verso cdna kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher verso cdna kit
    Verso Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/verso cdna kit/product/Thermo Fisher
    Average 99 stars, based on 306 article reviews
    Price from $9.99 to $1999.99
    verso cdna kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Countercurrent Chromatography:

    Article Title: Exosomes Released from M.tuberculosis Infected Cells Can Suppress IFN-? Mediated Activation of Na?ve Macrophages
    Article Snippet: 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific). .. The following primers were obtained commercially from Invitrogen: CIITA sense, 5′-ACG CTT TCT GGC TGG ATT AGT-3′ ; CIITA antisense,5′-TCA ACG CCA GTC TGA CGA AGG-3′ and GAPDH sense, 5′-AAC GAC CCC TTC ATT GAC-3′ , GAPDH antisense,5′- TCC ACG ACA TAC TCA GCAC-3′ .

    Real-time Polymerase Chain Reaction:

    Article Title: Dietary whey protein stimulates mitochondrial activity and decreases oxidative stress in mouse female brain
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis and quantitative real-time PCR (qRT-PCR) ... RNA samples (n=4 per treatment group) with 260/280 nm absorbance ratios ≥ 2.0 were used for qRT-PCR. cDNA synthesis was performed using an RNA-to-cDNA kit (Verso cDNA synthesis kit, #AB1453, Thermo Fisher Scientific, Waltham, MA).

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer. .. Quantitative PCR (qPCR) amplifications were conducted using SYBR green master mix (ABgene), as previously described ( ).

    Article Title: Exosomes Released from M.tuberculosis Infected Cells Can Suppress IFN-? Mediated Activation of Na?ve Macrophages
    Article Snippet: Paragraph title: Quantitative Real time PCR ... 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific).

    Article Title: Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
    Article Snippet: .. For confirmation of microarray results, RNA from normal- and crossbow-shaped cells was converted to cDNA using the Verso cDNA kit (Thermo-Scientific, Waltham, MA, USA) and quantitative PCR was performed using SYBR Green probes (Invitrogen) with an ABI7900HT real-time PCR instrument (Invitrogen). ..

    Article Title: Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana
    Article Snippet: Paragraph title: Real time PCR analysis of mdar transcript ... Total RNA (1000μl) was used to synthesis c-DNA by Verso cDNA synthesis Kit (Thermo Scientific India Pvt.

    In Vivo:

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: Bacterial RNA expressed in vivo was isolated from abscesses as previously described ( ). .. Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer.

    Amplification:

    Article Title: Dietary whey protein stimulates mitochondrial activity and decreases oxidative stress in mouse female brain
    Article Snippet: RNA samples (n=4 per treatment group) with 260/280 nm absorbance ratios ≥ 2.0 were used for qRT-PCR. cDNA synthesis was performed using an RNA-to-cDNA kit (Verso cDNA synthesis kit, #AB1453, Thermo Fisher Scientific, Waltham, MA). .. Thermocycling parameters were: 95°C for 3 min followed by 40 cycles of 95°C for 30 s, 55°C for 60 s, 72°C for 60 s. Primer sequences and amplicon size are given in .

    Article Title: Exosomes Released from M.tuberculosis Infected Cells Can Suppress IFN-? Mediated Activation of Na?ve Macrophages
    Article Snippet: 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific). .. PCR amplification efficiencies were determined for each gene prior to the relative quantification and were similar for the target gene (CIITA) and the endogenous control (GAPDH).

    Article Title: Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana
    Article Snippet: Total RNA (1000μl) was used to synthesis c-DNA by Verso cDNA synthesis Kit (Thermo Scientific India Pvt. .. Amplification conditions of Ecmdar under qPCR were as follows: initial denaturation at 95°C for 3min, followed by 40 cycles of denaturation at 95°C for 45 sec, 59°C for 30 sec, 72°C for 35 sec. qPCR was performed by ABI Step One real-time thermocycler.

    Agarose Gel Electrophoresis:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: One microgram of total RNA was used for cDNA production using oligo dT primer and the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol. .. PCR conditions were as follows: 95 °C, 3 min, 46 cycles of 95 °C, 45 s, 55 °C, 1 min, and 72 °C, 3 min, followed by 72 °C, 10 min. PCR products were resolved on 1.0% agarose gel stained with ethidium bromide.

