vitox vitamin supplement  (Thermo Fisher)


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    Thermo Fisher vitox vitamin supplement
    Vitox Vitamin Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vitox vitamin supplement/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vitox vitamin supplement - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    GGT Activity Assay:

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL). .. The isogenic ggt mutants were verified by a GGT activity assay [ ], PCR and nucleotide sequencing.

    Amplification:

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: The resultant plasmid was amplified in XL1-Blue MRF′ E. coli (Agilent Technologies) and used as a suicide plasmid in H. pylori SS1 and PMSS1 (a kind gift from Sara Lindén and Anne Muller, respectively). .. Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    Mass Spectrometry:

    Article Title: Development and evaluation of a culture-free microbiota profiling platform (MYcrobiota) for clinical diagnostics
    Article Snippet: The routine bacterial culture methods included a 48-h incubation at 35 °C on tryptic soy agar plates with 5% sheep blood (TSASB, Oxoid), colistin aztreonam blood agar plates (CAP, Oxoid), and cystine lactose electrolyte deficient agar plates (CLED, Oxoid) under aerobic conditions; a 48-h incubation at 35 °C on chocolate agar with Vitox supplement (CHOCV, Oxoid) under 5% CO2 conditions; and a 48-h incubation at 35 °C on TSASB under anaerobic conditions. .. All Gram-negative rods, beta-hemolytic streptococci, Staphylococcus aureus , Staphylococcus lugdunensis , and anaerobic bacteria cultured were reported as potentially relevant bacteria, of which the identification of aerobic bacteria was obtained using MALDI-TOF mass spectrometry (Bruker).

    Incubation:

    Article Title: In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
    Article Snippet: The biphasic medium consisted of Brucella agar (BD, Franklin Lakes, New Jersey, USA) supplemented with 20% fetal calf serum (GE Healthcare Life Sciences, Logan, USA), 5 mg amphotericin B/l (Sigma-Aldrich, Saint Louis, Missouri, USA), Campylobacter selective supplement (Oxoid) and Vitox supplement (Oxoid). .. After incubation, the bacteria were harvested and the concentration was determined using an improved Neubauer counting chamber (Sigma-Aldrich).

    Article Title: Development and evaluation of a culture-free microbiota profiling platform (MYcrobiota) for clinical diagnostics
    Article Snippet: .. The routine bacterial culture methods included a 48-h incubation at 35 °C on tryptic soy agar plates with 5% sheep blood (TSASB, Oxoid), colistin aztreonam blood agar plates (CAP, Oxoid), and cystine lactose electrolyte deficient agar plates (CLED, Oxoid) under aerobic conditions; a 48-h incubation at 35 °C on chocolate agar with Vitox supplement (CHOCV, Oxoid) under 5% CO2 conditions; and a 48-h incubation at 35 °C on TSASB under anaerobic conditions. .. All Gram-negative rods, beta-hemolytic streptococci, Staphylococcus aureus , Staphylococcus lugdunensis , and anaerobic bacteria cultured were reported as potentially relevant bacteria, of which the identification of aerobic bacteria was obtained using MALDI-TOF mass spectrometry (Bruker).

    Expressing:

    Article Title: Evidence for Conserved Function of ?-Glutamyltranspeptidase in Helicobacter Genus
    Article Snippet: .. RNA extraction and RT-PCR To analyse the expression of both bgh1 and bgh2 during growth, H. bilis wild-type CCUG 23435 was cultivated in Brain Heart Infusion (BHI; BD, NJ USA) containing 20% FBS and Vitox supplement (Oxoid Ltd., Cambridge, UK) at 37°C microaerobically by continuous shaking at 150 rpm. .. OD600 was measured at 8, 16 and 24 h and the same amount of cells was treated with RNAprotect Bacteria Reagent (Qiagen GmbH, Hamburg Germany).

