veriti thermal cycler  (Thermo Fisher)


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    Structured Review

    Thermo Fisher veriti thermal cycler
    Veriti Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/veriti thermal cycler/product/Thermo Fisher
    Average 99 stars, based on 726 article reviews
    Price from $9.99 to $1999.99
    veriti thermal cycler - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
    Article Snippet: ISSR amplification was carried out in a 25 µL reaction system consisting of 2 µL of 10× reaction buffer (16 mM (NH)2 SO4 , 67 mM Tris–HCl with 25 mM MgCl2 ), 20 pM of ISSR primer, 200 µM dNTP mix, 25 ng of template DNA, and 1 unit of Taq DNA polymerase. .. The PCR reaction was executed in a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with the following conditions: 94 °C for 5 min tagged on by 40 cycles at 94 °C for 1 min, 45–58 °C (for different primers) for 1 min, 72 °C for 2 min followed by a final extension at 72 °C for 10 min.

    Article Title: A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost‐Effective SNP Typing
    Article Snippet: Each PCR was carried out in a total volume of 10 μl reaction mixture, with 30 ng of genomic DNA, 3 pmol of each primer, 1 μl 10× buffer, 1.5 mM MgCl2 , 200 μM dNTPs, and 0.5 U Taq polymerase (New England Biolabs, Ipswich, MA), using Veriti thermal cycler (Applied Biosystems, Foster City, CA). .. PCR conditions were as follows: initial denaturation of 95°C for 3 min, followed by 35 cycles of 94°C for 1 min, 61–65°C for 45 sec (gradient step), and 72°C for 45 sec, followed by a final extension of 72°C for 2 min. Amplified PCR products were separated on ethidium bromide (EtBr) stained 2% (wt/vol) agarose gels and 217 base pair products were visualized under UV light.

    Article Title: Intra-host symbiont diversity in eastern Pacific cold seep tubeworms identified by the 16S-V6 region, but undetected by the 16S-V4 region
    Article Snippet: .. A ~700 bp fragment of the COI gene was amplified on a Veriti thermal cycler (Applied Biosystems, CA, USA) using 10 p mol of primers jgLCO1490 and jgHCO2198 [ ], 12.5 μl AmpliTaq Gold Fast Master Mix (Thermo Fisher Scientific, Foster City, CA, USA) and > 20 n g of DNA adjusted to a total volume of 25 μl with PCR grade water. .. Negative controls without template were included on each PCR plate to check for sample contamination.

    Article Title: Identification of previously untypable RD cell line isolates and detection of EV-A71 genotype C1 in a child with AFP in Nigeria
    Article Snippet: Three second round PCR assays, each amplifying an ~350bp amplicon within the 5ʹ part of VP1 were done as described in Adeniji et al. [ ]. .. Thermal cycling was done using a Veriti Thermal cycler (Applied Biosystems, California, USA).

    Article Title: MSH2 Loss in Primary Prostate Cancer
    Article Snippet: MSI analyses were carried out using multiplex PCR with fluorescently-labelled primers, included in the MSI Analysis System, Version 1.2 (Promega Corp. Madison, WI, USA), for amplification of five mononucleotide repeat markers (NR-21, BAT-26, BAT-25, NR-24, MONO-27) and two pentanucleotide repeat loci (Penta-C and Penta-D) to confirm identity between the tumor and benign tissue pair. .. The PCR was performed using a Veriti Thermal Cycler (ThermoFisher Scientific) using the following program: 95°C 11min, 96°C for 1min, 10 cycles of 94°C for 30s, 58°C for 30s, 70°C for 1min; 20 cycles of 90°C for 30s, 58°C for 30s, 70°C for 1min; and 60°C for 30min.

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: .. PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold. .. For electrophoresis, a 1.25% (w/v) agarose gel in 1x TAE buffer was prepared with samples separated at 70 V for 90 minutes.

    Blocking Assay:

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
    Article Snippet: The PCR reaction was executed in a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with the following conditions: 94 °C for 5 min tagged on by 40 cycles at 94 °C for 1 min, 45–58 °C (for different primers) for 1 min, 72 °C for 2 min followed by a final extension at 72 °C for 10 min. .. 100 ng of genomic DNA of each sample was digested with EcoRI / MseI restriction endonuclease solution and linked with the corresponding block adapters.