    Synthesized:

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: .. Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer. .. Quantitative PCR (qPCR) amplifications were conducted using SYBR green master mix (ABgene), as previously described ( ).

    Article Title: Identification and characterization of proteins in theAmblyomma americanum tick cement cone
    Article Snippet: .. Template cDNA was synthesized from 200 or 500 ng of total RNA using a Verso cDNA synthesis kit (Thermo Fisher Scientific). .. The Verso cDNA synthesis kit contains a reverse transcriptase enhancer reagent, which prevents genomic DNA carryover into the synthesized cDNA.

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment
    Article Snippet: .. Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The synthesized cDNA was used as a template for qRT-PCR analysis, to estimate the expression level of the selected genes.

    Article Title: Epigenetic aspects of floral homeotic genes in relation to sexual dimorphism in the dioecious plant Mercurialis annua
    Article Snippet: .. The first strand of cDNA was synthesized from 1 µg total RNA treated with DNase (Epicentre) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific). .. Isolation of genes and partial promoter sequences Floral homeotic cDNA clones were prepared by PCR using M. annua flower cDNA as the template and appropriate degenerate primers (based on conserved regions of A. thaliana , R. communis , and J. curcas ; ) for the recovery of class B (AP3 , PI , TM6 ), class C/D (AG , AGL5 , AGL11 ), and two SUPERMAN -like (SUP -like) gene products.

    Isolation:

    Article Title: Dietary whey protein stimulates mitochondrial activity and decreases oxidative stress in mouse female brain
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis and quantitative real-time PCR (qRT-PCR) ... RNA samples (n=4 per treatment group) with 260/280 nm absorbance ratios ≥ 2.0 were used for qRT-PCR. cDNA synthesis was performed using an RNA-to-cDNA kit (Verso cDNA synthesis kit, #AB1453, Thermo Fisher Scientific, Waltham, MA).

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: Bacterial RNA expressed in vivo was isolated from abscesses as previously described ( ). .. Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer.

    Article Title: Exosomes Released from M.tuberculosis Infected Cells Can Suppress IFN-? Mediated Activation of Na?ve Macrophages
    Article Snippet: Quantitative Real time PCR RNA was isolated using RNeasy columns (Qiagen Inc. Valencia, CA) following manufacturer's instructions and quantified by Nanodropspectrophotometric method. .. 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific).

    Article Title: Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana
    Article Snippet: Real time PCR analysis of mdar transcript Total RNA was isolated from seedlings by RNA express reagent (Hi Media (P) Ltd.) as per manufacturer’s protocol. .. Total RNA (1000μl) was used to synthesis c-DNA by Verso cDNA synthesis Kit (Thermo Scientific India Pvt.

    Article Title: Microscale Gene Expression Analysis of Tumor-Associated Macrophages
    Article Snippet: .. ARCTURUS PicoPure RNA Isolation Kit (Catalog No.-KIT0204), Verso cDNA Synthesis Kit (Catalog No.-AB-1453/B), SuperScript VILO MasterMix (Catalog No.-11755050) and SuperScript IV First Strand Synthesis System (Catalog No.-18091050) were purchased from ThermoFisher Scientific (USA). .. The SYBR Green MasterMixes-FastStart Universal SYBR Green Master (Rox) (Catalog No.-04913914001), PowerUP SYBR Green MasterMix (Catalog No.-A25742) and Sso Advanced Universal IT SYBR Green SuperMix (Catalog No.-1725270) were purchased from Roche (Lifescience), Applied Biosystems (ThermoFisher Scientific) and Bio-Rad respectively.

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: Paragraph title: DNA and RNA isolation, cDNA production and expression analysis ... One microgram of total RNA was used for cDNA production using the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol.

    Size-exclusion Chromatography:

    Article Title: Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana
    Article Snippet: Total RNA (1000μl) was used to synthesis c-DNA by Verso cDNA synthesis Kit (Thermo Scientific India Pvt. .. Amplification conditions of Ecmdar under qPCR were as follows: initial denaturation at 95°C for 3min, followed by 40 cycles of denaturation at 95°C for 45 sec, 59°C for 30 sec, 72°C for 35 sec. qPCR was performed by ABI Step One real-time thermocycler.

