vegf protein  (R&D Systems)

 
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    Name:
    Recombinant Canine VEGF Protein CF
    Description:
    The Recombinant Canine VEGF Protein from R D Systems is derived from E coli The Recombinant Canine VEGF Protein has been validated for the following applications Bioactivity
    Catalog Number:
    1603-cv-010/cf
    Price:
    329
    Category:
    Proteins and Enzymes
    Source:
    E. coli-derived Recombinant Canine VEGF Protein
    Applications:
    Bioactivity
    Purity:
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
    Conjugate:
    Unconjugated
    Size:
    10 ug
    Buy from Supplier


    Structured Review

    R&D Systems vegf protein
    ZEB2 modulates expression of Sp1-regulated genes ( A ) SW480 cells were transfected with a ZEB2 expression vector for 48 h. Transfected cells were lysed and used for immunoblotting. An anti-myc antibody was used to detect myc-tagged ZEB2. GAPDH or β-actin was used as an internal control. ( B – D ) SNU-398 cells were transfected with ZEB2-specific siRNA or scrambled siRNA for 48 h. (B) Transfected cells were lysed for immunoblot analysis. Densitometry quantification was performed on the immunoblots, using GAPDH as a loading control. (C) Conditioned medium from transfected cells was collected for an additional 48 h. <t>VEGF</t> levels in conditioned medium were determined by an <t>ELISA.</t> (D) Real-time qPCR analysis of ZEB2, VEGF, cyclin D1, and survivin mRNA levels. ( E ) SNU-398 cells were co-transfected with ZEB2-specific siRNA and a VEGF promoter (−2361/+298) reporter construct. Firefly luciferase activity representing VEGF promoter activity was measured after 48 h and normalized to the fluorescence signal intensity to measure the transfection efficiency. Values represent mean standard deviation. * P
    The Recombinant Canine VEGF Protein from R D Systems is derived from E coli The Recombinant Canine VEGF Protein has been validated for the following applications Bioactivity
    https://www.bioz.com/result/vegf protein/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf protein - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF"

    Article Title: Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF

    Journal: Oncotarget

    doi: 10.18632/oncotarget.23139

    ZEB2 modulates expression of Sp1-regulated genes ( A ) SW480 cells were transfected with a ZEB2 expression vector for 48 h. Transfected cells were lysed and used for immunoblotting. An anti-myc antibody was used to detect myc-tagged ZEB2. GAPDH or β-actin was used as an internal control. ( B – D ) SNU-398 cells were transfected with ZEB2-specific siRNA or scrambled siRNA for 48 h. (B) Transfected cells were lysed for immunoblot analysis. Densitometry quantification was performed on the immunoblots, using GAPDH as a loading control. (C) Conditioned medium from transfected cells was collected for an additional 48 h. VEGF levels in conditioned medium were determined by an ELISA. (D) Real-time qPCR analysis of ZEB2, VEGF, cyclin D1, and survivin mRNA levels. ( E ) SNU-398 cells were co-transfected with ZEB2-specific siRNA and a VEGF promoter (−2361/+298) reporter construct. Firefly luciferase activity representing VEGF promoter activity was measured after 48 h and normalized to the fluorescence signal intensity to measure the transfection efficiency. Values represent mean standard deviation. * P
    Figure Legend Snippet: ZEB2 modulates expression of Sp1-regulated genes ( A ) SW480 cells were transfected with a ZEB2 expression vector for 48 h. Transfected cells were lysed and used for immunoblotting. An anti-myc antibody was used to detect myc-tagged ZEB2. GAPDH or β-actin was used as an internal control. ( B – D ) SNU-398 cells were transfected with ZEB2-specific siRNA or scrambled siRNA for 48 h. (B) Transfected cells were lysed for immunoblot analysis. Densitometry quantification was performed on the immunoblots, using GAPDH as a loading control. (C) Conditioned medium from transfected cells was collected for an additional 48 h. VEGF levels in conditioned medium were determined by an ELISA. (D) Real-time qPCR analysis of ZEB2, VEGF, cyclin D1, and survivin mRNA levels. ( E ) SNU-398 cells were co-transfected with ZEB2-specific siRNA and a VEGF promoter (−2361/+298) reporter construct. Firefly luciferase activity representing VEGF promoter activity was measured after 48 h and normalized to the fluorescence signal intensity to measure the transfection efficiency. Values represent mean standard deviation. * P

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Western Blot, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Construct, Luciferase, Activity Assay, Fluorescence, Standard Deviation

    2) Product Images from "Cardiac glycosides suppress the maintenance of stemness and malignancy via inhibiting HIF-1α in human glioma stem cells"

    Article Title: Cardiac glycosides suppress the maintenance of stemness and malignancy via inhibiting HIF-1α in human glioma stem cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.16714

