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R&D Systems vegf a165 b elisa
Pan–vascular endothelial growth factor <t>(VEGF)-A</t> and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b <t>ELISA</t> data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P
Vegf A165 B Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Differential Expression of VEGF-Axxx Isoforms Is Critical for Development of Pulmonary Fibrosis

Journal: American Journal of Respiratory and Critical Care Medicine

doi: 10.1164/rccm.201603-0568OC

Pan–vascular endothelial growth factor (VEGF)-A and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b ELISA data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P
Figure Legend Snippet: Pan–vascular endothelial growth factor (VEGF)-A and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b ELISA data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Marker, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

The effect of vascular endothelial growth factor (VEGF)-A 165 b on the development of pulmonary fibrosis. ( A ) Lung phenotype of the MMTV-VEGF-A 165 b transgenic (TG) mouse. Immunohistochemical staining for VEGF-A 165 b in the lung of TG mice with quantification of VEGF-A 165 b expression by ELISA, in whole-tissue lysates and bronchoalveolar lavage fluid (BALF). VEGF-A 165 b expression was increased in the alveolar type II (ATII) cells of the TG mouse lung ( d and f , indicated by arrows ) compared with the wild-type (WT) lung ( c and e ). Isotype IgG staining was used as a negative control ( a and b ). Images were taken at ×100 magnification; scale bars = 10 μm, (n = 3, n = 1 shown). VEGF-A 165 b expression was significantly up-regulated in whole-tissue lysates of TG mice compared with WT mice (* P
Figure Legend Snippet: The effect of vascular endothelial growth factor (VEGF)-A 165 b on the development of pulmonary fibrosis. ( A ) Lung phenotype of the MMTV-VEGF-A 165 b transgenic (TG) mouse. Immunohistochemical staining for VEGF-A 165 b in the lung of TG mice with quantification of VEGF-A 165 b expression by ELISA, in whole-tissue lysates and bronchoalveolar lavage fluid (BALF). VEGF-A 165 b expression was increased in the alveolar type II (ATII) cells of the TG mouse lung ( d and f , indicated by arrows ) compared with the wild-type (WT) lung ( c and e ). Isotype IgG staining was used as a negative control ( a and b ). Images were taken at ×100 magnification; scale bars = 10 μm, (n = 3, n = 1 shown). VEGF-A 165 b expression was significantly up-regulated in whole-tissue lysates of TG mice compared with WT mice (* P

Techniques Used: Transgenic Assay, Immunohistochemistry, Staining, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control

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    R&D Systems vegf a165 b elisa
    Pan–vascular endothelial growth factor <t>(VEGF)-A</t> and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b <t>ELISA</t> data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P
    Vegf A165 B Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf a165 b elisa/product/R&D Systems
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf a165 b elisa - by Bioz Stars, 2021-09
    97/100 stars
      Buy from Supplier

    93
    R&D Systems vegf a165 b
    Pan–vascular endothelial growth factor <t>(VEGF)-A</t> and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b <t>ELISA</t> data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P
    Vegf A165 B, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf a165 b/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf a165 b - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    92
    R&D Systems mouse anti human antibody against vegf a165 b
    Pan–vascular endothelial growth factor <t>(VEGF)-A</t> and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b <t>ELISA</t> data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P
    Mouse Anti Human Antibody Against Vegf A165 B, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human antibody against vegf a165 b/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human antibody against vegf a165 b - by Bioz Stars, 2021-09
    92/100 stars
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    Pan–vascular endothelial growth factor (VEGF)-A and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b ELISA data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Differential Expression of VEGF-Axxx Isoforms Is Critical for Development of Pulmonary Fibrosis

    doi: 10.1164/rccm.201603-0568OC

    Figure Lengend Snippet: Pan–vascular endothelial growth factor (VEGF)-A and VEGF-A xxx b expression in the lungs of patients with idiopathic pulmonary fibrosis (IPF). ( A ) Pan-VEGF-A reverse transcription–polymerase chain reaction (RT-PCR) of whole-lung RNA extract using VEGF-A Exon2/3 For and 8b Rev primers ( top ) and Exon 7a For and 8b Rev primers ( bottom ) (n = 5 normal lung; n = 5 IPF lung). VEGF-A 121 a, VEGF-A 165 a, VEGF-A 165 b, and VEGF-A 189 a isoforms were identified by RT-PCR and verified by direct sequencing ( see Figure E1). L = 50-bp marker. ( B ) Quantitative RT-PCR of pan-VEGF-A and VEGF-A xxx b mRNA expression in whole-lung tissue homogenates of normal (n = 5) and IPF lung (n = 5). No significant difference was detected in the expression of pan-VEGF-A or VEGF-A 165 b (unpaired Student’s t test) isoforms between normal and IPF lung samples. ( C ) Pan-VEGF-A and VEGF-A 165 b ELISA data from lung whole-tissue lysates in normal (n = 5) and IPF (n = 5) subjects. There was no significant difference in pan-VEGF-A expression (unpaired Student’s t test with Welch correction), but a significant increase in VEGF-A 165 b expression in the IPF lung was observed (**** P

    Article Snippet: Pan-VEGF-A and VEGF-A165 b levels were quantified using Pan-VEGF-A and VEGF-A165 b ELISA sets (R & D Systems, Oxford, UK).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Marker, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    The effect of vascular endothelial growth factor (VEGF)-A 165 b on the development of pulmonary fibrosis. ( A ) Lung phenotype of the MMTV-VEGF-A 165 b transgenic (TG) mouse. Immunohistochemical staining for VEGF-A 165 b in the lung of TG mice with quantification of VEGF-A 165 b expression by ELISA, in whole-tissue lysates and bronchoalveolar lavage fluid (BALF). VEGF-A 165 b expression was increased in the alveolar type II (ATII) cells of the TG mouse lung ( d and f , indicated by arrows ) compared with the wild-type (WT) lung ( c and e ). Isotype IgG staining was used as a negative control ( a and b ). Images were taken at ×100 magnification; scale bars = 10 μm, (n = 3, n = 1 shown). VEGF-A 165 b expression was significantly up-regulated in whole-tissue lysates of TG mice compared with WT mice (* P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Differential Expression of VEGF-Axxx Isoforms Is Critical for Development of Pulmonary Fibrosis

    doi: 10.1164/rccm.201603-0568OC

    Figure Lengend Snippet: The effect of vascular endothelial growth factor (VEGF)-A 165 b on the development of pulmonary fibrosis. ( A ) Lung phenotype of the MMTV-VEGF-A 165 b transgenic (TG) mouse. Immunohistochemical staining for VEGF-A 165 b in the lung of TG mice with quantification of VEGF-A 165 b expression by ELISA, in whole-tissue lysates and bronchoalveolar lavage fluid (BALF). VEGF-A 165 b expression was increased in the alveolar type II (ATII) cells of the TG mouse lung ( d and f , indicated by arrows ) compared with the wild-type (WT) lung ( c and e ). Isotype IgG staining was used as a negative control ( a and b ). Images were taken at ×100 magnification; scale bars = 10 μm, (n = 3, n = 1 shown). VEGF-A 165 b expression was significantly up-regulated in whole-tissue lysates of TG mice compared with WT mice (* P

    Article Snippet: Pan-VEGF-A and VEGF-A165 b levels were quantified using Pan-VEGF-A and VEGF-A165 b ELISA sets (R & D Systems, Oxford, UK).

    Techniques: Transgenic Assay, Immunohistochemistry, Staining, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control