    Real-time Polymerase Chain Reaction:

    Article Title: MicroRNA profiles in serum samples from Acute-On-Chronic Liver Failure patients and miR-25-3p as a potential biomarker for survival prediction
    Article Snippet: The polyadenylation and cDNA were prepared from total RNA using miRNA RT-qPCR Master Mix Detection Kit (Agilent Technologies, California, USA) in a Veriti Thermal Cycler (Applied Biosystems, California, USA). .. The qPCR was done using a StepOnePlus Real-Time PCR System (Applied Biosystems, California, USA) to quantify the levels of cel-miR-238-3p; miR-223-3p; miR-20a-5p; miR-106b-5p; miR-25-3p; miR-126-3p; and miR-1273g-3p.

    Incubation:

    Article Title: A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost‐Effective SNP Typing
    Article Snippet: Each PCR was carried out in a total volume of 10 μl reaction mixture, with 30 ng of genomic DNA, 3 pmol of each primer, 1 μl 10× buffer, 1.5 mM MgCl2 , 200 μM dNTPs, and 0.5 U Taq polymerase (New England Biolabs, Ipswich, MA), using Veriti thermal cycler (Applied Biosystems, Foster City, CA). .. Overnight incubated PCR‐amplified DNA was visualized in 2% (wt/vol) agarose gels after EtBr staining .

    Formalin-fixed Paraffin-Embedded:

    Article Title: Overexpression of circulating MiR-30b-5p identifies advanced breast cancer
    Article Snippet: .. MicroRNAs’ cDNA synthesis The cDNA synthesis from FFPE tissues RNA was performed in a Veriti® Thermal Cycler (Applied Biosystems, Foster City, CA, USA) using miRCURY LNA™ Universal RT microRNA PCR (Exiqon, Vedbaek, Denmark) following manufacturer’s instructions. .. Circulating RNA was reverse transcribed to cDNA using TaqMan Advanced miRNA cDNA synthesis kit (Applied Biosystems, Foster City, CA, USA).

    Expressing:

    Article Title: MicroRNA profiles in serum samples from Acute-On-Chronic Liver Failure patients and miR-25-3p as a potential biomarker for survival prediction
    Article Snippet: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Expression of selected mature miRNAs was assessed by RT-qPCR. .. The polyadenylation and cDNA were prepared from total RNA using miRNA RT-qPCR Master Mix Detection Kit (Agilent Technologies, California, USA) in a Veriti Thermal Cycler (Applied Biosystems, California, USA).

    Generated:

    Article Title: Longitudinal monitoring of KRAS-mutated circulating tumor DNA enables the prediction of prognosis and therapeutic responses in patients with pancreatic cancer
    Article Snippet: .. The generated droplets (approximately 15000 generated droplets per well) were transferred to a 96-well reaction plate, heat-sealed with a foil lid, and subjected to thermocycling in a Veriti thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA) under the following cycling conditions: 95°C for 10 min and 40 cycles at 95°C for 30 s and subsequently at 55°C for 90 s. Amplified droplets were analyzed using a QX200 droplet reader (Bio-Rad Laboratories, Hercules, CA, USA) for the fluorescent measurement of FAM and HEX probes for wild-type and mutant genes, respectively. .. QuantaSoft software (Bio-Rad Laboratories, Hercules, CA, USA) was used to measure the number of positive and negative droplets, and their ratio (mutated allele frequency) was fitted to a Poisson distribution to determine the target copy number/mL in the input reaction.

    Polymerase Chain Reaction:

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
    Article Snippet: .. The PCR reaction was executed in a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with the following conditions: 94 °C for 5 min tagged on by 40 cycles at 94 °C for 1 min, 45–58 °C (for different primers) for 1 min, 72 °C for 2 min followed by a final extension at 72 °C for 10 min. .. The amplified products were resolved on 1.5% agarose gel in 1× TBE buffer at 60 volts for 3 h. The AFLP analysis was performed using the standard protocol (Vos et al. ) with desired modifications.

    Article Title: Overexpression of circulating MiR-30b-5p identifies advanced breast cancer
    Article Snippet: .. MicroRNAs’ cDNA synthesis The cDNA synthesis from FFPE tissues RNA was performed in a Veriti® Thermal Cycler (Applied Biosystems, Foster City, CA, USA) using miRCURY LNA™ Universal RT microRNA PCR (Exiqon, Vedbaek, Denmark) following manufacturer’s instructions. .. Circulating RNA was reverse transcribed to cDNA using TaqMan Advanced miRNA cDNA synthesis kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Antiretroviral therapy-induced paradoxical worsening of previously healed Mycobacterium haemophilum cutaneous lesions in advanced HIV infection
    Article Snippet: PCR master mix contained 5X KAPA2G Robust HotStart ReadyMix, 10 μM each of the primers, 5% DMSO (100%), PCR-grade water and 5 µL of target DNA for the 25 µL of final of reaction volume. .. Amplifications were performed in a Veriti Thermal Cycler (Applied Biosystems,Thermo Fisher Scientific, Waltham, Massachusetts, USA), performed using the following cycling conditions.