    Quantitative RT-PCR:

    Article Title: Dietary whey protein stimulates mitochondrial activity and decreases oxidative stress in mouse female brain
    Article Snippet: .. RNA samples (n=4 per treatment group) with 260/280 nm absorbance ratios ≥ 2.0 were used for qRT-PCR. cDNA synthesis was performed using an RNA-to-cDNA kit (Verso cDNA synthesis kit, #AB1453, Thermo Fisher Scientific, Waltham, MA). .. Synthesis of cDNA was carried out using 0.5 μg total RNA. qRT-PCR was performed on an MJ Opticon (Bio-Rad, Hercules, CA) using SYBR Green 2X RT-PCR master mix (Bio-Rad) with a total reaction volume of 20 μL.

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: Paragraph title: Preparation of bacterial RNA and real-time RT-PCR. ... Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer.

    Article Title: Acetylcholinesterase (AChE) is an important link in the apoptotic pathway induced by hyperglycemia in Y79 retinoblastoma cell line
    Article Snippet: Paragraph title: RNA extraction and real-time RT-PCR ... RNA samples (0.2 μg) were used for cDNA synthesis prepared by the Verso™ cDNA Kit (Thermo SCIENTIFIC, UK).

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment
    Article Snippet: .. Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The synthesized cDNA was used as a template for qRT-PCR analysis, to estimate the expression level of the selected genes.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Dietary whey protein stimulates mitochondrial activity and decreases oxidative stress in mouse female brain
    Article Snippet: RNA samples (n=4 per treatment group) with 260/280 nm absorbance ratios ≥ 2.0 were used for qRT-PCR. cDNA synthesis was performed using an RNA-to-cDNA kit (Verso cDNA synthesis kit, #AB1453, Thermo Fisher Scientific, Waltham, MA). .. Synthesis of cDNA was carried out using 0.5 μg total RNA. qRT-PCR was performed on an MJ Opticon (Bio-Rad, Hercules, CA) using SYBR Green 2X RT-PCR master mix (Bio-Rad) with a total reaction volume of 20 μL.

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: .. Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer. .. Quantitative PCR (qPCR) amplifications were conducted using SYBR green master mix (ABgene), as previously described ( ).

    SYBR Green Assay:

    Article Title: Dietary whey protein stimulates mitochondrial activity and decreases oxidative stress in mouse female brain
    Article Snippet: RNA samples (n=4 per treatment group) with 260/280 nm absorbance ratios ≥ 2.0 were used for qRT-PCR. cDNA synthesis was performed using an RNA-to-cDNA kit (Verso cDNA synthesis kit, #AB1453, Thermo Fisher Scientific, Waltham, MA). .. Synthesis of cDNA was carried out using 0.5 μg total RNA. qRT-PCR was performed on an MJ Opticon (Bio-Rad, Hercules, CA) using SYBR Green 2X RT-PCR master mix (Bio-Rad) with a total reaction volume of 20 μL.

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer. .. Quantitative PCR (qPCR) amplifications were conducted using SYBR green master mix (ABgene), as previously described ( ).

    Article Title: Exosomes Released from M.tuberculosis Infected Cells Can Suppress IFN-? Mediated Activation of Na?ve Macrophages
    Article Snippet: 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific). .. One tenth of the resulting cDNA template was used for Real-time PCR using Absolute QPCR SYBR Green Mix (Thermo Scientific) and 7500 Fast Real Time PCR system (Applied Biosystems).

    Article Title: Acetylcholinesterase (AChE) is an important link in the apoptotic pathway induced by hyperglycemia in Y79 retinoblastoma cell line
    Article Snippet: RNA samples (0.2 μg) were used for cDNA synthesis prepared by the Verso™ cDNA Kit (Thermo SCIENTIFIC, UK). .. Real-time RT-PCR was performed using the Rotor-Gene Q (Qiagen, Corbett life science, Germany) and SYBR green master mix ABsolute™ Blue (Thermo SCIENTIFIC, UK).

