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Bender MedSystems vegf c
<t>VEGF-C</t> and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,
Vegf C, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation"

Article Title: Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation

Journal: Journal of Clinical Investigation

doi: 10.1172/JCI200522037

VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,
Figure Legend Snippet: VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,

Techniques Used: Infection, Immunohistochemistry, Staining

2) Product Images from "MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis"

Article Title: MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis

Journal: Cancer Cell International

doi: 10.1186/1475-2867-13-98

The correlation between MT1-MMP and VEGF-C expression in human breast cancer cells. (a) MT1-MMP and VEGF-C mRNA expression. (b) MT1-MMP and VEGF-C protein expression.
Figure Legend Snippet: The correlation between MT1-MMP and VEGF-C expression in human breast cancer cells. (a) MT1-MMP and VEGF-C mRNA expression. (b) MT1-MMP and VEGF-C protein expression.

Techniques Used: Expressing

3) Product Images from "Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation"

Article Title: Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation

Journal: Journal of Clinical Investigation

doi: 10.1172/JCI200522037

VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,
Figure Legend Snippet: VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,

Techniques Used: Infection, Immunohistochemistry, Staining

4) Product Images from "Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis"

Article Title: Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis

Journal: Genes & Development

doi: 10.1101/gad.615311

Effects of AAV-VEGF-A and AAV-VEGF-C overexpression on the adult SVZ. Blood vessel pattern in the striatal wall, as shown by labeling of endothelial cells (CD31, green) and pericytes (PDGFR-β, red), while astrocytes are stained with anti-GFAP Ab (blue). AAV-VEGF-C-treated and AAV-VEGF-C156-treated (a VEGF-C variant that cannot bind to VEGFR-2) animals display a vascular network similar to AAV-ctl. In contrast, AAV-VEGF-A induced robust angiogenesis, which is attested to by a dense network of CD31 + endothelial cells and PDGFRβ + pericytes at the site of injection. Note that the number of astroglial cells increased in AAV-VEGF-C- and AAV-VEGF-C156-treated animals compared with controls. Bar, 20 μm.
Figure Legend Snippet: Effects of AAV-VEGF-A and AAV-VEGF-C overexpression on the adult SVZ. Blood vessel pattern in the striatal wall, as shown by labeling of endothelial cells (CD31, green) and pericytes (PDGFR-β, red), while astrocytes are stained with anti-GFAP Ab (blue). AAV-VEGF-C-treated and AAV-VEGF-C156-treated (a VEGF-C variant that cannot bind to VEGFR-2) animals display a vascular network similar to AAV-ctl. In contrast, AAV-VEGF-A induced robust angiogenesis, which is attested to by a dense network of CD31 + endothelial cells and PDGFRβ + pericytes at the site of injection. Note that the number of astroglial cells increased in AAV-VEGF-C- and AAV-VEGF-C156-treated animals compared with controls. Bar, 20 μm.

Techniques Used: Over Expression, Labeling, Staining, Variant Assay, CTL Assay, Injection

In vivo VEGF-C overexpression. ( A ) Adult mice are infected with either AAV-VEGF-C or control AAVs (AAV-ctl: AAV-EGFP and AAV-HSA) in the vicinity of the SVZ (coordinates from the bregma: anterior, 0.5; lateral, 1.0; depth, 2.1; n = 7 animals per group) and injected intraperitoneally with BrdU the same day. ( B ) Coronal brain cryosections from animals sacrificed at day 2 post-infection are analyzed for the number of BrdU cells (red) in the SVZ. Both the dorsolateral and ventral regions along the striatal wall show more BrdU + cells in AAV-VEGF-C-treated animals as compared with AAV-ctl. Histograms indicate the number of newborn BrdU + cells ( bottom left ) and activated Caspase-3 + cells ( bottom right ) in the SVZ. AAV-VEGF-C promotes the production of newborn cells and inhibits PCD in SVZ cells. ( C ) Coronal cryosections of the periventricular zone immunolabeled with anti-DCX Ab (green). In the striatal SVZ, DCX labeling reflects neuroblast production. AAV-VEGF-C induces a significant expansion of DCX expression in the SVZ compared with controls. ( D , E ) Coronal brain vibratome sections from Vegfr3:YFP adult mice infected with either AAV-VEGF-C or AAV-ctl and sacrificed at day 2. Dividing VEGFR-3-expressing cells (BrdU + YFP + ) are increased in AAV-VEGF-C-treated animals ( D ), while the subventricular expressions of GFAP and YFP are also enhanced ( E ), as compared with controls.(St) Striatum. Bars: B , C , 100 μm; D , E , 20 μm. Error bars indicate SEM. (***) P
Figure Legend Snippet: In vivo VEGF-C overexpression. ( A ) Adult mice are infected with either AAV-VEGF-C or control AAVs (AAV-ctl: AAV-EGFP and AAV-HSA) in the vicinity of the SVZ (coordinates from the bregma: anterior, 0.5; lateral, 1.0; depth, 2.1; n = 7 animals per group) and injected intraperitoneally with BrdU the same day. ( B ) Coronal brain cryosections from animals sacrificed at day 2 post-infection are analyzed for the number of BrdU cells (red) in the SVZ. Both the dorsolateral and ventral regions along the striatal wall show more BrdU + cells in AAV-VEGF-C-treated animals as compared with AAV-ctl. Histograms indicate the number of newborn BrdU + cells ( bottom left ) and activated Caspase-3 + cells ( bottom right ) in the SVZ. AAV-VEGF-C promotes the production of newborn cells and inhibits PCD in SVZ cells. ( C ) Coronal cryosections of the periventricular zone immunolabeled with anti-DCX Ab (green). In the striatal SVZ, DCX labeling reflects neuroblast production. AAV-VEGF-C induces a significant expansion of DCX expression in the SVZ compared with controls. ( D , E ) Coronal brain vibratome sections from Vegfr3:YFP adult mice infected with either AAV-VEGF-C or AAV-ctl and sacrificed at day 2. Dividing VEGFR-3-expressing cells (BrdU + YFP + ) are increased in AAV-VEGF-C-treated animals ( D ), while the subventricular expressions of GFAP and YFP are also enhanced ( E ), as compared with controls.(St) Striatum. Bars: B , C , 100 μm; D , E , 20 μm. Error bars indicate SEM. (***) P

