vegf165  (R&D Systems)

Journal: The American Journal of Pathology

Article Title: Overexpression of Vascular Endothelial Growth Factor 189 in Breast Cancer Cells Leads to Delayed Tumor Uptake with Dilated Intratumoral Vessels

doi: 10.2353/ajpath.2008.070181

Figure Lengend Snippet: Endothelial cell proliferation by VEGF189 and VEGF165 produced by selected MDA-MB-231 cells. HUVECs (5 × 10 3 /well) were incubated with the conditioned medium of selected V165 and V189 clones, as described in Materials and Methods; the proliferation

Article Snippet: Recombinant human VEGF165 (R & D Systems) and VEGF189 were used as controls.

Techniques: Produced, Multiple Displacement Amplification, Incubation, Clone Assay

Journal: The American Journal of Pathology

Article Title: Overexpression of Vascular Endothelial Growth Factor 189 in Breast Cancer Cells Leads to Delayed Tumor Uptake with Dilated Intratumoral Vessels

doi: 10.2353/ajpath.2008.070181

Figure Lengend Snippet: Generation of MDA-MB-231 Cells Stably Expressing VEGF189 or VEGF165 Protein

Article Snippet: Recombinant human VEGF165 (R & D Systems) and VEGF189 were used as controls.

Techniques: Multiple Displacement Amplification, Stable Transfection, Expressing

Journal: The American Journal of Pathology

Article Title: Overexpression of Vascular Endothelial Growth Factor 189 in Breast Cancer Cells Leads to Delayed Tumor Uptake with Dilated Intratumoral Vessels

doi: 10.2353/ajpath.2008.070181

Figure Lengend Snippet: Growth curves of MDA-overexpressing VEGF189 and VEGF165 tumors induced in SCID mice. Cells (5 × 10 6 ) were subcutaneously injected in SCID mice as described in Materials and Methods. A: Tumors were generated from V189-13 or V165-42 clone, or cV

Article Snippet: Recombinant human VEGF165 (R & D Systems) and VEGF189 were used as controls.

Techniques: Multiple Displacement Amplification, Mouse Assay, Injection, Generated

Journal: Molecular Therapy. Nucleic Acids

Article Title: Binding and Structural Properties of DNA Aptamers with VEGF-A-Mimic Activity

doi: 10.1016/j.omtn.2019.12.034

Figure Lengend Snippet: In Vitro Tube Formation Assay Using Human Umbilical Vein Endothelial Cells (HUVECs) on a Three-Dimensional Gel Consisting of Diluted Matrigel The cells were treated with Apt01 (10 μM), Apt02 (10 μM), or VEGF165 (10 ng/mL, 0.26 nM) for 24 h. (A) Representative images of tube formation of HUVECs on Matrigel, which are treated by aptamers or VEGF165. Scale bars, 1 mm. (B) Time course of mesh number in the images of the HUVEC networks at 4, 7, and 24 h. Error bars represent standard deviation of the mean (n = 3 plots). (C–E) Boxplot showing mesh number in the images of the HUVEC networks at (C) 4, (D) 7, and (E) 24 h (n = 3 plots). *p

Article Snippet: HUVECs (1.1 × 105 cells/well) were placed into 24-well flat-bottomed plates pre-coated with Matrigel and then incubated with aptamers (10 μM) or VEGF165 (Recombinant Human VEGF 165 Protein, Cat. No. 293-VE [R & D Systems, USA]; 10 ng/mL, 0.26 nM) for 24 h. In the experiment of dose dependency, the Apt02 concentration of more than 10 μM showed the significant difference from control group (data not shown).

Techniques: In Vitro, Tube Formation Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro

doi: 10.3390/ijms12031744

Figure Lengend Snippet: Supernatants of MSCs in each group (Control group, BMP group, VEGF group and BMP+VEGF group) were collected at 1, 4, and 8 weeks after transfection and measured with ELISA kits specific for BMP2 (A) and VEGF165 (B) . Results are shown as mean ±SD (n = 4 for each group).

Article Snippet: Enzyme Linked Immunoabsorbent Assay (ELISA) The in vitro hBMP2 and hVEGF165 production in each group was quantified in culture supernatant at 1, 4, and 8 weeks after transduction using an ELISA kit (BMP2 ELISA kit, R & D system; VEGF165 ELISA kit, BIOSOURCE) according to the manufacturer’s instructions.

Techniques: Transfection, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: MicroRNA 139-5p coordinates APLNR-CXCR4 crosstalk during vascular maturation

doi: 10.1038/ncomms11268

Figure Lengend Snippet: APLNR regulates CXCR4 expression through miR-139-5p. ( a , b ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent APLNR knockdown. mRNA ( a ) and protein levels ( b ) are shown. ( c ) CXCR4 expression in response to AGO2 knockdown. ( d ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent AGO2 knockdown. ( e ) Determination of miR-139-5p levels in FACS sorted ECs from P5 retinas of Aplnr −/− , Apln −/− mice and respective littermates. n= 3 retinas per genotype. ( f ) CXCR4 expression in HUVECs overexpressing miR-139-5p mimic or non-targeting control anti-miR. ( g ) Expression levels of miR-139-5p in response to shear stress, with or without concurrent APLNR knockdown. ( h ) CXCR4 expression in HUVECs in response to shear stress exposure, with or without concurrent miR-139-5p inhibition via anti-miR. ( i ) Sprouting assay using HUVEC covered beads transduced with miR-139-5p or control lentivirus in response to SDF-1α or VEGF 165. Scale bar, 175 μm. ( j ) Migration assay of HUVECs transduced with miR-139-5p or control lentivirus in response to SDF-1α. Scale bar, 200 μm. * P

Article Snippet: The fibrinogen/bead solution was allowed to clot for 5 min at room temperature and then at 37 °C in 5% CO2 for 15 min. EGM-2 containing 50 ng ml−1 SDF-1α (SRP3252, Sigma-Aldrich), or 20 ng ml−1 VEGF 165 (293-VE, R & D Systems) was used.

Techniques: Expressing, FACS, Mouse Assay, Inhibition, Transduction, Migration