    DT inhibits GSC angiogenic and invasive capacities ( A ) GSC were treated with various concentrations of DT (1–50 nM) for 16 h during hypoxia. Concentration of VEGF protein in the culture medium was determined by ELISA. The assays were performed in triplicates. ( B ) GSC were treated with DMSO or DT, and were cultured in sphere forming conditions in the upper well of matrigel-precoated transwell chambers for 16 h. Cells invading the lower well were fixed and stained with a Diff-Quick kit and photographed (100× magnification). Invasiveness was determined by counting cells in five randomly selected microscopic fields per well. ( C and D ) Western blot analysis shows that DT downregulates expression of invasion-related proteins in GSC during hypoxic conditions. Error Bars represent mean ± SD of triplicate samples. *P
    Figure Legend Snippet: DT inhibits GSC angiogenic and invasive capacities ( A ) GSC were treated with various concentrations of DT (1–50 nM) for 16 h during hypoxia. Concentration of VEGF protein in the culture medium was determined by ELISA. The assays were performed in triplicates. ( B ) GSC were treated with DMSO or DT, and were cultured in sphere forming conditions in the upper well of matrigel-precoated transwell chambers for 16 h. Cells invading the lower well were fixed and stained with a Diff-Quick kit and photographed (100× magnification). Invasiveness was determined by counting cells in five randomly selected microscopic fields per well. ( C and D ) Western blot analysis shows that DT downregulates expression of invasion-related proteins in GSC during hypoxic conditions. Error Bars represent mean ± SD of triplicate samples. *P

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Staining, Diff-Quik, Western Blot, Expressing

    3) Product Images from "Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases"

    Article Title: Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases

    Journal: Mediators of Inflammation

    doi: 10.1155/2015/438085

    VEGF protein expression . (a) VEGF protein expression in PDL cells was measured using ELISA. Supernatants of PDL cells, stimulated with or without LPS-PG (1 μ g/mL) under normoxic or hypoxic condition, were collected after varying time points (2, 4, 8, 24, and 48 h). Statistical differences were analyzed by one-way ANOVA and the post hoc Dunnett's and Tukey's multiple comparison test; # P
    Figure Legend Snippet: VEGF protein expression . (a) VEGF protein expression in PDL cells was measured using ELISA. Supernatants of PDL cells, stimulated with or without LPS-PG (1 μ g/mL) under normoxic or hypoxic condition, were collected after varying time points (2, 4, 8, 24, and 48 h). Statistical differences were analyzed by one-way ANOVA and the post hoc Dunnett's and Tukey's multiple comparison test; # P

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Collateral Expression of Proangiogenic and Tumorigenic Properties in Intestinal Epithelial Cell Variants Selected for Resistance to Anoikis"

    Article Title: Collateral Expression of Proangiogenic and Tumorigenic Properties in Intestinal Epithelial Cell Variants Selected for Resistance to Anoikis

    Journal: Neoplasia (New York, N.Y.)

    doi:

    Upregulation of VEGF mRNA expression in anoikis-resistant cell lines selected in spheroid culture. IEC-18—parental cells; AR1.10 and AR2.10—spontaneously derived anoikis-resistant variants of IEC-18; ras-4—IEC-18 cells transfected with mutant H-ras.
    Figure Legend Snippet: Upregulation of VEGF mRNA expression in anoikis-resistant cell lines selected in spheroid culture. IEC-18—parental cells; AR1.10 and AR2.10—spontaneously derived anoikis-resistant variants of IEC-18; ras-4—IEC-18 cells transfected with mutant H-ras.

    Techniques Used: Expressing, Derivative Assay, Transfection, Mutagenesis

    5) Product Images from "Stretch-Induced Hypertrophy Activates NFkB-Mediated VEGF Secretion in Adult Cardiomyocytes"

    Article Title: Stretch-Induced Hypertrophy Activates NFkB-Mediated VEGF Secretion in Adult Cardiomyocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0029055