Techniques Used: In Vivo, Over Expression, Mouse Assay, Infection, CTL Assay, Injection, Immunolabeling, Labeling, Expressing

Isolation of NSCs from adult Vegfr3:YFP mice. ( A ) YFP-positive and YFP-negative cells from the periventricular zone of Vegfr3:YFP mice were FACS-sorted. ( B ) The cells were tested for their ability to generate primary neurospheres in the presence of bFGF/EGF (2 × 10 4 cells per well in 24-well plates). Histograms show that YFP-positive and YFP-negative cells form primary neurospheres and include a subset of 0.5% of cell-forming spheres ( n = 6). ( C ) YFP-positive and YFP-negative cells from primary neurospheres are multipotent, differentiating into TuJ1 + neurons, GFAP + astrocytes, and O4 + oligodendrocytes after growth factor removal. Quantification is shown in the histogram ( n = 3). ( D ) YFP + /EGFR + , YFP + /EGFR − , YFP − /EGFR + , and YFP − /EGFR − subpopulations isolated after labeling with EGF-647 are tested for their capacity to self-renew and generate secondary (2 ary NS) and tertiary (3 ary NS) neurospheres. Histogram shows the increase in the number of neurosphere cells (NS-cells) relative to the number of plated cells. Only EGFR + /VEGFR-3 + cells generate numerous secondary neurospheres (2 ary NS) and tertiary neurospheres (3 ary NS) ( n = 4), even forming neurospheres after six passages (6 ary NS) (data not shown), and thus correspond to the majority of NSCs. ( E ) Primary neurospheres cultured in the presence of VEGF-C (50 ng/mL) and 31-C1 (a mouse VEGFR-3 function-blocking Ab, 5 μg/mL). The number of neurosphere formed (NS formed) is expressed as a percentage of the control. The 31-C1 Ab blocks the VEGF-C-induced amplification of neurospheres ( n = 3). ( F ) Dissociated neurosphere cells are counted and TUNEL-labeled in control or VEGF-C-containing (50 ng/mL) medium ( n = 3). ( G ) Increased proliferation of YFP + /EGFR + NSCs following VEGF-C treatment (50 ng/mL). Cells were pulsed with BrdU (10 μM) for 24 h and fixed after 2 d in vitro ( n = 3). Bar: C , 20 μm. Error bars indicate SEM. (*) P
Figure Legend Snippet: Isolation of NSCs from adult Vegfr3:YFP mice. ( A ) YFP-positive and YFP-negative cells from the periventricular zone of Vegfr3:YFP mice were FACS-sorted. ( B ) The cells were tested for their ability to generate primary neurospheres in the presence of bFGF/EGF (2 × 10 4 cells per well in 24-well plates). Histograms show that YFP-positive and YFP-negative cells form primary neurospheres and include a subset of 0.5% of cell-forming spheres ( n = 6). ( C ) YFP-positive and YFP-negative cells from primary neurospheres are multipotent, differentiating into TuJ1 + neurons, GFAP + astrocytes, and O4 + oligodendrocytes after growth factor removal. Quantification is shown in the histogram ( n = 3). ( D ) YFP + /EGFR + , YFP + /EGFR − , YFP − /EGFR + , and YFP − /EGFR − subpopulations isolated after labeling with EGF-647 are tested for their capacity to self-renew and generate secondary (2 ary NS) and tertiary (3 ary NS) neurospheres. Histogram shows the increase in the number of neurosphere cells (NS-cells) relative to the number of plated cells. Only EGFR + /VEGFR-3 + cells generate numerous secondary neurospheres (2 ary NS) and tertiary neurospheres (3 ary NS) ( n = 4), even forming neurospheres after six passages (6 ary NS) (data not shown), and thus correspond to the majority of NSCs. ( E ) Primary neurospheres cultured in the presence of VEGF-C (50 ng/mL) and 31-C1 (a mouse VEGFR-3 function-blocking Ab, 5 μg/mL). The number of neurosphere formed (NS formed) is expressed as a percentage of the control. The 31-C1 Ab blocks the VEGF-C-induced amplification of neurospheres ( n = 3). ( F ) Dissociated neurosphere cells are counted and TUNEL-labeled in control or VEGF-C-containing (50 ng/mL) medium ( n = 3). ( G ) Increased proliferation of YFP + /EGFR + NSCs following VEGF-C treatment (50 ng/mL). Cells were pulsed with BrdU (10 μM) for 24 h and fixed after 2 d in vitro ( n = 3). Bar: C , 20 μm. Error bars indicate SEM. (*) P

