viral rneasy minikit  (Qiagen)


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    Structured Review

    Qiagen viral rneasy minikit
    Viral Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral rneasy minikit/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    viral rneasy minikit - by Bioz Stars, 2020-04
    90/100 stars

    Related Products / Commonly Used Together

    phosphate-buffered saline pbs
    rna extraction
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    Related Articles

    Centrifugation:

    Article Title: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
    Article Snippet: .. After centrifugation at 9,000 g at 4°C for 5 min, the supernatant was used for RNA isolation with the RNeasy Mini Kit, according to the manufacturer’s recommendations. .. Optimized method for RNA isolation from sessile cells using the RNeasy Mini Kit To improve the quality and quantity of the total RNA that was obtained from sessile cells of S. aureus , we modified the protocol for RNA preparation by using sheared whole-cell lysate coupled to RNA isolation using the RNeasy Mini Kit.

    Amplification:

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany). .. For amplification, a primer pair specific for human RAGE was used.

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. PCR amplification (in a 20 μ L scale) was performed with a heat‐resistant DNA polymerase (PrimeSTAR GXL, Takara) in a thermal cycler (Biometra, Gottingen, Germany) according to the following protocol;denaturation at 94°C for 2 min followed by 17–35 cycles of: denaturation (94°C, 10s), annealing (60°C, 10s), and extension (72°C, 30s).

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction. .. The amplification conditions on the PE Applied Biosystems 7700 sequence detector were the following: 2 min at 50°C, 10 min at 95°C, 40 cycles of 95°C for 15 s and 60°C for 1 min for all reactions.

    Article Title: Comparison of RNA extraction methods to augment the sensitivity for the differentiation of vesicular stomatitis virus Indiana1 and New Jersey
    Article Snippet: However, VSV‐NJ was detected as low as 0.1 fg of total RNA when only one primer set for the amplification of L gene of VSV‐NJ was used in the assay (Fig. ). .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples.

    Filtration:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Synthesized:

    Article Title: Amino Acids as Signaling Molecules Modulating Bone Turnover
    Article Snippet: .. Total RNA was extracted from BMSC cells with the RNeasy mini kit (Qiagen). cDNA was synthesized using 1 μg total RNA (SuperScript III First-Strand Synthesis System, Invitrogen). .. For PCR reactions the “Platinum PCR SuperMix” (Invitrogen) was used (94°C, 30 sec; 57° C, 30 sec; 72° C, 1 min for 35 cycles).

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany). .. The cDNA was synthesized from total RNA with Primerscript RT reagent kit with gDNA Eraser (TAKALA DRR047A, Dalian, China).

    Article Title: NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B
    Article Snippet: RNA extraction and qRT-PCR RNeasy Mini Kit (Qiagen Hilden, Germany) was purchased for RNA extraction. .. In brief, cDNA was synthesized by using the i-Script™ Reverse Transcription Supermix (Biorad, Hercules, CA, United States) from 1 µg of total RNA.

    Quantitative RT-PCR:

    Article Title: A preliminary analysis of hepatitis C virus in pancreatic islet cells
    Article Snippet: mRNA expression of HCV entry factors Total RNA from 3 different human islets donors was isolated using TRIzol reagent (Thermo Scientific) in combination with the RNeasy Mini kit (Qiagen) followed by DNase treatment. .. Five hundred nanograms of total RNA were retrotranscribed using the Superscript III kit (Thermo Scientific), and the cDNAs obtained were utilized as templates for quantitative real-time RT-PCR analysis of CD81 (72 base pair [bp]), occludin (63 bp), claudin-1 (101 bp), and SR-B1 (50 bp), as well as the housekeeping gene GAPDH (94 bp). cDNA was run on an AbiPRISM 7300 fast real-time cycler using the power SYBR Green real-time PCR master mix kit and quantified by built-in SYBR Green Analysis.

    Article Title: NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B
    Article Snippet: .. RNA extraction and qRT-PCR RNeasy Mini Kit (Qiagen Hilden, Germany) was purchased for RNA extraction. .. In brief, cDNA was synthesized by using the i-Script™ Reverse Transcription Supermix (Biorad, Hercules, CA, United States) from 1 µg of total RNA.