    Article Title: A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost‐Effective SNP Typing
    Article Snippet: .. Each PCR was carried out in a total volume of 10 μl reaction mixture, with 30 ng of genomic DNA, 3 pmol of each primer, 1 μl 10× buffer, 1.5 mM MgCl2 , 200 μM dNTPs, and 0.5 U Taq polymerase (New England Biolabs, Ipswich, MA), using Veriti thermal cycler (Applied Biosystems, Foster City, CA). .. PCR conditions were as follows: initial denaturation of 95°C for 3 min, followed by 35 cycles of 94°C for 1 min, 61–65°C for 45 sec (gradient step), and 72°C for 45 sec, followed by a final extension of 72°C for 2 min. Amplified PCR products were separated on ethidium bromide (EtBr) stained 2% (wt/vol) agarose gels and 217 base pair products were visualized under UV light.

    Article Title: Intra-host symbiont diversity in eastern Pacific cold seep tubeworms identified by the 16S-V6 region, but undetected by the 16S-V4 region
    Article Snippet: .. A ~700 bp fragment of the COI gene was amplified on a Veriti thermal cycler (Applied Biosystems, CA, USA) using 10 p mol of primers jgLCO1490 and jgHCO2198 [ ], 12.5 μl AmpliTaq Gold Fast Master Mix (Thermo Fisher Scientific, Foster City, CA, USA) and > 20 n g of DNA adjusted to a total volume of 25 μl with PCR grade water. .. Negative controls without template were included on each PCR plate to check for sample contamination.

    Article Title: Identification of previously untypable RD cell line isolates and detection of EV-A71 genotype C1 in a child with AFP in Nigeria
    Article Snippet: Paragraph title: Polymerase chain reaction (PCR) assays ... Thermal cycling was done using a Veriti Thermal cycler (Applied Biosystems, California, USA).

    Article Title: MSH2 Loss in Primary Prostate Cancer
    Article Snippet: .. The PCR was performed using a Veriti Thermal Cycler (ThermoFisher Scientific) using the following program: 95°C 11min, 96°C for 1min, 10 cycles of 94°C for 30s, 58°C for 30s, 70°C for 1min; 20 cycles of 90°C for 30s, 58°C for 30s, 70°C for 1min; and 60°C for 30min. .. PCR products were mixed with formamide and size standard, denatured and run on an ABI (Waltham, MA) 3130 capillary electrophoresis instrument using injection times of 30–180 seconds.

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: .. PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold. .. For electrophoresis, a 1.25% (w/v) agarose gel in 1x TAE buffer was prepared with samples separated at 70 V for 90 minutes.

    Article Title: MicroRNA profiles in serum samples from Acute-On-Chronic Liver Failure patients and miR-25-3p as a potential biomarker for survival prediction
    Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... The polyadenylation and cDNA were prepared from total RNA using miRNA RT-qPCR Master Mix Detection Kit (Agilent Technologies, California, USA) in a Veriti Thermal Cycler (Applied Biosystems, California, USA).

    Injection:

    Article Title: MSH2 Loss in Primary Prostate Cancer
    Article Snippet: The PCR was performed using a Veriti Thermal Cycler (ThermoFisher Scientific) using the following program: 95°C 11min, 96°C for 1min, 10 cycles of 94°C for 30s, 58°C for 30s, 70°C for 1min; 20 cycles of 90°C for 30s, 58°C for 30s, 70°C for 1min; and 60°C for 30min. .. PCR products were mixed with formamide and size standard, denatured and run on an ABI (Waltham, MA) 3130 capillary electrophoresis instrument using injection times of 30–180 seconds.