Techniques Used: Isolation, Mouse Assay, FACS, Labeling, Cell Culture, Blocking Assay, Amplification, TUNEL Assay, In Vitro

Proposed model for VEGF-C and VEGFR-3 function in the adult SVZ. VEGFR-3 is expressed by a subpopulation of astrocytes (B) and almost all NSCs (B1), but not by the majority of TAPs (C), neuroblasts (A), and endothelial cells (bv). EGFR is expressed by the subpopulation of VEGFR-3 NSCs. VEGF-C produced by SVZ astrocytes and also other cell types promotes activation of VEGFR-3-expressing cells (i.e., niche astrocytes and NSCs), which increase in number following overexpression of VEGF-C. This enhances the number of TAPs and neuroblasts.
Figure Legend Snippet: Proposed model for VEGF-C and VEGFR-3 function in the adult SVZ. VEGFR-3 is expressed by a subpopulation of astrocytes (B) and almost all NSCs (B1), but not by the majority of TAPs (C), neuroblasts (A), and endothelial cells (bv). EGFR is expressed by the subpopulation of VEGFR-3 NSCs. VEGF-C produced by SVZ astrocytes and also other cell types promotes activation of VEGFR-3-expressing cells (i.e., niche astrocytes and NSCs), which increase in number following overexpression of VEGF-C. This enhances the number of TAPs and neuroblasts.

Techniques Used: Produced, Activation Assay, Expressing, Over Expression

5) Product Images from "MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis"

Article Title: MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis

Journal: Cancer Cell International

doi: 10.1186/1475-2867-13-98

The correlation between MT1-MMP and VEGF-C expression in human breast cancer cells. (a) MT1-MMP and VEGF-C mRNA expression. (b) MT1-MMP and VEGF-C protein expression.
Figure Legend Snippet: The correlation between MT1-MMP and VEGF-C expression in human breast cancer cells. (a) MT1-MMP and VEGF-C mRNA expression. (b) MT1-MMP and VEGF-C protein expression.

Techniques Used: Expressing

6) Product Images from "Implication of vascular endothelial growth factor A and C in revealing diagnostic lymphangiogenic markers in node-positive bladder cancer"

Article Title: Implication of vascular endothelial growth factor A and C in revealing diagnostic lymphangiogenic markers in node-positive bladder cancer

