vector pet22b  (Millipore)


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    Structured Review

    Millipore vector pet22b
    Vector Pet22b, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pet22b/product/Millipore
    Average 89 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    vector pet22b - by Bioz Stars, 2020-08
    89/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Crystallization and preliminary X-ray crystallographic analysis of Aquifex aeolicus SelA, a bacterial selenocysteine synthase
    Article Snippet: .. The mutant SelA gene corresponding to SelA-ΔN (amino-acid residues 62–452) was cloned into the Nde I and Xho I restriction sites of the vector pET22b (Novagen). .. The wild-type SelA encoded in the vector does not have any artificial sequences at its N- or C-termini, while SelA-ΔN only has an artificially introduced initiating Met residue followed by residues 62–452.

    Article Title: Protein sequences conserved in prokaryotic aminoacyl-tRNA synthetases are important for the activity of the processivity factor of human mitochondrial DNA polymerase
    Article Snippet: .. This product was cloned as an Nde I– Not I fragment in the vector pET22b(+) (Novagen) to generate clone pJAC39. ..

    Article Title: Visualization of Myelin Basic Protein (Mbp) T Cell Epitopes in Multiple Sclerosis Lesions Using a Monoclonal Antibody Specific for the Human Histocompatibility Leukocyte Antigen (Hla)-Dr2-Mbp 85-99 Complex
    Article Snippet: .. Recombinant human MBP was expressed in Escherichia coli using a cDNA construct with a COOH-terminal (His)6 -tag cloned into vector pET22b (Novagen). .. Expression was induced by addition of isopropyl-β-d -thiolactopyranoside (IPTG; 1 mM) to the growth media.

    Affinity Chromatography:

    Article Title: CheA-Receptor Interaction Sites in Bacterial Chemotaxis
    Article Snippet: .. The gene encoding CheAΔ354 (P4-P5 domain, 355-671) was subcloned into the vector pET22b (Novagen), and the expressed protein with a C-terminal His6 tag was purified using nickel-NTA affinity chromatography (Qiagen) and FPLC (Pharmacia). .. TM001490-206 construct and its purification were previously described in Vu et al .

    Article Title: The Linker between the Dimerization and Catalytic Domains of the CheA Histidine Kinase Propagates Changes in Structure and Dynamics That Are Important for Enzymatic Activity
    Article Snippet: .. Protein Preparation Genes encoding P4 (residues 356–540), P4l (residues 346–540), and P3P4 (residues 290–540) of CheA from Thermotoga maritima were subcloned into vector pET22b (Novagen), and the expressed proteins with C-terminal His6 tags were purified using Ni-NTA affinity chromatography (Qiagen). .. Purified proteins were dialyzed against the buffer containing 50 mM Na2 HPO4 (pH7.4) and 20 mM NaCl and concentrated to a final concentration of 0.5–1.5 mM.

    Mutagenesis:

    Article Title: Crystallization and preliminary X-ray crystallographic analysis of Aquifex aeolicus SelA, a bacterial selenocysteine synthase
    Article Snippet: .. The mutant SelA gene corresponding to SelA-ΔN (amino-acid residues 62–452) was cloned into the Nde I and Xho I restriction sites of the vector pET22b (Novagen). .. The wild-type SelA encoded in the vector does not have any artificial sequences at its N- or C-termini, while SelA-ΔN only has an artificially introduced initiating Met residue followed by residues 62–452.

    Construct:

    Article Title: Characterization of a Soluble Ligand Binding Domain of the NMDA Receptor Regulatory Subunit NR3A
    Article Snippet: .. The resulting NR3A S1S2 construct was then inserted into a modified version of the vector pET22b(+) (Novagen, Madison, WI), with an N-terminal His8 SSGLVPRGS affinity tag and thrombin cleavage site at its N terminus. ..

    Article Title: Visualization of Myelin Basic Protein (Mbp) T Cell Epitopes in Multiple Sclerosis Lesions Using a Monoclonal Antibody Specific for the Human Histocompatibility Leukocyte Antigen (Hla)-Dr2-Mbp 85-99 Complex
    Article Snippet: .. Recombinant human MBP was expressed in Escherichia coli using a cDNA construct with a COOH-terminal (His)6 -tag cloned into vector pET22b (Novagen). .. Expression was induced by addition of isopropyl-β-d -thiolactopyranoside (IPTG; 1 mM) to the growth media.

    Purification:

    Article Title: CheA-Receptor Interaction Sites in Bacterial Chemotaxis
    Article Snippet: .. The gene encoding CheAΔ354 (P4-P5 domain, 355-671) was subcloned into the vector pET22b (Novagen), and the expressed protein with a C-terminal His6 tag was purified using nickel-NTA affinity chromatography (Qiagen) and FPLC (Pharmacia). .. TM001490-206 construct and its purification were previously described in Vu et al .

    Article Title: The Linker between the Dimerization and Catalytic Domains of the CheA Histidine Kinase Propagates Changes in Structure and Dynamics That Are Important for Enzymatic Activity
    Article Snippet: .. Protein Preparation Genes encoding P4 (residues 356–540), P4l (residues 346–540), and P3P4 (residues 290–540) of CheA from Thermotoga maritima were subcloned into vector pET22b (Novagen), and the expressed proteins with C-terminal His6 tags were purified using Ni-NTA affinity chromatography (Qiagen). .. Purified proteins were dialyzed against the buffer containing 50 mM Na2 HPO4 (pH7.4) and 20 mM NaCl and concentrated to a final concentration of 0.5–1.5 mM.

