vegf165  (R&D Systems)

 
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    Name:
    Human VEGF165 Antibody
    Description:
    The Human VEGF165 Antibody from R D Systems is a goat polyclonal antibody to VEGF This antibody reacts with human The Human VEGF165 Antibody has been validated for the following applications Western Blot Neutralization Immunocytochemistry
    Catalog Number:
    AF-293-NA
    Price:
    375
    Category:
    Primary Antibodies
    Applications:
    Western Blot, Neutralization, Immunocytochemistry
    Purity:
    Antigen Affinity-purified
    Conjugate:
    Unconjugated
    Immunogen:
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165, Ala27-Arg191, Accession # NP_001165097.1
    Size:
    100 ug
    Host:
    Goat
    Isotype:
    IgG
    Buy from Supplier


    Structured Review

    R&D Systems vegf165
    Human VEGF165 Antibody
    The Human VEGF165 Antibody from R D Systems is a goat polyclonal antibody to VEGF This antibody reacts with human The Human VEGF165 Antibody has been validated for the following applications Western Blot Neutralization Immunocytochemistry
    https://www.bioz.com/result/vegf165/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf165 - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "TTAC-0001, a human monoclonal antibody targeting VEGFR-2/KDR, blocks tumor angiogenesis"

    Article Title: TTAC-0001, a human monoclonal antibody targeting VEGFR-2/KDR, blocks tumor angiogenesis

    Journal: mAbs

    doi: 10.1080/19420862.2015.1045168

    Characterization of binding properties of anti-KDR antibodies. Competitive inhibition of anti-KDR phages ( a ) or antibodies ( b ) in binding of KDR(ECD1–3)-Fc to VEGF165. TTAC-0001, closed circle; 6C1, open circle; 6G1, triangle. ( c ) Complementarity-determining region (CDR) sequences of anti-KDR antibodies and their respective KDR binding affinities (K d ) determined using surface plasmon resonance.
    Figure Legend Snippet: Characterization of binding properties of anti-KDR antibodies. Competitive inhibition of anti-KDR phages ( a ) or antibodies ( b ) in binding of KDR(ECD1–3)-Fc to VEGF165. TTAC-0001, closed circle; 6C1, open circle; 6G1, triangle. ( c ) Complementarity-determining region (CDR) sequences of anti-KDR antibodies and their respective KDR binding affinities (K d ) determined using surface plasmon resonance.

    Techniques Used: Binding Assay, Inhibition, SPR Assay

    Measurement of the specificity of TTAC-0001. ( a ) Binding of TTAC-0001 to VEGF isoforms. Black bar represents VEGF-165. Gray bar represents VEGF-C and blank bar represents VEGF-D. hIgG and bevacizumab were used as controls. ( b ) Specificity measurement of TTAC-0001 on VEGFRs by Biacore. Line, hVEGFR1-Fc coated chip; dashed line (–), hVEGFR2-Fc coated chip; dashed line (—), hVEGFR-3-Fc coated chip. 50 nM of TTAC-0001 was injected at a flow rate of 30 μl/min. ( c ) Inhibition of TTAC-0001 in binding VEGF isoforms to VEGFR-2. Closed rectangular represents VEGF165. Closed circle represents VEGF-C and Open circle represents VEGF-D. ( d ) Immunohistochemistry showed positive staining of TTAC-0001 in endothelial cells of mouse aorta and mouse brain bEND.3 cells from Balb/c mice. HUVECs and human GBM tumor tissues were used as controls. Triangle represents stained regions of each cell.
    Figure Legend Snippet: Measurement of the specificity of TTAC-0001. ( a ) Binding of TTAC-0001 to VEGF isoforms. Black bar represents VEGF-165. Gray bar represents VEGF-C and blank bar represents VEGF-D. hIgG and bevacizumab were used as controls. ( b ) Specificity measurement of TTAC-0001 on VEGFRs by Biacore. Line, hVEGFR1-Fc coated chip; dashed line (–), hVEGFR2-Fc coated chip; dashed line (—), hVEGFR-3-Fc coated chip. 50 nM of TTAC-0001 was injected at a flow rate of 30 μl/min. ( c ) Inhibition of TTAC-0001 in binding VEGF isoforms to VEGFR-2. Closed rectangular represents VEGF165. Closed circle represents VEGF-C and Open circle represents VEGF-D. ( d ) Immunohistochemistry showed positive staining of TTAC-0001 in endothelial cells of mouse aorta and mouse brain bEND.3 cells from Balb/c mice. HUVECs and human GBM tumor tissues were used as controls. Triangle represents stained regions of each cell.