Journal: Oncotarget

doi: 10.18632/oncotarget.15669

SLP-76 expression and production in MEC A . The SLP-76 gene expression upon VEGF stimulation (at either concentration) and under bladder cell line trans-well assay was compared with SLP-76 gene expression elicited in anti-CD3 treated CD8 T cells (positive control). SLP-76-fold increase after direct MEC stimulation or priming with cell lines was calculated as gene expression 2-fold over its constitutive expression (arbitrary cut-off). SLP-76-fold increase in anti CD3 treated CD8 T cells was calculated as gene expression 2-fold over SLP-76 gene expression in non-stimulated CD8. Error bars represent the mean ± SD of three replicates. B . Representative fluorescent-activated cell sorting (FACS) dot plot analysis of in vitro SLP-76 intracellular staining in anti-CD3 treated CD8 T cells (upper right panel) and VEGF-C (2 ng/ml medium right panel) or VEGF-A (17 ng/ml lower right panel) stimulated CD31+ MEC, as compared with isotype staining (all left panels). Overlaid histograms refer to mean fluorescent intensity (MFI) of in vitro SLP-76 intracellular staining of CD8 T cells upon anti-CD3 induction (above) or MEC after VEGF-C (middle) or VEGF–A (below) stimulation compared with isotype staining. C . Western blot showing SLP-76 expression in MEC stimulated with either VEGF-A or VEGF-C at 50 ng/ml, over 3 and 6 h, as compared with SLP-76 expression in human CD8 T cells treated with anti-CD3 mAb (CD8 vs αCD3). D . A representative image by fluorescence microscopy (scale bar 400 μm) of MEC after 3 and 6 h of stimulation with either VEGF-A or VEGF-C (50 ng/ml). The SLP-76 basal staining (no stimulation) for both cell lines (MEC right and LEC left) is also reported. MEC were immunostained for SLP-76 (Cy-3; red), cytoskeleton (phalloidin; green), and nucleus (DAPI; blue). E . MEC migration was assessed by a scratch wound healing assay, as shown in one representative picture (scale bar 50 μm) by confocal microscopy after 24 h of VEGF-C stimulation at the standard concentration. Cells were cultured in 6-well plates and allowed to reach 80% confluence before scratching vertically and horizontally (see symbol bottom right in the upper panel). Histograms represent migration potential of cells based on the quantification of empty area (% closure rate) as compared with time 0 (100% empty area) for VEGF-A (gray bar) or VEGF-C (black bar) (p
Figure Legend Snippet: SLP-76 expression and production in MEC A . The SLP-76 gene expression upon VEGF stimulation (at either concentration) and under bladder cell line trans-well assay was compared with SLP-76 gene expression elicited in anti-CD3 treated CD8 T cells (positive control). SLP-76-fold increase after direct MEC stimulation or priming with cell lines was calculated as gene expression 2-fold over its constitutive expression (arbitrary cut-off). SLP-76-fold increase in anti CD3 treated CD8 T cells was calculated as gene expression 2-fold over SLP-76 gene expression in non-stimulated CD8. Error bars represent the mean ± SD of three replicates. B . Representative fluorescent-activated cell sorting (FACS) dot plot analysis of in vitro SLP-76 intracellular staining in anti-CD3 treated CD8 T cells (upper right panel) and VEGF-C (2 ng/ml medium right panel) or VEGF-A (17 ng/ml lower right panel) stimulated CD31+ MEC, as compared with isotype staining (all left panels). Overlaid histograms refer to mean fluorescent intensity (MFI) of in vitro SLP-76 intracellular staining of CD8 T cells upon anti-CD3 induction (above) or MEC after VEGF-C (middle) or VEGF–A (below) stimulation compared with isotype staining. C . Western blot showing SLP-76 expression in MEC stimulated with either VEGF-A or VEGF-C at 50 ng/ml, over 3 and 6 h, as compared with SLP-76 expression in human CD8 T cells treated with anti-CD3 mAb (CD8 vs αCD3). D . A representative image by fluorescence microscopy (scale bar 400 μm) of MEC after 3 and 6 h of stimulation with either VEGF-A or VEGF-C (50 ng/ml). The SLP-76 basal staining (no stimulation) for both cell lines (MEC right and LEC left) is also reported. MEC were immunostained for SLP-76 (Cy-3; red), cytoskeleton (phalloidin; green), and nucleus (DAPI; blue). E . MEC migration was assessed by a scratch wound healing assay, as shown in one representative picture (scale bar 50 μm) by confocal microscopy after 24 h of VEGF-C stimulation at the standard concentration. Cells were cultured in 6-well plates and allowed to reach 80% confluence before scratching vertically and horizontally (see symbol bottom right in the upper panel). Histograms represent migration potential of cells based on the quantification of empty area (% closure rate) as compared with time 0 (100% empty area) for VEGF-A (gray bar) or VEGF-C (black bar) (p

Techniques Used: Expressing, Concentration Assay, Positive Control, FACS, In Vitro, Staining, Western Blot, Fluorescence, Microscopy, Migration, Wound Healing Assay, Confocal Microscopy, Cell Culture

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Angiogenesis and lymphangiogenesis as prognostic factors after therapy in patients with cervical cancer
Article Snippet: .. VEGF-C was determined by means of a quantitative sandwich enzyme immunoassay ELISA using a human antibody VEGF-C ELISA produced by Bender MedSystem, enzyme-linked immunosorbent detecting the activity of human VEGF-C in body fluids. .. The measure for the intensity of angiogenesis and lymphangiogenesis in immunohistochemical reactions is the number of visible blood vessels within the tumour.

