vegf (Abcam)
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Recombinant VEGF Receptor 3 mouse
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ab205902
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1) Product Images from "Mechanisms of tumor-promoting activities of nicotine in lung cancer: synergistic effects of cell membrane and mitochondrial nicotinic acetylcholine receptors"
Article Title: Mechanisms of tumor-promoting activities of nicotine in lung cancer: synergistic effects of cell membrane and mitochondrial nicotinic acetylcholine receptors
Journal: BMC Cancer
doi: 10.1186/s12885-015-1158-4

Figure Legend Snippet: Synergistic effects of combinations of nicotine (N) with EGF (E), IGF-I (I) or VEGF (V) on proliferation of different types of lung cells. Alive, ie, TBD-negative, cells seeded at a density of 1 × 10 4 per well of a 96-well plate were counted after 24 h of incubation in the absence (intact control) or presence of the optimal doses of nicotine (see text) and 10 ng/ml of each test GF. Some cells were exposed to 20 μM HC-3 ( H ) ± nicotine and some — to nicotine/GF combinations in the presence of αBtx ( B; 1 μM) and/or Mec ( M; 50 μM). All values significantly (p
Techniques Used: Incubation

Figure Legend Snippet: Roles of individual cm-nAChR subtypes in implementing synergy of nicotine with GFs. The SW900 cells transfected with either shRNA-NC (control; taken as 100%) or anti-nAChR shRNA were incubated for 24 h in the absence (1) or presence of combinations of 0.1 μM nicotine with 10 ng/ml of EGF (2) , IGF-I (3) or VEGF (4) , and then subjected to direct counting (TBD-negative cells only). Data are mean + SD from a triplicate sample. Asterisk = p
Techniques Used: Transfection, shRNA, Incubation
2) Product Images from "IQGAP1 Regulates Adult Neural Progenitors In Vivo and Vascular Endothelial Growth Factor-Triggered Neural Progenitor Migration In Vitro"
Article Title: IQGAP1 Regulates Adult Neural Progenitors In Vivo and Vascular Endothelial Growth Factor-Triggered Neural Progenitor Migration In Vitro
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.0830-07.2007

Figure Legend Snippet: IQGAP1 regulates VEGF-dependent neural progenitor cell migration. A , Reverse transcription-PCR (RT) analysis performed on wild-type and Iqgap1 -null mutant neurospheres reveal expression of both VEGF receptors Flk-1 (VEGF-R2) and Flt-1 (VEGF-R1). NP, Neural progenitors. B , Phase-contrast reconstitution of time-lapse imaging of wild-type and Iqgap1 -null mutant-derived neurospheres in response to VEGF (20 ng/ml) stimulation. C , Quantification of the average migration distance ( n = 10) of wild-type (WT) and Iqgap1 -null (KO) neurosphere cells after VEGF stimulation.
Techniques Used: Migration, Polymerase Chain Reaction, Mutagenesis, Expressing, Imaging, Derivative Assay

Figure Legend Snippet: Analysis of IQGAP1 partners in wild-type neurospheres stimulated with VEGF. A , VEGF stimulation enhances colocalization of IQGAP1 with Rac1 at the cell membrane. Unstimulated (control) or VEGF-stimulated neurospheres (VEGF) were double immunostained with anti-IQGAP1 (green) and anti-Rac1 (red) antibodies. Scale bars, 10 μm. B , Characterization of IQGAP1 immunoprecipitates (IP) from neurospheres stimulated with VEGF. Total neurosphere extracts (lane 1) and IQGAP1 immunoprecipitates from neurospheres not stimulated (lane 2) or stimulated with VEGF for 10 min (lanes 3) or 30 min (lanes 4) were analyzed by Western blot with polyclonal IQGAP1 and monoclonal Cdc42, Rac1, and Lis1 antibodies as indicated. Lane 5 is a control (Ctl) lane corresponding to the IQGAP1 immunoprecipitate from iqgap 1-null neurospheres.
Techniques Used: Western Blot, CTL Assay
3) Product Images from "Overexpression of hypoxia-inducible factor-1α and vascular endothelial growth factor in sacral giant cell tumors and the correlation with tumor microvessel density"
Article Title: Overexpression of hypoxia-inducible factor-1α and vascular endothelial growth factor in sacral giant cell tumors and the correlation with tumor microvessel density
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2014.1971