    Article Title: Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
    Article Snippet: .. For confirmation of microarray results, RNA from normal- and crossbow-shaped cells was converted to cDNA using the Verso cDNA kit (Thermo-Scientific, Waltham, MA, USA) and quantitative PCR was performed using SYBR Green probes (Invitrogen) with an ABI7900HT real-time PCR instrument (Invitrogen). ..

    Article Title: Microscale Gene Expression Analysis of Tumor-Associated Macrophages
    Article Snippet: ARCTURUS PicoPure RNA Isolation Kit (Catalog No.-KIT0204), Verso cDNA Synthesis Kit (Catalog No.-AB-1453/B), SuperScript VILO MasterMix (Catalog No.-11755050) and SuperScript IV First Strand Synthesis System (Catalog No.-18091050) were purchased from ThermoFisher Scientific (USA). .. The SYBR Green MasterMixes-FastStart Universal SYBR Green Master (Rox) (Catalog No.-04913914001), PowerUP SYBR Green MasterMix (Catalog No.-A25742) and Sso Advanced Universal IT SYBR Green SuperMix (Catalog No.-1725270) were purchased from Roche (Lifescience), Applied Biosystems (ThermoFisher Scientific) and Bio-Rad respectively.

    Concentration Assay:

    Article Title: Acetylcholinesterase (AChE) is an important link in the apoptotic pathway induced by hyperglycemia in Y79 retinoblastoma cell line
    Article Snippet: RNA samples (0.2 μg) were used for cDNA synthesis prepared by the Verso™ cDNA Kit (Thermo SCIENTIFIC, UK). .. Serial dilutions of samples served to evaluate primers efficiency and the appropriate cDNA concentration that yields linear changes.

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment
    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The cDNA samples were diluted 1:10 (v/v) to the final template concentration for qRT-PCR.

    Nested PCR:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: One microgram of total RNA was used for cDNA production using oligo dT primer and the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol. .. Thereafter, the nested PCR was performed using CMT1-F and CMT1-R2 primers (Additional file : Table S1) using 5 µl of the first PCR product as template with same first PCR thermal cycler program and resolved as described above.

    Expressing:

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer. .. Gene expression was estimated by the ΔΔCT method relative to the expression of the endogenous reference gene gmk ( ).

    Article Title: Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
    Article Snippet: Paragraph title: Gene expression analysis ... For confirmation of microarray results, RNA from normal- and crossbow-shaped cells was converted to cDNA using the Verso cDNA kit (Thermo-Scientific, Waltham, MA, USA) and quantitative PCR was performed using SYBR Green probes (Invitrogen) with an ABI7900HT real-time PCR instrument (Invitrogen).

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment
    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The synthesized cDNA was used as a template for qRT-PCR analysis, to estimate the expression level of the selected genes.

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: Paragraph title: DNA and RNA isolation, cDNA production and expression analysis ... One microgram of total RNA was used for cDNA production using the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Staphylococcus aureus NorD, a Putative Efflux Pump Coregulated with the Opp1 Oligopeptide Permease, Contributes Selectively to Fitness In Vivo
    Article Snippet: All RNA samples were digested with DNase I (Turbo DNA- free ; Ambion, Austin, TX) to ensure the absence of detectable DNA by PCR. .. Real-time reverse transcription-PCR (RT-PCR) was performed in two steps. cDNA was synthesized using the Verso cDNA kit (ABgene, Epsom, Surrey, United Kingdom) with a gene-specific reverse primer.

    Article Title: Exosomes Released from M.tuberculosis Infected Cells Can Suppress IFN-? Mediated Activation of Na?ve Macrophages
    Article Snippet: 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific). .. PCR amplification efficiencies were determined for each gene prior to the relative quantification and were similar for the target gene (CIITA) and the endogenous control (GAPDH).

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: One microgram of total RNA was used for cDNA production using oligo dT primer and the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol. .. One-fifth of the resulting cDNA was subjected to first PCR (46 cycles) using Go-Taq polymerase (Promega) to amplify the CMT1 full-length cDNA with CMT1-F and CMT1-R1 primers (Additional file : Table S1).