    Techniques Used: Binding Assay, Chromatin Immunoprecipitation, Injection, Flow Cytometry, Inhibition, Immunohistochemistry, Staining, Mouse Assay

    Related Articles

    Incubation:

    Article Title: Role of VEGF Receptors in Normal and Psoriatic Human Keratinocytes: Evidence from Irradiation with Different UV Sources
    Article Snippet: .. After being rinsed three times for 10 min with TBST, the membrane was incubated with the primary antibodies(1∶500–1∶2000) to P-VEGFR-2 (Tyr1175) (Cell Signaling Technology, Beverly, MA, USA), VEGF165, VEGFR-1, VEGFR-2, NRP-1, P-VEGFR-1 (Y1213), Bcl-2, cleaved Caspase-3, Akt, P-Akt (Thr308), ERK1/2, P-ERK1/2 (Thr202/Tyr204), GAPDH (R & D Systems, Minneapolis, MN, USA), and overnight at 4°C in TBST containing 1% BSA. .. Blots were then washed 3 times (each 10 min) in TBST and were incubated for 2 h with horseradish peroxidase-conjugated secondary IgG antibody (Jackson, West Grove, PA, USA).

    Article Title: Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute
    Article Snippet: Proteins were run on a sodium dodecyl sulfate polyacrylamide electrophoresis gel and transferred to a polyvinylidene fluoride membrane. .. After rinsing the membrane in PBS (3×), VEGF165 antibody (1:2,000; R & D Systems, Minneapolis, MN, USA) was added and incubated for 1 hour. .. After rinsing with Tris-buffered saline–Tween (3×), goat anti-rabbit secondary antibody (1:1,000; Jackson, West Grove, PA, USA) was added and incubated at room temperature for 1 hour.

    Article Title: Suppression of Human Tenon Fibroblast Cell Proliferation by Lentivirus-Mediated VEGF Small Hairpin RNA
    Article Snippet: Aliquots of total proteins were resolved using SDS polyacrylamide gel electrophoresis and were then transferred onto immobilon P membrane using an SD semidry transfer apparatus according to the manufacturer's instructions. .. Membranes were incubated with polyclonal antihuman VEGF antibody (Cat. #AF-293-NA, R & D Systems, USA) and monoclonal antihuman β -actin (1 : 10,000 dilution; Sigma). .. Peroxidase-conjugated secondary antibodies were used as secondary detection reagents with an enhanced chemiluminescence kit (Cat. #20-500, Biological Industries, Israel).

    Article Title: Role of VEGF Receptors in Normal and Psoriatic Human Keratinocytes: Evidence from Irradiation with Different UV Sources
    Article Snippet: .. Then, the sections were incubated overnight at 4°C with 20 µg/ml primary antibodies against VEGF165, VEGFR-1, VEGFR-2, NRP-1 (R & D Systems, Minneapolis, MN, USA), and P-VEGFR-2 (Tyr1175) (Cell Signaling Technology, Beverly, MA, USA) diluted with PBS containing 1% BSA. .. After 3 washes with PBS, the sections were incubated with Rhodamine or FITC-conjugated secondary antibodies (Dako-Cytomation, Denmark A/S, Glostrup, Denmark) that was diluted 1∶200 with PBS containing 1% BSA for 2 h in the dark.