Figure Legend Snippet: Representative immunohistochemical staining for HIF-1α, VEGF and CD34 expression in (A, C and E) sacral GCT samples, respectively, and (B, D and F) normal sacral tissues, respectively (magnification, ×200; light microscopy). HIF-1α, hypoxia-inducible factor 1α; VEGF, vascular endothelial growth factor; GCT, giant cell tumor.
Techniques Used: Immunohistochemistry, Staining, Expressing, Light Microscopy

Figure Legend Snippet: Overexpression of HIF-1α and VEGF correlates with an increased MVD value in sacral GCT specimens. Immunohistochemistry scores of (A) HIF-1α and (B) VEGF overexpression in the sacral GCT specimens. (C) Increased MVD values were observed in sacral GCT specimens. Correlations between the relative (D) HIF-1α and (E) VEGF expression levels with the MVD value. P
Techniques Used: Over Expression, Immunohistochemistry, Expressing

Figure Legend Snippet: Representative western blot analysis for HIF-1α, VEGF and CD34 expression in sacral GCT specimens. (A) HIF-1α, VEGF and CD34 protein expression levels were determined in sacral GCT and normal samples (n=22) using western blot analysis. Relative expression levels of (B) HIF-1α, (C) VEGF and (D) CD34 against GAPDH in the sacral GCT samples. * P
Techniques Used: Western Blot, Expressing
4) Product Images from "Administered circulating microparticles derived from lung cancer patients markedly improved angiogenesis, blood flow and ischemic recovery in rat critical limb ischemia"
Article Title: Administered circulating microparticles derived from lung cancer patients markedly improved angiogenesis, blood flow and ischemic recovery in rat critical limb ischemia
Journal: Journal of Translational Medicine
doi: 10.1186/s12967-015-0381-8

Figure Legend Snippet: Proposed mechanisms underlying the positive therapeutic effects of circulating microparticles on critical limb ischemia. Lc-MPs = lung cancer-derived circulating microparticles; NO = nitric oxide; VEGFR2 = vascular endothelial growth factor receptor 2; SDF-1α = stromal cell-derived factor 1 alpha; ANGPT = angiopoietin; eNOS = endothelial nitric oxide synthase; HGF = hepatocyte growth factor; vWF = von Willebrand factor; IFBF = Ratio of ischemic to normal blood flow; PARP = poly (ADP-ribose) polymerase; TGF-β = transforming growth factor beta; BMP = bone morphogenesis protein.
Techniques Used: Derivative Assay, Flow Cytometry
5) Product Images from "Cellular and Matrix Response of the Mandibular Condylar Cartilage to Botulinum Toxin"
Article Title: Cellular and Matrix Response of the Mandibular Condylar Cartilage to Botulinum Toxin
Journal: PLoS ONE
doi: 10.1371/journal.pone.0164599

Figure Legend Snippet: Decreased expression of SMAD1/5/8 and VEFG in the Botox injected side MCC and subchondral bone. Immunohistochemistry for SMAD1/5/8 in sagittal sections of control (A) and Botox (B) injected side condyles. VEGF Immunohistochemistry in sections of control (C) and Botox (D) injected side condyles. Scale bar = 500μm.
Techniques Used: Expressing, Injection, Immunohistochemistry
6) Product Images from "Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine"
Article Title: Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine
Journal: Nature biomedical engineering
doi: 10.1038/s41551-017-0082