    Fluorescence:

    Article Title: MicroRNA profiles in serum samples from Acute-On-Chronic Liver Failure patients and miR-25-3p as a potential biomarker for survival prediction
    Article Snippet: The polyadenylation and cDNA were prepared from total RNA using miRNA RT-qPCR Master Mix Detection Kit (Agilent Technologies, California, USA) in a Veriti Thermal Cycler (Applied Biosystems, California, USA). .. The relative expression of the miRNAs was calculated by the comparative quantification cycle (Cq) method , where the Cq is the PCR cycle number at which the sample fluorescence signal passes a fixed threshold line, and reported as 2−ΔCq (miR-target – miR-1273g-3p) .

    Mutagenesis:

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: Four primers, when used simultaneously in PCR amplification, were expected to yield two amplicons in homozygous wildtype individuals and three amplicons in individuals carrying at least one copy of the mutant allele. .. PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold.

    Article Title: Longitudinal monitoring of KRAS-mutated circulating tumor DNA enables the prediction of prognosis and therapeutic responses in patients with pancreatic cancer
    Article Snippet: .. The generated droplets (approximately 15000 generated droplets per well) were transferred to a 96-well reaction plate, heat-sealed with a foil lid, and subjected to thermocycling in a Veriti thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA) under the following cycling conditions: 95°C for 10 min and 40 cycles at 95°C for 30 s and subsequently at 55°C for 90 s. Amplified droplets were analyzed using a QX200 droplet reader (Bio-Rad Laboratories, Hercules, CA, USA) for the fluorescent measurement of FAM and HEX probes for wild-type and mutant genes, respectively. .. QuantaSoft software (Bio-Rad Laboratories, Hercules, CA, USA) was used to measure the number of positive and negative droplets, and their ratio (mutated allele frequency) was fitted to a Poisson distribution to determine the target copy number/mL in the input reaction.

    Negative Control:

    Article Title: Antiretroviral therapy-induced paradoxical worsening of previously healed Mycobacterium haemophilum cutaneous lesions in advanced HIV infection
    Article Snippet: Amplifications were performed in a Veriti Thermal Cycler (Applied Biosystems,Thermo Fisher Scientific, Waltham, Massachusetts, USA), performed using the following cycling conditions. .. Negative control PCRs containing no template DNA, were also included to control DNA contamination.

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold. .. Each PCR amplicon in a known affected cell line and a random bred negative control was gel extracted and Sanger sequenced at the MU DNA Core on an ABI 3730XL (ABI, Foster City, CA) and assembled into reference-based contigs using Sequencher (GeneCodes, Ann Arbor, MI).

    Size-exclusion Chromatography:

    Article Title: A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost‐Effective SNP Typing
    Article Snippet: Each PCR was carried out in a total volume of 10 μl reaction mixture, with 30 ng of genomic DNA, 3 pmol of each primer, 1 μl 10× buffer, 1.5 mM MgCl2 , 200 μM dNTPs, and 0.5 U Taq polymerase (New England Biolabs, Ipswich, MA), using Veriti thermal cycler (Applied Biosystems, Foster City, CA). .. PCR conditions were as follows: initial denaturation of 95°C for 3 min, followed by 35 cycles of 94°C for 1 min, 61–65°C for 45 sec (gradient step), and 72°C for 45 sec, followed by a final extension of 72°C for 2 min. Amplified PCR products were separated on ethidium bromide (EtBr) stained 2% (wt/vol) agarose gels and 217 base pair products were visualized under UV light.

    Sequencing:

    Article Title: Intra-host symbiont diversity in eastern Pacific cold seep tubeworms identified by the 16S-V6 region, but undetected by the 16S-V4 region
    Article Snippet: A ~700 bp fragment of the COI gene was amplified on a Veriti thermal cycler (Applied Biosystems, CA, USA) using 10 p mol of primers jgLCO1490 and jgHCO2198 [ ], 12.5 μl AmpliTaq Gold Fast Master Mix (Thermo Fisher Scientific, Foster City, CA, USA) and > 20 n g of DNA adjusted to a total volume of 25 μl with PCR grade water. .. Sequence analysis was performed with GENEIOUS v9.1.8 ( http://www.geneious.com/ ) as described in [ ].

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: Paragraph title: PCR validation and Sanger sequencing ... PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold.

    Quantitative RT-PCR:

    Article Title: MicroRNA profiles in serum samples from Acute-On-Chronic Liver Failure patients and miR-25-3p as a potential biomarker for survival prediction
    Article Snippet: .. The polyadenylation and cDNA were prepared from total RNA using miRNA RT-qPCR Master Mix Detection Kit (Agilent Technologies, California, USA) in a Veriti Thermal Cycler (Applied Biosystems, California, USA). .. The qPCR was done using a StepOnePlus Real-Time PCR System (Applied Biosystems, California, USA) to quantify the levels of cel-miR-238-3p; miR-223-3p; miR-20a-5p; miR-106b-5p; miR-25-3p; miR-126-3p; and miR-1273g-3p.

