ut b1  (Proteintech)

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: The characteristics of primer sets and PCR conditions used to detect mRNA expression.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing, Sequencing

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: Expression of AQP5, UT-A1, and UT-B1 in skin tissue by immunohistochemistry. (a1), (c1) (×40); (b1), (b2), (b3), (b4) (×200); (a2), (c2), (a3), (c3), (a4), (c4), (a5), (b5), (c5) (×400). FS : full-thickness skin; PC : positive control; UP : uremic patients; NMC : normal controls; NGC : negative control.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing, Immunohistochemistry, Positive Control, Negative Control

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: Semiquantitative analysis of the expression of AQP5, UT-A1, and UT-B1. Comparison of AQP5, UT-A1, and UT-B1 protein expression in each group. ∗ P < 0.01; # P < 0.05.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: Expression of AQP5, UT-A1, and UT-B1 mRNA in human skin. ∗ P < 0.01; # P < 0.05.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing

Journal: BioMed Research International

Article Title: The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

doi: 10.1155/2017/8629783

Figure Lengend Snippet: The relative expression of AQP5, UT-A1, and UT-B1 protein in human skin. ∗ P < 0.01; # P < 0.05.

Article Snippet: They were then blocked in 5% skim milk in TBST for 2 h. The following antibodies were used: AQP5 (ab78486, Abcam), UT-A1 (GTX41973, GeneTex), UT-B1 (25962-1-AP, Proteintech Group Inc.), or GAPDH (ab8245, Abcam).

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer

doi: 10.3389/fphys.2017.00245

Figure Lengend Snippet: Normal bladder urothelium expresses UT-B2 isoform. (A) Schematic diagram of human SLC14A1 gene and two pairs of primers for RT-PCR. (B) RT-PCR. PCR amplification of UT-B2 and UT-B1 from normal bladder tissue cDNA. GAPDH was used as an internal control. (C) Western blot analysis of UT-B protein expression in human tissues. The pre-made human tissue blot was purchased from Protein Biotechnologies and probed with UT-B antibody.

Article Snippet: Gene specific primers for UT-B1, UT-B2, and GAPDH were designed to generate amplicons of length 100–200 nucleotides by using the Invitrogen Primer program.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Western Blot, Expressing

Journal: Frontiers in Physiology

Article Title: Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer

doi: 10.3389/fphys.2017.00245

Figure Lengend Snippet: Analysis of UT-B gene expression from bladder cancer patients. (A) Representative RT-PCR amplification of UT-B by two pairs of primers. BCa-1 to Bca-13 are fresh bladder cancer tissues (from total n = 24) collected from patients and used for RT-PCR (C1234: normal bladder cDNA, C1235: bladder cancer cDNA, NHU: normal human urothelial cell line, HTB-4 and HTB-9: bladder cancer derived cell lines). (B) Quantification of UT-B gene expression by real-time PCR. UT-B gene expression was assessed in normal and cancerous tissues by real-time PCR using gene-specific primers and SYBR Green detection. Gene expression was determined using the 2 ΔCt method with GAPDH as control gene. Relative mRNA levels of UT-B in cancer ( n = 16) were compared to the normal bladder tissues ( n = 5), where the expression was set to 1.0 (mean ± SD, compared to control ** P < 0.01; NS, no significance). (C) Schematic diagram of UT-B2, UT-B1 and UT-B1 Δ24 and the location of deletion in UT-B1. (D) Functional study of UT-B activity. UT-B cRNAs (2ng/cell) were injected into oocytes. Three days later, UT-B activity was assessed by water permeability assay by placing cells in hypo-osmolarity solution. The cell rupture time was counted ( n = 7–10 cells/group) and the significance was determined by ANOVA ( * p < 0.05, ** p < 0.01). UT-B protein expression was examined by western blot with UT-B antibody ( n = 10 cells/group).

Article Snippet: Gene specific primers for UT-B1, UT-B2, and GAPDH were designed to generate amplicons of length 100–200 nucleotides by using the Invitrogen Primer program.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Derivative Assay, Real-time Polymerase Chain Reaction, SYBR Green Assay, Functional Assay, Activity Assay, Injection, Permeability, Western Blot

Journal: Frontiers in Physiology

Article Title: Identification of a Novel UT-B Urea Transporter in Human Urothelial Cancer

doi: 10.3389/fphys.2017.00245

Figure Lengend Snippet: Glycosylation analysis of UT-B2, UT-B1, and UT-B1Δ24 and western blot analysis of UT-B protein expression from bladder cancer patients. (A) PNGase F treatment. The cell lysates of HEK293 cell transfected with UT-B2, UT-B1, and UT-B1Δ24 were treated with or without PNGase F at 37°C for 2 h. (B) Fresh bladder cancer tissues were lysed in RIPA buffer. Supernatants were collected. UT-B protein expression was examined by western blot with UT-B antibody. Recombinant UT-B1, UT-B2, and UT-B1Δ24 expressed in HEK293 cells were used as controls. The same membrane was stripped and re-probed with GAPDH antibody.

Article Snippet: Gene specific primers for UT-B1, UT-B2, and GAPDH were designed to generate amplicons of length 100–200 nucleotides by using the Invitrogen Primer program.

Techniques: Western Blot, Expressing, Transfection, Recombinant