    Neutralization:

    Article Title: Platelet-Rich Plasma Promotes the Proliferation of Human Muscle Derived Progenitor Cells and Maintains Their Stemness
    Article Snippet: Neutralization Assay 2×103 hMDPCs were seeded onto a 48 well plate in BM. .. Twenty-four hours later, the media were changed to different neutralization-antibody-conditioned media, which were prepared by adding the following neutralizing antibodies (Abs) to the culture media supplemented with 10% PRP, and gently agitated at 4°C for 1 h: neutralization Abs against VEGF (AF-293-NA, neutralizing the biological activity of VEGF165 and VEGF121 ; R & D System), PDGF (AB-20-NA, neutralizing the biological activity of natural human PDGF including PDGF-AB, BB, & AA; R & D Systems), or TGF-β1 (TB21; neutralizing the biological activity of human TGF-β1; Abcam); and low endotoxin isotype control Abs (0109-14, goat IgG, SouthernBiotech; 011-001-297, rabbit IgG, Rockland; 400124, mouse IgG, Biolegend) were also added as controls. .. The effects that the neutralized PRP had on the growth of the hMDPCs were determined using the DNA assay described in the Proliferation Assay section.

    Activity Assay:

    Article Title: Platelet-Rich Plasma Promotes the Proliferation of Human Muscle Derived Progenitor Cells and Maintains Their Stemness
    Article Snippet: Neutralization Assay 2×103 hMDPCs were seeded onto a 48 well plate in BM. .. Twenty-four hours later, the media were changed to different neutralization-antibody-conditioned media, which were prepared by adding the following neutralizing antibodies (Abs) to the culture media supplemented with 10% PRP, and gently agitated at 4°C for 1 h: neutralization Abs against VEGF (AF-293-NA, neutralizing the biological activity of VEGF165 and VEGF121 ; R & D System), PDGF (AB-20-NA, neutralizing the biological activity of natural human PDGF including PDGF-AB, BB, & AA; R & D Systems), or TGF-β1 (TB21; neutralizing the biological activity of human TGF-β1; Abcam); and low endotoxin isotype control Abs (0109-14, goat IgG, SouthernBiotech; 011-001-297, rabbit IgG, Rockland; 400124, mouse IgG, Biolegend) were also added as controls. .. The effects that the neutralized PRP had on the growth of the hMDPCs were determined using the DNA assay described in the Proliferation Assay section.

    Article Title: Secretome of apoptotic peripheral blood cells (APOSEC) confers cytoprotection to cardiomyocytes and inhibits tissue remodelling after acute myocardial infarction: a preclinical study
    Article Snippet: VEGF, IL-8, ENA-78 and MMP9 were selected based on the higher expression levels over controls and their known cytoprotective properties. .. The activity of these selected factors was blocked using the recommended concentration of the neutralizing antibodies (2 μg/ml anti-VEGF: AF-293-NA, 2 μg/ml anti-IL-8: MAB208, R & D Systems; 2 μg/ml anti-ENA-78: AB-254-PB and 2 μg/ml anti-MMP9: MAB911; all antibodies obtained from R & D Systems, USA). .. Mouse and goat isotype antibodies were used as controls (Mouse IgG (Clone 11711), MAB002, Goat IgG, AB-108-C, R & D Systems).

    Concentration Assay:

    Article Title: Secretome of apoptotic peripheral blood cells (APOSEC) confers cytoprotection to cardiomyocytes and inhibits tissue remodelling after acute myocardial infarction: a preclinical study
    Article Snippet: VEGF, IL-8, ENA-78 and MMP9 were selected based on the higher expression levels over controls and their known cytoprotective properties. .. The activity of these selected factors was blocked using the recommended concentration of the neutralizing antibodies (2 μg/ml anti-VEGF: AF-293-NA, 2 μg/ml anti-IL-8: MAB208, R & D Systems; 2 μg/ml anti-ENA-78: AB-254-PB and 2 μg/ml anti-MMP9: MAB911; all antibodies obtained from R & D Systems, USA). .. Mouse and goat isotype antibodies were used as controls (Mouse IgG (Clone 11711), MAB002, Goat IgG, AB-108-C, R & D Systems).