    Modification:

    Article Title: Genotyping of Francisella tularensis Strains by Pulsed-Field Gel Electrophoresis, Amplified Fragment Length Polymorphism Fingerprinting, and 16S rRNA Gene Sequencing
    Article Snippet: .. All isolates, which had been stored frozen at −80°C, were grown aerobically at 37°C for 2 days on modified Thayer-Martin agar plates containing GC medium base (36 g/liter), hemoglobin (10 g/liter), and Vitox supplement (2 vials/liter; Oxoid Ltd., Basingstoke, England). .. Sequencing of the 16S rRNA genes was performed with universal primers directed at a 1.4-kb conserved region of the gene ( ).

    Transformation Assay:

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: The H. pylori SS1 ggt mutant (SS1Δggt ) and H. pylori PMSS1 ggt mutant (PMSS1Δggt ) were obtained by electrotransformation [ ] or natural transformation [ ] as described previously. .. Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    Hybridization:

    Article Title: Deletion of porA by Recombination between Clusters of Repetitive Extragenic Palindromic Sequences in Neisseria meningitidis
    Article Snippet: Bacteria were grown on a GC agar base (Difco Laboratories, Detroit, Mich.) containing 1% Vitox supplement (Oxoid Laboratories, Ltd., Basingstoke, United Kingdom) at 37°C in a humidified atmosphere of 5% CO2 in air. .. Aliquots of the diluted culture were plated on GC agar plates and assessed for the presence of PorA- and porA -negative variants by colony immunoblotting and Southern colony hybridization, respectively.

    Inverse PCR:

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: Very briefly, deletion of ggt in H. pylori SS1 and PMSS1 was introduced by allelic exchange using pBluescript II SK (+) phagemid vector (Agilent Technologies, California, USA) in which ~440 bp of the 5′ –end and ~430 bp of the 3′ –end of the target gene and the kanamycin resistance cassette from plasmid pKD4 [ ] were ligated through a PCR-mediated strategy with 2 cycles of inverse PCR and fusion PCR [ ]. .. Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    Cell Culture:

    Article Title: Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes
    Article Snippet: .. Helicobacter suis culture conditions Helicobacter suis strain HS1 and HS5 were cultured in biphasic Brucella culture plates (BD BBL, 211086) containing 20% fetal bovine serum (FBS) (HyClone, SH30071.01), Vitox supplement (Oxoid, SR0090H), Skirrow Campylobacter selective supplement (Oxoid, SR0069E), and 5 mg/L amphotericin B. ..

    Article Title: Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter suis
    Article Snippet: Paragraph title: H. suis isolates cultured in vitro and DNA extraction. ... Bacteria were grown microaerobically (85% N2 , 10% CO2 , 5% O2 ; 37°C; 48 to 96 h) on biphasic brucella (Becton Dickinson, Franklin Lakes, NJ) culture plates (with a pH adjusted to 5) supplemented with 20% fetal calf serum (HyClone, Logan, UT) and Vitox supplement (Oxoid), as described previously ( ).

    Article Title: Development and evaluation of a culture-free microbiota profiling platform (MYcrobiota) for clinical diagnostics
    Article Snippet: All samples were cultured according to standard laboratory protocols performed in our laboratory and stored at − 80 °C for subsequent MYcrobiota analysis. .. The routine bacterial culture methods included a 48-h incubation at 35 °C on tryptic soy agar plates with 5% sheep blood (TSASB, Oxoid), colistin aztreonam blood agar plates (CAP, Oxoid), and cystine lactose electrolyte deficient agar plates (CLED, Oxoid) under aerobic conditions; a 48-h incubation at 35 °C on chocolate agar with Vitox supplement (CHOCV, Oxoid) under 5% CO2 conditions; and a 48-h incubation at 35 °C on TSASB under anaerobic conditions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Evidence for Conserved Function of ?-Glutamyltranspeptidase in Helicobacter Genus
    Article Snippet: .. RNA extraction and RT-PCR To analyse the expression of both bgh1 and bgh2 during growth, H. bilis wild-type CCUG 23435 was cultivated in Brain Heart Infusion (BHI; BD, NJ USA) containing 20% FBS and Vitox supplement (Oxoid Ltd., Cambridge, UK) at 37°C microaerobically by continuous shaking at 150 rpm. .. OD600 was measured at 8, 16 and 24 h and the same amount of cells was treated with RNAprotect Bacteria Reagent (Qiagen GmbH, Hamburg Germany).