Figure Legend Snippet: ELISA and lateral flow strips with EASE ( a ) Schematic illustration of the signal enhancement process. Upon target detection, a layer of PDA is coated around the target molecule, which allows a large number of HRP to adsorb. These HRP molecules, in turn, catalyze the substrate (TMB) conversion at a significantly enhanced rate. ( b ) Visual assessment of the detection sensitivity of ELISA-EASE using mouse IgG as a model target in comparison with conventional ELISA. Colored solutions are visualized in ELISA-EASE wells at target concentrations as low as 10 −13 g ml −1 , while the conventional assay only produces detectable colors at 10 −8 to 10 −9 g ml −1 concentration range. ( c ) Quantitative comparison using values obtained from a plate reader shows the assay response over the full target concentration range (left) and a zoomed-in range close to the essays’ LODs (right). Improvement of approximately 3 orders of magnitude is seen. Error bars, s.d. over three replicates. ( d ) ELISA working curves for four targets, HIV p24, KLK3, CRP, VEGF, with or without EASE. Error bars, s.d. over three replicates. ( e ) The average of LOD improvements for all four targets is around 1,200 fold. ( f ) Strip tests of HIV p24 with or without EASE. Positive signals (indicated by the red arrow) are observed at 10 ng ml −1 and 10 pg ml −1 for the EASE-strips, but the conventional strips can only detect down to 10 ng ml −1 . Each strip represents three replicates.
Techniques Used: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Concentration Assay, Stripping Membranes
7) Product Images from "Endothelial progenitor cells improve the therapeutic effect of mesenchymal stem cell sheets on irradiated bone defect repair in a rat model"
Article Title: Endothelial progenitor cells improve the therapeutic effect of mesenchymal stem cell sheets on irradiated bone defect repair in a rat model
Journal: Journal of Translational Medicine
doi: 10.1186/s12967-018-1517-4

Figure Legend Snippet: Subcutaneous osteogenesis of cell sheets. Representative H E (×200) and immunohistochemical staining images (×200) of BMP-2, OCN, VEGF and CD31. New bone formation (NB), osteoid (OS), blood vessels (asterisk) are indicated in the images. Black arrows indicate positive staining cells, red arrows indicate positive staining bone matrix
Techniques Used: Immunohistochemistry, Staining

Figure Legend Snippet: Osteogenic genes and proteins expression of cell sheets after osteogenic differentiation. a Gene expression of Runx2, Alp, Bmp - 2, Ocn and Vegf after 0, 3 and 7 days of osteogenic induction. b Western blot analysis of RUNX2, ALP, BMP-2, OCN, VEGF and GAPDH after 7 days of osteogenic induction and the quantitative analysis of the protein bands. Data are shown as mean ± SD and analyzed by Student t-test, n = 3; *p
Techniques Used: Expressing, ALP Assay, Western Blot
8) Product Images from "Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients"
Article Title: Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients
Journal: Oncology Letters
doi: 10.3892/ol.2015.3960

Figure Legend Snippet: Detection of cathepsins B, D, G, K, L and V as well as RECK and VEGF proteins in breast cancer tissue specimens using immunohistochemistry. Representative images were selected to reflect the expression of (A and B) cathepsin B, (C and D) cathepsin C, (E and F) cathepsin D, (G and H) cathepsin K, (I and J) cathepsin L, (K and L) RECK, (M and N) cathepsin V expression and (O and P) VEGF in breast cancer tissues. Magnification, ×200. RECK, reversion-inducing cysteine-rich protein with Kazal motifs; VEGF, vascular endothelial growth factor.
Techniques Used: Immunohistochemistry, Expressing
9) Product Images from "IL13Rα2 siRNA inhibited cell proliferation, induced cell apoptosis, and suppressed cell invasion in papillary thyroid carcinoma cells"
Article Title: IL13Rα2 siRNA inhibited cell proliferation, induced cell apoptosis, and suppressed cell invasion in papillary thyroid carcinoma cells
Journal: OncoTargets and therapy
doi: 10.2147/OTT.S153703

Figure Legend Snippet: Higher expression level of IL13Rα2 and VEGF were detected in PTC tissues. Notes: Higher mRNA levels of IL13Rα2 ( A ) and VEGF ( B ) were measured in PTC tissue samples (n=45) by using real-time PCR compared with paracancerous tissues (n=45). ** P
Techniques Used: Expressing, Real-time Polymerase Chain Reaction