    Article Title: Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana
    Article Snippet: Total RNA (1000μl) was used to synthesis c-DNA by Verso cDNA synthesis Kit (Thermo Scientific India Pvt. .. To perform qPCR, 12 μl of PCR reaction mixture was prepared, containing 2X SYBR® Premix Ex TaqTM buffer (Takara Clontech, TAKARA BIO INC Shiga Japan), 0.3μl of primers (0.25pmol/μl of each primer), 1μl of cDNA and molecular grade water.

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: One microgram of total RNA was used for cDNA production using the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol. .. The resulting cDNA was subjected to PCR to amplify the Evelknievel using primer set EK-RTF/EK-RTR and AtCOPIA18A using primers 18A-F/18A-R and Solo LTR using primer set Solo-F/Solo-R and AtMu1 using primer set AtMu1-F/AtMu1-R. Upstream analysis of CMT1 expression was performed using two primer sets, ex10-F(P1)/ex11-R(P2) and ex11F(P3)/ex13R(P4), and for downstream CMT1 expression, we used ex14-F(P5)/ex16-R(P6).

    Staining:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: One microgram of total RNA was used for cDNA production using oligo dT primer and the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol. .. PCR conditions were as follows: 95 °C, 3 min, 46 cycles of 95 °C, 45 s, 55 °C, 1 min, and 72 °C, 3 min, followed by 72 °C, 10 min. PCR products were resolved on 1.0% agarose gel stained with ethidium bromide.

    Article Title: Microscale Gene Expression Analysis of Tumor-Associated Macrophages
    Article Snippet: The Live/Dead Aqua staining kit was purchased from Invitrogen. .. ARCTURUS PicoPure RNA Isolation Kit (Catalog No.-KIT0204), Verso cDNA Synthesis Kit (Catalog No.-AB-1453/B), SuperScript VILO MasterMix (Catalog No.-11755050) and SuperScript IV First Strand Synthesis System (Catalog No.-18091050) were purchased from ThermoFisher Scientific (USA).

    Activated Clotting Time Assay:

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA
    Article Snippet: One microgram of total RNA was used for cDNA production using the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol. .. Actin or ubiquitin-10 was used as control (primer set ACT-F/ACT-R and UBQ10-F/UBQ10-R, respectively) (for primer sequences, see Additional file : Table S1).

    Microarray:

    Article Title: Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
    Article Snippet: .. For confirmation of microarray results, RNA from normal- and crossbow-shaped cells was converted to cDNA using the Verso cDNA kit (Thermo-Scientific, Waltham, MA, USA) and quantitative PCR was performed using SYBR Green probes (Invitrogen) with an ABI7900HT real-time PCR instrument (Invitrogen). ..

    RNA Extraction:

    Article Title: Acetylcholinesterase (AChE) is an important link in the apoptotic pathway induced by hyperglycemia in Y79 retinoblastoma cell line
    Article Snippet: Paragraph title: RNA extraction and real-time RT-PCR ... RNA samples (0.2 μg) were used for cDNA synthesis prepared by the Verso™ cDNA Kit (Thermo SCIENTIFIC, UK).

    Article Title: Identification and characterization of proteins in theAmblyomma americanum tick cement cone
    Article Snippet: Paragraph title: 2.2. Tick feeding, dissections and total RNA extraction ... Template cDNA was synthesized from 200 or 500 ng of total RNA using a Verso cDNA synthesis kit (Thermo Fisher Scientific).

    Software:

    Article Title: Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
    Article Snippet: Omics pathway analysis was performed with metacore software (GeneGo, San Diego, CA, USA). .. For confirmation of microarray results, RNA from normal- and crossbow-shaped cells was converted to cDNA using the Verso cDNA kit (Thermo-Scientific, Waltham, MA, USA) and quantitative PCR was performed using SYBR Green probes (Invitrogen) with an ABI7900HT real-time PCR instrument (Invitrogen).

