Journal: European Journal of Immunology

Article Title: Phospholipase activity of acyloxyacyl hydrolase induces IL‐22‐producing CD1a‐autoreactive T cells in individuals with psoriasis

doi: 10.1002/eji.202149485

Figure Lengend Snippet: AOAH protein detected in lesional psoriatic skin . HE staining and immunofluorescence on cryosections of healthy and lesional skin with left panel showing healthy skin and the right representing psoriatic lesional skin, Bar 100 μm. Images were captured by bright field and confocal microscopy. H&E was performed on 7 μm cryosections of healthy skin and lesional skin (A). IF on healthy skin and lesional cryosections fixed in ice‐cold acetone (B, C). CD1a staining was performed with Dylight 488 (Green) conjugated secondary antibody for anti‐CD1a. AOAH staining with Dylight 594 (Red) conjugated secondary antibody on healthy and lesional skin (C). Tissues sections were mounted with mounting media containing DAPI. High AOAH in psoriatic skin compared to healthy skin was detected in five healthy and five patients with psoriasis (D).

Article Snippet: Secondary antibody staining for AOAH was done with VectaFluor Excel Amplified anti‐Rabbit IgG, Dylight 594 antibody kit (Vector Laboratories, DK‐1594) and for CD1a with VectaFluor anti‐Mouse IgG, Dylight 488 antibody kit (Vector Laboratories, DK‐2488) according to the manufacturer's instruction.

Techniques: Staining, Immunofluorescence, Confocal Microscopy

Journal: European Journal of Immunology

Article Title: Phospholipase activity of acyloxyacyl hydrolase induces IL‐22‐producing CD1a‐autoreactive T cells in individuals with psoriasis

doi: 10.1002/eji.202149485

Figure Lengend Snippet: AOAH expressed in immune cells with multilobed nuclei and coexpressed in cells positive for CD1a immunofluorescence (IF) for psoriatic lesional skin cryosections for AOAH and CD1a showing different AOAH + cells with multilobed nuclei, and lymphoid‐like, and CD1a + AOAH + coexpressing cells. IF was performed on 7 μm lesional psoriatic skin cryosections, with secondary antibodies for AOAH conjugated to Dylight 594 (Red) and secondaries antibody for CD1a conjugated to Dylight 488 (Green), BAR 10 μm. Images were captured by confocal microscopy. (A) AOAH + neutrophil‐like multilobed nuclei cells in lesional epidermis (white arrows), in addition other mononuclear cells (green arrows) were also AOAH positive. (B and C) AOAH and CD1a coexpressing cells possibly representing LC population in psoriatic lesional skin. The results for single and dual staining for AOAH and CD1a were consistantly observed in at least three patients with psoriasis in three independent experiments.

Article Snippet: Secondary antibody staining for AOAH was done with VectaFluor Excel Amplified anti‐Rabbit IgG, Dylight 594 antibody kit (Vector Laboratories, DK‐1594) and for CD1a with VectaFluor anti‐Mouse IgG, Dylight 488 antibody kit (Vector Laboratories, DK‐2488) according to the manufacturer's instruction.

Techniques: Immunofluorescence, Confocal Microscopy, Staining

Journal: Biology

Article Title: Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts

doi: 10.3390/biology10121318

Figure Lengend Snippet: FFAs do not affect the desmin expression pattern in human primary myoblasts. ( a ) SkMs were treated with FAs conjugated with BSA or BSA alone (control) before DAPI and desmin staining. ( b ) Fluorescence images from 3 sets of experiments were analyzed, as described in the legend to . The number of DAPI-positive FFA-treated cells was compared to that of control cells, which was set to 1 (c, dashed gray line). The data are presented as the mean ± SD of cell number (dotted bars). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparison test. p ≤ 0.05 (*). p ≤ 0.01 (**).

Article Snippet: After staining, cells were covered with a coverslip in mounting medium with DAPI (DK2488, Vector Laboratories).

Techniques: Expressing, Staining, Fluorescence