    SYBR Green Assay:

    Article Title: A preliminary analysis of hepatitis C virus in pancreatic islet cells
    Article Snippet: mRNA expression of HCV entry factors Total RNA from 3 different human islets donors was isolated using TRIzol reagent (Thermo Scientific) in combination with the RNeasy Mini kit (Qiagen) followed by DNase treatment. .. Five hundred nanograms of total RNA were retrotranscribed using the Superscript III kit (Thermo Scientific), and the cDNAs obtained were utilized as templates for quantitative real-time RT-PCR analysis of CD81 (72 base pair [bp]), occludin (63 bp), claudin-1 (101 bp), and SR-B1 (50 bp), as well as the housekeeping gene GAPDH (94 bp). cDNA was run on an AbiPRISM 7300 fast real-time cycler using the power SYBR Green real-time PCR master mix kit and quantified by built-in SYBR Green Analysis.

    Incubation:

    Article Title: Bias in recent miRBase annotations potentially associated with RNA quality issues
    Article Snippet: In detail, frozen tissue was homogenized in 700 μl QIAzol Lysis Reagent with using Tissue Lyser (Qiagen, Hilden, Germany) and incubated at room temperature for 5 min. After addition of 200 µl chloroform, samples were centrifuged at 12,000 rpm at 4 °C for phase separation. .. For determination of non-miRNA mediated background due to potential contamination with fragments of other RNAs during RNA degradation, total RNA was isolated from samples of three animals using RNeasy mini Kit (Qiagen, Hilden, Germany) with on column DNase digestion.

    Expressing:

    Article Title: A preliminary analysis of hepatitis C virus in pancreatic islet cells
    Article Snippet: .. mRNA expression of HCV entry factors Total RNA from 3 different human islets donors was isolated using TRIzol reagent (Thermo Scientific) in combination with the RNeasy Mini kit (Qiagen) followed by DNase treatment. .. Five hundred nanograms of total RNA were retrotranscribed using the Superscript III kit (Thermo Scientific), and the cDNAs obtained were utilized as templates for quantitative real-time RT-PCR analysis of CD81 (72 base pair [bp]), occludin (63 bp), claudin-1 (101 bp), and SR-B1 (50 bp), as well as the housekeeping gene GAPDH (94 bp). cDNA was run on an AbiPRISM 7300 fast real-time cycler using the power SYBR Green real-time PCR master mix kit and quantified by built-in SYBR Green Analysis.

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Paragraph title: Analysis of mRNA Expression ... Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction.

    Transfection:

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: The effect of siRNA on the RNA expression of RAGE was assessed by real-time quantitative PCR after 48 hr of transfection. .. Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Amino Acids as Signaling Molecules Modulating Bone Turnover
    Article Snippet: Paragraph title: RT-PCR for amino acid sensors ... Total RNA was extracted from BMSC cells with the RNeasy mini kit (Qiagen). cDNA was synthesized using 1 μg total RNA (SuperScript III First-Strand Synthesis System, Invitrogen).

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: .. Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. PCR amplification (in a 20 μ L scale) was performed with a heat‐resistant DNA polymerase (PrimeSTAR GXL, Takara) in a thermal cycler (Biometra, Gottingen, Germany) according to the following protocol;denaturation at 94°C for 2 min followed by 17–35 cycles of: denaturation (94°C, 10s), annealing (60°C, 10s), and extension (72°C, 30s).

    Article Title: Comparison of RNA extraction methods to augment the sensitivity for the differentiation of vesicular stomatitis virus Indiana1 and New Jersey
    Article Snippet: .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples. .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples.

    other:

    Article Title: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
    Article Snippet: Therefore, we designed a simple optimized protocol using the RNeasy Mini Kit that ensures high-quality RNA preparations from planktonic and sessile cells of MRSA, compatible with experiments that require integrity and good quantity of this molecule.

    Polymerase Chain Reaction:

    Article Title: Amino Acids as Signaling Molecules Modulating Bone Turnover
    Article Snippet: Total RNA was extracted from BMSC cells with the RNeasy mini kit (Qiagen). cDNA was synthesized using 1 μg total RNA (SuperScript III First-Strand Synthesis System, Invitrogen). .. For PCR reactions the “Platinum PCR SuperMix” (Invitrogen) was used (94°C, 30 sec; 57° C, 30 sec; 72° C, 1 min for 35 cycles).