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  • 99
    Thermo Fisher verso cdna kit
    mRNA expression of N-extended AChE, AChE-S, and AChE-R isoforms following treatment of Y79 cells with glucose for 1 h. Y79 cells were pre-treated for 16–24 h in starvation medium (1% FBS and 1 mg/ml of glucose) and then with 3.5 mg/ml glucose for different time intervals. Total <t>RNA</t> was extracted and <t>cDNA</t> was prepared for real-time PCR procedure as described under Materials and Methods. Results are presented as fold of control cells cultured in starvation media. Values are means ± SEM, ( N = 4). * p
    Verso Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/verso cdna kit/product/Thermo Fisher
    Average 99 stars, based on 706 article reviews
    Price from $9.99 to $1999.99
    verso cdna kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    mRNA expression of N-extended AChE, AChE-S, and AChE-R isoforms following treatment of Y79 cells with glucose for 1 h. Y79 cells were pre-treated for 16–24 h in starvation medium (1% FBS and 1 mg/ml of glucose) and then with 3.5 mg/ml glucose for different time intervals. Total RNA was extracted and cDNA was prepared for real-time PCR procedure as described under Materials and Methods. Results are presented as fold of control cells cultured in starvation media. Values are means ± SEM, ( N = 4). * p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Acetylcholinesterase (AChE) is an important link in the apoptotic pathway induced by hyperglycemia in Y79 retinoblastoma cell line

    doi: 10.3389/fnmol.2012.00069

    Figure Lengend Snippet: mRNA expression of N-extended AChE, AChE-S, and AChE-R isoforms following treatment of Y79 cells with glucose for 1 h. Y79 cells were pre-treated for 16–24 h in starvation medium (1% FBS and 1 mg/ml of glucose) and then with 3.5 mg/ml glucose for different time intervals. Total RNA was extracted and cDNA was prepared for real-time PCR procedure as described under Materials and Methods. Results are presented as fold of control cells cultured in starvation media. Values are means ± SEM, ( N = 4). * p

    Article Snippet: RNA samples (0.2 μg) were used for cDNA synthesis prepared by the Verso™ cDNA Kit (Thermo SCIENTIFIC, UK).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

    Differential expressions of genes modulating the mechanism of resistance to A. alternata in naturally infected mango fruits. (A) Effect of HWB on alternaria black spot (ABS) symptom development on mango cvs. Palmer, Kent, Tommy Atkins, Keitt, Lily and Shelly. (B) ABS symptom development on naturally infected fruits cv. Keitt following HWB treatment. (C) qRT-PCR differential expression profiling of genes Syn121 , glutardoxin , IT1K2 and AOS of cv. Shelly. Fruit peel tissues were sampled at four different time points after HWB treatment. RNA was extracted and served as a template for cDNA followed by qRT-PCR analysis of the genes of interest. Proportional increases in relative expression values were normalized against the samples of untreated mango fruits at 0 h. Expression data are means of two replicates. ABS-covered area was evaluated after 4 weeks of storage at 12°C. Average values followed by different letters differ significantly at P

    Journal: BMC Genomics

    Article Title: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatment

    doi: 10.1186/1471-2164-15-957

    Figure Lengend Snippet: Differential expressions of genes modulating the mechanism of resistance to A. alternata in naturally infected mango fruits. (A) Effect of HWB on alternaria black spot (ABS) symptom development on mango cvs. Palmer, Kent, Tommy Atkins, Keitt, Lily and Shelly. (B) ABS symptom development on naturally infected fruits cv. Keitt following HWB treatment. (C) qRT-PCR differential expression profiling of genes Syn121 , glutardoxin , IT1K2 and AOS of cv. Shelly. Fruit peel tissues were sampled at four different time points after HWB treatment. RNA was extracted and served as a template for cDNA followed by qRT-PCR analysis of the genes of interest. Proportional increases in relative expression values were normalized against the samples of untreated mango fruits at 0 h. Expression data are means of two replicates. ABS-covered area was evaluated after 4 weeks of storage at 12°C. Average values followed by different letters differ significantly at P

    Article Snippet: Analysis by qRT-PCR Single-stranded cDNA was synthesized from 1 μg of total RNA by means of the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Infection, Quantitative RT-PCR, Expressing