Article Title: Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation
Article Snippet: .. VEGF-C and VEGF-D in the lungs of nude mice treated intranasally with adenoviruses encoding LacZ, VEGF165, VEGF-C, or VEGF-D ΔNΔC 10 days previously were measured by ELISA (human VEGF-C ELISA [no. BMS297; Bender MedSystems], human VEGF-D ELISA [no. DVED00; R & D Systems]). .. Full-length recombinant human VEGF-C and supernatant from HeLa cells transfected with adenoviral VEGF-D ΔNΔC were used as positive controls.

Article Title: MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis
Article Snippet: .. ELISA for VEGF-C Concentrations of VEGF-C in the medium of the cultured tumour cells were determined using a VEGF-C ELISA kit, as described in the protocol provided by the manufacturer (Bender MedSystems, San Diego, CA, USA). .. Briefly, cell culture medium was diluted 50-fold with sample diluent and added into wells of a microwell plate coated with anti-VEGF-C polyclonal antibody and incubated for 2 h at room temperature.

Article Title: Angiogenesis and lymphangiogenesis as prognostic factors after therapy in patients with cervical cancer
Article Snippet: .. VEGF-C was determined by means of a quantitative sandwich enzyme immunoassay using a human antibody VEGF-C ELISA produced by Bender MedSystem, enzyme-linked immunosorbent detecting the activity of human VEGF-C in body fluids. .. The measure for the intensity of angiogenesis and lymphangiogenesis in immunohistochemical reactions is the number of blood vessels within the tumour.

Article Title: Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis
Article Snippet: .. These lysates were used to detect VEGF-C with an ELISA kit (Bender MedSystems, BMS626) according to manufacturer's protocol. .. Measurement of the color readout was performed at 450 nm with a multiscan microplate reader (Thermo Electron Corporation).

Article Title: Implication of vascular endothelial growth factor A and C in revealing diagnostic lymphangiogenic markers in node-positive bladder cancer
Article Snippet: .. Protein analysis and expression The production of VEGF-A, VEGF-C and VEGF-D proteins in supernatants of bladder cell lines was analyzed by using either the human VEGF-A enzyme-linked immunosorbent assay (ELISA) Kit (BMS277) or the human VEGF-C ELISA Kit (BMS297; Bender MedSystems, Wien, Austria) according to the manufacturer's instructions. .. The detection of VEGF-D was analyzed by human VEGF-D ELISA Kit (LS-F27835; LSBio.

Cell Culture:

Article Title: MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis
Article Snippet: .. ELISA for VEGF-C Concentrations of VEGF-C in the medium of the cultured tumour cells were determined using a VEGF-C ELISA kit, as described in the protocol provided by the manufacturer (Bender MedSystems, San Diego, CA, USA). .. Briefly, cell culture medium was diluted 50-fold with sample diluent and added into wells of a microwell plate coated with anti-VEGF-C polyclonal antibody and incubated for 2 h at room temperature.

Mouse Assay:

Article Title: Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation
Article Snippet: .. VEGF-C and VEGF-D in the lungs of nude mice treated intranasally with adenoviruses encoding LacZ, VEGF165, VEGF-C, or VEGF-D ΔNΔC 10 days previously were measured by ELISA (human VEGF-C ELISA [no. BMS297; Bender MedSystems], human VEGF-D ELISA [no. DVED00; R & D Systems]). .. Full-length recombinant human VEGF-C and supernatant from HeLa cells transfected with adenoviral VEGF-D ΔNΔC were used as positive controls.

Produced:

Article Title: Angiogenesis and lymphangiogenesis as prognostic factors after therapy in patients with cervical cancer
Article Snippet: .. VEGF-C was determined by means of a quantitative sandwich enzyme immunoassay ELISA using a human antibody VEGF-C ELISA produced by Bender MedSystem, enzyme-linked immunosorbent detecting the activity of human VEGF-C in body fluids. .. The measure for the intensity of angiogenesis and lymphangiogenesis in immunohistochemical reactions is the number of visible blood vessels within the tumour.

Article Title: Angiogenesis and lymphangiogenesis as prognostic factors after therapy in patients with cervical cancer
Article Snippet: .. VEGF-C was determined by means of a quantitative sandwich enzyme immunoassay using a human antibody VEGF-C ELISA produced by Bender MedSystem, enzyme-linked immunosorbent detecting the activity of human VEGF-C in body fluids. .. The measure for the intensity of angiogenesis and lymphangiogenesis in immunohistochemical reactions is the number of blood vessels within the tumour.