    Fast Protein Liquid Chromatography:

    Article Title: CheA-Receptor Interaction Sites in Bacterial Chemotaxis
    Article Snippet: .. The gene encoding CheAΔ354 (P4-P5 domain, 355-671) was subcloned into the vector pET22b (Novagen), and the expressed protein with a C-terminal His6 tag was purified using nickel-NTA affinity chromatography (Qiagen) and FPLC (Pharmacia). .. TM001490-206 construct and its purification were previously described in Vu et al .

    Modification:

    Article Title: Characterization of a Soluble Ligand Binding Domain of the NMDA Receptor Regulatory Subunit NR3A
    Article Snippet: .. The resulting NR3A S1S2 construct was then inserted into a modified version of the vector pET22b(+) (Novagen, Madison, WI), with an N-terminal His8 SSGLVPRGS affinity tag and thrombin cleavage site at its N terminus. ..

    Recombinant:

    Article Title: Visualization of Myelin Basic Protein (Mbp) T Cell Epitopes in Multiple Sclerosis Lesions Using a Monoclonal Antibody Specific for the Human Histocompatibility Leukocyte Antigen (Hla)-Dr2-Mbp 85-99 Complex
    Article Snippet: .. Recombinant human MBP was expressed in Escherichia coli using a cDNA construct with a COOH-terminal (His)6 -tag cloned into vector pET22b (Novagen). .. Expression was induced by addition of isopropyl-β-d -thiolactopyranoside (IPTG; 1 mM) to the growth media.

    Plasmid Preparation:

    Article Title: Crystallization and preliminary X-ray crystallographic analysis of Aquifex aeolicus SelA, a bacterial selenocysteine synthase
    Article Snippet: .. The mutant SelA gene corresponding to SelA-ΔN (amino-acid residues 62–452) was cloned into the Nde I and Xho I restriction sites of the vector pET22b (Novagen). .. The wild-type SelA encoded in the vector does not have any artificial sequences at its N- or C-termini, while SelA-ΔN only has an artificially introduced initiating Met residue followed by residues 62–452.

    Article Title: Comparing substrate specificity of two UDP-sugar Pyrophosphorylases and Efficient One-pot Enzymatic Synthesis of UDP-GlcA and UDP-GalA
    Article Snippet: .. E. coli DH5α and BL21 (DE3), as well as vector pET22b were purchased from EMD Millipore (Bilerica, MA). .. Vector pQE80Lwas purchased from Qiagen (Germantown, MD).

    Article Title: CheA-Receptor Interaction Sites in Bacterial Chemotaxis
    Article Snippet: .. The gene encoding CheAΔ354 (P4-P5 domain, 355-671) was subcloned into the vector pET22b (Novagen), and the expressed protein with a C-terminal His6 tag was purified using nickel-NTA affinity chromatography (Qiagen) and FPLC (Pharmacia). .. TM001490-206 construct and its purification were previously described in Vu et al .

    Article Title: Molecular Determinants of the Regioselectivity of Toluene/o-Xylene Monooxygenase from Pseudomonas sp. Strain OX1 ▿ sp. Strain OX1 ▿ †
    Article Snippet: .. Escherichia coli strains JM109 and CJ236 and vector pET22b(+) were from Novagen. .. Plasmid pBZ1260 ( ) used for the expression of the ToMO cluster was kindly supplied by P. Barbieri (Dipartimento di Biologia Strutturale e Funzionale, Università dell'Insubria, Varese, Italy).

    Article Title: The Linker between the Dimerization and Catalytic Domains of the CheA Histidine Kinase Propagates Changes in Structure and Dynamics That Are Important for Enzymatic Activity
    Article Snippet: .. Protein Preparation Genes encoding P4 (residues 356–540), P4l (residues 346–540), and P3P4 (residues 290–540) of CheA from Thermotoga maritima were subcloned into vector pET22b (Novagen), and the expressed proteins with C-terminal His6 tags were purified using Ni-NTA affinity chromatography (Qiagen). .. Purified proteins were dialyzed against the buffer containing 50 mM Na2 HPO4 (pH7.4) and 20 mM NaCl and concentrated to a final concentration of 0.5–1.5 mM.

    Article Title: Protein sequences conserved in prokaryotic aminoacyl-tRNA synthetases are important for the activity of the processivity factor of human mitochondrial DNA polymerase
    Article Snippet: .. This product was cloned as an Nde I– Not I fragment in the vector pET22b(+) (Novagen) to generate clone pJAC39. ..

    Article Title: Characterization of a Soluble Ligand Binding Domain of the NMDA Receptor Regulatory Subunit NR3A
    Article Snippet: .. The resulting NR3A S1S2 construct was then inserted into a modified version of the vector pET22b(+) (Novagen, Madison, WI), with an N-terminal His8 SSGLVPRGS affinity tag and thrombin cleavage site at its N terminus. ..

    Article Title: Visualization of Myelin Basic Protein (Mbp) T Cell Epitopes in Multiple Sclerosis Lesions Using a Monoclonal Antibody Specific for the Human Histocompatibility Leukocyte Antigen (Hla)-Dr2-Mbp 85-99 Complex
    Article Snippet: .. Recombinant human MBP was expressed in Escherichia coli using a cDNA construct with a COOH-terminal (His)6 -tag cloned into vector pET22b (Novagen). .. Expression was induced by addition of isopropyl-β-d -thiolactopyranoside (IPTG; 1 mM) to the growth media.

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  • News
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  • 92
    Millipore pet22b vector
    Pet22b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet22b vector/product/Millipore
    Average 92 stars, based on 215 article reviews
    Price from $9.99 to $1999.99
    pet22b vector - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Millipore pet22b
    Pet22b, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet22b/product/Millipore
    Average 94 stars, based on 346 article reviews
    Price from $9.99 to $1999.99
    pet22b - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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