    Polymorphism Assay:

    Article Title: A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost‐Effective SNP Typing
    Article Snippet: Paragraph title: PCR and Mutagenic Restriction Fragment Length Polymorphism Assay ... Each PCR was carried out in a total volume of 10 μl reaction mixture, with 30 ng of genomic DNA, 3 pmol of each primer, 1 μl 10× buffer, 1.5 mM MgCl2 , 200 μM dNTPs, and 0.5 U Taq polymerase (New England Biolabs, Ipswich, MA), using Veriti thermal cycler (Applied Biosystems, Foster City, CA).

    Software:

    Article Title: Longitudinal monitoring of KRAS-mutated circulating tumor DNA enables the prediction of prognosis and therapeutic responses in patients with pancreatic cancer
    Article Snippet: The generated droplets (approximately 15000 generated droplets per well) were transferred to a 96-well reaction plate, heat-sealed with a foil lid, and subjected to thermocycling in a Veriti thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA) under the following cycling conditions: 95°C for 10 min and 40 cycles at 95°C for 30 s and subsequently at 55°C for 90 s. Amplified droplets were analyzed using a QX200 droplet reader (Bio-Rad Laboratories, Hercules, CA, USA) for the fluorescent measurement of FAM and HEX probes for wild-type and mutant genes, respectively. .. QuantaSoft software (Bio-Rad Laboratories, Hercules, CA, USA) was used to measure the number of positive and negative droplets, and their ratio (mutated allele frequency) was fitted to a Poisson distribution to determine the target copy number/mL in the input reaction.

    Electrophoresis:

    Article Title: MSH2 Loss in Primary Prostate Cancer
    Article Snippet: The PCR was performed using a Veriti Thermal Cycler (ThermoFisher Scientific) using the following program: 95°C 11min, 96°C for 1min, 10 cycles of 94°C for 30s, 58°C for 30s, 70°C for 1min; 20 cycles of 90°C for 30s, 58°C for 30s, 70°C for 1min; and 60°C for 30min. .. PCR products were mixed with formamide and size standard, denatured and run on an ABI (Waltham, MA) 3130 capillary electrophoresis instrument using injection times of 30–180 seconds.

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold. .. For electrophoresis, a 1.25% (w/v) agarose gel in 1x TAE buffer was prepared with samples separated at 70 V for 90 minutes.

    Multiplex Assay:

    Article Title: MSH2 Loss in Primary Prostate Cancer
    Article Snippet: MSI analyses were carried out using multiplex PCR with fluorescently-labelled primers, included in the MSI Analysis System, Version 1.2 (Promega Corp. Madison, WI, USA), for amplification of five mononucleotide repeat markers (NR-21, BAT-26, BAT-25, NR-24, MONO-27) and two pentanucleotide repeat loci (Penta-C and Penta-D) to confirm identity between the tumor and benign tissue pair. .. The PCR was performed using a Veriti Thermal Cycler (ThermoFisher Scientific) using the following program: 95°C 11min, 96°C for 1min, 10 cycles of 94°C for 30s, 58°C for 30s, 70°C for 1min; 20 cycles of 90°C for 30s, 58°C for 30s, 70°C for 1min; and 60°C for 30min.

    Agarose Gel Electrophoresis:

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
    Article Snippet: The PCR reaction was executed in a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with the following conditions: 94 °C for 5 min tagged on by 40 cycles at 94 °C for 1 min, 45–58 °C (for different primers) for 1 min, 72 °C for 2 min followed by a final extension at 72 °C for 10 min. .. The amplified products were resolved on 1.5% agarose gel in 1× TBE buffer at 60 volts for 3 h. The AFLP analysis was performed using the standard protocol (Vos et al. ) with desired modifications.

    Article Title: Identification of previously untypable RD cell line isolates and detection of EV-A71 genotype C1 in a child with AFP in Nigeria
    Article Snippet: Thermal cycling was done using a Veriti Thermal cycler (Applied Biosystems, California, USA). .. PCR products of assay 1 and the second round PCR product of assay 2 were resolved on a 2% Agarose gel stained with ethidium bromide and viewed by ultraviolet (UV) light on a transilluminator.