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    R&D Systems recombinant human vegf165
    Endothelial cell proliferation by VEGF189 and <t>VEGF165</t> produced by selected MDA-MB-231 cells. HUVECs (5 × 10 3 /well) were incubated with the conditioned medium of selected V165 and V189 clones, as described in Materials and Methods; the proliferation
    Recombinant Human Vegf165, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human vegf165/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human vegf165 - by Bioz Stars, 2021-04
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    97
    R&D Systems elisa kit
    Supernatants of MSCs in each group (Control group, BMP group, VEGF group and BMP+VEGF group) were collected at 1, 4, and 8 weeks after transfection and measured with <t>ELISA</t> kits specific for <t>BMP2</t> (A) and VEGF165 (B) . Results are shown as mean ±SD (n = 4 for each group).
    Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/R&D Systems
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2021-04
    97/100 stars
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    93
    R&D Systems vegf 165
    APLNR regulates  CXCR4  expression through miR-139-5p. ( a , b ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent  APLNR  knockdown. mRNA ( a ) and protein levels ( b ) are shown. ( c ) CXCR4 expression in response to  AGO2  knockdown. ( d ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent  AGO2  knockdown. ( e ) Determination of miR-139-5p levels in FACS sorted ECs from P5 retinas of  Aplnr −/− ,  Apln −/−  mice and respective littermates.  n= 3 retinas per genotype. ( f ) CXCR4 expression in HUVECs overexpressing miR-139-5p mimic or non-targeting control anti-miR. ( g ) Expression levels of miR-139-5p in response to shear stress, with or without concurrent  APLNR  knockdown. ( h ) CXCR4 expression in HUVECs in response to shear stress exposure, with or without concurrent miR-139-5p inhibition via anti-miR. ( i ) Sprouting assay using HUVEC covered beads transduced with miR-139-5p or control lentivirus in response to SDF-1α or VEGF 165. Scale bar, 175 μm. ( j ) Migration assay of HUVECs transduced with miR-139-5p or control lentivirus in response to SDF-1α. Scale bar, 200 μm. * P
    Vegf 165, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf 165/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf 165 - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    N/A
    The Human VEGF165 Antibody from R D Systems is a goat polyclonal antibody to VEGF This antibody reacts with human The Human VEGF165 Antibody has been validated for the following
      Buy from Supplier

    Image Search Results


    Endothelial cell proliferation by VEGF189 and VEGF165 produced by selected MDA-MB-231 cells. HUVECs (5 × 10 3 /well) were incubated with the conditioned medium of selected V165 and V189 clones, as described in Materials and Methods; the proliferation

    Journal: The American Journal of Pathology

    Article Title: Overexpression of Vascular Endothelial Growth Factor 189 in Breast Cancer Cells Leads to Delayed Tumor Uptake with Dilated Intratumoral Vessels

    doi: 10.2353/ajpath.2008.070181

    Figure Lengend Snippet: Endothelial cell proliferation by VEGF189 and VEGF165 produced by selected MDA-MB-231 cells. HUVECs (5 × 10 3 /well) were incubated with the conditioned medium of selected V165 and V189 clones, as described in Materials and Methods; the proliferation

    Article Snippet: Recombinant human VEGF165 (R & D Systems) and VEGF189 were used as controls.

    Techniques: Produced, Multiple Displacement Amplification, Incubation, Clone Assay

    Generation of MDA-MB-231 Cells Stably Expressing VEGF189 or VEGF165 Protein

    Journal: The American Journal of Pathology

    Article Title: Overexpression of Vascular Endothelial Growth Factor 189 in Breast Cancer Cells Leads to Delayed Tumor Uptake with Dilated Intratumoral Vessels

    doi: 10.2353/ajpath.2008.070181

    Figure Lengend Snippet: Generation of MDA-MB-231 Cells Stably Expressing VEGF189 or VEGF165 Protein

    Article Snippet: Recombinant human VEGF165 (R & D Systems) and VEGF189 were used as controls.

