Structured Review

Addgene inc us0hisb pyrf
Us0hisb Pyrf, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/us0hisb pyrf/product/Addgene inc
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
us0hisb pyrf - by Bioz Stars, 2020-08
91/100 stars

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Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
Article Snippet: .. Bacterial Selection Assays For positive selection, US0hisB-pyrF-, a gift from Scot Wolfe (Addgene plasmid # 12614), harboring the plasmid pACYC184 -tacP- ccdB -T2 (Supplementary Fig. ), which contains a chloramphenicol resistance gene, were used to perform positive selections for SpCas9 variants. .. To generate competent cells used for these assays, cells were transformed with the selection plasmid and then cultured with tetracycline (10 µg/mL, Sigma) and chloramphenicol (33 µg/mL, Sigma) to generate electrocompetent cells as previously described .

Plasmid Preparation:

Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
Article Snippet: .. Bacterial Selection Assays For positive selection, US0hisB-pyrF-, a gift from Scot Wolfe (Addgene plasmid # 12614), harboring the plasmid pACYC184 -tacP- ccdB -T2 (Supplementary Fig. ), which contains a chloramphenicol resistance gene, were used to perform positive selections for SpCas9 variants. .. To generate competent cells used for these assays, cells were transformed with the selection plasmid and then cultured with tetracycline (10 µg/mL, Sigma) and chloramphenicol (33 µg/mL, Sigma) to generate electrocompetent cells as previously described .

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    Addgene inc us0hisb pyrf
    Us0hisb Pyrf, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/us0hisb pyrf/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    us0hisb pyrf - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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