Activity Assay:

Article Title: Angiogenesis and lymphangiogenesis as prognostic factors after therapy in patients with cervical cancer
Article Snippet: .. VEGF-C was determined by means of a quantitative sandwich enzyme immunoassay ELISA using a human antibody VEGF-C ELISA produced by Bender MedSystem, enzyme-linked immunosorbent detecting the activity of human VEGF-C in body fluids. .. The measure for the intensity of angiogenesis and lymphangiogenesis in immunohistochemical reactions is the number of visible blood vessels within the tumour.

Article Title: Angiogenesis and lymphangiogenesis as prognostic factors after therapy in patients with cervical cancer
Article Snippet: .. VEGF-C was determined by means of a quantitative sandwich enzyme immunoassay using a human antibody VEGF-C ELISA produced by Bender MedSystem, enzyme-linked immunosorbent detecting the activity of human VEGF-C in body fluids. .. The measure for the intensity of angiogenesis and lymphangiogenesis in immunohistochemical reactions is the number of blood vessels within the tumour.

Expressing:

Article Title: Implication of vascular endothelial growth factor A and C in revealing diagnostic lymphangiogenic markers in node-positive bladder cancer
Article Snippet: .. Protein analysis and expression The production of VEGF-A, VEGF-C and VEGF-D proteins in supernatants of bladder cell lines was analyzed by using either the human VEGF-A enzyme-linked immunosorbent assay (ELISA) Kit (BMS277) or the human VEGF-C ELISA Kit (BMS297; Bender MedSystems, Wien, Austria) according to the manufacturer's instructions. .. The detection of VEGF-D was analyzed by human VEGF-D ELISA Kit (LS-F27835; LSBio.

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    Bender MedSystems vegf c
    <t>VEGF-C</t> and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,
    Vegf C, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf c/product/Bender MedSystems
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    vegf c - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    88
    Bender MedSystems vegf c protein
    Effect of <t>VEGF-C</t> overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p
    Vegf C Protein, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf c protein/product/Bender MedSystems
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf c protein - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    89
    Bender MedSystems human vegf c
    Dynamic NIRF imaging of ICG liposome injected B16-luc2 tumor bearing mice Representative figures of lymphatic drainage patterns of LP-ICG after intradermal injection (A) from pcDNA, <t>VEGF-C</t> low metastatic, and VEGF-C high metastatic mice. Black circles: popliteal lymph node; red squares: liver; grey triangles: medial iliac lymph node. Correlation plot (B) showing K LN rates through popliteal lymph node versus popliteal lymph node luciferase signals. Dashed line represents threshold between low metastatic and high metastatic VEGF-C tumor bearing mice. K LN rates (C) and half life measurements (D) of normal, pcDNA tumor, VEGF-C low metastatic and VEGF-C high metastatic groups. * p
    Human Vegf C, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human vegf c/product/Bender MedSystems
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human vegf c - by Bioz Stars, 2020-09
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    Image Search Results


    VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,

    Journal: Journal of Clinical Investigation

    Article Title: Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation

    doi: 10.1172/JCI200522037

    Figure Lengend Snippet: VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,

    Article Snippet: VEGF-C and VEGF-D in the lungs of nude mice treated intranasally with adenoviruses encoding LacZ, VEGF165, VEGF-C, or VEGF-D ΔNΔC 10 days previously were measured by ELISA (human VEGF-C ELISA [no. BMS297; Bender MedSystems], human VEGF-D ELISA [no. DVED00; R & D Systems]).

    Techniques: Infection, Immunohistochemistry, Staining

    The correlation between MT1-MMP and VEGF-C expression in human breast cancer cells. (a) MT1-MMP and VEGF-C mRNA expression. (b) MT1-MMP and VEGF-C protein expression.

    Journal: Cancer Cell International

    Article Title: MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis

    doi: 10.1186/1475-2867-13-98

    Figure Lengend Snippet: The correlation between MT1-MMP and VEGF-C expression in human breast cancer cells. (a) MT1-MMP and VEGF-C mRNA expression. (b) MT1-MMP and VEGF-C protein expression.

    Article Snippet: ELISA for VEGF-C Concentrations of VEGF-C in the medium of the cultured tumour cells were determined using a VEGF-C ELISA kit, as described in the protocol provided by the manufacturer (Bender MedSystems, San Diego, CA, USA).