Figure Legend Snippet: IL13Rα2 siRNA inhibited cell invasion in TPC-1 cells. Notes: ( A ) Downregulation of invasion rate was detected in TPC-1 and ARO cells after the transfection with IL13Rα2 siRNAs (siRNA-1 and siRNA-2). ( B ) Expression levels of VEGF, VEGFR2, MMP2, and MMP9 in TPC-1 and ARO cells were measured by using Western blot analysis. ** P
Techniques Used: Transfection, Expressing, Western Blot
10) Product Images from "VEGF promotes gastric cancer development by upregulating CRMP4"
Article Title: VEGF promotes gastric cancer development by upregulating CRMP4
Journal: Oncotarget
doi: 10.18632/oncotarget.7717

Figure Legend Snippet: Immunohistochemical analysis of the expression levels of VEGF, VEGFR2 and CRMP4 in tumor sections from gastric cancer patients A. Immunohistochemical detection of VEGF, VEGFR2, and CRMP4 expression levels in tumor and tumor-adjacent tissues collected from gastric cancer patients. B. The relative intensities of VEGF, VEGFR2 and CRMP4 expression in 10 pairs of tumor and tumor-adjacent tissues were evaluated by IPP based on the IHC results. The results analyzed with ANOVA are expressed as means ± SD. The protein expression levels of VEGF and CRMP4 were significantly increased in gastric cancer tissues compared with tumor-adjacent tissues, whereas the VEGFR2 expression levels did not display any significant increase.
Techniques Used: Immunohistochemistry, Expressing

Figure Legend Snippet: Expression of CRMP4, VEGFR2 and VEGF in various gastric carcinoma cell lines A. The expression level of VEGF was quantified by ELISA in six gastric carcinoma cell lines. B. Western blot analysis of CRMP4 and VEGFR2 protein expression in each cell line. GADPH was used as a loading control. The expression levels of CRMP4 and VEGFR2 were significantly increased in HGC27 and SGC7901 compared with the other gastric carcinoma cell lines.
Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Figure Legend Snippet: Quantification of VEGF, VEGFR2 and CRMP4 proteins and mRNA levels in gastric cancer tissues A. Western blot analysis showing increased expression levels of CRMP4, VEGF and VEGF2. B. Relative expression of CRMP4 and VEGF by western blot analysis. C. qRT-PCR to detect the relative mRNA expression levels of CRMP4, VEGF and VEGF2 in gastric cancer and tumor-adjacent tissues. All experiments were performed in triplicate. The results from 3 pairs of specimens analyzed by ANOVA are expressed as means ± SD. * P
Techniques Used: Western Blot, Expressing, Quantitative RT-PCR
11) Product Images from "Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes"
Article Title: Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes
Journal: BMC Ophthalmology
doi: 10.1186/s12886-017-0606-0

Figure Legend Snippet: Western blotting analysis of IL6 and VEGF expression. a Colored bands of IL6 and VEGF; b Expression levels of IL6 and VEGF. The expression levels of both IL6 and VEGF increased with the duration of the stretch, reaching their peak levels at 20 h but showed significant reduction at 24 h ( p
Techniques Used: Western Blot, Expressing
12) Product Images from "P311 Deficiency Leads to Attenuated Angiogenesis in Cutaneous Wound Healing"
Article Title: P311 Deficiency Leads to Attenuated Angiogenesis in Cutaneous Wound Healing
Journal: Frontiers in Physiology
doi: 10.3389/fphys.2017.01004

Figure Legend Snippet: Effect of P311 expression on wound angiogenesis. (A) IHC staining of granulation tissues (day 5) for CD31, VEGF, and TGFβ1. (B) Western blot showed the expression of CD31, VEGF, and TGFβ1 in each group. Quantitation of the protein levels of CD31, VEGF, and TGFβ1 in each group (day 5). Levels of VEGF (C) and TGFβ1 (D) releasing during skin wound (day 5) were measured by sandwich ELISA. Data were expressed as means ± SD . n = 5 per group. * P
Techniques Used: Expressing, Immunohistochemistry, Staining, Western Blot, Quantitation Assay, Sandwich ELISA
13) Product Images from "Angiogenesis for tumor vascular normalization of Endostar on hepatoma 22 tumor-bearing mice is involved in the immune response"
Article Title: Angiogenesis for tumor vascular normalization of Endostar on hepatoma 22 tumor-bearing mice is involved in the immune response
Journal: Oncology Letters
doi: 10.3892/ol.2018.7734