    other:

    Article Title: Sialylation of Helicobacter bizzozeronii lipopolysaccharides modulates Toll-like receptor (TLR) 2 mediated response
    Article Snippet: For LPS extraction Helicobacter strains were cultivated in Brain Heart Infusion (BHI, BD, Becton, Dickinson and Co., NJ, USA) containing 10% of FBS, Skirrow selective supplement (Oxoid) and Vitox supplement (Oxoid) at 37 °C in a jar with microaerobic atmosphere.

    Article Title: Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates
    Article Snippet: All bacteria were grown overnight at 37°C on sheep blood agar plates, except for Actinobacillus pleuropneumoniae and Haemophilus influenzae , which were grown on 8% sheep blood chocolate agar with a Vitox supplement (Oxoid) overnight at 37°C in 5% CO2 .

    Polymerase Chain Reaction:

    Article Title: Multiplex PCR Assay for Differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis
    Article Snippet: Strains were grown on brain heart infusion agar (Oxoid, Basingstoke, England) containing 10% horse blood, 5 mg of amphotericin B/liter, Campylobacter selective supplement (Skirrow [Oxoid] containing 10 mg of vancomycin, 5 mg of trimethoprim lactate, and 2,500 U of polymyxin B/liter), and Vitox supplement (Oxoid). .. Twenty-three other strains belonging to different Helicobacter and Campylobacter species and some related taxa were used to determine the specificity of the multiplex PCR assay and are listed in Table .

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: All primers used for PCR-mediated construction of the recombinant plasmids are shown in Table . .. Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    Recombinant:

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: All primers used for PCR-mediated construction of the recombinant plasmids are shown in Table . .. Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    DNA Extraction:

    Article Title: Deletion of porA by Recombination between Clusters of Repetitive Extragenic Palindromic Sequences in Neisseria meningitidis
    Article Snippet: Paragraph title: Strains, culture conditions, and chromosomal DNA isolation. ... Bacteria were grown on a GC agar base (Difco Laboratories, Detroit, Mich.) containing 1% Vitox supplement (Oxoid Laboratories, Ltd., Basingstoke, United Kingdom) at 37°C in a humidified atmosphere of 5% CO2 in air.

    Article Title: Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter suis
    Article Snippet: Paragraph title: H. suis isolates cultured in vitro and DNA extraction. ... Bacteria were grown microaerobically (85% N2 , 10% CO2 , 5% O2 ; 37°C; 48 to 96 h) on biphasic brucella (Becton Dickinson, Franklin Lakes, NJ) culture plates (with a pH adjusted to 5) supplemented with 20% fetal calf serum (HyClone, Logan, UT) and Vitox supplement (Oxoid), as described previously ( ).

    Mutagenesis:

    Article Title: Evidence for Conserved Function of ?-Glutamyltranspeptidase in Helicobacter Genus
    Article Snippet: RNA extraction and RT-PCR To analyse the expression of both bgh1 and bgh2 during growth, H. bilis wild-type CCUG 23435 was cultivated in Brain Heart Infusion (BHI; BD, NJ USA) containing 20% FBS and Vitox supplement (Oxoid Ltd., Cambridge, UK) at 37°C microaerobically by continuous shaking at 150 rpm. .. Moreover, H. bilis CCUG 23435 and the mutant MR9 were cultivated in YT×2 medium (16 g/L Tryptone, 10 g/L Yeast Extract, 5 g/L NaCl) containing 10% FBS at 37°C in a microaerobic atmosphere.

