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Differential cytotoxic effects of ZnO NPs on human immune cell subsets. a <t>PBMC</t> were treated with varying concentrations (0, 0.5, 1, 2.5, 5, and 10 mM) of 8-nm ZnO NPs for 24 h and viability of CD3 + T cells, <t>CD4</t> + T cells, B cells, NK cells, and monocytes present in same PBMC cultures determined by monitoring PI uptake using flow cytometry. Data from three independent experiments are presented with error bars depicting standard error (SE). Asterisks denote statistically significant ( p
Pbmc, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 94/100, based on 948 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction"

Article Title: The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction

Journal: Nanoscale Research Letters

doi: 10.1007/s11671-009-9413-8

Differential cytotoxic effects of ZnO NPs on human immune cell subsets. a PBMC were treated with varying concentrations (0, 0.5, 1, 2.5, 5, and 10 mM) of 8-nm ZnO NPs for 24 h and viability of CD3 + T cells, CD4 + T cells, B cells, NK cells, and monocytes present in same PBMC cultures determined by monitoring PI uptake using flow cytometry. Data from three independent experiments are presented with error bars depicting standard error (SE). Asterisks denote statistically significant ( p
Figure Legend Snippet: Differential cytotoxic effects of ZnO NPs on human immune cell subsets. a PBMC were treated with varying concentrations (0, 0.5, 1, 2.5, 5, and 10 mM) of 8-nm ZnO NPs for 24 h and viability of CD3 + T cells, CD4 + T cells, B cells, NK cells, and monocytes present in same PBMC cultures determined by monitoring PI uptake using flow cytometry. Data from three independent experiments are presented with error bars depicting standard error (SE). Asterisks denote statistically significant ( p

Techniques Used: Flow Cytometry, Cytometry

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Selection:

Article Title: Incomplete Downregulation of CD4 Expression Affects HIV-1 Env Conformation and Antibody-Dependent Cellular Cytotoxicity Responses
Article Snippet: .. Briefly, PBMCs were obtained by leukapheresis, and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads (StemCell Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions and were activated with phytohemagglutinin-L (10 μg/ml) for 48 h and then maintained in RPMI 1640 complete medium supplemented with recombinant interleukin 2 (rIL-2) (100 U/ml). .. Proviral constructs.

Magnetic Beads:

Article Title: Adenovirally-Induced Polyfunctional T Cells Do Not Necessarily Recognize the Infected Target: Lessons from a Phase I Trial of the AERAS-402 Vaccine
Article Snippet: .. Second, CD8+ T cells were positively selected from PBMC using magnetic beads (Stemcell Technologies) such that > 97% of the cell population were CD8+ T cells. .. These CD8+ T cells were used as a source of responder T cells and tested in duplicate at a cell concentration of 250,000 cells per well.

Isolation:

Article Title: A small molecule inhibitor of p53 stimulates amplification of hematopoietic stem cells but does not promote tumor development in mice
Article Snippet: .. Immediately after TBI or later (1–5 days), BM-MNCs were isolated and plated (4 × 104 cells/ ml in 35 mm diameter plates) in MethoCult M3231 medium (StemCell Technologies, catalog #03231) supplemented with 10 ng/ml recombinant mouse GM-CSF (StemCell Technologies, catalog #02735) and IMDM (Invitrogen, catalog #12440–053). .. PFTβ (with or without IR) was either injected into mice or applied directly to the BM-MNCs before their plating.

Article Title: Plasma and Immunoglobulin G Galactosylation Associate with HIV Persistence During Antiretroviral Therapy
Article Snippet: .. CD4+ T cells were isolated from PBMCS using EasySep Human CD4+ T cell enrichment kit (Stemcell Technologies, Vancouver, British Columbia, Canada). .. Cellular RNA and DNA from total unfractionated PBMCs and isolated CD4+ T cells were purified using the AllPrep DNA/RNA kit (Qiagen, Ventura CA) as specified by the manufacturer, quantified using a Nanodrop (ND-1000) spectrophotometer and normalized to cell equivalents by qPCR using human genomic TERT for DNA and RPLP0 expression for RNA (Life Technologies, Grand Island NY).

Cell Culture:

Article Title: Transgene Insertion in Proximity to thec-myb Gene Disrupts Erythroid-Megakaryocytic Lineage Bifurcation ▿
Article Snippet: .. For the CFU-erythroid (CFU-E) assays, 1 × 105 BM-MNCs were cultured in methylcellulose medium (MethoCult M3231; StemCell Technologies) containing 1 U/ml recombinant human EPO (generous gift from Chugai Pharmaceutical). .. After 3 days of culturing, cells were stained with benzidine, and positive colonies were counted as CFU-E. For counting the burst-forming unit (BFU)-E-derived colonies, 2 × 104 MNCs were cultured with 2 U/ml EPO and 100 ng/ml of stem cell factor (SCF; R & D Systems) for 7 days.

Purification:

Article Title: Incomplete Downregulation of CD4 Expression Affects HIV-1 Env Conformation and Antibody-Dependent Cellular Cytotoxicity Responses
Article Snippet: .. Briefly, PBMCs were obtained by leukapheresis, and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads (StemCell Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions and were activated with phytohemagglutinin-L (10 μg/ml) for 48 h and then maintained in RPMI 1640 complete medium supplemented with recombinant interleukin 2 (rIL-2) (100 U/ml). .. Proviral constructs.

Recombinant:

Article Title: Transgene Insertion in Proximity to thec-myb Gene Disrupts Erythroid-Megakaryocytic Lineage Bifurcation ▿
Article Snippet: .. For the CFU-erythroid (CFU-E) assays, 1 × 105 BM-MNCs were cultured in methylcellulose medium (MethoCult M3231; StemCell Technologies) containing 1 U/ml recombinant human EPO (generous gift from Chugai Pharmaceutical). .. After 3 days of culturing, cells were stained with benzidine, and positive colonies were counted as CFU-E. For counting the burst-forming unit (BFU)-E-derived colonies, 2 × 104 MNCs were cultured with 2 U/ml EPO and 100 ng/ml of stem cell factor (SCF; R & D Systems) for 7 days.

Article Title: A small molecule inhibitor of p53 stimulates amplification of hematopoietic stem cells but does not promote tumor development in mice
Article Snippet: .. Immediately after TBI or later (1–5 days), BM-MNCs were isolated and plated (4 × 104 cells/ ml in 35 mm diameter plates) in MethoCult M3231 medium (StemCell Technologies, catalog #03231) supplemented with 10 ng/ml recombinant mouse GM-CSF (StemCell Technologies, catalog #02735) and IMDM (Invitrogen, catalog #12440–053). .. PFTβ (with or without IR) was either injected into mice or applied directly to the BM-MNCs before their plating.

Article Title: Incomplete Downregulation of CD4 Expression Affects HIV-1 Env Conformation and Antibody-Dependent Cellular Cytotoxicity Responses
Article Snippet: .. Briefly, PBMCs were obtained by leukapheresis, and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads (StemCell Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions and were activated with phytohemagglutinin-L (10 μg/ml) for 48 h and then maintained in RPMI 1640 complete medium supplemented with recombinant interleukin 2 (rIL-2) (100 U/ml). .. Proviral constructs.

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