    Cyclic mechanical stretch-induced VEGF secretion is independent of the MAPK/ERK1/2 or PI3K pathways. Inhibition of either the MAPK/ERK1/2 or PI3K signaling pathways in isolated ARCMs did not block VEGF secretion induced by cyclic mechanical stretch. Isolated ARCMs attached to laminin for 24 h were treated with the ERK1/2 kinase inhibitor (U0126; 0.5 µM) or DMSO-carrier control and subjected to ( A ) 24 h or 48 h of cyclic mechanical stretch (10% stretch) or no stretch (0% stretch). ELISA was used to determine the amount of VEGF secreted into the media. ( B ) The ERK1/2 kinase inhibitor was active in isolated primary ARCMs as pERK1/2 levels decreased in cells treated with the ERK1/2 kinase inhibitor (U0126; 0.5 µM) compared to the DMSO-carrier control. Stretch-activated VEGF secretion is independent of PI3K. ARCMs attached to laminin for 24 h were treated with the PI3K inhibitor (LY294002; 0.5 µM) or DMSO-carrier control and subjected to 24 h or 48 h ( C ) of cyclic mechanical stretch (10% stretch) or no stretch (0% stretch). ELISA was used to determine the amount of VEGF secreted into the media. ( D ) The PI3K inhibitor (LY294002; 0.5 µM) was active in isolated ARCMs as pAKT levels decreased in cells treated with the PI3K inhibitor (LY294002; 0.5 µM) compared to the DMSO-carrier control. Relative intensity of phospho-ERK1/2/total ERK1/2 (pERK/tERK) or phospho-AKT/total AKT (pAKT/tAKT) was determined. ( E ) ELISA was used to determine the amount of BNP secreted into the media. Treatment with the either ERK1/2 (U0126) or PI3K (LY294002) inhibitors blocked BNP secretion at 24 hours compared to DMSO-carrier control. *** P
    Figure Legend Snippet: Cyclic mechanical stretch-induced VEGF secretion is independent of the MAPK/ERK1/2 or PI3K pathways. Inhibition of either the MAPK/ERK1/2 or PI3K signaling pathways in isolated ARCMs did not block VEGF secretion induced by cyclic mechanical stretch. Isolated ARCMs attached to laminin for 24 h were treated with the ERK1/2 kinase inhibitor (U0126; 0.5 µM) or DMSO-carrier control and subjected to ( A ) 24 h or 48 h of cyclic mechanical stretch (10% stretch) or no stretch (0% stretch). ELISA was used to determine the amount of VEGF secreted into the media. ( B ) The ERK1/2 kinase inhibitor was active in isolated primary ARCMs as pERK1/2 levels decreased in cells treated with the ERK1/2 kinase inhibitor (U0126; 0.5 µM) compared to the DMSO-carrier control. Stretch-activated VEGF secretion is independent of PI3K. ARCMs attached to laminin for 24 h were treated with the PI3K inhibitor (LY294002; 0.5 µM) or DMSO-carrier control and subjected to 24 h or 48 h ( C ) of cyclic mechanical stretch (10% stretch) or no stretch (0% stretch). ELISA was used to determine the amount of VEGF secreted into the media. ( D ) The PI3K inhibitor (LY294002; 0.5 µM) was active in isolated ARCMs as pAKT levels decreased in cells treated with the PI3K inhibitor (LY294002; 0.5 µM) compared to the DMSO-carrier control. Relative intensity of phospho-ERK1/2/total ERK1/2 (pERK/tERK) or phospho-AKT/total AKT (pAKT/tAKT) was determined. ( E ) ELISA was used to determine the amount of BNP secreted into the media. Treatment with the either ERK1/2 (U0126) or PI3K (LY294002) inhibitors blocked BNP secretion at 24 hours compared to DMSO-carrier control. *** P

    Techniques Used: Inhibition, Isolation, Blocking Assay, Enzyme-linked Immunosorbent Assay

    Inhibition of the NFkB pathway blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. Primary ARCMs cultured on laminin for 24 h were incubated with the NFkB inhibitor (SN50; 15 µg/ml) or its inactive control peptide (SN50M; 15 µg/ml), and subjected to 24 h or 48 h of cyclic mechanical stretch (10% stretch). Non-stretched control cells (0% stretch) were incubated under identical conditions. ELISA was used to analyze VEGF levels secreted into the culture media at 24 h ( A ) or 48 h ( B ). Inhibition of the NFkB pathway blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. Isolated primary ARCMs cultured on laminin were treated with NFkB inhibitor (SN50; 15 µg/ml or 30 µg/ml) and stretched (10% stretch) for 24 h ( C ) or 48 h ( D ). ELISA was used to analyze the concentration of VEGF in the media. Isolated primary ARCMs treated with the NFkB inhibitor (SN50) or its inactive peptide control (SN50M) remained viable at 24 h ( E ) and 48 h ( F ) of culture as assessed by the MTT cell viability assay. Fold induction in relative mitochondrial activity represents the number of viable cells present after each treatment. ( G ) The NFkB peptide inhibitor (SN50) was active in isolated ARCMS as p65 levels were reduced in the nuclear fraction upon inhibitor treatment compared to peptide control. *** P
    Figure Legend Snippet: Inhibition of the NFkB pathway blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. Primary ARCMs cultured on laminin for 24 h were incubated with the NFkB inhibitor (SN50; 15 µg/ml) or its inactive control peptide (SN50M; 15 µg/ml), and subjected to 24 h or 48 h of cyclic mechanical stretch (10% stretch). Non-stretched control cells (0% stretch) were incubated under identical conditions. ELISA was used to analyze VEGF levels secreted into the culture media at 24 h ( A ) or 48 h ( B ). Inhibition of the NFkB pathway blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. Isolated primary ARCMs cultured on laminin were treated with NFkB inhibitor (SN50; 15 µg/ml or 30 µg/ml) and stretched (10% stretch) for 24 h ( C ) or 48 h ( D ). ELISA was used to analyze the concentration of VEGF in the media. Isolated primary ARCMs treated with the NFkB inhibitor (SN50) or its inactive peptide control (SN50M) remained viable at 24 h ( E ) and 48 h ( F ) of culture as assessed by the MTT cell viability assay. Fold induction in relative mitochondrial activity represents the number of viable cells present after each treatment. ( G ) The NFkB peptide inhibitor (SN50) was active in isolated ARCMS as p65 levels were reduced in the nuclear fraction upon inhibitor treatment compared to peptide control. *** P

    Techniques Used: Inhibition, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Isolation, Concentration Assay, MTT Assay, Viability Assay, Activity Assay