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: Paragraph title: Construction of isogenic ggt mutant strains of H. suis and H. pylori ... Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    Isolation:

    Article Title: Helicobacter suis Causes Severe Gastric Pathology in Mouse and Mongolian Gerbil Models of Human Gastric Disease
    Article Snippet: H. suis strain HS5 was isolated from the gastric mucosa of a sow as described previously . .. Bacteria were grown under microaerobic conditions (85% N2 , 10% CO2 , 5% O2 ; 37°C; 72 h) on biphasic Brucella (Oxoid, Basingstoke, UK) culture plates supplemented with 20% fetal calf serum (HyClone, Logan, UT, USA) and Vitox supplement (Oxoid) .

    Article Title: In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
    Article Snippet: H. suis strains Helicobacter suis strains HS1, HS8 and P13/26 were isolated from the gastric mucosa of pigs from different herds according to the method described by Baele et al. [ ]. .. The biphasic medium consisted of Brucella agar (BD, Franklin Lakes, New Jersey, USA) supplemented with 20% fetal calf serum (GE Healthcare Life Sciences, Logan, USA), 5 mg amphotericin B/l (Sigma-Aldrich, Saint Louis, Missouri, USA), Campylobacter selective supplement (Oxoid) and Vitox supplement (Oxoid).

    Article Title: Helicobacter suis affects the health and function of porcine gastric parietal cells
    Article Snippet: Preparation of H. suis and bacterial lysate H. suis strain HS5cLP was grown on Brucella agar (BD, Franklin Lakes, NJ, USA) plates with a pH of 5 and supplemented with 20% fetal calf serum (HyClone), 5 mg/L amphotericin B (Fungizone; Bristol-Myers Squibb, Epernon, France), Campylobacter selective supplement (Oxoid, Basingstoke, UK) and Vitox supplement (Oxoid) under microaerobic and biphasic conditions (37 °C; 85% N2 , 10% CO2 , 5% O2 ) as described elsewhere [ ]. .. This strain was isolated in 2008 from the stomach of a slaughterhouse pig [ ].

    Article Title: Multiplex PCR Assay for Differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis
    Article Snippet: Fifteen reference strains (Table ) belonging to the species Helicobacter felis , H. bizzozeronii , and H. salomonis and isolated from cats and dogs were used for the development of species-specific primers. .. Strains were grown on brain heart infusion agar (Oxoid, Basingstoke, England) containing 10% horse blood, 5 mg of amphotericin B/liter, Campylobacter selective supplement (Skirrow [Oxoid] containing 10 mg of vancomycin, 5 mg of trimethoprim lactate, and 2,500 U of polymyxin B/liter), and Vitox supplement (Oxoid).

    Article Title: Deletion of porA by Recombination between Clusters of Repetitive Extragenic Palindromic Sequences in Neisseria meningitidis
    Article Snippet: The latter two strains were isolated from patients with meningococcal disease during the epidemic period in Norway in the 1970s and are now used as reference strains. .. Bacteria were grown on a GC agar base (Difco Laboratories, Detroit, Mich.) containing 1% Vitox supplement (Oxoid Laboratories, Ltd., Basingstoke, United Kingdom) at 37°C in a humidified atmosphere of 5% CO2 in air.

    Article Title: Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter suis
    Article Snippet: H. suis strains HS1 to HS10 were isolated from the gastric mucosa of sows as described previously ( ). .. Bacteria were grown microaerobically (85% N2 , 10% CO2 , 5% O2 ; 37°C; 48 to 96 h) on biphasic brucella (Becton Dickinson, Franklin Lakes, NJ) culture plates (with a pH adjusted to 5) supplemented with 20% fetal calf serum (HyClone, Logan, UT) and Vitox supplement (Oxoid), as described previously ( ).