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. PCR amplification (in a 20 μ L scale) was performed with a heat‐resistant DNA polymerase (PrimeSTAR GXL, Takara) in a thermal cycler (Biometra, Gottingen, Germany) according to the following protocol;denaturation at 94°C for 2 min followed by 17–35 cycles of: denaturation (94°C, 10s), annealing (60°C, 10s), and extension (72°C, 30s).

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction. .. Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector.

    Molecular Weight:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Fluorescence:

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: HCAECs transplanted with Cy3-labeled siRNA were detected by fluorescence microscopy after 6 hr of transfection. .. Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany).

    Isolation:

    Article Title: Comparison of RNA Isolation Methods From Insect Larvae
    Article Snippet: .. Four methods including three commercial kits RNeasy Mini Kit (Qiagen), SV Total RNA isolation system (Promega), TRIzol reagent (Invitrogen), and a cetyl trimethylammonium bromide (CTAB)-based method were compared regarding their ability to isolate RNA from whole-body larvae ofThaumatotibia leucotreta (Meyrick),Thanatophilus micans (F.),Plutella xylostella (L.), andTenebrio molitor (L.). ..

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: .. Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany). .. The cDNA was synthesized from total RNA with Primerscript RT reagent kit with gDNA Eraser (TAKALA DRR047A, Dalian, China).

    Article Title: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
    Article Snippet: .. After centrifugation at 9,000 g at 4°C for 5 min, the supernatant was used for RNA isolation with the RNeasy Mini Kit, according to the manufacturer’s recommendations. .. Optimized method for RNA isolation from sessile cells using the RNeasy Mini Kit To improve the quality and quantity of the total RNA that was obtained from sessile cells of S. aureus , we modified the protocol for RNA preparation by using sheared whole-cell lysate coupled to RNA isolation using the RNeasy Mini Kit.

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Article Title: Bias in recent miRBase annotations potentially associated with RNA quality issues
    Article Snippet: .. For determination of non-miRNA mediated background due to potential contamination with fragments of other RNAs during RNA degradation, total RNA was isolated from samples of three animals using RNeasy mini Kit (Qiagen, Hilden, Germany) with on column DNase digestion. .. We denominate this RNA as “RNA depleted of small RNAs”, being aware, that they are not 100% free of any miRNA or small RNA fractions, but the abundance of small RNAs in these samples is greatly reduced in comparison to the isolation procedure using the miRNeasy Kit.

    Article Title: A preliminary analysis of hepatitis C virus in pancreatic islet cells
    Article Snippet: .. mRNA expression of HCV entry factors Total RNA from 3 different human islets donors was isolated using TRIzol reagent (Thermo Scientific) in combination with the RNeasy Mini kit (Qiagen) followed by DNase treatment. .. Five hundred nanograms of total RNA were retrotranscribed using the Superscript III kit (Thermo Scientific), and the cDNAs obtained were utilized as templates for quantitative real-time RT-PCR analysis of CD81 (72 base pair [bp]), occludin (63 bp), claudin-1 (101 bp), and SR-B1 (50 bp), as well as the housekeeping gene GAPDH (94 bp). cDNA was run on an AbiPRISM 7300 fast real-time cycler using the power SYBR Green real-time PCR master mix kit and quantified by built-in SYBR Green Analysis.

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: .. Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction. .. For cDNA synthesis, Taqman reverse transcription reagents were used as described in the manufacture’s protocol (PE Applied Biosystems, Foster City, CA, USA).

    Article Title: Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens
    Article Snippet: .. RNA was isolated from each ExoQuick prepared sample using the RNeasy Mini Kit combined with TRIzol LS. ..

    Size-exclusion Chromatography:

    Article Title: Amino Acids as Signaling Molecules Modulating Bone Turnover
    Article Snippet: Total RNA was extracted from BMSC cells with the RNeasy mini kit (Qiagen). cDNA was synthesized using 1 μg total RNA (SuperScript III First-Strand Synthesis System, Invitrogen). .. For PCR reactions the “Platinum PCR SuperMix” (Invitrogen) was used (94°C, 30 sec; 57° C, 30 sec; 72° C, 1 min for 35 cycles).

    Microscopy:

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: HCAECs transplanted with Cy3-labeled siRNA were detected by fluorescence microscopy after 6 hr of transfection. .. Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany).