    Expression of a dominant negative mutant IkBα blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. Primary ARCMs transduced with a recombinant adenovirus encoding an IkBα dominant negative mutant (DN IkBα) were cultured on laminin for 24 h and then subjected to 24 h of cyclic mechanical stretch (10% stretch). Non-stretched control cells (0% stretch) were incubated under identical conditions. ELISA was used to determine VEGF levels secreted into the culture media at 24 h ( A ). ( B ) Expression of the IkBα dominant negative mutant blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. ELISA was used to analyze the concentration of VEGF in the media. ( C ) The IkBα dominant negative mutant was expressed in isolated ARCMs as IkBα levels were significantly increased in ARCMs expressing the mutant compared to control ARCMs. ( D ) The IkBα dominant negative mutant was active in isolated ARCMs as p65 levels were significantly decreased in the nucleus of ARCMs expressing the mutant compared to control ARCMs. *** P
    Figure Legend Snippet: Expression of a dominant negative mutant IkBα blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. Primary ARCMs transduced with a recombinant adenovirus encoding an IkBα dominant negative mutant (DN IkBα) were cultured on laminin for 24 h and then subjected to 24 h of cyclic mechanical stretch (10% stretch). Non-stretched control cells (0% stretch) were incubated under identical conditions. ELISA was used to determine VEGF levels secreted into the culture media at 24 h ( A ). ( B ) Expression of the IkBα dominant negative mutant blocks cyclic mechanical stretch-induced VEGF secretion in a dose-dependent manner. ELISA was used to analyze the concentration of VEGF in the media. ( C ) The IkBα dominant negative mutant was expressed in isolated ARCMs as IkBα levels were significantly increased in ARCMs expressing the mutant compared to control ARCMs. ( D ) The IkBα dominant negative mutant was active in isolated ARCMs as p65 levels were significantly decreased in the nucleus of ARCMs expressing the mutant compared to control ARCMs. *** P

    Techniques Used: Expressing, Dominant Negative Mutation, Transduction, Recombinant, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Concentration Assay, Isolation, Mutagenesis

    Cyclic mechanical stretch induces VEGF secretion in primary ARCMs. A ) Cyclic mechanical stretch for either 24 h or 48 h induces a significant increase in VEGF secretion in primary ARCMs attached to laminin compared to non-stretched controls. Stretched (10% stretch) and non-stretched control (0% stretch) cells were allowed to adhere to laminin for 24 h prior to initiating experiments. VEGF concentration in the conditioned media of non-stretched and stretched ARCMs was analyzed by ELISA. *** P
    Figure Legend Snippet: Cyclic mechanical stretch induces VEGF secretion in primary ARCMs. A ) Cyclic mechanical stretch for either 24 h or 48 h induces a significant increase in VEGF secretion in primary ARCMs attached to laminin compared to non-stretched controls. Stretched (10% stretch) and non-stretched control (0% stretch) cells were allowed to adhere to laminin for 24 h prior to initiating experiments. VEGF concentration in the conditioned media of non-stretched and stretched ARCMs was analyzed by ELISA. *** P

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay

    6) Product Images from "Disruption of Gap Junctions Reduces Biomarkers of Decidualization and Angiogenesis and Increases Inflammatory Mediators in Human Endometrial Stromal Cell Cultures"

    Article Title: Disruption of Gap Junctions Reduces Biomarkers of Decidualization and Angiogenesis and Increases Inflammatory Mediators in Human Endometrial Stromal Cell Cultures

    Journal: Molecular and cellular endocrinology

    doi: 10.1016/j.mce.2011.04.011

    Inhibitory effects of AGA on VEGF production are partly mediated at the level of mRNA metabolism. Real-time RT-PCR was used to quantify VEGF mRNA transcripts in decidualized ESC. Incubation with E/P/c for 8 days in the presence of AGA resulted in ~50%
    Figure Legend Snippet: Inhibitory effects of AGA on VEGF production are partly mediated at the level of mRNA metabolism. Real-time RT-PCR was used to quantify VEGF mRNA transcripts in decidualized ESC. Incubation with E/P/c for 8 days in the presence of AGA resulted in ~50%

    Techniques Used: Quantitative RT-PCR, Incubation

    Time course of biochemical differentiation of ESC exposed to E/P/c. (A) Prolactin and (B) VEGF secretion both increased following hormone stimulation in vitro . Grey histograms indicate protein secretion after 4 d incubation without (control) and with
    Figure Legend Snippet: Time course of biochemical differentiation of ESC exposed to E/P/c. (A) Prolactin and (B) VEGF secretion both increased following hormone stimulation in vitro . Grey histograms indicate protein secretion after 4 d incubation without (control) and with

    Techniques Used: In Vitro, Incubation

    Gap junction inhibitors block biochemical markers of angiogenesis in decidualized ESC. VEGF secretion as detected by ELISA and expressed as pg/10 6 cells, was significantly upregulated in cells cultured for 8 days with E/P/c relative to cells without hormone
    Figure Legend Snippet: Gap junction inhibitors block biochemical markers of angiogenesis in decidualized ESC. VEGF secretion as detected by ELISA and expressed as pg/10 6 cells, was significantly upregulated in cells cultured for 8 days with E/P/c relative to cells without hormone

    Techniques Used: Blocking Assay, Enzyme-linked Immunosorbent Assay, Cell Culture

    7) Product Images from "Targeting Notch1 inhibits invasion and angiogenesis of human breast cancer cells via inhibition Nuclear Factor-κB signaling"

    Article Title: Targeting Notch1 inhibits invasion and angiogenesis of human breast cancer cells via inhibition Nuclear Factor-κB signaling

    Journal: American Journal of Translational Research

    doi:

    Notch-1 overexpression on NF-κB DNA-binding activity and its target genes. The SKBR-3 cells were transfected with human Notch-1 cDNA for 36 hs.The NF-κB DNA-binding activity was detedted by EMSA (A). The expression of IL-8, MMP-9 and VEGF was detected by western blot assay (B) and RT-PCR assay (C). The culture supernatant of IL-8, MMP-9 and VEGF was detected by ELISA (D-F). Statistical significance of three or more experiments was determined by performing a two-tailed, unpaired Student’s t -test for two comparisons. vs control, * p
    Figure Legend Snippet: Notch-1 overexpression on NF-κB DNA-binding activity and its target genes. The SKBR-3 cells were transfected with human Notch-1 cDNA for 36 hs.The NF-κB DNA-binding activity was detedted by EMSA (A). The expression of IL-8, MMP-9 and VEGF was detected by western blot assay (B) and RT-PCR assay (C). The culture supernatant of IL-8, MMP-9 and VEGF was detected by ELISA (D-F). Statistical significance of three or more experiments was determined by performing a two-tailed, unpaired Student’s t -test for two comparisons. vs control, * p

    Techniques Used: Over Expression, Binding Assay, Activity Assay, Transfection, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    Down-regulation of Notch-1 on NF-κB DNA-binding activity and its target genes. The MDA-MB-231 cells were transfected with human Notch-1 siRNA or control siRNA for 48 hs. The expression of IL-8, MMP-9 and VEGF was detected by western blot assay (A) and RT-PCR assay (B). NF-κB DNA-binding activity was detedted by EMSA (C). The culture supernatant of IL-8, MMP-9 and VEGF was detected by ELISA (D-F). Statistical significance of three or more experiments was determined by performing a two-tailed, unpaired Student’s t -test for two comparisons. vs control, * p
    Figure Legend Snippet: Down-regulation of Notch-1 on NF-κB DNA-binding activity and its target genes. The MDA-MB-231 cells were transfected with human Notch-1 siRNA or control siRNA for 48 hs. The expression of IL-8, MMP-9 and VEGF was detected by western blot assay (A) and RT-PCR assay (B). NF-κB DNA-binding activity was detedted by EMSA (C). The culture supernatant of IL-8, MMP-9 and VEGF was detected by ELISA (D-F). Statistical significance of three or more experiments was determined by performing a two-tailed, unpaired Student’s t -test for two comparisons. vs control, * p

    Techniques Used: Binding Assay, Activity Assay, Multiple Displacement Amplification, Transfection, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    8) Product Images from "Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in M?ller Cells"

    Article Title: Melatonin-Mediated Cytoprotection against Hyperglycemic Injury in M?ller Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050661

    Melatonin-mediated inhibition of VEGF production from Müller cells. (A) Cells were treated with either 30 mM glucose (HG) or glucose and 100 µM melatonin (HG+Mel) for indicated time. The amount of VEGF secreted in the conditioned media was measured by ELISA. (B) Dose-dependent inhibition of VEGF production by melatonin. VEGF production was measured at 48 hr after high glucose stimulation in the presence of different concentrations of melatonin. Data presented are the average of 2 separate experiments. *P
    Figure Legend Snippet: Melatonin-mediated inhibition of VEGF production from Müller cells. (A) Cells were treated with either 30 mM glucose (HG) or glucose and 100 µM melatonin (HG+Mel) for indicated time. The amount of VEGF secreted in the conditioned media was measured by ELISA. (B) Dose-dependent inhibition of VEGF production by melatonin. VEGF production was measured at 48 hr after high glucose stimulation in the presence of different concentrations of melatonin. Data presented are the average of 2 separate experiments. *P

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay

    9) Product Images from "The enhancement of radiosensitivity in human esophageal squamous cell carcinoma cells by zoledronic acid and its potential mechanism"

    Article Title: The enhancement of radiosensitivity in human esophageal squamous cell carcinoma cells by zoledronic acid and its potential mechanism

    Journal: Cytotechnology

    doi: 10.1007/s10616-012-9532-4

    Suppression of VEGF expression by combined treatment of ZOL + IR. EC109 cells were treated with ZOL (8 μM), IR (4 Gy), or ZOL + IR and then the expression of secreted VEGF protein was detected by Western blot ( left ) and ELISA ( right ) analyses. Quantified data are expressed as mean ± SD. Difference between either ZOL or IR alone and combination of ZOL + IR was significant ( ★ P
    Figure Legend Snippet: Suppression of VEGF expression by combined treatment of ZOL + IR. EC109 cells were treated with ZOL (8 μM), IR (4 Gy), or ZOL + IR and then the expression of secreted VEGF protein was detected by Western blot ( left ) and ELISA ( right ) analyses. Quantified data are expressed as mean ± SD. Difference between either ZOL or IR alone and combination of ZOL + IR was significant ( ★ P

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery
    Article Snippet: .. The amount of VEGF protein secreted by ARPE-19 into the culture medium was measured by enzyme-linked immunosorbent assay (R & D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. The supernatants were diluted if necessary and analyzed for VEGF content by measuring the intensity of fluorescence using an enzyme-linked immunosorbent assay reader (Infinite® 200 PRO, Tecan Group Ltd., Männedorf, Switzerland) at 450 nm against the standard curve.