    Techniques: Multiple Displacement Amplification, Stable Transfection, Expressing

    Growth curves of MDA-overexpressing VEGF189 and VEGF165 tumors induced in SCID mice. Cells (5 × 10 6 ) were subcutaneously injected in SCID mice as described in Materials and Methods. A: Tumors were generated from V189-13 or V165-42 clone, or cV

    Journal: The American Journal of Pathology

    Article Title: Overexpression of Vascular Endothelial Growth Factor 189 in Breast Cancer Cells Leads to Delayed Tumor Uptake with Dilated Intratumoral Vessels

    doi: 10.2353/ajpath.2008.070181

    Figure Lengend Snippet: Growth curves of MDA-overexpressing VEGF189 and VEGF165 tumors induced in SCID mice. Cells (5 × 10 6 ) were subcutaneously injected in SCID mice as described in Materials and Methods. A: Tumors were generated from V189-13 or V165-42 clone, or cV

    Article Snippet: Recombinant human VEGF165 (R & D Systems) and VEGF189 were used as controls.

    Techniques: Multiple Displacement Amplification, Mouse Assay, Injection, Generated

    In Vitro Tube Formation Assay Using Human Umbilical Vein Endothelial Cells (HUVECs) on a Three-Dimensional Gel Consisting of Diluted Matrigel The cells were treated with Apt01 (10 μM), Apt02 (10 μM), or VEGF165 (10 ng/mL, 0.26 nM) for 24 h. (A) Representative images of tube formation of HUVECs on Matrigel, which are treated by aptamers or VEGF165. Scale bars, 1 mm. (B) Time course of mesh number in the images of the HUVEC networks at 4, 7, and 24 h. Error bars represent standard deviation of the mean (n = 3 plots). (C–E) Boxplot showing mesh number in the images of the HUVEC networks at (C) 4, (D) 7, and (E) 24 h (n = 3 plots). *p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Binding and Structural Properties of DNA Aptamers with VEGF-A-Mimic Activity

    doi: 10.1016/j.omtn.2019.12.034

    Figure Lengend Snippet: In Vitro Tube Formation Assay Using Human Umbilical Vein Endothelial Cells (HUVECs) on a Three-Dimensional Gel Consisting of Diluted Matrigel The cells were treated with Apt01 (10 μM), Apt02 (10 μM), or VEGF165 (10 ng/mL, 0.26 nM) for 24 h. (A) Representative images of tube formation of HUVECs on Matrigel, which are treated by aptamers or VEGF165. Scale bars, 1 mm. (B) Time course of mesh number in the images of the HUVEC networks at 4, 7, and 24 h. Error bars represent standard deviation of the mean (n = 3 plots). (C–E) Boxplot showing mesh number in the images of the HUVEC networks at (C) 4, (D) 7, and (E) 24 h (n = 3 plots). *p

    Article Snippet: HUVECs (1.1 × 105 cells/well) were placed into 24-well flat-bottomed plates pre-coated with Matrigel and then incubated with aptamers (10 μM) or VEGF165 (Recombinant Human VEGF 165 Protein, Cat. No. 293-VE [R & D Systems, USA]; 10 ng/mL, 0.26 nM) for 24 h. In the experiment of dose dependency, the Apt02 concentration of more than 10 μM showed the significant difference from control group (data not shown).

    Techniques: In Vitro, Tube Formation Assay, Standard Deviation

    Supernatants of MSCs in each group (Control group, BMP group, VEGF group and BMP+VEGF group) were collected at 1, 4, and 8 weeks after transfection and measured with ELISA kits specific for BMP2 (A) and VEGF165 (B) . Results are shown as mean ±SD (n = 4 for each group).