    EK transcriptional activation affects CMT1 downstream expression. a Schematic representation of the CMT1 gene and the insertion of EK within exon 13. CMT1 exons are numbered and shown as blue boxes and introns as black lines. Primers used to amplify upstream and downstream CMT1 sequences from cDNA are marked by arrowheads and numbered (P1–P7). The red broken line indicates the splice acceptor site of exon 14 (based on 27). EK LTRs are marked by red arrowheads. b CMT1 upstream transcript is alternatively spliced showing intron 12 retention. cDNA was prepared from RNA extracted from flowers derived from WT Col, WT Ler, ddm1, kyp2 , cmt3, ago4 and rdr2 and subjected to PCR to amplify CMT1 RNA sequences using primer set 1 + 2 (ex10-F + ex11-R) or primer set 3 + 4 (ex11-F + ex13-R). Note that primer set 3 + 4 yielded two PCR fragments SP1 and SP2. Actin was used as a reference. PCRs were performed for 35 cycles except for actin (30 cycles). M indicates DNA molecular size markers. The predicted alternative spliced variants SP1 and SP2 are schematically shown below. c CMT1 downstream expression using primer set 5 + 6 (ex14-F + ex16-R). WT Ler gDNA was used as a reference for PCR product derived from RNA. Note the enhanced downstream CMT1 expression in kyp2 and cmt3 mutants. Actin was used as a reference. Ler genomic DNA (gDNA) was used to confirm amplification from RNA. PCRs were for 35 cycles except for actin (30 cycles). d Analysis of splicing out of the entire EK retroelement using primer set 6 + 7 (ex13-F + ex16-R) on both sides of the EK insertion site. Note lack of a PCR product in kyp2 and cmt3 mutant. Actin was used as a reference RNA. Col genomic DNA (gDNA) was used to confirm amplification from RNA. PCR was for 42 cycles except for actin (30 cycles)

    Journal: Epigenetics & Chromatin

    Article Title: CMT3 and SUVH4/KYP silence the exonic Evelknievel retroelement to allow for reconstitution of CMT1 mRNA

    doi: 10.1186/s13072-018-0240-y

    Figure Lengend Snippet: EK transcriptional activation affects CMT1 downstream expression. a Schematic representation of the CMT1 gene and the insertion of EK within exon 13. CMT1 exons are numbered and shown as blue boxes and introns as black lines. Primers used to amplify upstream and downstream CMT1 sequences from cDNA are marked by arrowheads and numbered (P1–P7). The red broken line indicates the splice acceptor site of exon 14 (based on 27). EK LTRs are marked by red arrowheads. b CMT1 upstream transcript is alternatively spliced showing intron 12 retention. cDNA was prepared from RNA extracted from flowers derived from WT Col, WT Ler, ddm1, kyp2 , cmt3, ago4 and rdr2 and subjected to PCR to amplify CMT1 RNA sequences using primer set 1 + 2 (ex10-F + ex11-R) or primer set 3 + 4 (ex11-F + ex13-R). Note that primer set 3 + 4 yielded two PCR fragments SP1 and SP2. Actin was used as a reference. PCRs were performed for 35 cycles except for actin (30 cycles). M indicates DNA molecular size markers. The predicted alternative spliced variants SP1 and SP2 are schematically shown below. c CMT1 downstream expression using primer set 5 + 6 (ex14-F + ex16-R). WT Ler gDNA was used as a reference for PCR product derived from RNA. Note the enhanced downstream CMT1 expression in kyp2 and cmt3 mutants. Actin was used as a reference. Ler genomic DNA (gDNA) was used to confirm amplification from RNA. PCRs were for 35 cycles except for actin (30 cycles). d Analysis of splicing out of the entire EK retroelement using primer set 6 + 7 (ex13-F + ex16-R) on both sides of the EK insertion site. Note lack of a PCR product in kyp2 and cmt3 mutant. Actin was used as a reference RNA. Col genomic DNA (gDNA) was used to confirm amplification from RNA. PCR was for 42 cycles except for actin (30 cycles)

    Article Snippet: One microgram of total RNA was used for cDNA production using the Verso cDNA Kit (Thermo Scientific) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Expressing, Derivative Assay, Polymerase Chain Reaction, Amplification, Mutagenesis