Figure Legend Snippet: Effects of Endostar on the mRNA levels of (A) HIF-1α and (B) VEGF in liver tissue of hepatoma 22-bearing C57BL/6 mice. All experiments were performed at least three times. Results are presented as the mean ± standard deviation (n=6). # P
Techniques Used: Mouse Assay, Standard Deviation

Figure Legend Snippet: (A) Effects of Endostar on the expression of angiogenesis-associated proteins in mouse liver tissues during treatment. Protein from liver tissues was extracted and protein expression of (B) VEGF, (C) CD31, (D) HIF-1α, (E) MMP-2 and (F) MMP-9 was analyzed using Image-Pro Plus 6.0. All experiments were performed at least three times. Results are presented as the mean ± standard deviation (n=6). # P
Techniques Used: Expressing, Standard Deviation
14) Product Images from "High Intensity Interval and Endurance Training Have Opposing Effects on Markers of Heart Failure and Cardiac Remodeling in Hypertensive Rats"
Article Title: High Intensity Interval and Endurance Training Have Opposing Effects on Markers of Heart Failure and Cardiac Remodeling in Hypertensive Rats
Journal: PLoS ONE
doi: 10.1371/journal.pone.0121138

Figure Legend Snippet: Western blot analysis of eNOS, HIF1α, and VEGF. A. Representative blots; α-tubulin is presented as a loading control. B. Density quantifications of eNOS protein in LV of low sodium sedentary (LS-SED), high sodium SED (HS-SED), HS endurance training (HS-ET) and HS high intensity interval training (HS-HIIT), demonstrating a significant increase as a result of ET, * vs. all other groups; P
Techniques Used: Western Blot
15) Product Images from "FM19G11 inhibits O6‐methylguanine DNA‐methyltransferase expression under both hypoxic and normoxic conditions. FM19G11 inhibits O6‐methylguanine DNA‐methyltransferase expression under both hypoxic and normoxic conditions"
Article Title: FM19G11 inhibits O6‐methylguanine DNA‐methyltransferase expression under both hypoxic and normoxic conditions. FM19G11 inhibits O6‐methylguanine DNA‐methyltransferase expression under both hypoxic and normoxic conditions
Journal: Cancer Medicine
doi: 10.1002/cam4.1551

Figure Legend Snippet: Under hypoxic conditions, HIF ‐1α was expressed in both GBM ‐ XD and T98G cells. After FM 19G11 treatment, the levels of HIF ‐1α and its target genes EPO and VEGF were decreased, and the change was similar to that of MGMT
Techniques Used:
16) Product Images from "FOXP3 inhibits angiogenesis by downregulating VEGF in breast cancer"
Article Title: FOXP3 inhibits angiogenesis by downregulating VEGF in breast cancer
Journal: Cell Death & Disease
doi: 10.1038/s41419-018-0790-8

Figure Legend Snippet: VEGF is involved in the FOXP3-mediated inhibition of angiogenesis. a The tube formation activity of HUVECs that were treated with control medium, the culture supernatant of MAD-MB-231 cells (control supernatant), the culture supernatant of FOXP3-overexpressing MAD-MB-231 cells (FOXP3 supernatant), or the culture supernatant of FOXP3-overexpressing MAD-MB-231 cells supplemented with VEGF (FOXP3 supernatant + VEGF). Scale bar, 50 μm. b Quantitation of HUVEC tubulogenesis in the different groups in ( a ). HUVEC spheroid sprouting activity was determined by 3D culture. c Representative images of spheroid sprouting. Scale bar, 100 μm. d Quantitation of the cumulative sprout length (CSL) in different groups. b , d ANOVA with Tukey’s post hoc test
Techniques Used: Inhibition, Activity Assay, Quantitation Assay

Figure Legend Snippet: Inverse correlation between FOXP3 and VEGF expression in human breast cancer samples. a Representative immunohistochemical images of nuclear FOXP3 and VEGF expression in breast cancer specimens. Scale bar, 100 μm (×10) and 20 μm (×40). b A significant negative correlation between nuclear FOXP3 and VEGF expression was found in the breast cancer specimens. b Spearman test
Techniques Used: Expressing, Immunohistochemistry