    RNA Extraction:

    Article Title: Evidence for Conserved Function of ?-Glutamyltranspeptidase in Helicobacter Genus
    Article Snippet: .. RNA extraction and RT-PCR To analyse the expression of both bgh1 and bgh2 during growth, H. bilis wild-type CCUG 23435 was cultivated in Brain Heart Infusion (BHI; BD, NJ USA) containing 20% FBS and Vitox supplement (Oxoid Ltd., Cambridge, UK) at 37°C microaerobically by continuous shaking at 150 rpm. .. OD600 was measured at 8, 16 and 24 h and the same amount of cells was treated with RNAprotect Bacteria Reagent (Qiagen GmbH, Hamburg Germany).

    Mouse Assay:

    Article Title: Helicobacter suis Causes Severe Gastric Pathology in Mouse and Mongolian Gerbil Models of Human Gastric Disease
    Article Snippet: Animal and bacterial strains Twenty-seven specific-pathogen-free (SPF) female six-week-old mice of each of two strains (BALB/c and C57BL/6) were purchased from Harlan NL (Horst, The Netherlands). .. Bacteria were grown under microaerobic conditions (85% N2 , 10% CO2 , 5% O2 ; 37°C; 72 h) on biphasic Brucella (Oxoid, Basingstoke, UK) culture plates supplemented with 20% fetal calf serum (HyClone, Logan, UT, USA) and Vitox supplement (Oxoid) .

    Sequencing:

    Article Title: Significant Reduction in Helicobacter pylori Load in Humans with Non-viable Lactobacillus reuteri DSM17648: A Pilot Study
    Article Snippet: Other Helicobacter species were grown in Brucella/FBS broth containing additionally 0.75 % (v/v) Vitox supplement (Oxoid, Wesel, Germany) [ ]. .. The taxonomic identification of the Lactobacillus strains to the species level relied on 16 S-rDNA sequence analysis (sequencing done LCG Genomics, taxonomic classification done by Nadicom, Karlsruhe, Germany) using the primers 27f (5′-AGAGTTTGATCMTGGCTCAG-3′) and 1492r (5′-ACGGYTACCTTGTTACGACTT-3′) [ ] and on phenotypic characterization using the API 50 CH system and apiweb™ software (bioMerieux, France).

    Article Title: In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
    Article Snippet: The strains were shown to be genetically different by multilocus sequence typing [ ]. .. The biphasic medium consisted of Brucella agar (BD, Franklin Lakes, New Jersey, USA) supplemented with 20% fetal calf serum (GE Healthcare Life Sciences, Logan, USA), 5 mg amphotericin B/l (Sigma-Aldrich, Saint Louis, Missouri, USA), Campylobacter selective supplement (Oxoid) and Vitox supplement (Oxoid).

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL). .. The isogenic ggt mutants were verified by a GGT activity assay [ ], PCR and nucleotide sequencing.

    Plasmid Preparation:

    Article Title: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections
    Article Snippet: The resultant plasmid was amplified in XL1-Blue MRF′ E. coli (Agilent Technologies) and used as a suicide plasmid in H. pylori SS1 and PMSS1 (a kind gift from Sara Lindén and Anne Muller, respectively). .. Finally, bacteria were selected on columbia agar plates (Oxoid, Basingstoke, UK) with Vitox supplement (Oxoid), 5% (v/v) defibrinated sheep blood (E & O Laboratories Ltd, Bonnybridge, UK), and kanamycin (25 μg/mL).

    Software:

    Article Title: Significant Reduction in Helicobacter pylori Load in Humans with Non-viable Lactobacillus reuteri DSM17648: A Pilot Study
    Article Snippet: Other Helicobacter species were grown in Brucella/FBS broth containing additionally 0.75 % (v/v) Vitox supplement (Oxoid, Wesel, Germany) [ ]. .. The taxonomic identification of the Lactobacillus strains to the species level relied on 16 S-rDNA sequence analysis (sequencing done LCG Genomics, taxonomic classification done by Nadicom, Karlsruhe, Germany) using the primers 27f (5′-AGAGTTTGATCMTGGCTCAG-3′) and 1492r (5′-ACGGYTACCTTGTTACGACTT-3′) [ ] and on phenotypic characterization using the API 50 CH system and apiweb™ software (bioMerieux, France).