    Purification:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Article Title: Bias in recent miRBase annotations potentially associated with RNA quality issues
    Article Snippet: Column purification was performed according to the manufacturer protocol without the optional DNAse treatment, and RNA was eluted in 50 µl RNase free water. .. For determination of non-miRNA mediated background due to potential contamination with fragments of other RNAs during RNA degradation, total RNA was isolated from samples of three animals using RNeasy mini Kit (Qiagen, Hilden, Germany) with on column DNase digestion.

    Article Title: Quality Control of RNA Preservation and Extraction from Paraffin-Embedded Tissue: Implications for RT-PCR and Microarray Analysis
    Article Snippet: .. RNA Purification and Complementary DNA (cDNA) Synthesis Where indicated, the total RNA obtained by Trizol extraction was purified by processing with RNeasy Mini Kit (Qiagen) or RNeasy miRNA Mini Kit (Qiagen) according to manufactureŕs instructions. .. After extraction and purification, the RNA yield and purity was determined by measuring absorbance at 260 nm/280 nm on a Nanodrop spectrophotometer (Thermo Fisher Scientific).

    Article Title: Comparison of RNA extraction methods to augment the sensitivity for the differentiation of vesicular stomatitis virus Indiana1 and New Jersey
    Article Snippet: The extraction and purification of RNA by using Qiagen RNeasy mini kit is very rapid, less time consuming (less than one hour) and requires less expertise. .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples.

    Sequencing:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction. .. Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector.

    RNA Extraction:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Article Title: NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B
    Article Snippet: .. RNA extraction and qRT-PCR RNeasy Mini Kit (Qiagen Hilden, Germany) was purchased for RNA extraction. .. In brief, cDNA was synthesized by using the i-Script™ Reverse Transcription Supermix (Biorad, Hercules, CA, United States) from 1 µg of total RNA.

    Article Title: RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells
    Article Snippet: .. RNA extraction from peptide hydrogels Attempts were made to extract RNA from cells using two commercial kits: one solution-based, TRI Reagent® (Sigma-Aldrich), and one column-based, RNeasy Mini Kit® (Qiagen). ..

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: .. Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. PCR amplification (in a 20 μ L scale) was performed with a heat‐resistant DNA polymerase (PrimeSTAR GXL, Takara) in a thermal cycler (Biometra, Gottingen, Germany) according to the following protocol;denaturation at 94°C for 2 min followed by 17–35 cycles of: denaturation (94°C, 10s), annealing (60°C, 10s), and extension (72°C, 30s).

    Software:

    Article Title: Quality Control of RNA Preservation and Extraction from Paraffin-Embedded Tissue: Implications for RT-PCR and Microarray Analysis
    Article Snippet: RNA Purification and Complementary DNA (cDNA) Synthesis Where indicated, the total RNA obtained by Trizol extraction was purified by processing with RNeasy Mini Kit (Qiagen) or RNeasy miRNA Mini Kit (Qiagen) according to manufactureŕs instructions. .. Electropherograms were analysed with Agilent 2100 expert software.

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. The densitometry analysis of electrophoresed PCR amplicons was performed using the ImageJ software (Public domain, NIH, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: The effect of siRNA on the RNA expression of RAGE was assessed by real-time quantitative PCR after 48 hr of transfection. .. Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany).

    Article Title: A preliminary analysis of hepatitis C virus in pancreatic islet cells
    Article Snippet: mRNA expression of HCV entry factors Total RNA from 3 different human islets donors was isolated using TRIzol reagent (Thermo Scientific) in combination with the RNeasy Mini kit (Qiagen) followed by DNase treatment. .. Five hundred nanograms of total RNA were retrotranscribed using the Superscript III kit (Thermo Scientific), and the cDNAs obtained were utilized as templates for quantitative real-time RT-PCR analysis of CD81 (72 base pair [bp]), occludin (63 bp), claudin-1 (101 bp), and SR-B1 (50 bp), as well as the housekeeping gene GAPDH (94 bp). cDNA was run on an AbiPRISM 7300 fast real-time cycler using the power SYBR Green real-time PCR master mix kit and quantified by built-in SYBR Green Analysis.