Figure Legend Snippet: FOXP3 downregulates VEGF expression in breast cancer. Real-time PCR was performed to detect the transcription levels of VEGF in breast cancer cell lines with the gain or loss of FOXP3 expression. Transfection of pcDNA3.1-FOXP3 into ( a ) MCF-7, ( b ) T47D or ( c ) MAD-MB-231 cells. Transfection of FOXP3 shRNA into ( d ) MCF-7 or ( e ) T47D cells. Western blotting was performed to detect the expression of VEGF in breast cancer cell lines following the gain or loss of FOXP3 expression. Transfection of pcDNA3.1-FOXP3 into ( f ) MCF-7, ( g ) T47D or ( h ) MAD-MB-231 cells. Transfection of FOXP3 shRNA into ( i ) MCF-7 or ( j ) T47D cells. k Evaluation of FOXP3 and VEGF expression in MAD-MB-231 cells by confocal microscopy (control or FOXP3 overexpression). Scale bar, 20 μm. l Orthotopic injection of MDA-MB-231 cells was performed to generate xenografts, and adenoviruses carrying FOXP3 or control cDNA were injected into the tumors when their volume reached ~50 mm 3 ( n = 5). Serum VEGF levels of mice bearing control or FOXP3-overexpressing tumors were detected by ELISA. a – l Student’s t test
Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Transfection, shRNA, Western Blot, Confocal Microscopy, Over Expression, Injection, Multiple Displacement Amplification, Mouse Assay, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: FOXP3 is a transcriptional suppressor of VEGF. a FOXP3 suppresses VEGF promoter activity, as evaluated by dual-luciferase reporter assays. b The top panel depicts schematic diagrams of the regions amplified by the ChIP primers. The bottom panel shows the amount of DNA precipitated by either the anti-FOXP3 antibody or control IgG; the results are expressed as a percentage of the input genomic DNA from MDA-MB-231 cells. c FOXP3-mediated suppression of the VEGF promoter requires forkhead-binding motifs, as evaluated by dual-luciferase reporter assays. Relative truncation of the VEGF promoter is illustrated in the left panel. Luciferase activity was detected in cells transfected with the truncated VEGF promoter in the right panel. a , c ANOVA with Tukey’s post hoc test
Techniques Used: Activity Assay, Luciferase, Amplification, Chromatin Immunoprecipitation, Multiple Displacement Amplification, Binding Assay, Transfection
17) Product Images from "FOXP3 inhibits angiogenesis by downregulating VEGF in breast cancer"
Article Title: FOXP3 inhibits angiogenesis by downregulating VEGF in breast cancer
Journal: Cell Death & Disease
doi: 10.1038/s41419-018-0790-8

Figure Legend Snippet: VEGF is involved in the FOXP3-mediated inhibition of angiogenesis. a The tube formation activity of HUVECs that were treated with control medium, the culture supernatant of MAD-MB-231 cells (control supernatant), the culture supernatant of FOXP3-overexpressing MAD-MB-231 cells (FOXP3 supernatant), or the culture supernatant of FOXP3-overexpressing MAD-MB-231 cells supplemented with VEGF (FOXP3 supernatant + VEGF). Scale bar, 50 μm. b Quantitation of HUVEC tubulogenesis in the different groups in ( a ). HUVEC spheroid sprouting activity was determined by 3D culture. c Representative images of spheroid sprouting. Scale bar, 100 μm. d Quantitation of the cumulative sprout length (CSL) in different groups. b , d ANOVA with Tukey’s post hoc test
Techniques Used: Inhibition, Activity Assay, Quantitation Assay

Figure Legend Snippet: Inverse correlation between FOXP3 and VEGF expression in human breast cancer samples. a Representative immunohistochemical images of nuclear FOXP3 and VEGF expression in breast cancer specimens. Scale bar, 100 μm (×10) and 20 μm (×40). b A significant negative correlation between nuclear FOXP3 and VEGF expression was found in the breast cancer specimens. b Spearman test
Techniques Used: Expressing, Immunohistochemistry

