Vegf A 165, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Identification of a metalloprotease-chemokine signaling system in the ovarian cancer microenvironment: implications for anti-angiogenic therapy"
Article Title: Identification of a metalloprotease-chemokine signaling system in the ovarian cancer microenvironment: implications for anti-angiogenic therapy
Journal: Cancer research
Figure Legend Snippet: MMP-1-PAR1 stimulates secretion of CXCR1/2 chemokines from ovarian carcinoma cells. A , OVCAR-4 cells were stimulated with either 1 nM MMP-1 or PBS buffer in RPMI with 0.1% BSA and conditioned media (CM) was collected after 18 h. The angiogenesis array membranes were incubated with CM from the OVCAR-4 cells and fold change of the spot intensities was quantified using NIH ImageJ software. B ) in OVCAR-4, IGROV-1, OVCAR-3 and OVCAR-4/PAR1 shRNAi ovarian carcinoma cell lines. C–E, ELISA analysis was used to measure IL-8 (C), GRO-α (D), and VEGF-A-165 (E) levels in CM from various ovarian carcinoma cell lines that were stimulated with 1 nM MMP-1 in the presence or absence of the PAR1 antagonist pepducin P1pal-7 (3 μM), the small molecule PAR1 antagonist RWJ-56110 (5 μM), or buffer control as indicated. Data (mean ± SE) are from 2–4 experiments performed in duplicate.
Techniques Used: Incubation, Software, Enzyme-linked Immunosorbent Assay
Article Title: Growth Factor–like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells
Article Snippet: For cytokine combination experiments and TGF-β3 dose response, cells were seeded at 5 × 105 per ml in media supplemented with IL-6 (10 ng/ml), IL-21 (50 ng/ml), IFN-α (100 U/ml), and combinations of Jagged 1 (2 μg/ml); Osteopontin (1 μg/ml); Osteoprotegerin, SCF, and IGF-BP2 (100 ng/ml); Activin A, and M-CSF (50 ng/ml); IGF-II (30 ng/ml): ANGPT1, VEGF-165, VEGF-121, MIF, and TGF-β3 (10 ng/ml or 0.1–1000 ng/ml for dose response).
Article Title: Heparan sulfate functions are altered in the osteoarthritic cartilage
Article Snippet: Then, HBP were added to each well (FGF2 at 2.5 ng/well; VEGF-165 at 5 ng/well) together with increasing concentrations of GAG extracted from the samples (serial dilutions from 0.01 to 10 μg/mL in PBS) in duplicate.
Article Title: Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells
Article Snippet: VEGF-A 165 (VEGF) and
Article Title: Dual role of fatty acid-binding protein 5 on endothelial cell fate: a potential link between lipid metabolism and angiogenic responses
Article Snippet: In some experiments, cells were treated with
Article Title: Endothelial cell-fatty acid binding protein 4 promotes angiogenesis: role of stem cell factor/c-kit pathway
Article Snippet: HEK-293T cells (ATCC, Rockville, MD, USA) were cultured in DMEM (Invitrogen) supplemented with 10 % FBS and 1× non-essential amino acids (Sigma). .. VEGF-A 165 (VEGF), mouse basic FGF (bFGF), and
Article Title: Dll4-containing exosomes induce capillary sprout retraction in a 3D microenvironment
Article Snippet: We transduced U-87 tumor cell line with human Dll4 in pLVX-IRES-ZsGreen lentiviral vector to overexpress Dll4. .. Recombinant human Dll4 and
Article Title: Granzyme B Releases Vascular Endothelial Growth Factor from Extracellular Matrix and Induces Vascular Permeability
Article Snippet: ..