    Multiplex Assay:

    Article Title: Multiplex PCR Assay for Differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis
    Article Snippet: Strains were grown on brain heart infusion agar (Oxoid, Basingstoke, England) containing 10% horse blood, 5 mg of amphotericin B/liter, Campylobacter selective supplement (Skirrow [Oxoid] containing 10 mg of vancomycin, 5 mg of trimethoprim lactate, and 2,500 U of polymyxin B/liter), and Vitox supplement (Oxoid). .. Twenty-three other strains belonging to different Helicobacter and Campylobacter species and some related taxa were used to determine the specificity of the multiplex PCR assay and are listed in Table .

    In Vitro:

    Article Title: Deletion of porA by Recombination between Clusters of Repetitive Extragenic Palindromic Sequences in Neisseria meningitidis
    Article Snippet: In addition, nine isolates, strains 890456 (B:16:P1.5), 900545 (B:4:P1.15), 900111 (B:15:P1.16), 2996 (B:2b:P1.2), 900181 (B:2b:P1.2), 900619 (B:2b:P1.2), 901569 (C:2a:P1.2), H44/76 (B:15:P1.7,16), and H355 (B:15:P1.15), from the collection of the RLBM were used for in vitro studies. .. Bacteria were grown on a GC agar base (Difco Laboratories, Detroit, Mich.) containing 1% Vitox supplement (Oxoid Laboratories, Ltd., Basingstoke, United Kingdom) at 37°C in a humidified atmosphere of 5% CO2 in air.

    Article Title: Multilocus Sequence Typing of the Porcine and Human Gastric Pathogen Helicobacter suis
    Article Snippet: Paragraph title: H. suis isolates cultured in vitro and DNA extraction. ... Bacteria were grown microaerobically (85% N2 , 10% CO2 , 5% O2 ; 37°C; 48 to 96 h) on biphasic brucella (Becton Dickinson, Franklin Lakes, NJ) culture plates (with a pH adjusted to 5) supplemented with 20% fetal calf serum (HyClone, Logan, UT) and Vitox supplement (Oxoid), as described previously ( ).

    Concentration Assay:

    Article Title: Helicobacter suis Causes Severe Gastric Pathology in Mouse and Mongolian Gerbil Models of Human Gastric Disease
    Article Snippet: Bacteria were grown under microaerobic conditions (85% N2 , 10% CO2 , 5% O2 ; 37°C; 72 h) on biphasic Brucella (Oxoid, Basingstoke, UK) culture plates supplemented with 20% fetal calf serum (HyClone, Logan, UT, USA) and Vitox supplement (Oxoid) . .. The bacteria were harvested and the final concentration was adjusted to 2×108 viable bacteria/ml, as determined by counting in an improved Neubauer counting chamber.

    Article Title: In-feed bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
    Article Snippet: The biphasic medium consisted of Brucella agar (BD, Franklin Lakes, New Jersey, USA) supplemented with 20% fetal calf serum (GE Healthcare Life Sciences, Logan, USA), 5 mg amphotericin B/l (Sigma-Aldrich, Saint Louis, Missouri, USA), Campylobacter selective supplement (Oxoid) and Vitox supplement (Oxoid). .. After incubation, the bacteria were harvested and the concentration was determined using an improved Neubauer counting chamber (Sigma-Aldrich).

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    Thermo Fisher haemophilus selective agar
    Haemophilus Selective Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haemophilus selective agar/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    haemophilus selective agar - by Bioz Stars, 2020-04
    99/100 stars
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