vegf  (WuXi AppTec)

 
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  • 93
    Name:
    VEGF Antibody C term
    Description:
    Purified Rabbit Polyclonal Antibody Pab Rabbit Ig
    Catalog Number:
    ap6290b
    Price:
    [109.0, 325.0]
    Applications:
    Western Blot,IHC (Paraffin)
    Host:
    Rabbit
    Immunogen:
    This VEGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human VEGF Antibody.
    Size:
    80 ul 400 ul
    Category:
    Antibody
    Antibody Type:
    Primary antibody
    Isotype:
    Ig
    Preparation:
    This VEGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C terminal region of human VEGF Antibody
    Reactivity:
    Human
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    Structured Review

    WuXi AppTec vegf
    Purified Rabbit Polyclonal Antibody Pab Rabbit Ig
    https://www.bioz.com/result/vegf/product/WuXi AppTec
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2020-08
    93/100 stars

    Images

    Related Articles

    Immunodetection:

    Article Title: Development of an aptamer-based affinity purification method for vascular endothelial growth factor
    Article Snippet: .. SDS-PAGE gels were either silver stained or further used for western blot analysis using WesternBreeze Chromogenic Immunodetection Kit (Life Technologies Inc., CA, USA) and anti VEGF antibody (Abgent, #AJ1813). .. PageRuler Unstained Protein Ladder and PageRuler Plus Prestained Protein Ladder (Thermo Scientific, MA, USA) were used as molecular weight markers.

    Purification:

    Article Title: Recombinant human endostatin combined with radiotherapy inhibits colorectal cancer growth
    Article Snippet: .. The protein was subsequently transferred onto a PVDF membrane (Roche Applied Science, Shanghai, China), which was blocked for 1 h using 5% defatted milk diluted in Tris buffered saline Tween (TBST) before being incubated with a primary antibody to VEGF (purified rabbit polyclonal antibody, diluted 1:200, WuXi AppTec, AP6290b-400, Suzhou, China) overnight at 4 °C. .. The membranes were then incubated with a goat anti-rabbit IgG (H + L) HRP-conjugated secondary antibody (1:3000, LK2001, Sungene Biotech, Tianjin, China) for 1 h at room temperature.

    Incubation:

    Article Title: Immunoglobulin-like transcript 4 promotes tumor progression and metastasis and up-regulates VEGF-C expression via ERK signaling pathway in non-small cell lung cancer
    Article Snippet: .. Subsequently, the membrane was blocked and incubated overnight at 4°C with the primary antibody including anti-ILT4 mAb (1: 400, Abgent, San Diego, CA, USA), anti-p-ERK pAb (1:1000, Epitomics, Burlingame, CA, USA), anti-ERK pAb (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-p-STAT3 pAb (1:1000, Epitomics), anti-JNK pAb (1:1000, Sangon Biotech, Shanghai, China), anti-p-p38MAPK pAb (1:1000, Sangon Biotech, Shanghai, China), and anti-VEGF-C pAb (1:1000, Abgent). .. Afterwards, the blots were labeled for 1 h with HRP-conjugated secondary antibodies (1:5000, Proteintech Group, Inc., Wuhan, China).

    Article Title: Immunoglobulin-like transcript 4 promotes tumor progression and metastasis and up-regulates VEGF-C expression via ERK signaling pathway in non-small cell lung cancer
    Article Snippet: .. Subsequently, the sections were incubated with the primary antibodies against anti–ILT4 mAb (1:200, Abgent) (1:200, R & D Systems, Abingdon, UK) or anti-VEGF-C pAb (1:100, Abgent). .. Sections incubated with normal mouse or rabbit IgG instead of primary antibodies were used as negative control.

    Article Title: Recombinant human endostatin combined with radiotherapy inhibits colorectal cancer growth
    Article Snippet: .. The protein was subsequently transferred onto a PVDF membrane (Roche Applied Science, Shanghai, China), which was blocked for 1 h using 5% defatted milk diluted in Tris buffered saline Tween (TBST) before being incubated with a primary antibody to VEGF (purified rabbit polyclonal antibody, diluted 1:200, WuXi AppTec, AP6290b-400, Suzhou, China) overnight at 4 °C. .. The membranes were then incubated with a goat anti-rabbit IgG (H + L) HRP-conjugated secondary antibody (1:3000, LK2001, Sungene Biotech, Tianjin, China) for 1 h at room temperature.

    Western Blot:

    Article Title: Development of an aptamer-based affinity purification method for vascular endothelial growth factor
    Article Snippet: .. SDS-PAGE gels were either silver stained or further used for western blot analysis using WesternBreeze Chromogenic Immunodetection Kit (Life Technologies Inc., CA, USA) and anti VEGF antibody (Abgent, #AJ1813). .. PageRuler Unstained Protein Ladder and PageRuler Plus Prestained Protein Ladder (Thermo Scientific, MA, USA) were used as molecular weight markers.

    Staining:

    Article Title: Development of an aptamer-based affinity purification method for vascular endothelial growth factor
    Article Snippet: .. SDS-PAGE gels were either silver stained or further used for western blot analysis using WesternBreeze Chromogenic Immunodetection Kit (Life Technologies Inc., CA, USA) and anti VEGF antibody (Abgent, #AJ1813). .. PageRuler Unstained Protein Ladder and PageRuler Plus Prestained Protein Ladder (Thermo Scientific, MA, USA) were used as molecular weight markers.

    SDS Page:

    Article Title: Development of an aptamer-based affinity purification method for vascular endothelial growth factor
    Article Snippet: .. SDS-PAGE gels were either silver stained or further used for western blot analysis using WesternBreeze Chromogenic Immunodetection Kit (Life Technologies Inc., CA, USA) and anti VEGF antibody (Abgent, #AJ1813). .. PageRuler Unstained Protein Ladder and PageRuler Plus Prestained Protein Ladder (Thermo Scientific, MA, USA) were used as molecular weight markers.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 93
    WuXi AppTec vegf
    Vegf, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/WuXi AppTec
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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