    Article Title: Proteinuria Triggers Renal Lymphangiogenesis Prior to the Development of Interstitial Fibrosis
    Article Snippet: .. VEGF-C ELISA Measurement The level of VEGF-C protein secreted into cell culture supernatant was measured by Human VEGF-C Immunoassay kit (R & D Systems, Wiesbaden-Nordernstadt, Germany), according to manufacturer’s instructions. ..

    Article Title: Skeletal myofiber VEGF regulates contraction-induced perfusion and exercise capacity but not muscle capillarity in adult mice
    Article Snippet: Additional groups of mice were used for ex vivo and in situ muscle stimulation protocols. .. VEGF protein levels were measured using an ELISA kit for mouse (VEGF mouse ELISA, R & D Systems, La Jolla, CA) and were normalized to total protein measured by Bio-Rad DC protein assay. .. All mice were familiarized on a treadmill (model no. CL-4, Omnitech, Columbus, OH) for 10 min on each of several days prior to performing the exercise tests.

    Article Title: Doxycycline modulates VEGF-A expression: Failure of doxycycline-inducible lentivirus shRNA vector to knockdown VEGF-A expression in transgenic mice
    Article Snippet: The cell pellet was lysed with western blot lysis buffer (50 mM Tris-Hcl, ph 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.5% Na-deoxycholate, 0.1% SDS, 10% glycerol) with proteinase inhibitor cocktail (Halt proteinase inhibitor cocktail kit, Thermo Fisher Scientific, Waltham, MA, USA) and stored at -20°C. .. VEGF-A protein levels were determined with ELISA (mouse VEGF-A ELISA kit, R & D Systems, Minneapolis, MN, USA) and protein concentrations were measured with BCA Protein Assay Kit (Pierce, Rockford, IL, USA). .. The effect of T-1 VEGF-A knockdown vector was also studied in HL-1 cardiomyocyte cell line cultured in Claycomb medium (Sigma-Aldrich, St Louis, MO, USA) supplied with 10% FBS– 100 μg/ml penicillin-streptomycin– 0.1 mM norepinephrine– 2 mM L-glutamine (Sigma-Aldrich, St Louis, MO, USA) with viral doses of MOI 1 and 2 and dox induction times of 3–4 days as described above.

    Article Title: Virotherapy of Canine Tumors with Oncolytic Vaccinia Virus GLV-1h109 Expressing an Anti-VEGF Single-Chain Antibody
    Article Snippet: Therefore we first analyzed the VEGF expression of the two tested canine cancer cell lines under cell culture conditions ( ). .. Canine VEGF concentrations were determined using a Quantikine ELISA kit (R & D Systems, Minneapolis, MN, USA) developed for detection of canine VEGF, in accordance with the manufacturer’s directions. .. Concentration of VEGF in supernatant was represented as pg/106 cells.

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    Article Snippet: .. Plasma EPO and VEGF protein were determined by enzyme-linked immunosorbent assay (ELISA; R & D systems kits MEP00, EPO; and MMV00, VEGF). .. The VEGF ELISA recognizes both the 164 and 120 amino acid residue forms of mouse VEGF.

    Cell Culture:

    Article Title: Proteinuria Triggers Renal Lymphangiogenesis Prior to the Development of Interstitial Fibrosis
    Article Snippet: .. VEGF-C ELISA Measurement The level of VEGF-C protein secreted into cell culture supernatant was measured by Human VEGF-C Immunoassay kit (R & D Systems, Wiesbaden-Nordernstadt, Germany), according to manufacturer’s instructions. ..

    DC Protein Assay:

    Article Title: Skeletal myofiber VEGF regulates contraction-induced perfusion and exercise capacity but not muscle capillarity in adult mice
    Article Snippet: Additional groups of mice were used for ex vivo and in situ muscle stimulation protocols. .. VEGF protein levels were measured using an ELISA kit for mouse (VEGF mouse ELISA, R & D Systems, La Jolla, CA) and were normalized to total protein measured by Bio-Rad DC protein assay. .. All mice were familiarized on a treadmill (model no. CL-4, Omnitech, Columbus, OH) for 10 min on each of several days prior to performing the exercise tests.

    BIA-KA:

    Article Title: Doxycycline modulates VEGF-A expression: Failure of doxycycline-inducible lentivirus shRNA vector to knockdown VEGF-A expression in transgenic mice
    Article Snippet: The cell pellet was lysed with western blot lysis buffer (50 mM Tris-Hcl, ph 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.5% Na-deoxycholate, 0.1% SDS, 10% glycerol) with proteinase inhibitor cocktail (Halt proteinase inhibitor cocktail kit, Thermo Fisher Scientific, Waltham, MA, USA) and stored at -20°C. .. VEGF-A protein levels were determined with ELISA (mouse VEGF-A ELISA kit, R & D Systems, Minneapolis, MN, USA) and protein concentrations were measured with BCA Protein Assay Kit (Pierce, Rockford, IL, USA). .. The effect of T-1 VEGF-A knockdown vector was also studied in HL-1 cardiomyocyte cell line cultured in Claycomb medium (Sigma-Aldrich, St Louis, MO, USA) supplied with 10% FBS– 100 μg/ml penicillin-streptomycin– 0.1 mM norepinephrine– 2 mM L-glutamine (Sigma-Aldrich, St Louis, MO, USA) with viral doses of MOI 1 and 2 and dox induction times of 3–4 days as described above.