    Techniques: Expressing

    Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Over Expression, Staining

    Effect of VEGFR3-Ig treatment on orthotopic PC-3/VEGF-C tumors . VEGFR3-Ig fusion protein-producing construct or lacZ-Ig controls were injected intravenously into mice five minutes before orthotopic inoculation of PC-3/VEGF-C cells. The density of m-LYVE-1 positive lymphatic capillaries was decreased in VEGFR3-Ig-treated tumors (23 ± 4 μm/mm 2 , n = 8 vs . 123 ± 7 μm/mm 2 , n = 6, p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGFR3-Ig treatment on orthotopic PC-3/VEGF-C tumors . VEGFR3-Ig fusion protein-producing construct or lacZ-Ig controls were injected intravenously into mice five minutes before orthotopic inoculation of PC-3/VEGF-C cells. The density of m-LYVE-1 positive lymphatic capillaries was decreased in VEGFR3-Ig-treated tumors (23 ± 4 μm/mm 2 , n = 8 vs . 123 ± 7 μm/mm 2 , n = 6, p

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Construct, Injection, Mouse Assay

    Ectopic expression of VEGF-C in PC-3 cells and orthotopic tumors . PC-3 cells were stably transfected with a vector containing human VEGF-C (PC-3/VEGF-C) or empty vector (mock). One cell clone expressing VEGF-C at a high level, and one control clone (PC-3/mock) were selected for further studies. ELISA analysis of VEGF-C protein concentration in cell lysates (A) and in conditioned media (B) revealed increased expression in PC-3/VEGF-C cells compared with PC-3/mock cells. Data are expressed as means ± SD ng/mL, corresponding 10 6 cells. (C) Western blot analysis revealed that PC-3 cell clones expressed mainly 29/31 kD form of VEGF-C. Detectable levels of 21 kD form were not seen. β-actin control was from cell lysates corresponding to the collected conditioned medium.

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Ectopic expression of VEGF-C in PC-3 cells and orthotopic tumors . PC-3 cells were stably transfected with a vector containing human VEGF-C (PC-3/VEGF-C) or empty vector (mock). One cell clone expressing VEGF-C at a high level, and one control clone (PC-3/mock) were selected for further studies. ELISA analysis of VEGF-C protein concentration in cell lysates (A) and in conditioned media (B) revealed increased expression in PC-3/VEGF-C cells compared with PC-3/mock cells. Data are expressed as means ± SD ng/mL, corresponding 10 6 cells. (C) Western blot analysis revealed that PC-3 cell clones expressed mainly 29/31 kD form of VEGF-C. Detectable levels of 21 kD form were not seen. β-actin control was from cell lysates corresponding to the collected conditioned medium.

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Protein Concentration, Western Blot, Clone Assay

    Effect of VEGF-C overexpression on PC-3 prostate tumor growth . The occurrence of subcutaneous tumors of both PC-3/VEGF-C ( n = 8) and PC-3/mock ( n = 12) was 100%. (A) Subcutaneous PC-3/VEGF-C tumors grew larger (1132 ± 34 mm 3 , gray bars) than PC-3/mock tumors (47 ± 7 mm 3 , black bars) at 4 weeks and the differences in tumor volumes between the groups were significant at all time points between 2 and 4 weeks ( p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGF-C overexpression on PC-3 prostate tumor growth . The occurrence of subcutaneous tumors of both PC-3/VEGF-C ( n = 8) and PC-3/mock ( n = 12) was 100%. (A) Subcutaneous PC-3/VEGF-C tumors grew larger (1132 ± 34 mm 3 , gray bars) than PC-3/mock tumors (47 ± 7 mm 3 , black bars) at 4 weeks and the differences in tumor volumes between the groups were significant at all time points between 2 and 4 weeks ( p

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Over Expression

    Effect of ectopic VEGF-C on lymphatic vessel density of orthotopic PC-3 tumors . M-LYVE-1-positive staining was observed primarily in the tumor periphery and in the peritumoral area (A-B). No differences were detected in the density of lymphatic vessels Bar 500 μm.

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of ectopic VEGF-C on lymphatic vessel density of orthotopic PC-3 tumors . M-LYVE-1-positive staining was observed primarily in the tumor periphery and in the peritumoral area (A-B). No differences were detected in the density of lymphatic vessels Bar 500 μm.

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Staining

    Metastasis of PC-3/VEGF-C tumors to prostate-draining lymph nodes and lungs . The prostate draining lymph nodes and the lungs were cut through to detect metastasis. Altogether 8-10 lymph nodes and six lung sections/mouse were studied and number of metastases was counted. The relative metastatic area of lymph nodes was determined by histomorphometry but there were no statistically significant differences (data not shown). The occurrence of metastasis in lymph nodes was 21% ( n = 6 mice out of 29 tumor-bearing mice) in the PC-3/VEGF-C group and 58% ( n = 14 mice out of 24 tumor-bearing mice) in the PC-3/mock group and in lungs was 48% ( n = 14 out of 29 tumor bearing mice) in the PC-3/VEGF-C group and 8% ( n = 2 out of 24 tumor-bearing mice) in the PC-3/mock group.