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold. .. For electrophoresis, a 1.25% (w/v) agarose gel in 1x TAE buffer was prepared with samples separated at 70 V for 90 minutes.

    Produced:

    Article Title: Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST
    Article Snippet: PCR validation and Sanger sequencing Primers were designed using the reference sequence surrounding the 5′, internal, and 3′ breakpoints of the duplicated region (Supplementary Table ) to validate the segmental duplication in the cell lines and cats produced by AI. .. PCR products were amplified using an Applied Biosystems Veriti thermal cycler (Foster City, CA) with the following conditions: 94 °C for 3:00 min denaturation, followed by 35 cycles at 94 °C for 1:00 min, 58 °C for 1:00 min, and 72 °C for 1:00 min, which ended with 72 °C for 10:00 min and 4 °C hold.

    Marker:

    Article Title: Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)
    Article Snippet: Based on parental screening, 28 ISSRs and 16 AFLP primer combinations were used to amplify DNA from the two parents and resistant and susceptible bulks for a co-segregating polymorphic marker study. .. The PCR reaction was executed in a Veriti thermal cycler (Applied Biosystems, Foster City, CA, USA) with the following conditions: 94 °C for 5 min tagged on by 40 cycles at 94 °C for 1 min, 45–58 °C (for different primers) for 1 min, 72 °C for 2 min followed by a final extension at 72 °C for 10 min.

    Staining:

    Article Title: A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost‐Effective SNP Typing
    Article Snippet: Each PCR was carried out in a total volume of 10 μl reaction mixture, with 30 ng of genomic DNA, 3 pmol of each primer, 1 μl 10× buffer, 1.5 mM MgCl2 , 200 μM dNTPs, and 0.5 U Taq polymerase (New England Biolabs, Ipswich, MA), using Veriti thermal cycler (Applied Biosystems, Foster City, CA). .. PCR conditions were as follows: initial denaturation of 95°C for 3 min, followed by 35 cycles of 94°C for 1 min, 61–65°C for 45 sec (gradient step), and 72°C for 45 sec, followed by a final extension of 72°C for 2 min. Amplified PCR products were separated on ethidium bromide (EtBr) stained 2% (wt/vol) agarose gels and 217 base pair products were visualized under UV light.

    Article Title: Identification of previously untypable RD cell line isolates and detection of EV-A71 genotype C1 in a child with AFP in Nigeria
    Article Snippet: Thermal cycling was done using a Veriti Thermal cycler (Applied Biosystems, California, USA). .. PCR products of assay 1 and the second round PCR product of assay 2 were resolved on a 2% Agarose gel stained with ethidium bromide and viewed by ultraviolet (UV) light on a transilluminator.

    Variant Assay:

    Article Title: Longitudinal monitoring of KRAS-mutated circulating tumor DNA enables the prediction of prognosis and therapeutic responses in patients with pancreatic cancer
    Article Snippet: The reaction mixture comprised of 10 μL of 2× ddPCR Supermix, 1 μL of each reference and variant 20× Bio-Rad PrimePCR KRAS for ddPCR, and 10 μL of sample eluted from plasma in a final volume of 22 μL. .. The generated droplets (approximately 15000 generated droplets per well) were transferred to a 96-well reaction plate, heat-sealed with a foil lid, and subjected to thermocycling in a Veriti thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA) under the following cycling conditions: 95°C for 10 min and 40 cycles at 95°C for 30 s and subsequently at 55°C for 90 s. Amplified droplets were analyzed using a QX200 droplet reader (Bio-Rad Laboratories, Hercules, CA, USA) for the fluorescent measurement of FAM and HEX probes for wild-type and mutant genes, respectively.

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    Thermo Fisher veriti 96 well thermal cycler
    Veriti 96 Well Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/veriti 96 well thermal cycler/product/Thermo Fisher
    Average 90 stars, based on 376 article reviews
    Price from $9.99 to $1999.99
    veriti 96 well thermal cycler - by Bioz Stars, 2020-02
    90/100 stars
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    The Veriti Dx 384 Well Thermal Cycler delivers the proven reliability you expect from Applied Biosystems for in vitro diagnostic PCR use It features an easy to use color touch
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    The Temperature Verification Kits and Probes are engineered and manufactured specifically for Applied Biosystems to validate temperature calibration and uniformity in GeneAmp PCR and the Veriti Thermal Cycler instrument systems
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