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  • 93
    R&D Systems vegf sema3f recombinant protein
    <t>Sema3F</t> inhibits angiogenic sprouting of both retinal and choroidal endothelial cells. (A) Human retinal endothelial cells (HRECs) cultivated as multicellular spheroids sprout into the surrounding collagen matrix when stimulated with <t>VEGF</t> (25 ng/ml; set
    Vegf Sema3f Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems vegf a 165
    MMP-1-PAR1 stimulates secretion of CXCR1/2 chemokines from ovarian carcinoma cells. A , OVCAR-4 cells were stimulated with either 1 nM MMP-1 or PBS buffer in RPMI with 0.1% BSA and conditioned media (CM) was collected after 18 h. The angiogenesis array membranes were incubated with CM from the OVCAR-4 cells and fold change of the spot intensities was quantified using NIH ImageJ software. B ) in OVCAR-4, IGROV-1, OVCAR-3 and OVCAR-4/PAR1 shRNAi ovarian carcinoma cell lines. C–E, ELISA analysis was used to measure IL-8 (C), GRO-α (D), and <t>VEGF-A-165</t> (E) levels in CM from various ovarian carcinoma cell lines that were stimulated with 1 nM MMP-1 in the presence or absence of the PAR1 antagonist pepducin P1pal-7 (3 μM), the small molecule PAR1 antagonist RWJ-56110 (5 μM), or buffer control as indicated. Data (mean ± SE) are from 2–4 experiments performed in duplicate.
    Vegf A 165, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems vegf165
    Human ADSC viability and VEGF expression after transplantation to ischemic murine muscle. A . Representative image of m. tibialis anterior section from “GFP-ADSC” group obtained at day 7 after ischemia induction and GFP-ADSC injection, 50× magnification. GFP-positive cells are distributed in tissue around injection site. B. Analysis of <t>VEGF165</t> content by ELISA in explants culture medium from “ADSC”, “GFP-ADSC”, “VEGF-ADSC” groups obtained at days 3 and 20 after cell trasplantation.
    Vegf165, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems human recombinant vegf c
    NIR fluorescent images of <t>VEGF-C</t> (n = 11) or PBS (n = 5) treated mice prior to and 2, 6, and 10 days after surgical removal of the left ALN. Double arrow, ICG injection site. Arrow head, ILN. Open arrow, internodal collecting lymphatic vessels. Asterisk, aberrant lymphatic drainage to the contralateral ALN. Double arrows, ICG injection site.
    Human Recombinant Vegf C, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Sema3F inhibits angiogenic sprouting of both retinal and choroidal endothelial cells. (A) Human retinal endothelial cells (HRECs) cultivated as multicellular spheroids sprout into the surrounding collagen matrix when stimulated with VEGF (25 ng/ml; set

    Journal: FEBS letters

    Article Title: Semaphorin 3F forms an anti-angiogenic barrier in outer retina

    doi: 10.1016/j.febslet.2013.04.008

    Figure Lengend Snippet: Sema3F inhibits angiogenic sprouting of both retinal and choroidal endothelial cells. (A) Human retinal endothelial cells (HRECs) cultivated as multicellular spheroids sprout into the surrounding collagen matrix when stimulated with VEGF (25 ng/ml; set

    Article Snippet: Freshly prepared gels were transferred rapidly into a humidified incubator (37 °C, 5% CO2 ), and after the gels had solidified, 0.1 ml of serum-free medium (Cell Systems) was added per well containing VEGF ± Sema3F recombinant protein (RnD Systems).

    Techniques:

    MMP-1-PAR1 stimulates secretion of CXCR1/2 chemokines from ovarian carcinoma cells. A , OVCAR-4 cells were stimulated with either 1 nM MMP-1 or PBS buffer in RPMI with 0.1% BSA and conditioned media (CM) was collected after 18 h. The angiogenesis array membranes were incubated with CM from the OVCAR-4 cells and fold change of the spot intensities was quantified using NIH ImageJ software. B ) in OVCAR-4, IGROV-1, OVCAR-3 and OVCAR-4/PAR1 shRNAi ovarian carcinoma cell lines. C–E, ELISA analysis was used to measure IL-8 (C), GRO-α (D), and VEGF-A-165 (E) levels in CM from various ovarian carcinoma cell lines that were stimulated with 1 nM MMP-1 in the presence or absence of the PAR1 antagonist pepducin P1pal-7 (3 μM), the small molecule PAR1 antagonist RWJ-56110 (5 μM), or buffer control as indicated. Data (mean ± SE) are from 2–4 experiments performed in duplicate.

