Structured Review

TaKaRa vector pmd19t simple
Vector Pmd19t Simple, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector pmd19t simple/product/TaKaRa
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
vector pmd19t simple - by Bioz Stars, 2020-08
85/100 stars

Related Products / Commonly Used Together

double-jointed pcr
735-bp pcr
primers tub2gf/tub2gr

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Related Articles

Polymerase Chain Reaction:

Article Title: A single-nucleotide-polymorphism-based genotyping assay for simultaneous detection of different carbendazim-resistant genotypes in the Fusarium graminearum species complex
Article Snippet: .. The two fragments were joined by double-jointed PCR using primers Tub2GF/Tub2GR and cloned into cloning vector pMD19T-Simple (TaKaRa) to generate pMD-198L. .. Using primers 198KF/198KR instead of 198LF/198LR, the same procedure was used to construct the vector pMD-198K containing the E198K mutation.

Article Title: Characterization of a cryptic plasmid pSM429 and its application for heterologous expression in psychrophilic Pseudoalteromonas
Article Snippet: .. The 735-bp PCR product was cloned into vector pMD19T simple (Takara). .. The fragment of the promoter-less ermC gene in the pMD19T was separated with Pst I and Sal I and was used in the construction of the ermC -expression vector with the smallest stable plasmid pWD2.

Clone Assay:

Article Title: A single-nucleotide-polymorphism-based genotyping assay for simultaneous detection of different carbendazim-resistant genotypes in the Fusarium graminearum species complex
Article Snippet: .. The two fragments were joined by double-jointed PCR using primers Tub2GF/Tub2GR and cloned into cloning vector pMD19T-Simple (TaKaRa) to generate pMD-198L. .. Using primers 198KF/198KR instead of 198LF/198LR, the same procedure was used to construct the vector pMD-198K containing the E198K mutation.

Article Title: Characterization of a cryptic plasmid pSM429 and its application for heterologous expression in psychrophilic Pseudoalteromonas
Article Snippet: .. The 735-bp PCR product was cloned into vector pMD19T simple (Takara). .. The fragment of the promoter-less ermC gene in the pMD19T was separated with Pst I and Sal I and was used in the construction of the ermC -expression vector with the smallest stable plasmid pWD2.

Plasmid Preparation:

Article Title: A single-nucleotide-polymorphism-based genotyping assay for simultaneous detection of different carbendazim-resistant genotypes in the Fusarium graminearum species complex
Article Snippet: .. The two fragments were joined by double-jointed PCR using primers Tub2GF/Tub2GR and cloned into cloning vector pMD19T-Simple (TaKaRa) to generate pMD-198L. .. Using primers 198KF/198KR instead of 198LF/198LR, the same procedure was used to construct the vector pMD-198K containing the E198K mutation.

Article Title: Rational Design of Disulfide Bonds Increases Thermostability of a Mesophilic 1,3-1,4-β-Glucanase from Bacillus terquilensis
Article Snippet: .. The restriction enzymes (Bam HI and Xho I) and the vector pMD19T-simple were purchased from Takara (Dalian, China). .. The vector pET28a(+) (Invitrogen, Shanghai, China) and E . coli BL21(DE3) cells were used for recombinant plasmid construction and expression.

Article Title: Characterization of a cryptic plasmid pSM429 and its application for heterologous expression in psychrophilic Pseudoalteromonas
Article Snippet: .. The 735-bp PCR product was cloned into vector pMD19T simple (Takara). .. The fragment of the promoter-less ermC gene in the pMD19T was separated with Pst I and Sal I and was used in the construction of the ermC -expression vector with the smallest stable plasmid pWD2.

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    TaKaRa pmd19t simple vector
    Pmd19t Simple Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19t simple vector/product/TaKaRa
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pmd19t simple vector - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

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