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Metastasis of PC-3/VEGF-C tumors to prostate-draining lymph nodes and lungs . The prostate draining lymph nodes and the lungs were cut through to detect metastasis. Altogether 8-10 lymph nodes and six lung sections/mouse were studied and number of metastases was counted. The relative metastatic area of lymph nodes was determined by histomorphometry but there were no statistically significant differences (data not shown). The occurrence of metastasis in lymph nodes was 21% ( n = 6 mice out of 29 tumor-bearing mice) in the PC-3/VEGF-C group and 58% ( n = 14 mice out of 24 tumor-bearing mice) in the PC-3/mock group and in lungs was 48% ( n = 14 out of 29 tumor bearing mice) in the PC-3/VEGF-C group and 8% ( n = 2 out of 24 tumor-bearing mice) in the PC-3/mock group.

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Mouse Assay

    Dynamic NIRF imaging of ICG liposome injected B16-luc2 tumor bearing mice Representative figures of lymphatic drainage patterns of LP-ICG after intradermal injection (A) from pcDNA, VEGF-C low metastatic, and VEGF-C high metastatic mice. Black circles: popliteal lymph node; red squares: liver; grey triangles: medial iliac lymph node. Correlation plot (B) showing K LN rates through popliteal lymph node versus popliteal lymph node luciferase signals. Dashed line represents threshold between low metastatic and high metastatic VEGF-C tumor bearing mice. K LN rates (C) and half life measurements (D) of normal, pcDNA tumor, VEGF-C low metastatic and VEGF-C high metastatic groups. * p

    Journal: Cancer Research

    Article Title: Quantitative Imaging of Lymphatic Function with Liposomal Indocyanine Green

    doi: 10.1158/0008-5472.CAN-10-0271

    Figure Lengend Snippet: Dynamic NIRF imaging of ICG liposome injected B16-luc2 tumor bearing mice Representative figures of lymphatic drainage patterns of LP-ICG after intradermal injection (A) from pcDNA, VEGF-C low metastatic, and VEGF-C high metastatic mice. Black circles: popliteal lymph node; red squares: liver; grey triangles: medial iliac lymph node. Correlation plot (B) showing K LN rates through popliteal lymph node versus popliteal lymph node luciferase signals. Dashed line represents threshold between low metastatic and high metastatic VEGF-C tumor bearing mice. K LN rates (C) and half life measurements (D) of normal, pcDNA tumor, VEGF-C low metastatic and VEGF-C high metastatic groups. * p

    Article Snippet: We detected 0.8 ng/ml of human VEGF-C in the cell culture supernatant of the stably VEGF-C transfected clone that was used for further studies, as assessed by quantitative ELISA (Bender MedSystems, Vienna, Austria) at 24 hours after seeding 1×105 cells.

    Techniques: Imaging, Injection, Mouse Assay, Luciferase

    Bioluminescent imaging of popliteal lymph node metastases in human VEGF-C overexpressing B16-luc2 melanoma tumor bearing mice (A) Tumor volumes of VEGF-C expressing and control cells at 21 days. Quantification of in vivo bioluminescent signals from 9 VEGF-C and 8 pcDNA tumor bearing mice (B). Bioluminescent images of popliteal lymph node region revealing lymph node metastasis in representative VEGF-C tumor bearing mouse (C) and lack of signal in pcDNA tumor bearing mouse (D). Insets: Ex vivo signal of dissected popliteal lymph nodes from VEGF-C (C) or pcDNA (D) tumor bearing mice. * p

    Journal: Cancer Research

    Article Title: Quantitative Imaging of Lymphatic Function with Liposomal Indocyanine Green

    doi: 10.1158/0008-5472.CAN-10-0271

    Figure Lengend Snippet: Bioluminescent imaging of popliteal lymph node metastases in human VEGF-C overexpressing B16-luc2 melanoma tumor bearing mice (A) Tumor volumes of VEGF-C expressing and control cells at 21 days. Quantification of in vivo bioluminescent signals from 9 VEGF-C and 8 pcDNA tumor bearing mice (B). Bioluminescent images of popliteal lymph node region revealing lymph node metastasis in representative VEGF-C tumor bearing mouse (C) and lack of signal in pcDNA tumor bearing mouse (D). Insets: Ex vivo signal of dissected popliteal lymph nodes from VEGF-C (C) or pcDNA (D) tumor bearing mice. * p

    Article Snippet: We detected 0.8 ng/ml of human VEGF-C in the cell culture supernatant of the stably VEGF-C transfected clone that was used for further studies, as assessed by quantitative ELISA (Bender MedSystems, Vienna, Austria) at 24 hours after seeding 1×105 cells.

    Techniques: Imaging, Mouse Assay, Expressing, In Vivo, Ex Vivo