    Journal: Cancer research

    Article Title: Identification of a metalloprotease-chemokine signaling system in the ovarian cancer microenvironment: implications for anti-angiogenic therapy

    doi: 10.1158/0008-5472.CAN-09-4341

    Figure Lengend Snippet: MMP-1-PAR1 stimulates secretion of CXCR1/2 chemokines from ovarian carcinoma cells. A , OVCAR-4 cells were stimulated with either 1 nM MMP-1 or PBS buffer in RPMI with 0.1% BSA and conditioned media (CM) was collected after 18 h. The angiogenesis array membranes were incubated with CM from the OVCAR-4 cells and fold change of the spot intensities was quantified using NIH ImageJ software. B ) in OVCAR-4, IGROV-1, OVCAR-3 and OVCAR-4/PAR1 shRNAi ovarian carcinoma cell lines. C–E, ELISA analysis was used to measure IL-8 (C), GRO-α (D), and VEGF-A-165 (E) levels in CM from various ovarian carcinoma cell lines that were stimulated with 1 nM MMP-1 in the presence or absence of the PAR1 antagonist pepducin P1pal-7 (3 μM), the small molecule PAR1 antagonist RWJ-56110 (5 μM), or buffer control as indicated. Data (mean ± SE) are from 2–4 experiments performed in duplicate.

    Article Snippet: Quantikine ELISAs for human IL-8, GRO-α and VEGF-A-165, were obtained from R & D Systems and used as recommended by the manufacturer.

    Techniques: Incubation, Software, Enzyme-linked Immunosorbent Assay

    Human ADSC viability and VEGF expression after transplantation to ischemic murine muscle. A . Representative image of m. tibialis anterior section from “GFP-ADSC” group obtained at day 7 after ischemia induction and GFP-ADSC injection, 50× magnification. GFP-positive cells are distributed in tissue around injection site. B. Analysis of VEGF165 content by ELISA in explants culture medium from “ADSC”, “GFP-ADSC”, “VEGF-ADSC” groups obtained at days 3 and 20 after cell trasplantation.

    Journal: Journal of Translational Medicine

    Article Title: Transplantation of modified human adipose derived stromal cells expressing VEGF165 results in more efficient angiogenic response in ischemic skeletal muscle

    doi: 10.1186/1479-5876-11-138

    Figure Lengend Snippet: Human ADSC viability and VEGF expression after transplantation to ischemic murine muscle. A . Representative image of m. tibialis anterior section from “GFP-ADSC” group obtained at day 7 after ischemia induction and GFP-ADSC injection, 50× magnification. GFP-positive cells are distributed in tissue around injection site. B. Analysis of VEGF165 content by ELISA in explants culture medium from “ADSC”, “GFP-ADSC”, “VEGF-ADSC” groups obtained at days 3 and 20 after cell trasplantation.

    Article Snippet: VEGF165 concentration in condition media samples was measured using human VEGF Quantikine Kit (R & D Systems, USA) following manufacturer’s protocol.

    Techniques: Expressing, Transplantation Assay, Injection, Enzyme-linked Immunosorbent Assay

    Validation of VEGF165 expression in AAV-modified VEGF-ADSC. A. VEGFA expression level in human ADSC 10 days after AAV transduction determined by quantitative PCR. B, C. Analysis of VEGF secretion by GFP-ADSC, VEGF-ADSC and unmodified cells using ELISA ( B ) and immunoblotting ( C ). In immunosorbent assay protein content was determined in conditioned media samples obtained at days 7 and 30 post genetic modification of ADSC.

    Journal: Journal of Translational Medicine

    Article Title: Transplantation of modified human adipose derived stromal cells expressing VEGF165 results in more efficient angiogenic response in ischemic skeletal muscle

    doi: 10.1186/1479-5876-11-138

    Figure Lengend Snippet: Validation of VEGF165 expression in AAV-modified VEGF-ADSC. A. VEGFA expression level in human ADSC 10 days after AAV transduction determined by quantitative PCR. B, C. Analysis of VEGF secretion by GFP-ADSC, VEGF-ADSC and unmodified cells using ELISA ( B ) and immunoblotting ( C ). In immunosorbent assay protein content was determined in conditioned media samples obtained at days 7 and 30 post genetic modification of ADSC.

    Article Snippet: VEGF165 concentration in condition media samples was measured using human VEGF Quantikine Kit (R & D Systems, USA) following manufacturer’s protocol.

    Techniques: Expressing, Modification, Transduction, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    NIR fluorescent images of VEGF-C (n = 11) or PBS (n = 5) treated mice prior to and 2, 6, and 10 days after surgical removal of the left ALN. Double arrow, ICG injection site. Arrow head, ILN. Open arrow, internodal collecting lymphatic vessels. Asterisk, aberrant lymphatic drainage to the contralateral ALN. Double arrows, ICG injection site.

    Journal: Biomedical Optics Express

    Article Title: Characterization of internodal collecting lymphatic vessel function after surgical removal of an axillary lymph node in mice

    doi: 10.1364/BOE.7.001100

    Figure Lengend Snippet: NIR fluorescent images of VEGF-C (n = 11) or PBS (n = 5) treated mice prior to and 2, 6, and 10 days after surgical removal of the left ALN. Double arrow, ICG injection site. Arrow head, ILN. Open arrow, internodal collecting lymphatic vessels. Asterisk, aberrant lymphatic drainage to the contralateral ALN. Double arrows, ICG injection site.

    Article Snippet: In separate groups of mice, mice were daily treated for 9 days, starting one day before axillary lymphadenectomy by i.d. injection into the wound with 200 ng of human recombinant VEGF-C (Cys156Ser; R & D Systems) dissolved in 10 µl PBS (n = 11).

    Techniques: Mouse Assay, Injection