Figure Legend Snippet: FOXP3 downregulates VEGF expression in breast cancer. Real-time PCR was performed to detect the transcription levels of VEGF in breast cancer cell lines with the gain or loss of FOXP3 expression. Transfection of pcDNA3.1-FOXP3 into ( a ) MCF-7, ( b ) T47D or ( c ) MAD-MB-231 cells. Transfection of FOXP3 shRNA into ( d ) MCF-7 or ( e ) T47D cells. Western blotting was performed to detect the expression of VEGF in breast cancer cell lines following the gain or loss of FOXP3 expression. Transfection of pcDNA3.1-FOXP3 into ( f ) MCF-7, ( g ) T47D or ( h ) MAD-MB-231 cells. Transfection of FOXP3 shRNA into ( i ) MCF-7 or ( j ) T47D cells. k Evaluation of FOXP3 and VEGF expression in MAD-MB-231 cells by confocal microscopy (control or FOXP3 overexpression). Scale bar, 20 μm. l Orthotopic injection of MDA-MB-231 cells was performed to generate xenografts, and adenoviruses carrying FOXP3 or control cDNA were injected into the tumors when their volume reached ~50 mm 3 ( n = 5). Serum VEGF levels of mice bearing control or FOXP3-overexpressing tumors were detected by ELISA. a – l Student’s t test
Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Transfection, shRNA, Western Blot, Confocal Microscopy, Over Expression, Injection, Multiple Displacement Amplification, Mouse Assay, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: FOXP3 is a transcriptional suppressor of VEGF. a FOXP3 suppresses VEGF promoter activity, as evaluated by dual-luciferase reporter assays. b The top panel depicts schematic diagrams of the regions amplified by the ChIP primers. The bottom panel shows the amount of DNA precipitated by either the anti-FOXP3 antibody or control IgG; the results are expressed as a percentage of the input genomic DNA from MDA-MB-231 cells. c FOXP3-mediated suppression of the VEGF promoter requires forkhead-binding motifs, as evaluated by dual-luciferase reporter assays. Relative truncation of the VEGF promoter is illustrated in the left panel. Luciferase activity was detected in cells transfected with the truncated VEGF promoter in the right panel. a , c ANOVA with Tukey’s post hoc test
Techniques Used: Activity Assay, Luciferase, Amplification, Chromatin Immunoprecipitation, Multiple Displacement Amplification, Binding Assay, Transfection
18) Product Images from "Early expressions of hypoxia-inducible factor 1alpha and vascular endothelial growth factor increase the neuronal plasticity of activated endogenous neural stem cells after focal cerebral ischemia"
Article Title: Early expressions of hypoxia-inducible factor 1alpha and vascular endothelial growth factor increase the neuronal plasticity of activated endogenous neural stem cells after focal cerebral ischemia
Journal: Neural Regeneration Research
doi: 10.4103/1673-5374.133136

Figure Legend Snippet: Western blot analysis for hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF) and nestin expression after focal cerebral ischemia. (A) Representative time course of nestin, HIF-1α, VEGF, and nestin expression in control (sham) and ipsilateral cerebral hemisphere extracts from rats at baseline, 1 h, 12 h, 1 d, 3 d, and 7 d. (B) Time courses of normalized optical density of nestin immunoblots in ipsilateral hemispheres (* P
Techniques Used: Western Blot, Expressing
19) Product Images from "Promotive effects of bone morphogenetic protein 2 on angiogenesis in hepatocarcinoma via multiple signal pathways"
Article Title: Promotive effects of bone morphogenetic protein 2 on angiogenesis in hepatocarcinoma via multiple signal pathways
Journal: Scientific Reports
doi: 10.1038/srep37499

Figure Legend Snippet: Expressions of VEGF, p-P38, p-ERK, p-AKT and p-m-TOR detected by Western blot. ( A ) BMP2 silence down-regulates the expression of VEGF, p-P38, p-ERK, p-AKT and p-m-TOR expressions ( B ) The protein expressions of VEGF, p-P38, p-ERK, p-AKT and p-m-TOR are up-regulated in overexpression-LV-BMP2 group Notes: BMP-2: Bone morphogenetic protein 2; VEGF: vascular endothelial growth factor * Indicates P
Techniques Used: Western Blot, Expressing, Over Expression
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