    Journal: International Journal of Molecular Sciences

    Article Title: Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro

    doi: 10.3390/ijms12031744

    Figure Lengend Snippet: Supernatants of MSCs in each group (Control group, BMP group, VEGF group and BMP+VEGF group) were collected at 1, 4, and 8 weeks after transfection and measured with ELISA kits specific for BMP2 (A) and VEGF165 (B) . Results are shown as mean ±SD (n = 4 for each group).

    Article Snippet: Enzyme Linked Immunoabsorbent Assay (ELISA) The in vitro hBMP2 and hVEGF165 production in each group was quantified in culture supernatant at 1, 4, and 8 weeks after transduction using an ELISA kit (BMP2 ELISA kit, R & D system; VEGF165 ELISA kit, BIOSOURCE) according to the manufacturer’s instructions.

    Techniques: Transfection, Enzyme-linked Immunosorbent Assay

    APLNR regulates  CXCR4  expression through miR-139-5p. ( a , b ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent  APLNR  knockdown. mRNA ( a ) and protein levels ( b ) are shown. ( c ) CXCR4 expression in response to  AGO2  knockdown. ( d ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent  AGO2  knockdown. ( e ) Determination of miR-139-5p levels in FACS sorted ECs from P5 retinas of  Aplnr −/− ,  Apln −/−  mice and respective littermates.  n= 3 retinas per genotype. ( f ) CXCR4 expression in HUVECs overexpressing miR-139-5p mimic or non-targeting control anti-miR. ( g ) Expression levels of miR-139-5p in response to shear stress, with or without concurrent  APLNR  knockdown. ( h ) CXCR4 expression in HUVECs in response to shear stress exposure, with or without concurrent miR-139-5p inhibition via anti-miR. ( i ) Sprouting assay using HUVEC covered beads transduced with miR-139-5p or control lentivirus in response to SDF-1α or VEGF 165. Scale bar, 175 μm. ( j ) Migration assay of HUVECs transduced with miR-139-5p or control lentivirus in response to SDF-1α. Scale bar, 200 μm. * P

    Journal: Nature Communications

    Article Title: MicroRNA 139-5p coordinates APLNR-CXCR4 crosstalk during vascular maturation

    doi: 10.1038/ncomms11268

    Figure Lengend Snippet: APLNR regulates CXCR4 expression through miR-139-5p. ( a , b ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent APLNR knockdown. mRNA ( a ) and protein levels ( b ) are shown. ( c ) CXCR4 expression in response to AGO2 knockdown. ( d ) CXCR4 expression in response to shear stress exposure in HUVECs, with or without concurrent AGO2 knockdown. ( e ) Determination of miR-139-5p levels in FACS sorted ECs from P5 retinas of Aplnr −/− , Apln −/− mice and respective littermates. n= 3 retinas per genotype. ( f ) CXCR4 expression in HUVECs overexpressing miR-139-5p mimic or non-targeting control anti-miR. ( g ) Expression levels of miR-139-5p in response to shear stress, with or without concurrent APLNR knockdown. ( h ) CXCR4 expression in HUVECs in response to shear stress exposure, with or without concurrent miR-139-5p inhibition via anti-miR. ( i ) Sprouting assay using HUVEC covered beads transduced with miR-139-5p or control lentivirus in response to SDF-1α or VEGF 165. Scale bar, 175 μm. ( j ) Migration assay of HUVECs transduced with miR-139-5p or control lentivirus in response to SDF-1α. Scale bar, 200 μm. * P

    Article Snippet: The fibrinogen/bead solution was allowed to clot for 5 min at room temperature and then at 37 °C in 5% CO2 for 15 min. EGM-2 containing 50 ng ml−1 SDF-1α (SRP3252, Sigma-Aldrich), or 20 ng ml−1 VEGF 165 (293-VE, R & D Systems) was used.

    Techniques: Expressing, FACS, Mouse Assay, Inhibition, Transduction, Migration