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: .. Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. PCR amplification (in a 20 μ L scale) was performed with a heat‐resistant DNA polymerase (PrimeSTAR GXL, Takara) in a thermal cycler (Biometra, Gottingen, Germany) according to the following protocol;denaturation at 94°C for 2 min followed by 17–35 cycles of: denaturation (94°C, 10s), annealing (60°C, 10s), and extension (72°C, 30s).

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: The mRNA expression of α 1-(I)-procollagen, CD31, TIMP-1, MMP-9 and VEGF was evaluated by real-time PCR. .. Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction.

    Multiplex Assay:

    Article Title: Comparison of RNA extraction methods to augment the sensitivity for the differentiation of vesicular stomatitis virus Indiana1 and New Jersey
    Article Snippet: The use of this extraction method in addition to the multiplex RT‐PCR assay, allows a rapid approach for the differential detection of VSV‐IND and VSV‐NJ a single assay. .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples.

    RNA Expression:

    Article Title: Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
    Article Snippet: The effect of siRNA on the RNA expression of RAGE was assessed by real-time quantitative PCR after 48 hr of transfection. .. Total cellular RNA was extracted from HCAECs using an RNA isolation kit (RNeasy Mini kit, Qiagen, Duesseldorf, Germany).

    Agarose Gel Electrophoresis:

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562
    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA. .. The PCR amplicons were electrophoresed in a 1.2–1.5% agarose gel for 45–60 min, hybridized with ethidium bromide (Nacalai Tesque, Kyoto, Japan) or GelRed™ (Biotium, Fremont, CA), and visualized by ultraviolet or Blue/Green LED light, respectively.

    Preserving:

    Article Title: Quality Control of RNA Preservation and Extraction from Paraffin-Embedded Tissue: Implications for RT-PCR and Microarray Analysis
    Article Snippet: RNA Purification and Complementary DNA (cDNA) Synthesis Where indicated, the total RNA obtained by Trizol extraction was purified by processing with RNeasy Mini Kit (Qiagen) or RNeasy miRNA Mini Kit (Qiagen) according to manufactureŕs instructions. .. The Agilent 2100 Bioanalyzer (Agilent Technologies) was used to profile and compare RNA fragment length from the three different preservation methods with the RNA 6000 LabChip kit (Agilent Technologies).

    Spectrophotometry:

    Article Title: Quality Control of RNA Preservation and Extraction from Paraffin-Embedded Tissue: Implications for RT-PCR and Microarray Analysis
    Article Snippet: RNA Purification and Complementary DNA (cDNA) Synthesis Where indicated, the total RNA obtained by Trizol extraction was purified by processing with RNeasy Mini Kit (Qiagen) or RNeasy miRNA Mini Kit (Qiagen) according to manufactureŕs instructions. .. After extraction and purification, the RNA yield and purity was determined by measuring absorbance at 260 nm/280 nm on a Nanodrop spectrophotometer (Thermo Fisher Scientific).

    Produced:

    Article Title: Comparison of RNA Isolation Methods From Insect Larvae
    Article Snippet: Four methods including three commercial kits RNeasy Mini Kit (Qiagen), SV Total RNA isolation system (Promega), TRIzol reagent (Invitrogen), and a cetyl trimethylammonium bromide (CTAB)-based method were compared regarding their ability to isolate RNA from whole-body larvae ofThaumatotibia leucotreta (Meyrick),Thanatophilus micans (F.),Plutella xylostella (L.), andTenebrio molitor (L.). .. The CTAB-based method produced low-quality RNA and the Trizol reagent produced partially degraded RNA, whereas the RNeasy Mini Kit and SV Total RNA isolation system produced RNA of consistently high quality.

    Lysis:

    Article Title: Bias in recent miRBase annotations potentially associated with RNA quality issues
    Article Snippet: In detail, frozen tissue was homogenized in 700 μl QIAzol Lysis Reagent with using Tissue Lyser (Qiagen, Hilden, Germany) and incubated at room temperature for 5 min. After addition of 200 µl chloroform, samples were centrifuged at 12,000 rpm at 4 °C for phase separation. .. For determination of non-miRNA mediated background due to potential contamination with fragments of other RNAs during RNA degradation, total RNA was isolated from samples of three animals using RNeasy mini Kit (Qiagen, Hilden, Germany) with on column DNase digestion.

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