA, in Carriage and Disease Isolates of Neisseria meningitidis
Article Snippet: Meningococci were harvested by centrifugation (8,000× g for 10 min) and supernatants filtered (Steritop 0.22 µm pore size; Millipore) and concentrated approximately 200-fold (Vivaspin-20 protein concentrator, 30-kDa molecular weight cut-off; Sartorius). .. Membranes were probed with rabbit anti-MspA or rabbit anti-NalP diluted 1∶4000 or 1∶500, respectively in blocking solution and incubated for 2 h. After washing three times in 1× PBS with 0.1% Tween® 20 (PBST), membranes were incubated for 2 h with goat anti-rabbit IgG-alkaline phosphatase conjugate (Sigma), at a dilution of 1∶30,000 in blocking solution.

Expressing:

Article Title: Prevalence and Phase Variable Expression Status of Two Autotransporters, NalP and MspA, in Carriage and Disease Isolates of Neisseria meningitidis
Article Snippet: Phenotypic analysis Expression of NalP and MspA in representative strains was confirmed by immunoblot analysis of concentrated secreted protein preparations. .. Meningococci were harvested by centrifugation (8,000× g for 10 min) and supernatants filtered (Steritop 0.22 µm pore size; Millipore) and concentrated approximately 200-fold (Vivaspin-20 protein concentrator, 30-kDa molecular weight cut-off; Sartorius).

Modification:

Article Title: Prevalence and Phase Variable Expression Status of Two Autotransporters, NalP and MspA, in Carriage and Disease Isolates of Neisseria meningitidis
Article Snippet: Secreted proteins were prepared from 200 ml cultures of meningococci grown at 37°C overnight in Dulbecco's Modified Eagle Medium (DMEM; Invitrogen). .. Meningococci were harvested by centrifugation (8,000× g for 10 min) and supernatants filtered (Steritop 0.22 µm pore size; Millipore) and concentrated approximately 200-fold (Vivaspin-20 protein concentrator, 30-kDa molecular weight cut-off; Sartorius).

Affinity Purification:

Article Title: Subunit Organization of a Synechocystis Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] [W] Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] [W] [OA]
Article Snippet: Paragraph title: Affinity Purification of FtsH2-GST ... The eluted fractions were then pooled and concentrated to 1 mL using a Vivaspin 20 protein concentrator (Sartorius Stedim Biotech) with a 100 kD molecular mass cutoff.

SDS Page:

Article Title: Subunit Organization of a Synechocystis Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] [W] Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair [C] [W] [OA]
Article Snippet: A last wash was performed with 10 mL washing buffer for one-dimensional SDS-PAGE analysis. .. The eluted fractions were then pooled and concentrated to 1 mL using a Vivaspin 20 protein concentrator (Sartorius Stedim Biotech) with a 100 kD molecular mass cutoff.

Molecular Weight:

Article Title: Prevalence and Phase Variable Expression Status of Two Autotransporters, NalP and MspA, in Carriage and Disease Isolates of Neisseria meningitidis
Article Snippet: .. Meningococci were harvested by centrifugation (8,000× g for 10 min) and supernatants filtered (Steritop 0.22 µm pore size; Millipore) and concentrated approximately 200-fold (Vivaspin-20 protein concentrator, 30-kDa molecular weight cut-off; Sartorius). .. Secreted proteins were electrophoretically separated using 10% polyacrylamide mini-gels (Mini-Protean III, Bio-Rad) and transferred to nitrocellulose membranes.

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    Sartorius AG vivaspin 20 protein concentrator
    Vivaspin 20 Protein Concentrator, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vivaspin 20 protein concentrator/product/Sartorius AG
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vivaspin 20 protein concentrator - by Bioz Stars, 2020-04
    87/100 stars
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